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The Targeted Intracellular Delivery Of Cytochrome C Protein To Tumors Using Lipid-apolipoprotein Nanoparticles.

S. K. Kim, M. Foote, L. Huang
Published 2012 · Biology, Medicine

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Intracellular-acting therapeutic proteins offer a promising clinical alternative to extracellular-acting agents, but are limited in efficacy by their low permeability into the cell cytoplasm. We have developed a nanoparticle (NP) composed of lipid (DOTAP/DOPE) and apolipoprotein (APOA-I) to mediate the targeted delivery of intracellular-acting protein drugs to non-small cell lung tumors. NPs were produced with either GFP, a fluorescent model protein, or cytochrome C (cytC), an inducer of apoptosis in cancer cells. GFP and cytC were separately conjugated with a membrane permeable sequence (MPS) peptide and were admixed with DOPE/DOTAP nanoparticle formulations to enable successful protein loading. Protein-loaded NPs were modified with DSPE-PEG-Anisamide to enable specific NP targeting to the tumor site in a xenograft model. The resulting particle was 20-30 nm in size and exhibited a 64-75% loading efficiency. H460 cells treated with the PEGylated MPS-cytC-NPs exhibited massive apoptosis. When MPS-GFP-NPs or MPS-cytC-NPs were intravenously administered in H460 tumor bearing mice, a specific tumor targeting effect with low NP accumulation in the liver was observed. In addition, MPS-cytC-NP treatment provoked a tumor growth retardation effect in H460 xenograft mice. We conclude that our NP enables targeted, efficacious therapeutic protein delivery for the treatment of lung cancer.
This paper references
10.1002/JPS.21082
Engineering biodegradable polyester particles with specific drug targeting and drug release properties.
F. Mohamed (2008)
10.1016/0005-2736(95)80017-A
Fusion of cationic liposomes with mammalian cells occurs after endocytosis.
I. Wróbel (1995)
Poly(lactide-co-glycolide) microparticles as systems for controlled release of proteins -- preparation and characterization.
Aleksandra Porjazoska (2004)
10.2144/000112169
Functional reconstitution of Beta2-adrenergic receptors utilizing self-assembling Nanodisc technology.
Andrew J Leitz (2006)
10.1016/j.jconrel.2010.03.016
Stealth nanoparticles: high density but sheddable PEG is a key for tumor targeting.
Shyh-Dar Li (2010)
10.1002/ijc.20452
Anisamide‐targeted stealth liposomes: A potent carrier for targeting doxorubicin to human prostate cancer cells
R. Banerjee (2004)
Nanoparticles e a review
VJ Mohanraj (2006)
10.1038/sj.emboj.7601661
Nanodiscs unravel the interaction between the SecYEG channel and its cytosolic partner SecA
M. Alami (2007)
10.1517/17425247.2.1.29
Commercial challenges of protein drug delivery
L. Brown (2005)
10.1021/JA0393574
Directed self-assembly of monodisperse phospholipid bilayer Nanodiscs with controlled size.
I. Denisov (2004)
10.1042/BJ20101021
Effective siRNA delivery and target mRNA degradation using an amphipathic peptide to facilitate pH-dependent endosomal escape.
R. Bartz (2011)
Receptor mediated delivery of daunomycin using immunoliposomes: pharmacokinetics and tissue distribution in the rat.
J. Huwyler (1997)
10.1038/sj.onc.1207517
Role of AIF in caspase-dependent and caspase-independent cell death
S. Cregan (2004)
10.1128/AAC.50.4.1238-1244.2006
Nanodisk-Associated Amphotericin B Clears Leishmania major Cutaneous Infection in Susceptible BALB/c Mice
Keith G. Nelson (2006)
10.1021/pr800265f
Insertion of membrane proteins into discoidal membranes using a cell-free protein expression approach.
Federico Katzen (2008)
10.1007/s11095-009-0045-6
Stability of Protein Pharmaceuticals: An Update
M. Manning (2009)
10.1016/0092-8674(83)90291-X
Endocytosis of liposomes and intracellular fate of encapsulated molecules: Encounter with a low pH compartment after internalization in coated vesicles
R. Straubinger (1983)
10.1021/BI602371N
Applications of phospholipid bilayer nanodiscs in the study of membranes and membrane proteins.
Abhinav Nath (2007)
10.1016/J.JCONREL.2006.06.024
Cationic lipids, lipoplexes and intracellular delivery of genes.
