Gold Nanoparticles As A Colorimetric Sensor For Protein Conformational Changes.
Soonwoo Chah, Matthew R. Hammond, Richard N. Zare
Published 2005 · Chemistry, Medicine
Download PDFAnalyze on Scholarcy
Spherical gold nanoparticles and flat gold films are prepared in which yeast iso-1-cytochrome c (Cyt c) is covalently bound to the gold surface by a thiol group in the cystein 102 residue. Upon exposure to solutions of different pH, bound Cyt c unfolds at low pH and refolds at high pH. This conformational change causes measurable shifts in the color of the coated nanoparticle solutions detected by UV-VIS absorption spectroscopy and in the refractive index (RI) of the flat gold films detected by surface plasmon resonance (SPR) spectroscopy. Both experiments demonstrate the same trend with pH, suggesting the use of protein-covered gold nanoparticles as a simple colorimetric sensor for conformational change.
This paper references
2 – Methods for Synthesis of Colloidal Gold
Dean A. Handley (1989)
Self-assembled nanoparticle probes for recognition and detection of biomolecules.
Dustin J. Maxwell (2002)
Effect of ionic strength on the binding of alpha-chymotrypsin to nanoparticle receptors.
Ayush Verma (2004)
Effect of colloidal gold size on the conformational changes of adsorbed cytochrome c: probing by circular dichroism, UV-visible, and infrared spectroscopy.
Xiue Jiang (2005)
Biomaterial‐Nanoparticle Hybrid Systems: Synthesis, Properties, and Applications
Eugenii Katz (2005)
A DNA-based method for rationally assembling nanoparticles into macroscopic materials
Chad A. Mirkin (1996)
Changes in Oligonucleotide Conformation on Nanoparticle Surfaces by Modification with Mercaptohexanol
Sunho Park (2004)
The Optical Properties of Metal Nanoparticles: The Influence of Size, Shape, and Dielectric Environment
K. Lance Kelly (2003)
Classification of acid denaturation of proteins: intermediates and unfolded states.
Anthony L. Fink (1994)
In situ X-ray observation of pedal-like conformational change and dimerization of trans-cinnamamide in cocrystals with phthalic acid.
Shigeru Ohba (2001)
In Situ FTIR Spectroscopic Study of the Conformational Change of Isotactic Polypropylene during the Crystallization Process
Xinyuan Zhu (2001)
Surface plasmon resonance method for probing interactions in nanostructures: CdS nanoparticles linked to Au and Ag substrates by self-assembled hexanedithiol and aminoethanethiol monolayers
Eliza Hutter (2001)
Effect of macromolecular crowding on DNA:Au nanoparticle bioconjugate assembly.
Glenn P. Goodrich (2004)
Gold Nanoparticles: Assembly, Supramolecular Chemistry, Quantum‐Size‐Related Properties, and Applications Toward Biology, Catalysis, and Nanotechnology.
Marie-Christine Daniel (2004)
The barriers in protein folding
Tobin R. Sosnick (1994)
Kinetic mechanism of cytochrome c folding: involvement of the heme and its ligands.
Gülnur A. Elöve (1994)
Effect of pH on formation of a nativelike intermediate on the unfolding pathway of a Lys 73 --> His variant of yeast iso-1-cytochrome c.
Sheela Godbole (1999)
Interfacial protein adsorption and inactivation.
Ajit Sadana (1992)
Protein stability: functional dependence of denaturational Gibbs energy on urea concentration.
Rinki Gupta (1999)
Measuring denatured state energetics: deviations from random coil behavior and implications for the folding of iso-1-cytochrome c.
Sheela Godbole (2000)
Optical properties of selected elements
J. H. Weaver (2002)
Effective concentrations of amino acid side chains in an unfolded protein.
Karthik Muthukrishnan (1991)
Protein adsorption and inactivation on surfaces. Influence of heterogeneities
Ajit Sadana (1992)
Accelerated color change of gold nanoparticles assembled by DNAzymes for simple and fast colorimetric Pb2+ detection.
Juewen Liu (2004)
Denaturation and renaturation of self-assembled yeast iso-1-cytochrome c on Au.
Soonwoo Chah (2004)
Spectral properties and relaxation dynamics of surface plasmon electronic oscillations in gold and silver nanodots and nanorods
Stephan Link‡ and (1999)
Nanosphere Lithography: Surface Plasmon Resonance Spectrum of a Periodic Array of Silver Nanoparticles by Ultraviolet−Visible Extinction Spectroscopy and Electrodynamic Modeling
Traci R. Jensen (1999)
Nanostructured gold hollow microspheres prepared on dissolvable ceramic hollow sphere templates.
Soonwoo Chah (2002)
Interfacial phase transition of an environmentally responsive elastin biopolymer adsorbed on functionalized gold nanoparticles studied by colloidal surface plasmon resonance.
N. Vallis Nath (2001)
Absorption and Scat
C. F. Bohren (1998)
The effect of substrate metal on 2-aminoethanethiol and nanoparticle enhanced surface plasmon resonance imaging
Soonwoo Chah (2001)
Structural Basis of a Phototropin Light Switch
Shannon M Harper (2003)
This paper is referenced by
Effect of gold nanoparticle size and surface coating on human red blood cells
Nanomaterial Interfaces in Biology
Paolo Bergese (2013)
Development of double-pulse lasers ablation system for generating gold ion source under applying an electric field
Amjad Khalil (2015)
Historical Introduction to Gold Colloids, Clusters and Nanoparticles
D. Michael P. Mingos (2014)
Enhancing Nanoparticle-Based Visible Detection by Controlling the Extent of Aggregation
Seokwon Lim (2012)
Reactivity of engineered inorganic nanoparticles and carbon nanostructures in biological media
Neus G Bastús (2008)
Protein Identity and Environmental Parameters Determine the Final Physicochemical Properties of Protein-Coated Metal Nanoparticles
Inna Dewald (2015)
Gold Nanoparticles as a Direct and Rapid Sensor for Sensitive Analytical Detection of Biogenic Amines
Kholoud M.M. Abou El-Nour (2017)
Green synthesis of time-stable palladium nanoparticles using microfluidic devices
Esteban Gioria (2020)
Nanoparticles that "remember" temperature.
Rafal Klajn (2010)
Gold nanoparticles in chemical and biological sensing.
Krishnendu Saha (2012)
Insulin-coated gold nanoparticles: a plasmonic device for studying metal-protein interactions.
Munish Chanana (2011)
Colorimetric detection based on localised surface plasmon resonance of gold nanoparticles: Merits, inherent shortcomings and future prospects.
Yanlin Zhang (2016)
Colloidal Au nanoparticle-based "turn on" fluorescence imaging for in-gel protein detection.
Na Na (2014)
Aptamer-conjugated nanomaterials for bioanalysis and biotechnology applications.
Tao Chen (2011)
Electrochemical β(1→3)-d-glucan biosensors fabricated by immobilization of enzymes with gold nanoparticles on platinum electrode.
Dipali R. Bagal-Kestwal (2010)
Surface assembly and intracellular delivery of biomolecule conjugated nanomaterials
Joshua M. Kogot (2008)
Functionalized self-assembly of gold nanoparticles functionalized with amino acids and aleurone globular protein
Maria Tomoaia-Cotişel (2009)
Key significance of DNA-target size in lateral flow assay coupled with recombinase polymerase amplification.
Irina V. Safenkova (2020)
Conjugation of nano and quantum materials with bovine serum albumin (BSA) to study their biological potential
Suman Singh (2013)
Flexible colorimetric detection of mercuric ion by simply mixing nanoparticles and oligopeptides.
Jianjun Du (2011)
Microemulsion-based synthesis of nanocrystalline materials.
Ashok Kumar Ganguli (2010)
Effect of Chemical Binding of Doxorubicin Hydrochloride to Gold Nanoparticles, Versus Electrostatic Adsorption, on the In Vitro Drug Release and Cytotoxicity to Breast Cancer Cells
Gamal M. Zayed (2018)
Polypeptide-Based Nanoscale Materials
Daniel Aili (2008)
Growth Kinetics of Gold Nanoparticle Formation from Glycated Hemoglobin
Ashwathi Asha Madhavan (2020)
Vitis vinifera peel polyphenols stabilized gold nanoparticles induce cytotoxicity and apoptotic cell death in A431 skin cancer cell lines
Jesuthankaraj Grace Nirmala (2017)
Anionic 11-mercaptoundecanoic acid capped ZnO nanoparticles
Michaela Šimšíková (2013)
2.04 – DNA-Conjugated Nanomaterials for Bioanalysis
Yung-chih Chen (2011)
Narrow Absorption in ITO-Free Perovskite Solar Cells for Sensing Applications Analyzed through Electromagnetic Simulation
Mahmoud Hamdy Mohamed Elshorbagy (2019)
Pyrazinium thioacetate capped gold nanoparticles as Fe(III) sensor and Fe(III) marked anti-proliferating agent in human neuroblastoma cells.
Ayaz Anwar (2019)
Dense aqueous colloidal gold nanoparticles prepared from highly concentrated precursor solution.
Tetsuro Soejima (2011)
Revealing the radiative and non-radiative relaxation rates of the fluorescent dye Atto488 in a λ/2 Fabry-Pérot-resonator by spectral and time resolved measurements.
Alexander Konrad (2016)See more