Online citations, reference lists, and bibliographies.
← Back to Search

Vascular Integrin Immunoreactivity Is Selectively Lost On Capillaries During Rat Focal Cerebral Ischemia And Reperfusion

D. Burggraf, Andreas Trinkl, Jan Burk, Helge K. Martens, G. F. Hamann
Published 2008 · Medicine

Cite This
Download PDF
Analyze on Scholarcy
The alpha1-integrin cell adhesion molecules, the principal endothelial receptors for basal lamina (BL) components disappear during transient ischemia. The current study investigated the localization of integrins, the time dependency and vessel size selectivity in the normal rat brain before and after 3 h of cerebral ischemia (I3) and reperfusion (R). Additionally we looked for a correlation to the amount of extravasation and hemorrhage. In the normal brain, there was a clear immunoreactivity for the alpha1, alpha6, and beta1 integrins on the endothelial perivascular cells. After I3 followed by variable reperfusion intervals of 0, 9, and 24 h (R0, R9 and R24; respectively), the number of vessels and staining intensity indicating immunoreactivity in the ischemic area were compared with the contralateral side. The number of the beta1-immunoreactive capillaries was steadily decreasing with the reperfusion time: -12+/-5%, -15+/-7% and -43+/-8% at I3R0, I3R9 and I3R24 (all p<0.05). The beta1-staining intensity decreased homogeneously to -21% at I3R24 (p<0.05). Vascular staining for alpha1 was affected similarly. Interestingly, the alpha6-positive arterioles/venules were also reduced by -21% at I3R24 (p<0.05) in a diameter-selective way on vessels with diameters larger than 15 mum. The correlated break-down of the blood-brain-barrier was demonstrated by the significant rise of the extravasation of BSA from the perfusion solution as well as the increased hemorrhage after MCAO/R (hemoglobin: 103+/-4% versus 330+/-17%; BSA 101+/-3% versus 132+/-9% in I0R0 and I3R24, respectively). The prominent capillary vulnerability contributes significantly to the impairment of the microvascular integrity and after ischemia and reperfusion.
This paper references
Suppression of ICE and apoptosis in mammary epithelial cells by extracellular matrix
N. Boudreau (1995)
Regulation of MSCF receptors on microglia in the normal and injured mouse central nervous system: A quantitative immunofluorescence study using confocal laser microscopy
G. Raivich (1998)
Microglial Activation and Matrix Protease Generation During Focal Cerebral Ischemia
G. D. del Zoppo (2007)
Alpha 6.beta 1 integrin (laminin receptor) is down-regulated by tumor necrosis factor alpha and interleukin-1 beta in human endothelial cells.
P. Defilippi (1992)
rt-PA causes a dose-dependent increase in the extravasation of cellular and non-cellular blood elements after focal cerebral ischemia
D. Burggraf (2007)
Distribution of Integrin-like Immunoreactivity on Primate Brain Microvasculature
Hans-Peter Haring (1996)
Cell biology and molecular mechanisms of injury in ischemic acute renal failure.
A. M. Sheridan (2000)
Integrins inhibit LPS-induced DNA strand breakage in cultured lung endothelial cells.
D. Hoyt (1996)
Recombinant human tissue plasminogen activator protects the basal lamina in experimental focal cerebral ischemia.
D. Burggraf (2003)
Immunohistochemical localization of extracellular matrix proteins in human glioma, both in vivo and in vitro.
S. Chintala (1996)
Microvascular Basal Lamina Injury after Experimental Focal Cerebral Ischemia and Reperfusion in the Rat
G. Hamann (2002)
Plasminogen Activation in Focal Cerebral Ischemia and Reperfusion
T. Pfefferkorn (2000)
Cell-Extracellular Matrix Interaction in Glioma Invasion
R. Goldbrunner (1999)
Development of the blood-brain barrier
W. Risau (1990)
Integrins: a review of their structure and mechanisms of ligand binding.
D. Tuckwell (1993)
Integrin signaling revisited.
M. Schwartz (2001)
The collagen receptor integrins have distinct ligand recognition and signaling functions.
J. Heino (2000)
Rapid Disruption of an Astrocyte Interaction With the Extracellular Matrix Mediated by Integrin α6β4 During Focal Cerebral Ischemia/Reperfusion
S. Wagner (1997)
Integrin alphavbeta3 is expressed in selected microvessels after focal cerebral ischemia.
Y. Okada (1996)
Mechanisms of resistance to natural killer cell-mediated cytotoxicity in acute lymphoblastic leukemia.
A. Romanski (2005)
Microvascular basal lamina antigens disappear during cerebral ischemia and reperfusion.
G. F. Hamann (1995)
Hemorrhagic Transformation and Microvascular Integrity during Focal Cerebral Ischemia/Reperfusion
G. F. Hamann (1996)
Integrin family of cell adhesion molecules in the injured brain: Regulation and cellular localization in the normal and regenerating mouse facial motor nucleus
C. Kloss (1999)
Microvascular Basal Lamina Damage after Embolic Stroke in the Rat: Relationship to Cerebral Blood Flow
G. Hamann (2003)
Characterization of a type IV collagen major cell binding site with affinity to the alpha 1 beta 1 and the alpha 2 beta 1 integrins
P. Vandenberg (1991)
Periodic depression by N-methyl-N-nitrosourea of electrocortical activity in the cat brain
K. Hossmann (2005)
Modulation of extracellular matrix receptors (integrins) on human endothelial cells by cytokines.
P. Defilippi (1992)
Transfection and dye premarking of human and rat glioma cell lines affects adhesion, migration and proliferation.
R. Goldbrunner (1997)
Tissue-type plasminogen activator induces opening of the blood-brain barrier via the LDL receptor-related protein.
M. Yepes (2003)
Cerebrovascular injury in stroke
Y. Ding (2006)
Rapid Loss of Microvascular Integrin Expression during Focal Brain Ischemia Reflects Neuron Injury
M. Tagaya (2001)
Functional regulation of the human integrin VLA‐1 (CD49a/CD29) by divalent cations and stimulatory β1 antibodies
A. Luque (1994)
Lipoprotein receptor–mediated induction of matrix metalloproteinase by tissue plasminogen activator
X. Wang (2003)
Integrin-matrix interactions in the cerebral microvasculature.
G. D. del Zoppo (2006)
The role of cell adhesion molecules in ischemic acute renal failure.
B. Molitoris (1999)
Contributions of the extracellular and cytoplasmic domains of platelet-endothelial cell adhesion molecule-1 (PECAM-1/CD31) in regulating cell-cell localization.
J. Sun (2000)
Reversible middle cerebral artery occlusion without craniectomy in rats.
E. Longa (1989)

This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar