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The Development Of Electrically Conductive Polycaprolactone Fumarate-polypyrrole Composite Materials For Nerve Regeneration.

M. B. Runge, M. Dadsetan, J. Baltrusaitis, A. Knight, T. Ruesink, Eric Lazcano, L. Lu, A. Windebank, M. Yaszemski
Published 2010 · Materials Science, Medicine

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Electrically conductive polymer composites composed of polycaprolactone fumarate and polypyrrole (PCLF-PPy) have been developed for nerve regeneration applications. Here we report the synthesis and characterization of PCLF-PPy and in vitro studies showing PCLF-PPy materials support both PC12 cell and dorsal root ganglia (DRG) neurite extension. PCLF-PPy composite materials were synthesized by polymerizing pyrrole in preformed PCLF scaffolds (M(n) 7,000 or 18,000 g mol(-1)) resulting in interpenetrating networks of PCLF-PPy. Chemical compositions and thermal properties were characterized by ATR-FTIR, XPS, DSC, and TGA. PCLF-PPy materials were synthesized with five different anions (naphthalene-2-sulfonic acid sodium salt (NSA), dodecylbenzenesulfonic acid sodium salt (DBSA), dioctyl sulfosuccinate sodium salt (DOSS), potassium iodide (I), and lysine) to investigate effects on electrical conductivity and to optimize chemical composition for cellular compatibility. PCLF-PPy materials have variable electrical conductivity up to 6 mS cm(-1) with bulk compositions ranging from 5 to 13.5 percent polypyrrole. AFM and SEM characterization show microstructures with a root mean squared (RMS) roughness of 1195 nm and nanostructures with RMS roughness of 8 nm. In vitro studies using PC12 cells and DRG show PCLF-PPy materials synthesized with NSA or DBSA support cell attachment, proliferation, neurite extension, and are promising materials for future studies involving electrical stimulation.
This paper references
10.1021/BM060220Q
Carboxylic acid-functionalized conductive polypyrrole as a bioactive platform for cell adhesion.
Joo-Woon Lee (2006)
10.1002/JBM.A.31298
Methods for in vitro characterization of multichannel nerve tubes.
G. D. de Ruiter (2008)
10.1039/B611700G
A novel electrochemically synthesized biodegradable thin film of polypyrrole –polyethyleneglycol–polylactic acid nanoparticles
Galit Shustak (2007)
10.1016/j.expneurol.2007.12.023
Misdirection of regenerating motor axons after nerve injury and repair in the rat sciatic nerve model
G. C. D. Ruiter (2008)
10.1016/J.BIOMATERIALS.2006.12.007
Synthesis and characterization of electroactive and biodegradable ABA block copolymer of polylactide and aniline pentamer.
L. Huang (2007)
10.1021/bm7011828
Synthesis of biodegradable and electroactive multiblock polylactide and aniline pentamer copolymer for tissue engineering applications.
L. Huang (2008)
10.1080/02844310802393966
Implantation of Schwann cells in rat tendon autografts as a model for peripheral nerve repair: Long term effects on functional recovery
Hiroshi Arino (2008)
10.1098/rsif.2006.0141
Polypyrrole-based conducting polymers and interactions with biological tissues
D. D. Ateh (2006)
10.1023/A:1026799415199
Template synthesis of the polypyrrole tube and its bridging in vivo sciatic nerve regeneration
S. Chen (2000)
10.1016/j.nec.2008.07.026
Peripheral nerve: what's new in basic science laboratories.
J. Song (2009)
10.1002/JBM.A.30047
In vivo evaluation of a novel electrically conductive polypyrrole/poly(D,L-lactide) composite and polypyrrole-coated poly(D,L-lactide-co-glycolide) membranes.
Z. Wang (2004)
10.1016/j.actbio.2008.12.015
Photo-crosslinked poly(epsilon-caprolactone fumarate) networks for guided peripheral nerve regeneration: material properties and preliminary biological evaluations.