L. Wasungu (2006)
10.1038/sj.onc.1208739
Novel inosine monophosphate dehydrogenase inhibitor VX-944 induces apoptosis in multiple myeloma cells primarily via caspase-independent AIF/Endo G pathway
K. Ishitsuka (2005)
Peptide stabilized amphotericin B nanodisks. Peptides 2007;28:741e6
M Tufteland (2007)
10.1016/j.febslet.2009.10.024
Membrane protein assembly into Nanodiscs
T. H. Bayburt (2010)
10.1016/j.peptides.2007.01.007
Peptide stabilized amphotericin B nanodisks
Megan Tufteland (2007)
10.1016/S0168-3659(03)00328-6
Polymer degradation and in vitro release of a model protein from poly(D,L-lactide-co-glycolide) nano- and microparticles.
J. Panyam (2003)
10.1021/BI962437I
Acyl chain unsaturation and vesicle curvature alter outer leaflet packing and promote poly(ethylene glycol)-mediated membrane fusion.
W. Talbot (1997)
10.1016/J.PROGPOLYMSCI.2007.04.001
Polymeric protein delivery systems
K. Lee (2007)
10.4314/TJPR.V5I1.14634
Nanoparticles - A Review
V. Mohanraj (2007)
10.1073/PNAS.84.21.7413
Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.
P. Felgner (1987)
10.1016/J.ADDR.2007.10.008
Tat peptide-mediated intracellular delivery of pharmaceutical nanocarriers.
V. Torchilin (2008)
10.1038/SJ.NEO.7900270
Defining characteristics of Types I and II apoptotic cells in response to TRAIL.
N. Ozören (2002)
10.1007/978-1-4684-4862-7_16
Endocytosis of Liposomes and Intracellular Fate of Encapsulated Molecules: Strategies for Enhanced Cytoplasmic Delivery
R. Straubinger (1984)
10.1016/j.bbamem.2010.02.013
Peptide-mediated protein delivery-which pathways are penetrable?
Helin Räägel (2010)
10.1093/RHEUMATOLOGY/KEH406
Extracellular cytochrome c, a mitochondrial apoptosis-related protein, induces arthritis.
R. Pullerits (2005)
10.1016/S0169-409X(97)00125-7
Protein release from gelatin matrices.
Ikada (1998)
10.1016/S0169-409X(01)00103-X
Roles of lipid polymorphism in intracellular delivery.
I. M. Hafez (2001)
fate of encapsulated molecules : encounter with a low pHcompartment after internalization in coatedvesicles
J Huwyler (1983)
10.1021/jp102074b
Static and dynamic characterization of nanodiscs with apolipoprotein A-I and its model peptide.
Masakazu Miyazaki (2010)
10.1146/annurev.biochem.052208.114057
Amphipols, nanodiscs, and fluorinated surfactants: three nonconventional approaches to studying membrane proteins in aqueous solutions.
J. Popot (2010)
10.1016/J.IJPHARM.2007.02.033
All-trans-retinoic acid nanodisks.
Katherine A Redmond (2007)
10.1016/j.bbamem.2009.06.022
Nanoparticles evading the reticuloendothelial system: role of the supported bilayer.
Shyh-Dar Li (2009)
10.1038/SJ.MT.6300323
Tumor-targeted delivery of siRNA by self-assembled nanoparticles.
Shyh-Dar Li (2008)



This paper is referenced by
The concentration of soluble receptors for TNF may serve as an additional predictor of the presence and clinical course of disease in patients with prostate
J. Li (2014)
10.1021/ACS.CHEMMATER.6B04404
Multifunctional Click Hyaluronic Acid Nanogels for Targeted Protein Delivery and Effective Cancer Treatment in Vivo
J. Chen (2016)
10.1002/adma.201902791
Rational Design of Nanocarriers for Intracellular Protein Delivery.
Xiaofei Qin (2019)
10.1007/s12195-017-0489-4
Phase-Separated Liposomes Enhance the Efficiency of Macromolecular Delivery to the Cellular Cytoplasm
Zachary I. Imam (2017)
Redox-Sensitive and Intrinsically Fluorescent Photoclick Hyaluronic Acid Nanogels for Traceable and Targeted Delivery of Cytochrome c to Breast Tumor in Mice
Shundong Li (2016)
10.1021/acsnano.5b07522
High-Density Lipoproteins: Nature's Multifunctional Nanoparticles.
