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Direct And Indirect Effects Of Microstructured Titanium Substrates On The Induction Of Mesenchymal Stem Cell Differentiation Towards The Osteoblast Lineage.

R. Olivares-Navarrete, S. Hyzy, D. Hutton, Christopher P. Erdman, M. Wieland, B. Boyan, Z. Schwartz
Published 2010 · Materials Science, Medicine

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Microstructured and high surface energy titanium substrates increase osseointegration in vivo. In vitro, osteoblast differentiation is increased, but effects of the surface directly on multipotent mesenchymal stem cells (MSCs) and consequences for MSCs in the peri-implant environment are not known. We evaluated responses of human MSCs to substrate surface properties and examined the underlying mechanisms involved. MSCs exhibited osteoblast characteristics (alkaline phosphatase, RUNX2, and osteocalcin) when grown on microstructured Ti; this effect was more robust with increased hydrophilicity. Factors produced by osteoblasts grown on microstructured Ti were sufficient to induce co-cultured MSC differentiation to osteoblasts. Silencing studies showed that this was due to signaling via alpha2beta1 integrins in osteoblasts on the substrate surface and paracrine action of secreted Dkk2. Thus, human MSCs are sensitive to substrate properties that induce osteoblastic differentiation; osteoblasts interact with these surface properties via alpha2beta1 and secrete Dkk2, which acts on distal MSCs.
This paper references
10.2106/JBJS.G.00499
Effect of micrometer-scale roughness of the surface of Ti6Al4V pedicle screws in vitro and in vivo.
Z. Schwartz (2008)
10.1016/J.BIOMATERIALS.2007.02.008
Osteogenic differentiation of rabbit mesenchymal stem cells in thermo-reversible hydrogel constructs containing hydroxyapatite and bone morphogenic protein-2 (BMP-2).
K. Na (2007)
10.1563/1548-1336(1999)025<0095:OPCRTC>2.3.CO;2
Osteoblast progenitor cell responses to characterized titanium surfaces in the presence of bone morphogenetic protein-atelopeptide type I collagen in vitro.
J. Ong (1999)
10.1002/(SICI)1097-4636(199804)40:1<1::AID-JBM1>3.0.CO;2-Q
Bone response to unloaded and loaded titanium implants with a sandblasted and acid-etched surface: a histometric study in the canine mandible.
D. Cochran (1998)
10.1016/j.cell.2006.06.044
Matrix Elasticity Directs Stem Cell Lineage Specification
A. Engler (2006)
Titanium surface roughness alters responsiveness of MG63 osteoblast-like cells to 1 alpha,25-(OH)2D3.
B. Boyan (1998)
10.2174/157488807780599220
Nanotopographical control of human osteoprogenitor differentiation.
M. Dalby (2007)
10.1034/J.1600-0501.2002.130204.X
The use of reduced healing times on ITI implants with a sandblasted and acid-etched (SLA) surface: early results from clinical trials on ITI SLA implants.
D. Cochran (2002)
10.1002/JBM.A.30320
High surface energy enhances cell response to titanium substrate microstructure.
G. Zhao (2005)
10.1007/s00223-001-1114-y
Osteoblast-Mediated Mineral Deposition in Culture is Dependent on Surface Microtopography
B. Boyan (2002)
10.1002/JBM.A.30518
Enhancing surface free energy and hydrophilicity through chemical modification of microstructured titanium implant surfaces.
F. Rupp (2006)
10.1016/j.mce.2009.06.002
Is Wnt signalling the final common pathway leading to bone formation?
F. Milat (2009)
10.1002/J.0022-0337.2003.67.8.TB03681.X
Understanding peri-implant endosseous healing.
J. Davies (2003)
10.1002/path.2469
Why are MSCs therapeutic? New data: new insight
A. Caplan (2009)
10.1016/J.INJURY.2006.08.021
Enhancing the osteoinductive properties of hydroxyapatite by the addition of human mesenchymal stem cells, and recombinant human osteogenic protein-1 (BMP-7) in vitro.
