← Back to Search
Solid-State Protein Junctions: Cross-Laboratory Study ShowsPreservationof Mechanism At Varying Electronic Coupling
Sabyasachi Mukhopadhyay, S. K. Karuppannan, Cunlan Guo, Jerry A Fereiro, A. Bergren, Vineetha Mukundan, Xinkai Qiu, Olga E. Castañeda Ocampo, X. Chen, R. Chiechi, R. McCreery, I. Pecht, M. Sheves, Rupali Reddy Pasula, Sierin Lim, C. A. Nijhuis, A. Vilan, D. Cahen
Published 2020 · Medicine, Materials Science
Download PDFAnalyze on Scholarcy
Summary Successful integration of proteins in solid-state electronics requires contacting them in a non-invasive fashion, with a solid conducting surface for immobilization as one such contact. The contacts can affect and even dominate the measured electronic transport. Often substrates, substrate treatments, protein immobilization, and device geometries differ between laboratories. Thus the question arises how far results from different laboratories and platforms are comparable and how to distinguish genuine protein electronic transport properties from platform-induced ones. We report a systematic comparison of electronic transport measurements between different laboratories, using all commonly used large-area schemes to contact a set of three proteins of largely different types. Altogether we study eight different combinations of molecular junction configurations, designed so that Ageoof junctions varies from 105 to 10−3 μm2. Although for the same protein, measured with similar device geometry, results compare reasonably well, there are significant differences in current densities (an intensive variable) between different device geometries. Likely, these originate in the critical contact-protein coupling (∼contact resistance), in addition to the actual number of proteins involved, because the effective junction contact area depends on the nanometric roughness of the electrodes and at times, even the proteins may increase this roughness. On the positive side, our results show that understanding what controls the coupling can make the coupling a design knob. In terms of extensive variables, such as temperature, our comparison unanimously shows the transport to be independent of temperature for all studied configurations and proteins. Our study places coupling and lack of temperature activation as key aspects to be considered in both modeling and practice of protein electronic transport experiments.
This paper references
Measurement of electron transfer rates
A. K. Gaigalas (1997)
Influence of defects on the electrical characteristics of mercury-drop junctions: self-assembled monolayers of n-alkanethiolates on rough and smooth silver.
E. Weiss (2007)
A New Route to Nondestructive Top-Contacts for Molecular Electronics on Si: Pb Evaporated on Organic Monolayers.
R. Lovrincic (2013)
Charge transport and rectification in arrays of SAM-based tunneling junctions.
C. A. Nijhuis (2010)
Mechanism of Orientation-Dependent Asymmetric Charge Transport in Tunneling Junctions Comprising Photosystem I
Olga E. Castañeda Ocampo (2015)
Revealing tunnelling details by normalized differential conductance analysis of transport across molecular junctions.
A. Vilan (2017)
Nanoscale electron transport and photodynamics enhancement in lipid-depleted bacteriorhodopsin monomers.
Sabyasachi Mukhopadhyay (2014)
Tunneling Conductance of Asymmetrical Barriers
W. Brinkman (1970)
Charge transfer plasmon resonances across silver–molecule–silver junctions: estimating the terahertz conductance of molecules at near-infrared frequencies
L. Wu (2016)
Stretchable and Soft Electronics using Liquid Metals.
M. Dickey (2017)
Comparison of DC and AC Transport in 1.5-7.5 nm Oligophenylene Imine Molecular Wires across Two Junction Platforms: Eutectic Ga-In versus Conducting Probe Atomic Force Microscope Junctions.
C. S. S. Sangeeth (2016)
Equivalent circuits of a self-assembled monolayer-based tunnel junction determined by impedance spectroscopy.
C. S. S. Sangeeth (2014)
Investigation of protein adsorption and electrochemical behavior at a gold electrode.
S. Moulton (2003)
Rectification Ratio and Tunneling Decay Coefficient Depend on the Contact Geometry Revealed by in Situ Imaging of the Formation of EGaIn Junctions.
X. Chen (2019)
A brief history of molecular electronics.
M. Ratner (2013)
Fabrication and characterization of metal-molecule-metal junctions by conducting probe atomic force microscopy.
D. J. Wold (2001)
Mechanism of rectification in tunneling junctions based on molecules with asymmetric potential drops.
C. A. Nijhuis (2010)
Fabrication of reproducible, integration-compatible hybrid molecular/si electronics.
X. Yu (2014)
Iron-based ferritin nanocore as a contrast agent a)
Barindra Sana (2010)
Rethinking transition voltage spectroscopy within a generic Taylor expansion view.
A. Vilan (2013)
Temperature-dependent solid-state electron transport through bacteriorhodopsin: experimental evidence for multiple transport paths through proteins.
Lior Sepunaru (2012)
Metal–Insulator–Metal Junctions via Biomimetic Vesicle Fusion: Preparation, Characterization, and Biooptoelectronic Characteristics
K. P. Kommareddy (2010)
Bacteriorhodopsin‐Monolayer‐Based Planar Metal–Insulator–Metal Junctions via Biomimetic Vesicle Fusion: Preparation, Characterization, and Bio‐optoelectronic Characteristics
Y. Jin (2007)
Reversible Soft Top‐Contacts to Yield Molecular Junctions with Precise and Reproducible Electrical Characteristics
A. Wan (2014)
Biointerphases 5, 48, https://doi
S. Lim (2010)
conductance in biological molecules
L. Jiang (2014)
Interface Electrostatics Dictates the Electron Transport via Bioelectronic Junctions.
K. Garg (2018)
Conjugated Cofactor Enables Efficient Temperature-Independent Electronic Transport Across ∼6 nm Long Halorhodopsin.
