← Back to Search
Click Hydrogels, Microgels And Nanogels: Emerging Platforms For Drug Delivery And Tissue Engineering.
Yanjiao Jiang, J. Chen, C. Deng, E. Suuronen, Z. Zhong
Published 2014 · Materials Science, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
Hydrogels, microgels and nanogels have emerged as versatile and viable platforms for sustained protein release, targeted drug delivery, and tissue engineering due to excellent biocompatibility, a microporous structure with tunable porosity and pore size, and dimensions spanning from human organs, cells to viruses. In the past decade, remarkable advances in hydrogels, microgels and nanogels have been achieved with click chemistry. It is a most promising strategy to prepare gels with varying dimensions owing to its high reactivity, superb selectivity, and mild reaction conditions. In particular, the recent development of copper-free click chemistry such as strain-promoted azide-alkyne cycloaddition, radical mediated thiol-ene chemistry, Diels-Alder reaction, tetrazole-alkene photo-click chemistry, and oxime reaction renders it possible to form hydrogels, microgels and nanogels without the use of potentially toxic catalysts or immunogenic enzymes that are commonly required. Notably, unlike other chemical approaches, click chemistry owing to its unique bioorthogonal feature does not interfere with encapsulated bioactives such as living cells, proteins and drugs and furthermore allows versatile preparation of micropatterned biomimetic hydrogels, functional microgels and nanogels. In this review, recent exciting developments in click hydrogels, microgels and nanogels, as well as their biomedical applications such as controlled protein and drug release, tissue engineering, and regenerative medicine are presented and discussed.
This paper references
Hydrogel drug delivery system with predictable and tunable drug release and degradation rates
G. Ashley (2013)
Repair of bone defects using synthetic mimetics of collagenous extracellular matrices
M. Lutolf (2003)
Direct ″Click″ Synthesis of Hybrid Bisphosphonate–Hyaluronic Acid Hydrogel in Aqueous Solution for Biomineralization
X. Yang (2012)
Functional performance of human cardiosphere-derived cells delivered in an in situ polymerizable hyaluronan-gelatin hydrogel.
K. Cheng (2012)
Strain-Promoted Crosslinking of PEG-based Hydrogels via Copper-Free Cycloaddition.
Jukuan Zheng (2012)
Ultralow protein adsorbing coatings from clickable PEG nanogel solutions: benefits of attachment under salt-induced phase separation conditions and comparison with PEG/albumin nanogel coatings.
Casey D Donahoe (2013)
Cross-linking and degradation of step-growth hydrogels formed by thiol-ene photoclick chemistry.
Han M. Shih (2012)
New strategy for chemical modification of hyaluronic acid: preparation of functionalized derivatives and their use in the formation of novel biocompatible hydrogels.
P. Bulpitt (1999)
In situ forming hydrogels via catalyst-free and bioorthogonal "tetrazole-alkene" photo-click chemistry.
Yaping Fan (2013)
Influence of dialkyne structure on the properties of new click-gels based on hyaluronic acid.
Gabriella Testa (2009)
In situ gelling hydrogels for pharmaceutical and biomedical applications.
Sophie R. Van Tomme (2008)
A Diels-Alder modulated approach to control and sustain the release of dexamethasone and induce osteogenic differentiation of human mesenchymal stem cells.
K. C. Koehler (2013)
Bioorthogonal Click Chemistry: An Indispensable Tool to Create Multifaceted Cell Culture Scaffolds
Malar A. Azagarsamy (2013)
The in fl uence of matrix properties on growth and morphogenesis of human pancreatic ductal epithelial cells in 3 D
A Raza (2013)
Cell-responsive synthetic hydrogels
M. Lutolf (2003)
Enhanced proteolytic degradation of molecularly engineered PEG hydrogels in response to MMP-1 and MMP-2.
J. Patterson (2010)
Hyaluronic acid hydrogel as Nogo-66 receptor antibody delivery system for the repairing of injured rat brain: in vitro.
W. Tian (2005)
A photolabile hydrogel for guided three-dimensional cell growth and migration
Y. Luo (2004)
Spatially controlled simultaneous patterning of multiple growth factors in three-dimensional hydrogels.
R. Wylie (2011)
Sequential click reactions for synthesizing and patterning three-dimensional cell microenvironments
C. DeForest (2009)
Cytocompatible poly(ethylene glycol)-co-polycarbonate hydrogels cross-linked by copper-free, strain-promoted click chemistry.
J. Xu (2011)
New approaches in hydrogel synthesis -- Click chemistry: A review
A. Uliniuc (2012)
Interfacial thiol-ene photoclick reactions for forming multilayer hydrogels.
Han M. Shih (2013)
Hydrolytically degradable poly(ethylene glycol) hydrogel scaffolds with tunable degradation and mechanical properties.
S. Zustiak (2010)
Synthetically simple, highly resilient hydrogels. Biomacromolecules 2012;13:584e8
J Cui (2012)
Rapidly in situforming degradable hydrogels from dextran thiols through Michael addition. Biomacromolecules 2007;8:1548e56
C Hiemstra (2007)
Investigation into thiol-(meth)acrylate Michael addition reactions using amine and phosphine catalysts
Guang-Zhao Li (2010)
Reliable and efficient procedures for the conjugation of biomolecules through Huisgen azide-alkyne cycloadditions.
E. Lallana (2011)
Poly(vinyl alcohol)-based hydrogels formed by click chemistry. Macromolecules 2006;39:1709e18
DA Ossipov (2006)
Synthesis and characterization of in situ chitosan-based hydrogel via grafting of carboxyethyl acrylate.
M. Kim (2007)
Cover Picture: Efficiency and Fidelity in a Click‐Chemistry Route to Triazole Dendrimers by the Copper(I)‐Catalyzed Ligation of Azides and Alkynes (Angew. Chem. Int. Ed. 30/2004)
P. Wu (2004)
Production of heparin-functionalized hydrogels for the development of responsive and controlled growth factor delivery systems.
T. Nie (2007)
Student award for outstanding research winner in the Ph.D. category for the 9th World Biomaterials Congress, Chengdu, China, June 1-5, 2012: synthesis and application of photodegradable microspheres for spatiotemporal control of protein delivery.
Mark W. Tibbitt (2012)
Cartilage repair using hyaluronan hydrogel-encapsulated human embryonic stem cell-derived chondrogenic cells.
W. Toh (2010)
Synthetic matrix metalloproteinase-sensitive hydrogels for the conduction of tissue regeneration: Engineering cell-invasion characteristics
M. Lutolf (2003)
Diels-Alder Click cross-linked hyaluronic acid hydrogels for tissue engineering.
Chelsea M Nimmo (2011)
Synthesis and characterization of new injectable and degradable dextran-based hydrogels
J. Maia (2005)
Functionalization of hyaluronic acid with chemoselective groups via a disulfide-based protection strategy for in situ formation of mechanically stable hydrogels.
D. Ossipov (2010)
Preparation and swelling properties of “click” hydrogel from polyaspartamide derivatives using tri-arm PEG and PEG-co-poly(amino urethane) azides as crosslinking agents
N. Huynh (2013)
The furan/maleimide Diels–Alder reaction: A versatile click–unclick tool in macromolecular synthesis
A. Gandini (2013)
In situ cross-linkable high molecular weight hyaluronan-bisphosphonate conjugate for localized delivery and cell-specific targeting: a hydrogel linked prodrug approach.
Oommen P Varghese (2009)
Relative reactivity and selectivity of vinyl sulfones and acrylates towards the thiol–Michael addition reaction and polymerization
S. Chatani (2013)
Efficient construction of therapeutics, bioconjugates, biomaterials and bioactive surfaces using azide-alkyne "click" chemistry.
J. Lutz (2008)
Orthogonal Chemoselective Assembly of Hyaluronic Acid Networks and Nanogels for Drug Delivery
D. Ossipov (2013)
Recombinant protein-co-PEG networks as cell-adhesive and proteolytically degradable hydrogel matrixes. Part I: Development and physicochemical characteristics.
S. Rizzi (2005)
An in situ cross-linking hybrid hydrogel for controlled release of proteins.
Hila Epstein-Barash (2012)
Thiol-click chemistry: a multifaceted toolbox for small molecule and polymer synthesis.
Charles E. Hoyle (2010)
Hydrogels for protein delivery.
T. Vermonden (2012)
PEG hydrogels formed by thiol-ene photo-click chemistry and their effect on the formation and recovery of insulin-secreting cell spheroids.
Chien-Chi Lin (2011)
Synthesis and applications of biomedical and pharmaceutical polymers via click chemistry methodologies.
M. V. van Dijk (2009)
DNA delivery from matrix metalloproteinase degradable poly(ethylene glycol) hydrogels to mouse cloned mesenchymal stem cells.
Yuguo Lei (2009)
Synthesis by AGET ATRP of degradable nanogel precursors for in situ formation of nanostructured hyaluronic acid hydrogel.
S. Bencherif (2009)
Biological hydrogel synthesized from hyaluronic acid, gelatin and chondroitin sulfate by click chemistry.
X. Hu (2011)
Glycosaminoglycan hydrogel films as bio-interactive dressings for wound healing.
K. R. Kirker (2002)
The effect of immobilized platelet derived growth factor AA on neural stem/progenitor cell differentiation on cell-adhesive hydrogels.
Yukie Aizawa (2008)
Development of Thermal and Photochemical Strategies for Thiol−Ene Click Polymer Functionalization
L. Campos (2008)
Cytocompatible Click-based Hydrogels with Dynamically-Tunable Properties Through Orthogonal Photoconjugation and Photocleavage Reactions
C. DeForest (2011)
Chemical remodelling of cell surfaces in living animals
Jennifer A. Prescher (2004)
Anti-inflammatory function of an in situ cross-linkable conjugate hydrogel of hyaluronic acid and dexamethasone.
Taichi Ito (2007)
Investigation of the Diels-Alder reaction as a cross-linking mechanism for degradable poly(ethylene glycol) based hydrogels.
S. Kirchhof (2013)
Designing Cell-Compatible Hydrogels for Biomedical Applications
D. Seliktar (2012)
Hydrazone self-crosslinking of multiphase elastin-like block copolymer networks.
U. Krishna (2012)
Synthesis and characterization of enzymatically biodegradable PEG and peptidebased hydrogels prepared by click chemistry. Biomacromolecules 2010;11: 1608e14
M van Dijk (2010)
Photoinitiated alkyne-azide click and radical cross-linking reactions for the patterning of PEG hydrogels.
Rodney T. Chen (2012)
In situ cross-linkable hyaluronic acid hydrogels prevent post-operative abdominal adhesions in a rabbit model.
Y. Yeo (2006)
Synthesis and evaluation of injectable, in situ crosslinkable synthetic extracellular matrices for tissue engineering.
X. Z. Shu (2006)
Patterning network structure to spatially control cellular remodeling and stem cell fate within 3-dimensional hydrogels.
Sudhir Khetan (2010)
Synthetic hydrogel platform for three-dimensional culture of embryonic stem cell-derived motor neurons.
D. McKinnon (2013)
Microfluidic synthesis of cell-type-specific artificial extracellular matrix hydrogels.
S. Allazetta (2013)
Efficiency and fidelity in a click-chemistry route to triazole dendrimers by the copper(i)-catalyzed ligation of azides and alkynes.
P. Wu (2004)
The performance of human mesenchymal stem cells encapsulated in cell-degradable polymer-peptide hydrogels.
Sarah E B Anderson (2011)
Synthesis and characterization of new injectable and degradable dextran-based hydrogels. Polymer 2005;46:9604e14
J Maia (2005)
Cu(I)-Catalyzed Huisgen Azide—Alkyne 1,3-Dipolar Cycloaddition Reaction in Nucleoside, Nucleotide, and Oligonucleotide Chemistry
F. Amblard (2010)
Direct synthesis of biodegradable polysaccharide derivative hydrogels through aqueous Diels-Alder chemistry.
H. Tan (2011)
Responsive culture platform to examine the influence of microenvironmental geometry on cell function in 3D.
April M Kloxin (2012)
Novel injectable biodegradable glycol chitosan-based hydrogels crosslinked by Michael-type addition reaction with oligo(acryloyl carbonate)-b-poly(ethylene glycol)-b-oligo(acryloyl carbonate) copolymers.
Yuexin Yu (2011)
Degradable, click poly(vinyl alcohol) hydrogels: characterization of degradation and cellular compatibility.
M. Alves (2012)
Injectable, mixed natural-synthetic polymer hydrogels with modular properties.
M. Patenaude (2012)
Hydrogels with time-dependent material properties enhance cardiomyocyte differentiation in vitro.
J. Young (2011)
Novel in situ forming, degradable dextran hydrogels by Michael addition chemistry : Synthesis, rheology, and degradation
C. Hiemstra (2007)
Synthesis of thermosensitive guar‐based hydrogels with tunable physico‐chemical properties by click chemistry
M. Tizzotti (2010)
Cu-catalyzed azide-alkyne cycloaddition.
M. Meldal (2008)
Rapidly in situ forming biodegradable robust hydrogels by combining stereocomplexation and photopolymerization.
C. Hiemstra (2007)
Maleimide cross-linked bioactive PEG hydrogel exhibits improved reaction kinetics and cross-linking for cell encapsulation and in situ delivery.
Edward A. Phelps (2012)
Three-dimensional hMSC motility within peptide-functionalized PEG-based hydrogels of varying adhesivity and crosslinking density.
K. A. Kyburz (2013)
Nanogel antigenic protein-delivery system for adjuvant-free intranasal vaccines.
T. Nochi (2010)
Designing materials to direct stem-cell fate
M. Lutolf (2009)
Characterization of valvular interstitial cell function in three dimensional matrix metalloproteinase degradable PEG hydrogels.
J. Benton (2009)
Modular 'click-in-emulsion' bone-targeted nanogels.
D. Heller (2013)
Peptide-Functionalized Click Hydrogels with Independently Tunable Mechanics and Chemical Functionality for 3D Cell Culture
C. DeForest (2010)
Smart Design of Stable Extracellular Matrix Mimetic Hydrogel: Synthesis, Characterization, and In Vitro and In Vivo Evaluation for Tissue Engineering
Oommen P. Oommen (2013)
Rapidly in situ-forming degradable hydrogels from dextran thiols through Michael addition.
C. Hiemstra (2007)
Mechanical Properties of End-Linked PEG/PDMS Hydrogels
J. Cui (2012)
Michael addition reactions in macromolecular design for emerging technologies
B. D. Mather (2006)
Poly(vinyl alcohol)-Based Hydrogels Formed by “Click Chemistry”
D. Ossipov (2006)
Recombinant protein-co-PEG networks as cell-adhesive and proteolytically degradable hydrogel matrixes. Part 1: development and physicochemical characteristics. Biomacromolecules 2005;6:1226e38
SC Rizzi (2005)
Micro fl uidic Synthesis of Cell-Type-Speci fi c Arti fi cial Extracellular Matrix Hydrogels
S. Allazetta (2013)
In situ crosslinkable hyaluronic acid hydrogels prevent post-operative abdominal adhesions in a rabbit model. Biomaterials 2006;27:4698e705
Y Yeo (2006)
Preparation and swelling properties of “click” hydrogel from polyaspartamide derivatives using tri-arm PEG and PEG-co-poly(amino urethane) azides as crosslinking agents. Polymer 2013;54:1341e9
Huynh N-T (2013)
Biodegradable poly(ethylene glycol)-peptide hydrogels with well-defined structure and properties for cell delivery.
