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In Vitro Toxicity Of Iron Oxide Nanoparticle: Oxidative Damages On Hep G2 Cells.
L. Sadeghi, F. Tanwir, V. Yousefi Babadi
Published 2015 · Medicine, Biology
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During the past years many studies have been done highlighting the great need for a more thorough understanding of cell-iron oxide nanoparticle interactions. To improve our knowledge in this field, there is a great need for standardized protocols that would allow to comparing the cytotoxic potential of any Fe2O3-NP type with previously studied particles. Several approaches are reported that several parameters which are of great importance for Fe2O3 nanoparticle induced toxicity. Nanoparticles because of their very small size can pass through the cell membrane and can make oxidative damage in all parts of the cells such as mitochondria, membrane, DNA due to high surface area. This study focuses on acute cytotoxicity of reactive oxygen species and DNA damaging effects of mentioned nanoparticles. Results showed increase of the oxidative damage leads cells to the apoptosis, therefore reduced cell viability. It is interesting that all of the results are concentration and time dependent.
This paper references
Lactate dehydrogenase activity as an effect criterion in toxicity tests with Daphnia magna straus.
T. Diamantino (2001)
Oxidative stress in bacteria and protein damage by reactive oxygen species.
E. Cabiscol (2000)
Increased Bax/Bcl-2 ratio up-regulates caspase-3 and increases apoptosis in the thymus of patients with myasthenia gravis.
S. Salakou (2007)
Oxidative stress and apoptosis induced by nanosized titanium dioxide in PC12 cells.
Shi-chang Liu (2010)
Defective cell death signalling along the Bcl-2 regulated apoptosis pathway compromises Treg cell development and limits their functionality in mice
D. Tischner (2012)
Characteristics of γ-H2AX foci at DNA double-strand breaks sites
D. Pilch (2003)
Evaluation of Iron Oxide nanoparticles effects on tissue and Enzymes of Thyroid in Rats
V. Y. Babadi (2013)
Proinflammogenic Effects of Low-Toxicity and Metal Nanoparticles In Vivo and In Vitro: Highlighting the Role of Particle Surface Area and Surface Reactivity
R. Duffin (2007)
Manganese nanoparticle activates mitochondrial dependent apoptotic signaling and autophagy in dopaminergic neuronal cells.
Hilary Afeseh Ngwa (2011)
Cytotoxic effect and apoptosis induction by silver nanoparticles in HeLa cells.
N. Miura (2009)
Magnetic nanoparticles in MR imaging and drug delivery.
C. Sun (2008)
ZnO nanorod-induced apoptosis in human alveolar adenocarcinoma cells via p53, survivin and bax/bcl-2 pathways: role of oxidative stress.
M. Ahamed (2011)
Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells.
Soohee Kim (2009)
In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride.
G. Fotakis (2006)
Particokinetics and extrapulmonary translocation of intratracheally instilled ferric oxide nanoparticles in rats and the potential health risk assessment.
Mo-Tao Zhu (2009)
Analytical and experimental study of residual stresses in CFRP
Chia-Chin Chiang (2013)
γ-H2AX - A Novel Biomarker for DNA Double-strand Breaks
Linda J. Kuo (2008)
Nanomaterials and nanoparticles: Sources and toxicity
C. Buzea (2007)
Oxidative Stress and Neurodegenerative Diseases: A Review of Upstream and Downstream Antioxidant Therapeutic Options
Bayani Uttara (2009)
Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications.
A. Gupta (2005)
Nanoparticle Uptake by the Rat Gastrointestinal Mucosa: Quantitation and Particle Size Dependency
P. Jani (1990)
Iron oxide magnetic nanoparticles: characterization and toxicity evaluation by in vitro and in vivo assays
A. Prodan (2013)
Oxidative stress and cerebral endothelial cells: regulation of the blood-brain-barrier and antioxidant based interventions.
L. Freeman (2012)
Magnetic nanoparticles: synthesis, protection, functionalization, and application.
A. Lu (2007)
Are nanoparticles potential male reproductive toxicants? A literature review
M. McAuliffe (2007)
Cellular Stress Responses
I. Milisav (2011)
Morphological and biochemical characterization and analysis of apoptosis.
R. T. Allen (1997)
An automated micro-fluorometric assay for monitoring oxidative burst activity of phagocytes.
C. Wan (1993)
A fluorometric method for determination of oxidized and reduced glutathione in tissues.