Sabyasachi Mukhopadhyay (2015)
On-chip molecular electronic plasmon sources based on self-assembled monolayer tunnel junctions
Wei Du (2016)
Eutectic gallium-indium (EGaIn): a moldable liquid metal for electrical characterization of self-assembled monolayers.
R. Chiechi (2008)
Bacteriorhodopsin (bR) as an electronic conduction medium: current transport through bR-containing monolayers.
Y. Jin (2006)
X-ray study of the oxidation of liquid-gallium surfaces
M. J. Regan (1997)
Contacting organic molecules by soft methods: towards molecule-based electronic devices.
H. Haick (2008)
Solid-state electron transport via cytochrome c depends on electronic coupling to electrodes and across the protein
N. Amdursky (2014)
Experimental and Theoretical Analysis of Nanotransport in Oligophenylene Dithiol Junctions as a Function of Molecular Length and Contact Work Function.
Z. Xie (2015)
Electronic transport via proteins.
N. Amdursky (2014)
Charge transport in molecular electronic junctions: Compression of the molecular tunnel barrier in the strong coupling regime
S. Y. Sayed (2012)
Comprising Photosystem I
H. Hu (2019)
Comparison of the self‐chemisorption of azurin on gold and on functionalized oxide surfaces
B. Schnyder (2002)
Ultrasmooth and Photoresist‐Free Micropore‐Based EGaIn Molecular Junctions: Fabrication and How Roughness Determines Voltage Response
Senthil kumar Karuppannan (2019)
Transition from direct to inverted charge transport Marcus regions in molecular junctions via molecular orbital gating
L. Yuan (2018)
Uncovering a law of corresponding states for electron tunneling in molecular junctions.
I. Bâldea (2015)
Comparison of Electronic Transport Measurements on Organic Molecules
Adi Salomon (2003)
Statistical Tools for Analyzing Measurements of Charge Transport
W. Reus (2012)
Bacteriorhodopsin as an electronic conduction medium for biomolecular electronics.
Y. Jin (2008)
Soft Contact Deposition onto Molecularly Modified GaAs. Thin Metal Film Flotation: Principles and Electrical Effects
A. Vilan (2002)
All-carbon molecular tunnel junctions.
Haijun Yan (2011)
Analyzing Molecular Current-Voltage Characteristics with the Simmons Tunneling Model: Scaling and Linearization
A. Vilan (2007)
Fabrication and characterization of metal molecule metal junctions by conducting probe
D. J. Wold (2001)
Comparison of SAM-Based Junctions with Ga2O3/EGaIn Top Electrodes to Other Large-Area Tunneling Junctions
C. A. Nijhuis (2012)
Electron transport in molecular junctions
N. Tao (2006)
Low‐Voltage Current‐Voltage Relationship of Tunnel Junctions
J. Simmons (1963)
Protein Electronics: Chemical Modulation of Contacts Control Energy Level Alignment in Gold-Azurin-Gold Junctions.
Jerry A Fereiro (2018)
Electrical Conductance in Biological Molecules
M. Shinwari (2010)
Protein bioelectronics: a review of what we do and do not know.
Christopher D. Bostick (2018)
A Solid‐State Protein Junction Serves as a Bias‐Induced Current Switch
Jerry A Fereiro (2019)
Electrical conduction through single molecules and self-assembled monolayers
H. Akkerman (2008)
Insights into Solid-State Electron Transport through Proteins from Inelastic Tunneling Spectroscopy: The Case of Azurin.
X. Yu (2015)
Two stages in three-dimensional in vitro growth of tissue generated by osteoblastlike cells
K. Kommareddy (2010)
Large-Area, Ensemble Molecular Electronics: Motivation and Challenges.
A. Vilan (2017)
Concepts in photobiology : photosynthesis and photomorphogenesis
G. S. Singhal (1999)
Defining the value of injection current and effective electrical contact area for EGaIn-based molecular tunneling junctions.
F. C. Simeone (2013)
Electrical Resistance of AgTS–S(CH2)n−1CH3//Ga2O3/EGaIn Tunneling Junctions
L. Cademartiri (2012)
Proteins as solid-state electronic conductors.
I. Ron (2010)
Tuning electronic transport via hepta-alanine peptides junction by tryptophan doping
Cunlan Guo (2016)
Importance of monolayer quality for interpreting current transport through organic molecules: alkyls on oxide-free Si.
O. Seitz (2006)
Ambipolar transition voltage spectroscopy: Analytical results and experimental agreement
I. Bâldea (2012)
X-ray study of the oxidation of liquid-gallium
electronics. Nat (1997)
Long-Range Tunneling Processes across Ferritin-Based Junctions.
K. Kumar (2016)
A critical perspective on molecular electronic junctions: there is plenty of room in the middle.
R. McCreery (2013)
Defect Scaling with Contact Area in EGaIn-Based Junctions: Impact on Quality, Joule Heating, and Apparent Injection Current
L. Jiang (2015)
The true area of contact at a liquid metal‐solid interface
R. S. Timsit (1982)
Measurement of Electron Transfer Rates between Adsorbed Azurin and a Gold Electrode Modified with a Hexanethiol Layer
Electron Transfer and Adsorption of Myoglobin on Self-Assembled Surfactant Films: An Electrochemical Tapping-Mode AFM Study
S. Boussaad (1999)
Molecular Electronics : An Experimental and Theoretical Approach
I. Bâldea (2015)
Influence of the Contact Area on the Current Density across Molecular Tunneling Junctions Measured with EGaIn Top-Electrodes
P. Rothemund (2018)
This paper is referenced by