E. Tsiridis (2006)
10.1002/jbm.a.32542
The influence of surface energy on early adherent events of osteoblast on titanium substrates.
H. Lai (2010)
10.1111/j.1582-4934.2007.00103.x
Calcium phosphate surfaces promote osteogenic differentiation of mesenchymal stem cells
P. Müller (2008)
10.1089/scd.2008.0130
A comprehensive analysis of the dual roles of BMPs in regulating adipogenic and osteogenic differentiation of mesenchymal progenitor cells.
Q. Kang (2009)
The roles of Wnt signaling Modulators Dickkopf1 ( Dkk 1 ) and Dickkopf2 ( Dkk 2 ) and cell maturation State inOsteogenesis on microstructured titanium surfaces
R Olivares-Navarrete (2010)
10.1177/08959374990130011301
Implant Surface Characteristics Modulate Differentiation Behavior of Cells in the Osteoblastic Lineage
Z. Schwartz (1999)
10.1002/JBM.A.30025
Microrough implant surface topographies increase osteogenesis by reducing osteoclast formation and activity.
S. Lossdörfer (2004)
10.1016/J.BIOMATERIALS.2007.02.024
Requirement for both micron- and submicron scale structure for synergistic responses of osteoblasts to substrate surface energy and topography.
G. Zhao (2007)
10.1016/j.biomaterials.2009.03.047
Mechanisms regulating increased production of osteoprotegerin by osteoblasts cultured on microstructured titanium surfaces.
Z. Schwartz (2009)
Effect of chemically modified titanium surfaces on protein adsorption and osteoblast precursor cell behavior.
J. Protivínský (2007)
10.1073/pnas.0805420105
Integrin α2β1 plays a critical role in osteoblast response to micron-scale surface structure and surface energy of titanium substrates
R. Olivares-Navarrete (2008)
10.1034/J.1600-0501.1996.070306.X
Evaluation of an endosseous titanium implant with a sandblasted and acid-etched surface in the canine mandible: radiographic results.
D. Cochran (1996)
10.7892/BORIS.23146
Clinical performance of wide-body implants with a sandblasted and acid-etched (SLA) surface: results of a 3-year follow-up study in a referral clinic.
M. Bornstein (2007)
10.1111/j.1708-8208.2009.00148.x
Early loading of nonsubmerged titanium implants with a chemically modified sand-blasted and acid-etched surface: 6-month results of a prospective case series study in the posterior mandible focusing on peri-implant crestal bone changes and implant stability quotient (ISQ) values.
M. Bornstein (2009)
Enhancement of osteoblastic differentiation of mesenchymal stromal cells cultured by selective combination of bone morphogenetic protein-2 (BMP-2) and fibroblast growth factor-2 (FGF-2)
前川 尚宜 (2008)
10.1096/fasebj.4.13.2210157
Relationship of cell growth to the regulation of tissue‐specific gene expression during osteoblast differentiation
G. Stein (1990)
10.1016/J.BIOMATERIALS.2006.01.010
Osteoprogenitor response to semi-ordered and random nanotopographies.
M. Dalby (2006)
10.1002/(SICI)1097-4636(199801)39:1<77::AID-JBM10>3.0.CO;2-L
Titanium surface roughness alters responsiveness of MG63 osteoblast‐like cells to 1α,25‐(OH)2D3
B. Boyan (1998)
10.1016/J.COLSURFB.2006.02.016
Does the nanometre scale topography of titanium influence protein adsorption and cell proliferation?
K. Cai (2006)
10.1096/fasebj.5.7.1707019
Function follows form: generation of intracellular signals by cell deformation
P. Watson (1991)
10.1089/ten.tea.2007.0292
Additive effect of RGD coating to functionalized titanium surfaces on human osteoprogenitor cell adhesion and spreading.
