Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Microfluidic Immunomagnetic Cell Separation From Whole Blood.

Sajay Bhuvanendran Nair Gourikutty, Chia-pin Chang, P. D. Puiu
Published 2016 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Immunomagnetic-based separation has become a viable technique for the separation of cells and biomolecules. Here we report on the design and analysis of a simple and efficient microfluidic device for high throughput and high efficiency capture of cells tagged with magnetic particles. This is made possible by using a microfluidic chip integrated with customized arrays of permanent magnets capable of creating large magnetic field gradients, which determine the effective capturing of the tagged cells. This method is based on manipulating the cells which are under the influence of a combination of magnetic and fluid dynamic forces in a fluid under laminar flow through a microfluidic chip. A finite element analysis (FEA) model is developed to analyze the cell separation process and predict its behavior, which is validated subsequently by the experimental results. The magnetic field gradients created by various arrangements of magnetic arrays have been simulated using FEA and the influence of these field gradients on cell separation has been studied with the design of our microfluidic chip. The proof-of-concept for the proposed technique is demonstrated by capturing white blood cells (WBCs) from whole human blood. CD45-conjugated magnetic particles were added into whole blood samples to label WBCs and the mixture was flown through our microfluidic device to separate the labeled cells. After the separation process, the remaining WBCs in the elute were counted to determine the capture efficiency, and it was found that more than 99.9% WBCs have been successfully separated from whole blood. The proposed design can be used for positive selection as well as for negative enrichment of rare cells.
This paper references
10.1002/(SICI)1097-0320(19990801)36:4<294::AID-CYTO3>3.0.CO;2-C
Detection of rare MCF-7 breast carcinoma cells from mixtures of human peripheral leukocytes by magnetic deposition analysis.
B. Fang (1999)
10.1080/10408360590913696
DETECTION OF DISSEMINATED TUMOR CELLS IN PERIPHERAL BLOOD
V. Zieglschmid (2005)
10.1007/s10544-014-9856-2
Microfluidic platform for negative enrichment of circulating tumor cells
Bhuvanendran Nair Gourikutty Sajay (2014)
10.1007/S10404-004-0010-Y
Magnetic bead handling on-chip: new opportunities for analytical applications
M. Gijs (2004)
10.1007/S10404-012-1004-9
Continuous-flow ferrohydrodynamic sorting of particles and cells in microfluidic devices
Taotao Zhu (2012)
10.1039/B513005K
Magnetism and microfluidics.
N. Pamme (2006)
10.1088/0960-1317/15/12/012
Simulations of dynamic self-assembly of paramagnetic microspheres in confined microgeometries
D. Liu (2005)
10.1109/JMEMS.2010.2050194
A Microfluidic Device for Continuous-Flow Magnetically Controlled Capture and Isolation of Microparticles
Y. Zhou (2010)
10.1016/0968-0004(87)90152-6
Using magnetic orientation to study structure and assembly
J. Torbet (1987)
10.1023/B:BREA.0000036897.92513.72
Enumeration of Circulating Tumor Cells in the Blood of Breast Cancer Patients After Filtration Enrichment: Correlation with Disease Stage
H. Kahn (2004)
10.1007/S10404-008-0296-2
Customized trapping of magnetic particles
Q. Ramadan (2009)
10.1038/nature05058
The origins and the future of microfluidics
G. Whitesides (2006)
10.1186/1477-7819-3-18
Increase in number of circulating disseminated epithelial cells after surgery for non-small cell lung cancer monitored by MAINTRAC® is a predictor for relapse: A preliminary report
A. Rolle (2005)
10.1021/ac303284u
Circulating tumor cell microseparator based on lateral magnetophoresis and immunomagnetic nanobeads.
S. Kim (2013)
10.1023/A:1011490627871
Development and Characterization of Microfluidic Devices and Systems for Magnetic Bead-Based Biochemical Detection
Jin-Woo Choi (2001)
10.1063/1.1419049
Microelectromagnets for the control of magnetic nanoparticles
C. Lee (2001)
10.1016/j.aca.2011.02.019
Magnetoanalysis of micro/nanoparticles: a review.
M. Suwa (2011)
10.1007/s10544-013-9757-9
Towards an optimal and unbiased approach for tumor cell isolation
Bhuvanendran Nair Gourikutty Sajay (2013)
10.1002/elps.201000625
Interdigitated comb‐like electrodes for continuous separation of malignant cells from blood using dielectrophoresis
A. Alazzam (2011)
10.1063/1.372654
Diamagnetic levitation: Flying frogs and floating magnets (invited)
M. Simon (2000)
10.1063/1.4774311
Size-based hydrodynamic rare tumor cell separation in curved microfluidic channels.