Shao Qiong Liu (2009)
Production of heparin-containing hydrogels for modulating cell responses.
T. Nie (2009)
Hydrophilic and amphiphilic polyethylene glycol-based hydrogels with tunable degradability prepared by "click" chemistry.
V. Truong (2012)
AeschlimannD.New strategy for chemicalmodification of hyaluronic acid: preparation of functionalized derivatives and their use in the formation of novel biocompatible hydrogels
P Bulpitt (1999)
Three-dimensional Chemical Patterning of Transparent Hydrogels
J. Wosnick (2008)
“Click” chemistry for in situ formation of thermoresponsive P(NIPAAm‐co‐HEMA)‐based hydrogels
Xiao-Ding Xu (2008)
Thermoresponsive Microgel-Based Materials
L. Lyon (2009)
In situ crosslinkable hyaluronan hydrogels for tissue engineering.
Xiao Zheng Shu (2004)
Cell-mediated delivery of glucocorticoids from thiol-ene hydrogels.
C. Yang (2012)
A microgel construction kit for bioorthogonal encapsulation and pH-controlled release of living cells.
D. Steinhilber (2013)
Thiol–Ene Photopolymerizations Provide a Facile Method To Encapsulate Proteins and Maintain Their Bioactivity
Joshua D. McCall (2012)
Release of model proteins and basic fibroblast growth factor from in situ forming degradable dextran hydrogels.
C. Hiemstra (2007)
Novel hydrogels via click chemistry: synthesis and potential biomedical applications.
V. Crescenzi (2007)
Synthetically tractable click hydrogels for three-dimensional cell culture formed using tetrazinenorbornene chemistry. Biomacromolecules 2013;14:949e53
DL Alge (2013)
Thermoresponsive microgel-based materials.
L. A. Lyon (2009)
Thiol-ene Michael-type formation of gelatin/poly(ethylene glycol) biomatrices for three-dimensional mesenchymal stromal/stem cell administration to cutaneous wounds.
K. Xu (2013)
Network formation and degradation behavior of hydrogels formed by Michael-type addition reactions.
A. Metters (2005)
Fully defined in situ cross-linkable alginate and hyaluronic acid hydrogels for myocardial tissue engineering.
J. Dahlmann (2013)
Poly(ethylene glycol) hydrogels formed by thiol-ene photopolymerization for enzyme-responsive protein delivery.
A. A. Aimetti (2009)
Fibronectin functional domains coupled to hyaluronan stimulate adult human dermal fibroblast responses critical for wound healing.
K. Ghosh (2006)
Synthesis and physicochemical characterization of end-linked poly(ethylene glycol)-co-peptide hydrogels formed by Michael-type addition.
M. Lutolf (2003)
Characterization of protein release from hydrolytically degradable poly(ethylene glycol) hydrogels
S. Zustiak (2011)
Spatial and temporal control of the alkyne-azide cycloaddition by photoinitiated Cu(II) reduction.
Brian J. Adzima (2011)
An injectable thiol-acrylate poly(ethylene glycol) hydrogel for sustained release of methylprednisolone sodium succinate.
Christopher D. Pritchard (2011)
Sequential crosslinking to control cellular spreading in 3-dimensional hydrogels
Sudhir Khetan (2009)
Protein delivery from materials formed by self-selective conjugate addition reactions.
D. Elbert (2001)
A stepwise huisgen cycloaddition process: copper(I)-catalyzed regioselective "ligation" of azides and terminal alkynes.
V. Rostovtsev (2002)
Three-dimensional biochemical patterning of click-based composite hydrogels via thiolene photopolymerization.
B. D. Polizzotti (2008)
Endothelial cell guidance in 3D patterned scaffolds.