Shanfeng Wang (2009)
Accuracy of motor axon regeneration across autograft, single-lumen, and multi-channel poly(lactic-co-glycolic acid) nerve tubes. Neurosurgery 2008;63(1):144e55
GCd Ruiter (2008)
10.1007/S10856-007-3080-Z
Tissue spinal cord response in rats after implants of polypyrrole and polyethylene glycol obtained by plasma
R. Olayo (2008)
10.1109/JRPROC.1954.274680
Resistivity Measurements on Germanium for Transistors
L. Valdes (1954)
10.1016/J.BIOMATERIALS.2003.09.032
A novel electrically conductive and biodegradable composite made of polypyrrole nanoparticles and polylactide.
Guixin Shi (2004)
10.1016/J.BIOMATERIALS.2005.07.013
Synthesis and characterizations of biodegradable and crosslinkable poly(epsilon-caprolactone fumarate), poly(ethylene glycol fumarate), and their amphiphilic copolymer.
Shanfeng Wang (2006)
10.1002/JBM.A.31337
Electrical stimulation enhances viability of human cutaneous fibroblasts on conductive biodegradable substrates.
Guixin Shi (2008)
10.1002/JBM.A.30165
Surface chemistry mediates adhesive structure, cytoskeletal organization, and fusion of macrophages.
M. Dadsetan (2004)
10.1021/la8025683
Electrical pulsed stimulation of surfaces homogeneously coated with gold nanoparticles to induce neurite outgrowth of PC12 cells.
J. Park (2009)
10.1016/j.expneurol.2007.08.005
One hour electrical stimulation accelerates functional recovery after femoral nerve repair
P. Ahlborn (2007)
10.1073/PNAS.94.17.8948
Stimulation of neurite outgrowth using an electrically conducting polymer.
C. Schmidt (1997)
10.1227/01.NEU.0000335081.47352.78
Accuracy of motor axon regeneration across autograft, single-lumen, and multichannel poly(lactic-co-glycolic acid) nerve tubes.
G. D. de Ruiter (2008)
10.1039/B617697F
Reactions of sulfur dioxide on calcium carbonate single crystal and particle surfaces at the adsorbed water carbonate interface.
J. Baltrusaitis (2007)
10.1089/NEU.2004.21.1355
A global perspective on spinal cord injury epidemiology.
Alun Ackery (2004)
10.1016/j.biomaterials.2008.06.010
The regulation of cell functions electrically using biodegradable polypyrrole-polylactide conductors.
Guixin Shi (2008)
10.1016/j.neuint.2008.11.002
The role of biodegradable engineered scaffolds seeded with Schwann cells for spinal cord regeneration
H. Tabesh (2009)
10.1002/ADFM.200600669
Micropatterned Polypyrrole: A Combination of Electrical and Topographical Characteristics for the Stimulation of Cells.
N. Gómez (2007)
Advances in stem cell transplantation for spinal cord injury
Cui G-X (2008)
10.1021/CM950312E
Structure and degradation behavior of polypyrrole doped with sulfonate anions of different sizes subjected to undoping-redoping cycles
K. Neoh (1996)
10.1021/nl802859a
Electrical stimulation of neural stem cells mediated by humanized carbon nanotube composite made with extracellular matrix protein.
N. W. Kam (2009)
10.1097/PHM.0b013e31815e6370
The Incidence of Peripheral Nerve Injury in Extremity Trauma
C. Taylor (2008)
10.1016/S0142-9612(00)00344-6
Electrical stimulation alters protein adsorption and nerve cell interactions with electrically conducting biomaterials.
A. Kotwal (2001)
10.1002/mus.21020
Therapeutic stimulation of denervated muscles: The influence of pattern
Z. Ashley (2008)
10.1021/BM050206Y
Synthesis, material properties, and biocompatibility of a novel self-cross-linkable poly(caprolactone fumarate) as an injectable tissue engineering scaffold.