P. J. Hissin (1976)
Titanium dioxide induced cell damage: a proposed role of the carboxyl radical.
N. Dodd (2009)
Apoptosis: A Review of Programmed Cell Death
S. Elmore (2007)
Cytometric assessment of histone H2AX phosphorylation: a reporter of DNA damage.
X. Huang (2006)
Cell membrane damage and protein interaction induced by copper containing nanoparticles--importance of the metal release process.
H. Karlsson (2013)
ZnO nanoparticle-induced oxidative stress triggers apoptosis by activating JNK signaling pathway in cultured primary astrocytes
Jieting Wang (2014)
Cytotoxic Effect of Iron Oxide Nanoparticles on Mouse Embryonic Stem Cells by MTT Assay
Homa Mohseni Kouchesfehani (2013)
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L. S. Arias (2018)
Magnetic nanoparticles: reactive oxygen species generation and potential therapeutic applications
T. Mai (2017)
Macrophage functionality and homeostasis in response to oligoethyleneglycol-coated IONPs: Impact of a dendritic architecture.
Anne Casset (2019)
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Niluka M. Dissanayake (2015)
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Ioana Mihaela Popescu Din (2020)
Antioxidant effects of alfalfa can improve iron oxide nanoparticle damage: Invivo and invitro studies.
L. Sadeghi (2016)
Biosynthesis, characterization of magnetic iron oxide nanoparticles and evaluations of the cytotoxicity and DNA damage of human breast carcinoma cell lines
G. Sulaiman (2018)
The antibacterial and anticancer properties of zinc oxide coated iron oxide nanotextured composites.
T. Jana (2019)
Improving effects of Echium amoenum aqueous extract on rat model of Alzheimer's disease.
Leila Sadeghi (2018)
Toxicity of Metal Oxide Nanoparticles.
Koyeli Girigoswami (2018)
Oxidative Impact of Carob Leaf Extract–Synthesized Iron Oxide Magnetic Nanoparticles on the Kidney, Liver, Testis, and Spleen of Wistar Rats
H. Fahmy (2020)
Probing the toxicity of nanoparticles: a unified in silico machine learning model based on perturbation theory
R. Concu (2017)
Comparison of ultrasmall IONPs and Fe salts biocompatibility and activity in multi-cellular in vitro models
Natalia Janik-Olchawa (2020)
Cumulative effects of ciprofloxacin and pilocarpine on cytotoxicity and G0 phase arrest in hepatoma‐derived Hep G2 cell line
L. Sadeghi (2020)
Manganese dioxide nanoparticle induces Parkinson like neurobehavioral abnormalities in rats.
L. Sadeghi (2018)
Evaluation of Iron Oxide Nanoparticles Toxicity on Liver Cells of BALB/c Rats
K. Parivar (2016)
The presence of iron oxide nanoparticles in the food pigment E172.
Linn Voss (2020)
The effect of Fe2NiO4 and Fe4NiO4Zn magnetic nanoparticles on anaerobic digestion activity.
J. Chen (2018)
Tight junction between endothelial cells: the interaction between nanoparticles and blood vessels
Y. Zhang (2016)
Element Status in Rats at Intramuscular Injection of Iron Nanoparticles
Elena Sizova (2015)
Superparamagnetic Iron Oxide Nanoparticles in Musculoskeletal Biology
R. IyerShama (2016)
Metal-containing nanoparticles derived from concealed metal deposits: An important source of toxic nanoparticles in aquatic environments.
Guai Hu (2019)
Synthesis, Structural and Toxicity Characters of Nano-sized Titanium Dioxide/Magnetite Nanoparticles
Anita Listanti (2019)
Highly efficient mesenchymal stem cell proliferation on poly-ε-caprolactone nanofibers with embedded magnetic nanoparticles
J. Daňková (2015)
Toxicity of iron oxide nanoparticles: Size and coating effects
M. Abakumov (2018)
Toxicity Assessment of Nanomaterials
M. Tasso (2020)
Magnetic Hyperthermia and Oxidative Damage to DNA of Human Hepatocarcinoma Cells
Filippo Cellai (2017)
Environmental transformations and ecological effects of iron-based nanoparticles.
Cheng Lei (2018)
Nanoparticles and Genotoxicity
Tarek Gharsalli (2017)
Toxicity of metal and metal oxide nanoparticles: a review
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