Yukie Aizawa (2010)
Synthesis of thermosensitive P(NIPAAm-co-HEMA)/cellulose hydrogels via “click” chemistry
J. Zhang (2009)
Synthesis and physicochemical characterization of end-linked poly(ethylene glycol)-co-peptide hydrogels formed by Michaeltype addition. Biomacromolecules 2003;4:713e22
MP Lutolf (2003)
Biointerface properties of core-shell poly(vinyl alcohol)-hyaluronic acid microgels based on chemoselective chemistry.
S. Kupal (2012)
Metal free thiol-maleimide 'Click' reaction as a mild functionalisation strategy for degradable polymers.
Ryan J. Pounder (2008)
Synthetically simple, highly resilient hydrogels.
J. Cui (2012)
Reversible maleimide-thiol adducts yield glutathione-sensitive poly(ethylene glycol)-heparin hydrogels.
Aaron D Baldwin (2013)
In situ cross-linkable hydrogel of hyaluronan produced via copper-free click chemistry.
A. Takahashi (2013)
Hyaluronic acid click hydrogels emulate the extracellular matrix.
S. Owen (2013)
Systematic modulation of Michael-type reactivity of thiols through the use of charged amino acids.
M. Lutolf (2001)
Prolongation of sciatic nerve blockade by in situ cross-linked hyaluronic acid.
X. Jia (2004)
Synthesis of well-defined hydrogel networks using click chemistry.
M. Malkoch (2006)
Biocompatible hydrogels by oxime Click chemistry.
G. Grover (2012)
Small peptide functionalized thiol-ene hydrogels as culture substrates for understanding valvular interstitial cell activation and de novo tissue deposition.
Sarah T Gould (2012)
Biopolymer-based microgels/nanogels for drug delivery applications
J. Oh (2009)
A “click-chemistry” approach to cellulose-based hydrogels
A. Koschella (2011)
Visible-light-mediated thiol-ene hydrogelation using eosin-Y as the only photoinitiator.
Han M. Shih (2013)
Synthesis and characterization of hyaluronic acid-poly(ethylene glycol) hydrogels via Michael addition: An injectable biomaterial for cartilage repair.
R. Jin (2010)
′Click′ Chemistry in Polymer and Materials Science
W. Binder (2007)
Repair of a calvarial defect with biofactor and stem cell-embedded polyethylene glycol scaffold.
A. Terella (2010)
Preparation of fluorescent organometallic porphyrin complex nanogels of controlled molecular structure via reverse-emulsion click chemistry.
Guodong Fu (2012)
The influence of matrix properties on growth and morphogenesis of human pancreatic ductal epithelial cells in 3D.
A. Raza (2013)
In situ gelling stimuli-sensitive block copolymer hydrogels for drug delivery.
Chaoliang He (2008)
Click Chemistry with Polymers, Dendrimers, and Hydrogels for Drug Delivery
E. Lallana (2012)
In Vivo Imaging of Membrane-Associated Glycans in Developing Zebrafish
Scott T Laughlin (2008)
Surfactant free preparation of biodegradable dendritic polyglycerol nanogels by inverse nanoprecipitation for encapsulation and release of pharmaceutical biomacromolecules.
D. Steinhilber (2013)
The development of microgels/nanogels for drug delivery applications
J. Oh (2008)
Formation of a novel heparin-based hydrogel in the presence of heparin-binding biomolecules.
Giyoong Tae (2007)
Detailed characterization of an injectable hyaluronic acid-polyaspartylhydrazide hydrogel for protein delivery
R. Zhang (2011)
Synthesis and characterization of enzymatically biodegradable PEG and peptide-based hydrogels prepared by click chemistry.
M. V. van Dijk (2010)
Controlled synthesis of cell-laden microgels by radical-free gelation in droplet microfluidics.
Torsten Rossow (2012)
Diels-Alder mediated controlled release from a poly(ethylene glycol) based hydrogel.
K. C. Koehler (2013)
Reductive alkylation of hyaluronic acid for the synthesis of biocompatible hydrogels by click chemistry.