E. Jabbari (2005)
10.1016/J.BIOMATERIALS.2005.07.045
Multiple-channel scaffolds to promote spinal cord axon regeneration.
M. Moore (2006)
10.1002/JBM.A.31047
Nerve growth factor-immobilized polypyrrole: bioactive electrically conducting polymer for enhanced neurite extension.
N. Gómez (2007)
10.1002/J.1538-7305.1958.TB03883.X
Measurement of sheet resistivities with the four-point probe
F. M. Smits (1958)
10.1016/j.expneurol.2008.01.020
Immediate electrical stimulation enhances regeneration and reinnervation and modulates spinal plastic changes after sciatic nerve injury and repair
M. Vivó (2008)
10.1002/JBM.A.20065
Evaluation of biocompatibility of polypyrrole in vitro and in vivo.
Xioadong Wang (2004)
Synthesis, material properties, and biocompatibility of a novel self-crosslinkable poly(caprolactone fumarate) as an injectable tissue engineering scaffold. Biomacromolecules 2005;6(5):2503e11
E Jabbari (2005)
Synthesisofbiodegradable and electroactive multiblock polylactide and aniline pentamer copolymer for tissue engineering applications
HuangL (2008)
10.1016/j.expneurol.2007.01.040
Electrical stimulation promotes sensory neuron regeneration and growth-associated gene expression
N. Geremia (2007)
10.1016/j.colsurfb.2008.07.012
New biocompatible polypyrrole-based films with good blood compatibility and high electrical conductivity.
Chun Mao (2008)
10.1634/stemcells.2007-1022
Direct‐Current Electrical Field Guides Neuronal Stem/Progenitor Cell Migration
Lei Li (2008)
10.1063/1.2403937
Simple setup to measure electrical properties of polymeric films
R. K. Hiremath (2006)
10.1179/174313208X362505
Growth factor and stem cell enhanced conduits in peripheral nerve regeneration and repair
Stephen W. P. Kemp (2008)
10.1292/JVMS.70.353
In vitro differentiation of canine celiac adipose tissue-derived stromal cells into neuronal cells.
Ken Sago (2008)
10.1111/J.1525-1594.2007.00335.X
Electrically conductive biodegradable polymer composite for nerve regeneration: electricity-stimulated neurite outgrowth and axon regeneration.
Ze Zhang (2007)
10.1002/jcp.21431
Small applied electric fields guide migration of hippocampal neurons
L. Yao (2008)
10.1021/LA062129D
Carboxy-endcapped conductive polypyrrole: biomimetic conducting polymer for cell scaffolds and electrodes.
Joo-Woon Lee (2006)



This paper is referenced by
10.3390/POLYM5031115
Nanomembranes and Nanofibers from Biodegradable Conducting Polymers
E. Llorens (2013)
10.1039/C6TB01722C
Covalent crosslinking of graphene oxide and carbon nanotube into hydrogels enhances nerve cell responses.
Xifeng Liu (2016)
10.1002/PAT.3912
Aligned poly (glycolide‐lactide) fiber membranes with conducting polypyrrole
Huiling Guo (2017)
10.2147/IJN.S24073
Skeletal myotube formation enhanced by electrospun polyurethane carbon nanotube scaffolds
S. Sirivisoot (2011)
10.1016/j.actbio.2010.10.013
Material properties and electrical stimulation regimens of polycaprolactone fumarate-polypyrrole scaffolds as potential conductive nerve conduits.
P. Moroder (2011)
Development of PCL/Ibuprofen Tubes for Peripheral Nerve Regeneration
G. V. Salmoriaa (2016)
Silk fibroin-based conducting polymer composite electrodes and their use as electromechanical actuators
Isabella S. Romero (2013)
10.1089/ten.TEB.2012.0716
Dynamic manipulation of hydrogels to control cell behavior: a review.
Kanika Vats (2013)
10.1016/J.CERAMINT.2012.05.038
Carbon nanostructures as nerve scaffolds for repairing large gaps in severed nerves
F. Tavangarian (2012)
10.1088/1758-5090/aa68ed
A facile one-step strategy for development of a double network fibrous scaffold for nerve tissue engineering.