D. Le Guillou-Buffello (2008)
10.1002/jcb.20886
Mesenchymal stem cells as trophic mediators
A. Caplan (2006)
10.1002/(SICI)1097-4636(199609)32:1<55::AID-JBM7>3.0.CO;2-O
Surface roughness modulates the local production of growth factors and cytokines by osteoblast-like MG-63 cells.
K. Kieswetter (1996)
10.1111/J.1600-0501.2005.01195.X
Osteoblast-like cells are sensitive to submicron-scale surface structure.
Ge Zhao (2006)
10.1016/S0142-9612(01)00259-9
Morphological behavior of osteoblast-like cells on surface-modified titanium in vitro.
Y. Yang (2002)
10.1007/S10856-006-0002-4
Wettability modification and the subsequent manipulation of protein adsorption on a Ti6Al4V alloy by means of CO2 laser surface treatment
L. Hao (2007)
10.1002/jbm.a.31994
Study of hydroxyapatite osteoinductivity with an osteogenic differentiation of mesenchymal stem cells.
L. Lin (2009)
10.1016/j.jsbmb.2006.12.083
Beta-1 integrins mediate substrate dependent effects of 1α,25(OH)2D3 on osteoblasts
Z. Schwartz (2007)
10.1016/J.BIOCEL.2003.08.016
The collagen receptor subfamily of the integrins.
D. J. White (2004)
10.1016/J.BIOMATERIALS.2004.06.035
Differential regulation of osteoblasts by substrate microstructural features.
O. Zinger (2005)
Early wound healing around endosseous implants: a review of the literature.
Sangeetha Raghavendra (2005)
10.1016/j.biomaterials.2009.11.071
The roles of Wnt signaling modulators Dickkopf-1 (Dkk1) and Dickkopf-2 (Dkk2) and cell maturation state in osteogenesis on microstructured titanium surfaces.
R. Olivares-Navarrete (2010)
10.1016/J.BIOMATERIALS.2006.02.022
Integrin beta1 silencing in osteoblasts alters substrate-dependent responses to 1,25-dihydroxy vitamin D3.
L. Wang (2006)
10.1016/J.BIOMATERIALS.2005.08.028
Osteoprogenitor response to defined topographies with nanoscale depths.
M. Dalby (2006)



This paper is referenced by
10.1016/j.biomaterials.2012.10.035
High content imaging in the screening of biomaterial-induced MSC behavior.
H.V. Unadkat (2013)
10.1016/j.actbio.2015.03.022
Enhanced differentiation of human osteoblasts on Ti surfaces pre-treated with human whole blood.
Brigitte S Kopf (2015)
10.1002/jbm.b.32870
Effects of different sterilization techniques and varying anodized TiO₂ nanotube dimensions on bacteria growth.
Kim M. Kummer (2013)
10.1155/2014/401859
RhoA Controls Wnt Upregulation on Microstructured Titanium Surfaces
S. Lumetti (2014)
10.1002/jbm.a.35018
Mesenchymal stem cell response to conformal sputter deposited calcium phosphate thin films on nanostructured titanium surfaces.
Mura M McCafferty (2014)
10.25772/BYH2-V730
Designing Biomimetic Implant Surfaces to Promote Osseointegration under Osteoporotic Conditions by Revitalizing Mechanisms Coupling Bone Resorption to Formation
Ethan M. Lotz (2019)
10.1533/9780857098603.3.250
Corrosion resistant coatings for dental implants
P. Silva-Bermudez (2013)
Bone quality and mesenchymal stromal cell capacity in total hip replacement: Significance for stem osseointegration measured by radiostereometric analysis
J. Alm (2016)
The influence of surface chemistry on osteogenic differentiation of Human Mesenchymal Stem Cells
F. Sousa (2014)
10.4172/2157-7633.1000233
Receptor Kinase AXL is Modulated in the Osteogenic Differentiation of Human Mesenchymal Stromal Cells on Modified Titanium Implant Surfaces
M. R. Khan (2014)
10.1088/1748-6041/9/4/045001
Enhanced MC3T3-E1 preosteoblast response and bone formation on the addition of nano-needle and nano-porous features to microtopographical titanium surfaces.