J. Sun (2013)
10.1016/0014-5793(84)80814-5
Orientation of skeletal muscle actin in strong magnetic fields
J. Torbet (1984)
10.1039/c2lc40113d
Microfluidic magnetophoretic separations of immunomagnetically labeled rare mammalian cells.
T. P. Forbes (2012)
10.1007/978-3-642-59349-9_13
Detection of circulating tumor cells in blood using an optimized density gradient centrifugation.
R. Gertler (2003)
10.1007/s10544-009-9305-9
Microdevice for the isolation and enumeration of cancer cells from blood
S. J. Tan (2009)
10.1093/OXFORDJOURNALS.HUMREP.A138656
Trophoblasts circulating in maternal blood as candidates for prenatal genetic evaluation.
S. Yagel (1994)
10.1063/1.3668225
Erratum: “Computational design optimization for microfluidic magnetophoresis” [Biomicrofluidics 5, 013413 (2011)]
Brian D. Plouffe (2011)
10.1063/1.4821923
Rare cell isolation and profiling on a hybrid magnetic/size-sorting chip.
Jaehoon Chung (2013)
10.1063/1.3664092
Autonomous micro-magnet based systems for highly efficient magnetic separation
L. Zanini (2011)
10.1007/s10544-012-9718-8
Immunomagnetic nanoscreening of circulating tumor cells with a motion controlled microfluidic system
Y. Huang (2013)
10.1021/BI00286A020
Effect of magnetic susceptibility on nuclear magnetic resonance signals arising from red cells: a warning.
M. Fabry (1983)
10.1201/b18948
Introduction to Magnetism and Magnetic Materials
D. Jiles (2015)
10.1038/nature06385
Isolation of rare circulating tumour cells in cancer patients by microchip technology
S. Nagrath (2007)
10.1039/c1lc20270g
Microchip-based immunomagnetic detection of circulating tumor cells.
Kazunori Hoshino (2011)
10.1063/1.4825395
Microfluidic immunomagnetic cell separation using integrated permanent micromagnets.
O. Osman (2013)
10.1002/CYTO.10161
Comparison of two density gradient centrifugation systems for the enrichment of disseminated tumor cells in blood.
R. Rosenberg (2002)
10.1021/cr9001929
Microfluidic applications of magnetic particles for biological analysis and catalysis.
M. Gijs (2010)
10.1002/bit.22066
Optimization of an enrichment process for circulating tumor cells from the blood of head and neck cancer patients through depletion of normal cells
L. Yang (2009)
10.1080/14767050600676851
Sharpening the Tools: A summary of a National Institutes of Health workshop on new technologies for detection of fetal cells in maternal blood for early prenatal diagnosis
D. Bianchi (2006)
10.1063/1.3553239
Computational design optimization for microfluidic magnetophoresis.
Brian D. Plouffe (2011)
10.1093/ACPROF:OSO/9780199570447.003.0001
Introduction to Magnetism and Magnetic Materials
K. Krishnan (2016)
10.1073/PNAS.95.8.4589
Detection and characterization of carcinoma cells in the blood.
E. Racila (1998)
10.1109/TMAG.1983.1062795
Generalization of HGMS theory: The capture of ultra-fine particles
R. Gerber (1983)
10.1007/BF01907921
The measurement of blood density and its meaning
T. Kenner (2005)
10.1016/S0304-8853(00)01254-3
Optimization of ferrofluids and protocols for the enrichment of breast tumor cells in blood
P. Liberti (2001)
10.1073/pnas.0813188106
Isolating highly enriched populations of circulating epithelial cells and other rare cells from blood using a magnetic sweeper device
Amirali H. Talasaz (2009)
10.1039/c2lc20750h
Microsieve lab-chip device for rapid enumeration and fluorescence in situ hybridization of circulating tumor cells.
Li Shi Lim (2012)
10.1016/J.AJOG.2004.03.055
Automated detection of rare fetal cells in maternal blood: eliminating the false-positive XY signals in XX pregnancies.
M. Kilpatrick (2004)
10.1002/pd.1987
Detection of circulating fetal cells utilizing automated microscopy: potential for noninvasive prenatal diagnosis of chromosomal aneuploidies
A. Seppo (2008)
10.1088/0022-3727/36/13/201
TOPICAL REVIEW: Applications of magnetic nanoparticles in biomedicine
Q. Pankhurst (2003)
10.1021/mp9000519
Confocal images of circulating tumor cells obtained using a methodology and technology that removes normal cells.
