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Effects Of Anti-inflammatory Drugs In Primary Kidney Cell Culture Of A Freshwater Fish.
J. Ribas, C. A. da Silva, L. D. de Andrade, G. Galvan, M. Cestari, E. Trindade, A. Zampronio, H. C. S. de Assis
Published 2014 · Biology, Medicine
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The non-steroidal anti-inflammatory drugs are emerging contaminants in aquatic ecosystems. This study aimed to evaluate toxic effects of some representative drugs of this pharmaceutical group on primary culture of monocytic lineage of Hoplias malabaricus anterior kidney. The effects of diclofenac, acetaminophen and ibuprofen in cell viability, lipopolysaccharide (LPS)-induced NO production and genotoxicity were evaluated. Cytometry analysis CD11b(+) cells showed 71.5% of stem cells, 19.5% of macrophages and 9% of monocytes. Cell viability was lower in the ficoll compared to percoll separation. LPS-induced NO production by these cells was blocked after treatment with dexamethasone and NG-Methyl-L-Arginine (L-NMMA). Exposure of the cells to diclofenac (0.2-200 ng/mL), acetaminophen (0.025-250 ng/mL) ibuprofen (10-1000 ng/mL) reduced basal NO production and inhibited LPS-induced NO production at all concentrations after 24 h of exposure. Genotoxicity occurred at the highest concentration of diclofenac and at the intermediary concentration of acetaminophen. Genotoxicity was also observed by ibuprofen. In summary, the pharmaceuticals influenced NO production and caused DNA damage in monocytic cells suggesting that these drugs can induce immunosuppression and genotoxicity in fish.
This paper references
Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids.
L. Green (1982)
Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.
T. Mosmann (1983)
Understanding cell toxicology : principles and practice
E. Walum (1990)
The kinetics of repair of oxidative DNA damage (strand breaks and oxidised pyrimidines) in human cells.
A. Collins (1995)
Inhibition of inducible nitric oxide synthase expression by novel nonsteroidal anti‐inflammatory derivatives with gastrointestinalsparing properties
G. Cirino (1996)
Inhibition of inducible nitric oxide synthase expression by novel nonsteroidal anti-inflammatory drugs with nitroxy moiety
G. Cirino (1996)
Key morphologic changes and DNA strand breaks in human lymphoid cells: discriminating apoptosis from necrosis.
D. Fairbairn (1996)
Comparative mutagenic and genotoxic effects of three propionic acid derivatives ibuprofen, ketoprofen and naproxen.
B. Philipose (1997)
Production of a macrophage growth factor(s) by a goldfish macrophage cell line and macrophages derived from goldfish kidney leukocytes.
N. Neumann (1998)
Peroxisome proliferator-activated receptor-gamma ligands reduce neuronal inducible nitric oxide synthase expression and cell death in vivo.
M. Heneka (2000)
Generation and functional analysis of distinct macrophage sub-populations from goldfish (Carassius auratus L.) kidney leukocyte cultures.
N. Neumann (2000)
Inflammatory Mechanisms in Alzheimer's Disease: Inhibition of β-Amyloid-Stimulated Proinflammatory Responses and Neurotoxicity by PPARγ Agonists
C. Combs (2000)
Acetaminophen inhibits NF-kappaB activation by interfering with the oxidant signal in murine Hepa 1-6 cells.
A. Boulares (2000)
Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing
R. Tice (2000)
Inflammatory mechanisms in Alzheimer's disease: inhibition of beta-amyloid-stimulated proinflammatory responses and neurotoxicity by PPARgamma agonists.
C. Combs (2000)
Peroxisome Proliferator-Activated Receptor-γ Ligands Reduce Neuronal Inducible Nitric Oxide Synthase Expression and Cell DeathIn Vivo
M. Heneka (2000)
Characterisation of growth enhancing factor production in different phases of in vitro fish macrophage development.
D. Barreda (2001)
Generation of primary monocyte-like cultures from rainbow trout head kidney leukocytes.
J. Stafford (2001)
Human pharmaceuticals in the aquatic environment a review.
O. Jones (2001)
Fármacos no meio ambiente
D. Bila (2003)
armacos no meio ambiente. Quím Nova 2003;26:523e30
DM Bila (2003)
Modulation of the activity of sea bass (Dicentrarchus labrax) head-kidney macrophages by macrophage activating factor(s) and lipopolysaccharide.
A. Sarmento (2004)
Nonsteroidal anti-inflammatory agents differ in their ability to suppress NF-kappaB activation, inhibition of expression of cyclooxygenase-2 and cyclin D1, and abrogation of tumor cell proliferation.
Y. Takada (2004)
The comet assay: a sensitive genotoxicity test for the detection of DNA damage.
G. Speit (2005)
Are pharmaceuticals potent environmental pollutants? Part I: environmental risk assessments of selected active pharmaceutical ingredients.
C. Carlsson (2006)
Ecotoxicology of human pharmaceuticals.
K. Fent (2006)
Pharmaceuticals in the Environment in Italy: Causes, Occurrence, Effects and Control
E. Zuccato (2006)
Amino acids and immune function
P. Li (2007)
Analysis of the effects diclofenac has on Japanese medaka (Oryzias latipes) using real-time PCR.
Han Na Hong (2007)
Human pharmaceuticals in the aquatic environment: a challenge to Green Chemistry.