X-M Zhuang (2014)
Human mesenchymal stem cells as a tool for joint repair in rheumatoid arthritis.
Y. Tanaka (2015)
Osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface
Rozila Ismail (2014)
10.1016/B978-0-12-803581-8.10218-8
The Effect of Substrate Microtopography on Osseointegration of Titanium Implants
Z. Schwartz (2011)
10.1039/C4TB00856A
Growth and accelerated differentiation of mesenchymal stem cells on graphene oxide/poly-l-lysine composite films.
W. Qi (2014)
effects of hydrogenated TiO 2 nanotube arrays on protein adsorption and compatibility with osteoblast-like cells
Ran Lu (2018)
10.1371/journal.pone.0153231
Spontaneous Differentiation of Human Mesenchymal Stem Cells on Poly-Lactic-Co-Glycolic Acid Nano-Fiber Scaffold
K. Sonomoto (2016)
10.1146/annurev-bioeng-071811-150021
Biophysical cues and cell behavior: the big impact of little things.
Joshua Z. Gasiorowski (2013)
10.1089/ten.TEA.2010.0567
Osteoblasts on rod shaped hydroxyapatite nanoparticles incorporated PCL film provide an optimal osteogenic niche for stem cell differentiation.
Z. Lu (2011)
10.1111/clr.12178
Pro-osteogenic topographical cues promote early activation of osteoprogenitor differentiation via enhanced TGFβ, Wnt, and Notch signaling.
N. Chakravorty (2014)
10.22203/ECM.V024A04
The importance of WNT pathways for bone metabolism and their regulation by implant topography.
C. Galli (2012)
10.1002/9781119242642.CH8
Plasma Polymer Deposition: A Versatile Tool for Stem Cell Research
M. MacGregor-Ramiasa (2016)
10.22203/eCM.v038a14
Adhesion, integration and osteogenesis of human dental pulp stem cells on biomimetic implant surfaces combined with plasma derived products.
I. Irastorza (2019)
10.1016/j.jpor.2011.12.002
Bioactivity of nanostructure on titanium surface modified by chemical processing at room temperature.
S. Komasa (2012)
10.1016/J.SURFCOAT.2018.02.044
Effect of oxidation time on cytocompatibility of ultrafine-grained pure Ti in micro-arc oxidation treatment
L. Xu (2018)
10.1016/j.msec.2016.04.035
The effect of simulated inflammatory conditions on the surface properties of titanium and stainless steel and their importance as biomaterials.
A. Fonseca-García (2016)
10.1007/7651_2013_30
Mesenchymal stem cells and nano-structured surfaces.
Y. Zhou (2013)
10.1016/B978-0-12-803581-8.09829-5
4.9 Integrin-Activated Reactions to Metallic Implant Surfaces☆
K. Sagomonyants (2011)
10.1111/cid.12213
Early Osseointegration Events on Neoss® ProActive and Bimodal Implants: A Comparison of Different Surfaces in an Animal Model.
G. Grossi-Oliveira (2015)
10.1038/nrrheum.2010.210
Regenerative medicine: Are calcium phosphate ceramics 'smart' biomaterials?
B. Boyan (2011)
10.3390/ma11122520
Effect of Titanium Implants Coated with Radiation-Crosslinked Collagen on Stability and Osseointegration in Rat Tibia
Eun-Bin Bae (2018)
10.1039/C2JM31290E
Nanofibrous gelatin–silica hybrid scaffolds mimicking the native extracellular matrix (ECM) using thermally induced phase separation
B. Lei (2012)
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