P. Balasubramanian (2009)
10.1063/1.3576780
High-throughput size-based rare cell enrichment using microscale vortices.
Soojung Claire Hur (2011)
10.1039/c2lc40072c
A combined micromagnetic-microfluidic device for rapid capture and culture of rare circulating tumor cells.
J. H. Kang (2012)
10.1063/1.4774304
Antibody-independent isolation of circulating tumor cells by continuous-flow dielectrophoresis.
Sangjo Shim (2013)
10.1002/(SICI)1097-0223(199906)19:6<521::AID-PD578>3.0.CO;2-N
A comparison of different density gradients and antibodies for enrichment of fetal erythroblasts by MACS
C. Troeger (1999)



This paper is referenced by
10.1016/j.bios.2018.12.058
A flyover style microfluidic chip for highly purified magnetic cell separation.
Shujing Lin (2019)
10.1007/s10544-019-0378-9
Numerical evaluation and experimental validation of cross-flow microfiltration device design
Marisel De Jesús Vega (2019)
10.1126/sciadv.aba9628
Detection and isolation of free cancer cells from ascites and peritoneal lavages using optically induced electrokinetics (OEK)
Y. Zhang (2020)
10.3390/bioengineering4030067
Microfluidic Adaptation of Density-Gradient Centrifugation for Isolation of Particles and Cells
Y. Sun (2017)
10.1016/J.FOODCONT.2016.11.021
An automated system for separation and concentration of food-borne pathogens using immunomagnetic separation
Min-Cheol Lim (2017)
10.1063/1.5087690
Recent advances in microfluidic methods in cancer liquid biopsy.
F. S. Iliescu (2019)
10.3390/mi11030297
The Fabrication and Application Mechanism of Microfluidic Systems for High Throughput Biomedical Screening: A Review
Kena Song (2020)
10.1016/j.apmt.2019.100450
Recent trends on the development of systems for cancer diagnosis and treatment by microfluidic technology
Ana Cristina Alves Silva (2020)
10.1063/1.5035388
Recent advances and current challenges in magnetophoresis based micro magnetofluidics.
A. Munaz (2018)
10.3390/mi10100644
Continuous-Flow Separation and Efficient Concentration of Foodborne Bacteria from Large Volume Using Nickel Nanowire Bridge in Microfluidic Chip
Xiaoting Huo (2019)
10.3389/fbioe.2018.00192
Magnetic Force-Based Microfluidic Techniques for Cellular and Tissue Bioengineering
Sena Yaman (2018)
10.1186/s12967-016-1108-1
Advances in rare cell isolation: an optimization and evaluation study
Stefan Schreier (2016)
10.1016/j.jchromb.2018.04.046
Lateral fluid flow fractionation using dielectrophoresis (LFFF-DEP) for size-independent, label-free isolation of circulating tumor cells.
W. Waheed (2018)
10.1016/J.SNB.2018.07.176
Magnetophoretic separation of diamagnetic particles through parallel ferrofluid streams
A. Munaz (2018)
10.1016/J.JMMM.2016.11.002
Micromagnetic Cancer Cell Immobilization and Release for Real-Time Single Cell Analysis
D. Jaiswal (2017)
10.1007/s13367-016-0033-4
Numerical study on the complete blood cell sorting using particle tracing and dielectrophoresis in a microfluidic device
H. Ali (2016)
10.1016/j.jtice.2020.08.014
Innovation in membrane fabrication: Magnetic induced photocatalytic membrane
K. A. M. Said (2020)
10.1007/978-981-13-6549-2_7
Engineering of Micro/Nano Biosystems - Magnetophoresis in Bio-Devices
Anne-laure Deman (2019)
10.1063/1.4998604
Advances in microfluidic devices made from thermoplastics used in cell biology and analyses.
Elif Gencturk (2017)
10.1016/J.MEE.2019.01.005
Microfluidics with new multi-stage arc-unit structures for size-based cross-flow separation of microparticles
Yee-Ting Lee (2019)
10.1016/j.colsurfb.2017.06.008
Effective cell trapping using PDMS microspheres in an acoustofluidic chip.
D. Yin (2017)
10.1007/s10439-020-02593-y
Scalable Signature-Based Molecular Diagnostics Through On-chip Biomarker Profiling Coupled with Machine Learning
John H. Molinski (2020)
10.1088/1361-6528/ab4241
Magnetic nanoparticles in nanomedicine: a review of recent advances.
K. Wu (2019)
Semantic Scholar Logo Some data provided by SemanticScholar