Nasim Golafshan (2017)
10.1111/aor.13485
Tissue engineering scaffolds in the treatment of brain disorders in geriatric patients.
A. A. Karimi Zarchi (2019)
10.1016/J.CCLET.2019.07.002
Fabrication of extracellular matrix-coated conductive polypyrrole-poly(l-lactide) fiber-films and their synergistic effect with (nerve growth factor)/(epidermal growth factor) on neurites growth
Ximing Pu (2020)
10.1080/00914037.2019.1605513
A review on electrically conducting polymer bionanocomposites for biomedical and other applications
Neelima Dubey (2020)
10.1016/J.POLYMERTESTING.2016.08.021
Manufacturing of PCL/SAg tubes by melt-extrusion for nerve regeneration: Structure and mechanical properties
G. Salmoria (2016)
10.1021/am301844c
Enhancing the interface in silk-polypyrrole composites through chemical modification of silk fibroin.
Isabella S. Romero (2013)
10.1016/J.PROGPOLYMSCI.2013.06.003
Biodegradable and electrically conducting polymers for biomedical applications
B. Guo (2013)
10.1002/adma.201202625
In vitro and in vivo near-infrared photothermal therapy of cancer using polypyrrole organic nanoparticles.
K. Yang (2012)
10.1007/s11706-014-0233-0
Conductive Au nanowires regulated by silk fibroin nanofibers
Bo-Ju Dong (2014)
10.1080/03008207.2018.1424145
VEGF-mediated angiogenesis and vascularization of a fumarate-crosslinked polycaprolactone (PCLF) scaffold
E. Wagner (2018)
10.1007/s12274-016-1330-4
General synthesis of high-performing magneto-conjugated polymer core–shell nanoparticles for multifunctional theranostics
H. Yan (2016)
10.1016/j.biomaterials.2011.07.029
Comparison of polymer scaffolds in rat spinal cord: a step toward quantitative assessment of combinatorial approaches to spinal cord repair.
Bingkun K. Chen (2011)
10.3389/fnmol.2016.00117
Polymerizing Pyrrole Coated Poly (l-lactic acid-co-ε-caprolactone) (PLCL) Conductive Nanofibrous Conduit Combined with Electric Stimulation for Long-Range Peripheral Nerve Regeneration
Jialin Song (2016)
10.1016/j.colsurfb.2019.01.041
Adverse effects of p-TSA-doped polypyrrole particulate exposure during zebrafish (Danio rerio) development.
K. M. Costa (2019)
10.1016/j.addr.2019.06.004
Enabling biodegradable functional biomaterials for the management of neurological disorders.
Dingying Shan (2019)
10.1007/s11706-012-0157-5
Scaffolds for central nervous system tissue engineering
J. He (2012)
10.1002/PI.5190
N‐oligo(3‐hydroxybutyrate) derivatized polypyrroles ‐ towards bioerobible conducting copolymers
A. Domagała (2016)
10.1177/0885328211402704
Review paper: Progress in the Field of Conducting Polymers for Tissue Engineering Applications
Anca-Dana Bendrea (2011)
10.1039/C0JM04335D
Materials for central nervous system regeneration: bioactive cues
Christiane B. Gumera (2011)
10.1021/BK-2011-1080.CH011
Polymeric biomaterials: A history of use in musculoskeletal regenerative and reconstructive medicine
Wen-bin Zhang (2011)
Novel Scaffolds for Spinal Cord Repair
M. Kraemer (2011)
10.1089/ten.tea.2016.0341
Chondrocyte Attachment, Proliferation, and Differentiation on Three-Dimensional Polycaprolactone Fumarate Scaffolds.
E. Wagner (2017)
10.1016/J.PROCIR.2015.11.014
Development of PCL/Ibuprofen Tubes for Peripheral Nerve Regeneration
G. Salmoria (2016)
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