Gloria Huerta-Ángeles (2012)
Modular Synthesis of Thermosensitive P(NIPAAm-co-HEMA)/β-CD Based Hydrogels via Click Chemistry.
Xiao-Ding Xu (2009)
Injectable hydrogels as unique biomedical materials.
Lin Yu (2008)
Recombinant protein-co-PEG networks as cell-adhesive and proteolytically degradable hydrogel matrixes. Part II: biofunctional characteristics.
S. Rizzi (2006)
Photoreversible Patterning of Biomolecules within Click-Based Hydrogels**
C. DeForest (2012)
Poly(ethylene glycol) hydrogels formed by thiol-ene photopolymerization for enzyme-responsive protein delivery. Biomaterials 2009;30:6048e54
AA Aimetti (2009)
Rapid cross-linking of elastin-like polypeptides with (hydroxymethyl)phosphines in aqueous solution.
D. W. Lim (2007)
Photopolymerized thermosensitive poly(HPMAlactate)-PEG-based hydrogels: effect of network design on mechanical properties, degradation, and release behavior.
R. Censi (2010)
Bone reservoir: Injectable hyaluronic acid hydrogel for minimal invasive bone augmentation.
E. Martínez-Sanz (2011)
Thermosensitive hydrogels synthesized by fast Diels–Alder reaction in water
Hong-liang Wei (2009)
A Versatile Synthetic Extracellular Matrix Mimic via Thiol-Norbornene Photopolymerization.
B. Fairbanks (2009)
Synthetically Tractable Click Hydrogels for Three-Dimensional Cell Culture Formed Using Tetrazine–Norbornene Chemistry
D. Alge (2013)
HYDROGELS IN DRUG DELIVERY: PROGRESS AND CHALLENGES
T. Hoare (2008)
Regenerative biomaterials that "click": simple, aqueous-based protocols for hydrogel synthesis, surface immobilization, and 3D patterning.
Chelsea M Nimmo (2011)
Modular approach to functional hyaluronic acid hydrogels using orthogonal chemical reactions.
D. Ossipov (2010)
A hydrogel prepared by in situ cross-linking of a thiol-containing poly(ethylene glycol)-based copolymer: a new biomaterial for protein drug delivery.
B. Qiu (2003)
Tuning drug release from smart microgel-hydrogel composites via cross-linking.
Daryl N. Sivakumaran (2013)
Development of a hybrid dextrin hydrogel encapsulating dextrin nanogel as protein delivery system.
Maria Molinos (2012)
Novel injectable neutral solutions of chitosan form biodegradable gels in situ.
A. Chenite (2000)
Enzyme-mediated fast in situ formation of hydrogels from dextran-tyramine conjugates.
R. Jin (2007)
Peptidotriazoles on solid phase: [1,2,3]-triazoles by regiospecific copper(i)-catalyzed 1,3-dipolar cycloadditions of terminal alkynes to azides.
C. W. Tornøe (2002)
Photoinitiated alkyneeazide click and radical cross-linking reactions for the patterning of PEG hydrogels. Biomacromolecules 2012;13:889e95
RT Chen (2012)
Thiol–enes: Chemistry of the past with promise for the future
C. Hoyle (2004)
This paper is referenced by
Strategies for Hyaluronic Acid-Based Hydrogel Design in Drug Delivery
S. Trombino (2019)
The past, present and future of hydrogels
T. Vermonden (2015)
Click-Crosslinked Injectable Gelatin Hydrogels.
Sandeep T. Koshy (2016)
Concepts for Developing Physical Gels of Chitosan and of Chitosan Derivatives
Pasquale Sacco (2018)
Cell-Encapsulating Hydrogel Puzzle: Polyrotaxane-Based Self-Healing Hydrogels.
Ik Sung Cho (2019)
AIEgens for biological process monitoring and disease theranostics.
X. Gu (2017)
Functional biomedical hydrogels for in vivo imaging.
Kewen Lei (2016)
Fully degradable protein nanocarriers by orthogonal photoclick tetrazole-ene chemistry for the encapsulation and release.
Keti Piradashvili (2017)
Bio-Orthogonal Cross-Linking Chemistry Enables In Situ Protein Encapsulation and Provides Sustained Release from Hyaluronic Acid Based Hydrogels.
A. Famili (2017)
Do the properties of gels constructed by interlinking triply-responsive microgels follow from those of the building blocks?
Dongdong Lu (2019)
Redox-Sensitive and Intrinsically Fluorescent Photoclick Hyaluronic Acid Nanogels for Traceable and Targeted Delivery of Cytochrome c to Breast Tumor in Mice.
S. Li (2016)
Cellulose-Based Hydrogels in Topical Drug Delivery : A Challenge in Medical Devices
A. Ribeiro (2018)
Poly(N-isopropylacrylamide) based thin microgel films for use in cell culture applications
I. Sanzari (2020)
pH‐Responsive, Light‐Triggered on‐Demand Antibiotic Release from Functional Metal–Organic Framework for Bacterial Infection Combination Therapy
Zhiyong Song (2018)
Development of orthogonally crosslinked thiol-ene hydrogels for encapsulation of pancreatic beta-cells
H. Shih (2016)
“Click”-Inspired Chemistry in Macromolecular Science: Matching Recent Progress and User Expectations
Pieter Espeel (2015)
Cationic Synthetic Long Peptides-Loaded Nanogels: An Efficient Therapeutic Vaccine formulation for induction of T-cell Responses.
Neda Kordalivand (2019)
Novel multi-responsive and sugarcane bagasse cellulose-based nanogels for controllable release of doxorubicin hydrochloride
Y. Pan (2021)
Optimized aqueous Kinugasa reactions for bioorthogonal chemistry applications.
D. A. Bilodeau (2020)
SPECIFIC DNA SEQUENCE RESPONISIVE DNA CROSSLINKED HYDROGEL AND ITS APPLICATIONS
Qi Zhang Huang (2017)
Three-Dimensional Hyaluronic Acid Hydrogel-Based Models for In Vitro Human iPSC-Derived NPC Culture and Differentiation.
S. Wu (2017)
Innovative Systems from Clickable Biopolymer-Based Hydrogels for Drug Delivery
C. García-Astrain (2017)
Rational Design and Development of Anisotropic and Mechanically Strong Gelatin-Based Stress Relaxing Hydrogels for Osteogenic/Chondrogenic Differentiation.
K. Dey (2019)
Collagen Hydrogels for Regenerative Medicine
Justina Pupkaite (2020)
Mechanical response of double-network gels with dynamic bonds under multi-cycle deformation
A. Drozdov (2018)
Biological Influence of Nonswelling Microgels on Cartilage Induction of Mouse Adipose-Derived Stem Cells
Z. Liu (2019)
3-D Non-UV Digital Printing of Hydrogel Microstructures by Optically Controlled Digital Electropolymerization
N. Liu (2015)
Fabrication of PEG-carboxymethylcellulose hydrogel by thiol-norbornene photo-click chemistry.
Sora Lee (2016)
Soft hydrazone crosslinked hyaluronan- and alginate-based hydrogels as 3D supportive matrices for human pluripotent stem cell-derived neuronal cells
Jennika Karvinen (2018)
In Vitro Evaluation of Anti-Aggregation and Degradation Behavior of PEGylated Polymeric Nanogels under In Vivo Like Conditions.
Y. Chen (2018)
Injectable and Degradable Poly(Oligoethylene glycol methacrylate) Hydrogels with Tunable Charge Densities as Adhesive Peptide-Free Cell Scaffolds
Emilia Bakaic (2017)
Tailoring size and release kinetics of κ/ι-hybrid carrageenan microgels via a surfactant-assisted technique
S. Rodríguez (2020)See more