Sushil K. Khetan (2007)
Probabilistic Estimates of Lifetime Daily Doses from Consumption of Drinking Water Containing Trace Levels of N,N-diethyl-meta-toluamide (DEET), Triclosan, or Acetaminophen and the Associated Risk to Human Health
D. Blanset (2007)
Exposure of Ancistrus multispinis (Regan, 1912, Pisces, Teleostei) to deltamethrin: effects on cellular immunity.
C. T. Pimpão (2008)
Exposure assessment of the pharmaceutical diclofenac based on long-term measurements of the aquatic input.
M. Letzel (2009)
Macrophage colony stimulating factor (CSF-1) is a central growth factor of goldfish macrophages.
Patrick C Hanington (2009)
An in vitro biomarker approach for the evaluation of the ecotoxicity of non-steroidal anti-inflammatory drugs (NSAIDs).
M. Parolini (2009)
The Comet assay for the evaluation of genotoxic impact in aquatic environments.
G. Frenzilli (2009)
Uptake and biological effects of environmentally relevant concentrations of the nonsteroidal anti-inflammatory pharmaceutical diclofenac in rainbow trout (Oncorhynchus mykiss).
A. C. Mehinto (2010)
Increased mitochondrial stress and modulation of mitochondrial respiratory enzyme activities in acetaminophen-induced toxicity in mouse macrophage cells.
Thekra Al-Belooshi (2010)
Toll-like receptor signaling in bony fish.
A. Rebl (2010)
Nephrotoxicity assessments of acetaminophen during zebrafish embryogenesis.
Hsi-Chu Peng (2010)
Evaluation of zebrafish DNA integrity after exposure to pharmacological agents present in aquatic environments.
L. Rocco (2010)
A new spiked sediment assay using embryos of the Japanese medaka specifically designed for a reliable toxicity assessment of hydrophobic chemicals.
Ludovic Vicquelin (2011)
Ibuprofen Genotoxicity in Aquatic Environment: An Experimental Model Using Oreochromis niloticus
Marilaine Ragugnetti (2011)
Heterogeneity of macrophage activation in fish.
M. Forlenza (2011)
First report about saxitoxins in freshwater fish Hoplias malabaricus through trophic exposure.
C. A. da Silva (2011)
Arginine and glutamine supplementation to culture media improves the performance of various channel catfish immune cells.
Camilo Pohlenz (2012)
Pharmaceuticals in Drinking Water
Aleksandra N. Jelić (2012)
Effects of anthraquinone extract from Rheum officinale Bail on the physiological responses and HSP70 gene expression of Megalobrama amblycephala under Aeromonas hydrophila infection.
B. Liu (2012)
Genotoxicity of the cancer chemopreventive drug candidates CP-31398, SHetA2, and phospho-ibuprofen.
Rupa S. Doppalapudi (2012)
Potentials and mechanisms of genotoxicity of six pharmaceuticals frequently detected in freshwater environment.
Xiaoshan Liu (2012)
Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat.
A. Luna-Acosta (2012)
Fish cell cultures as in vitro models of pro-inflammatory responses elicited by immunostimulants.
C. Fierro‐Castro (2012)
Effects of Ibuprofen on hematological, biochemical and enzymological parameters of blood in an Indian major carp, Cirrhinus mrigala.
M. Saravanan (2012)
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M. Almeida (2019)
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M. C. Perussolo (2019)
Pretreatment of carprofen impaired initiation of inflammatory‐ and overlapping resolution response and promoted cardiorenal syndrome in heart failure
V. Krishnan (2019)
Optimization of macrophage isolation from the Persian sturgeon and the Caspian kutum fish: a comparative study
S. Rastgar (2018)
In v i tro Effects of Lipopolysaccharide and Stress Hormones on Phagocytosis and Nitric Oxide Production by Enriched Head Kidney Macrophage Cultures in the Catfish Heteropneustes fossilis
R. Kumar (2018)
Photocatalytic Degradation of Commercial Acetaminophen: Evaluation, Modeling, and Scaling-Up of Photoreactors
Deyler R. Castilla-Caballero (2018)
Responses of Labeo rohita fingerlings to N-acetyl-p-aminophenol toxicity.
S. Renuka (2018)
Effects of low concentrations of ibuprofen on freshwater fish Rhamdia quelen.
Francielle Tatiana Mathias (2018)
Inhibition of immune responses and related proteins in Rhamdia quelen exposed to diclofenac.
João L.C. Ribas (2017)
Eco-pharmacovigilance of non-steroidal anti-inflammatory drugs: Necessity and opportunities.
Bingshu He (2017)
Evaluation of DNA damage and physiological responses in Nile tilapia, Oreochromis niloticus (Linnaeus, 1758) exposed to sub-lethal diclofenac (DCF).
P. Pandey (2017)
Degradation of diclofenac and triclosan residues in sewage sludge compost
E. Haiba (2017)
Amino acids as modulators of the European seabass, Dicentrarchus labrax, innate immune response: an in vitro approach
R. Azeredo (2017)
Optimization of Sewage Sludge Composting: Problems and Solutions. Reoveesette kompostimistehnoloogiate optimeerimine ravimijääkide kahjutustamise eesmärgil
L. Nei (2017)
Effects of trophic exposure to diclofenac and dexamethasone on hematological parameters and immune response in freshwater fish.
J. L. C. Ribas (2016)
Analytical determination and biochemical effects of waterborne diclofenac exposure to the common bully ( Gobiomorphus cotidianus)
Kerri-Anne B. Regan (2016)
Pharmaceutical residues and other emerging substances in the effluent of sewage treatment plants
C. Baresel (2015)
The primary culture of carp (Cyprinus carpio) macrophages and the verification of its phagocytosis activity
Wenhui Qiu (2015)