Rui Kuai (2016)
Cytochrome C Purıfıcatıon With Surface Imprinted Bacterial Cellulose Nanofibers
E. T. Irmak (2014)
10.1002/PPSC.201400140
Advances in Anticancer Protein Delivery Using Micro-/ Nanoparticles.
W. Sun (2014)
10.1186/1471-2091-15-16
Chemical glycosylation of cytochrome c improves physical and chemical protein stability
Y. Delgado (2014)
10.1016/j.nano.2017.04.009
Effect of size and pegylation of liposomes and peptide-based synthetic lipoproteins on tumor targeting.
J. Tang (2017)
10.1021/acs.molpharmaceut.6b00461
Combining Stimulus-Triggered Release and Active Targeting Strategies Improves Cytotoxicity of Cytochrome c Nanoparticles in Tumor Cells.
Moraima Morales-Cruz (2016)
10.1007/s12257-019-0409-7
Encapsulation of Apoptotic Proteins in Lipid Nanoparticles to Induce Death of Cancer Cells
Chun-Sik Bae (2020)
10.1007/s11051-013-1498-4
Effect of DOPE and cholesterol on the protein adsorption onto lipid nanoparticles
G. Caracciolo (2013)
10.1016/J.JIEC.2017.09.039
Enhanced intracellular delivery of macromolecules by melittin derivatives mediated cellular uptake
Hei-Won Kyung (2018)
10.1039/c6nr03898k
Nanoparticles-cell association predicted by protein corona fingerprints.
S. Palchetti (2016)
10.3390/biom9040154
Targeted Delivery of Nanoparticulate Cytochrome C into Glioma Cells Through the Proton-Coupled Folate Transporter
Y. Kucheryavykh (2019)
10.1021/jacs.6b11934
Multifunctional Nanoparticles by Coordinative Self-Assembly of His-Tagged Units with Metal-Organic Frameworks.
Ruth Röder (2017)
10.1016/j.apsb.2017.11.006
Reconstituted high-density lipoproteins: novel biomimetic nanocarriers for drug delivery
X. Ma (2018)
10.7314/APJCP.2014.15.18.7617
Mechanism of fatty acid synthase in drug tolerance related to epithelial-mesenchymal transition of breast cancer.
J. Li (2014)
10.1039/C2SM27000E
The effect of using binary mixtures of zwitterionic and charged lipids on nanodisc formation and stability
Maria Wadsäter (2013)
10.1002/cphc.201501163
Cytochrome c-Capped Fluorescent Gold Nanoclusters: Imaging of Live Cells and Delivery of Cytochrome c.
Shyamtanu Chattoraj (2016)
10.1021/bm4007248
Galactose-decorated reduction-sensitive degradable chimaeric polymersomes as a multifunctional nanocarrier to efficiently chaperone apoptotic proteins into hepatoma cells.
X. Wang (2013)
10.1021/la401192x
Time evolution of nanoparticle-protein corona in human plasma: relevance for targeted drug delivery.
A. L. Barrán-Berdón (2013)
10.1039/C3BM60128E
Traceless protein delivery with an efficient recyclable nanocarrier.
Xuanjun Wu (2013)
10.1039/C4RA13335H
Lipid composition: a “key factor” for the rational manipulation of the liposome–protein corona by liposome design
G. Caracciolo (2015)
10.1039/C5RA15516A
Porous PLGA microparticles to encapsulate doxorubicin and polyethylenimine/miR-34a for inhibiting the proliferation and migration of lung cancer
C. Wang (2015)
10.1002/wnan.1615
Impact of the protein corona on nanomaterial immune response and targeting ability.
L. Digiacomo (2020)
10.1016/j.jconrel.2015.08.058
Efficacious delivery of protein drugs to prostate cancer cells by PSMA-targeted pH-responsive chimaeric polymersomes.
X. Li (2015)
10.3389/fphar.2017.00374
Asymmetrical Polymer Vesicles for Drug delivery and Other Applications
Y. Zhao (2017)
10.1016/j.actbio.2014.01.010
Reduction and pH dual-bioresponsive crosslinked polymersomes for efficient intracellular delivery of proteins and potent induction of cancer cell apoptosis.
H. Sun (2014)
10.1016/j.colsurfb.2014.01.001
Intracellular delivery and activation of the genetically encoded photosensitizer Killer Red by quantum dots encapsulated in polymeric micelles.
M. Muthiah (2014)
10.1002/chem.201303239
Engineering cytochrome-modified silica nanoparticles to induce programmed cell death.
Wen-Yen Huang (2013)
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