Online citations, reference lists, and bibliographies.
← Back to Search

Chitosan Based Oligoamine Polymers: Synthesis, Characterization, And Gene Delivery.

B. Lu, C. Wang, De-Qun Wu, C. Li, X. Zhang, R. Zhuo
Published 2009 · Chemistry, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
A series of chitosan-based oligoamine polymers was synthesized from N-maleated chitosan (NMC) via Michael addition with diethylenetriamine (DETA), triethylenetetramine (TETA), tetraethylenepentamine (TEPA) and linear polyethylenimine (M(n) 423), respectively. The resulted polymers exhibited well binding ability to condense plasmid DNA to form complexes with size ranging from 200 to 600 nm when the polymer/DNA weight ratio was above 7. The polymer/DNA complexes observed by scanning electron microscopy (SEM) exhibited a compact and spherical morphology. The cytotoxicity assay showed that the synthesized polymers were less toxic than that of PEI(25 K). The gene transfection effect of resulted polymers was evaluated in 293T and HeLa cells, and the results showed that the gene transfection efficiency of these polymers was better than that of chitosan. Moreover, the transfection efficiency was dependent on the length of the oligoamine side chains and the molecular weight of the chitosan derivatives.
This paper references
10.1023/B:PHAM.0000026437.32238.6f
A Method for Oral DNA Delivery with N-Acetylated Chitosan
E. Kai (2004)
10.1146/ANNUREV.GENOM.2.1.177
Gene therapy: promises and problems.
A. Pfeifer (2001)
10.1016/j.biomaterials.2007.07.043
Poly(aspartate-g-PEI800), a polyethylenimine analogue of low toxicity and high transfection efficiency for gene delivery.
May P Xiong (2007)
10.1016/J.JCONREL.2007.10.013
Gene delivery using chitosan, trimethyl chitosan or polyethylenglycol-graft-trimethyl chitosan block copolymers: establishment of structure-activity relationships in vitro.
Oliver Germershaus (2008)
10.1021/BC0501597
PEI-g-chitosan, a novel gene delivery system with transfection efficiency comparable to polyethylenimine in vitro and after liver administration in vivo.
Kokhou Wong (2006)
The proton sponge: a trick to enter cells the viruses did not exploit
J. P. Behr (1997)
10.1016/J.BIOMATERIALS.2007.07.043
Poly(aspartate-g-PEI800), a polyethylenimine analogue of low toxicity and high transfection efficiency for gene delivery.
M. Xiong (2007)
10.1038/43977
Virus treatment questioned after gene therapy death
S. Lehrman (1999)
10.1016/J.IJPHARM.2007.07.011
Evaluation of chitosan salts as non-viral gene vectors in CHO-K1 cells.
Wanlop Weecharangsan (2008)
10.1021/bm8004676
Low molecular weight polyethylenimine grafted N-maleated chitosan for gene delivery: properties and in vitro transfection studies.
B. Lu (2008)
10.1016/J.BIOLOGICALS.2006.08.004
Optimization of 25 kDa linear polyethylenimine for efficient gene delivery.
Sun-Hyung Huh (2007)
10.1002/JBM.A.31520
A low-toxic and efficient gene vector: carboxymethyl dextran-graft-polyethylenimine.
Yun-xia Sun (2008)
10.1016/S0168-3659(97)00173-9
Preparation of chitosan self-aggregates as a gene delivery system.
K. Lee (1998)
10.1016/0168-3659(95)00007-U
In vitro cytotoxicity of macromolecules in different cell culture systems
Suthummar Choksakulnimitr (1995)
10.1016/J.JCONREL.2004.11.008
Polyethylenimine with acid-labile linkages as a biodegradable gene carrier.
Y. H. Kim (2005)
10.1016/S0142-9612(00)00385-9
In vitro gene delivery mediated by chitosan. effect of pH, serum, and molecular mass of chitosan on the transfection efficiency.
T. Sato (2001)
10.1038/sj.gt.3301110
Nonviral gene therapy: promises and challenges
S. Li (2000)
10.1038/32801
A triploblast origin for Myxozoa?
C. L. Anderson (1998)
10.1016/S0168-3659(00)00361-8
Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization and transfection efficiency.
H. Mao (2001)
10.1016/J.JCONREL.2005.02.004
Cationic microparticles consisting of poly(lactide-co-glycolide) and polyethylenimine as carriers systems for parental DNA vaccination.
C. Oster (2005)
10.1016/J.BIOMATERIALS.2004.01.051
Chondrogenic differentiation of human mesenchymal stem cells using a thermosensitive poly(N-isopropylacrylamide) and water-soluble chitosan copolymer.
J. Cho (2004)
PEI-g-chitosan
K. Wong (2006)
10.1016/S0168-3659(01)00513-2
Optimization of factors influencing the transfection efficiency of folate-PEG-folate-graft-polyethylenimine.
J. M. Benns (2002)
10.1016/S0168-3659(99)00090-5
Poly(ethylenimine) and its role in gene delivery.
W. Godbey (1999)
10.1126/SCIENCE.256.5058.808
Human gene therapy.
W. Anderson (1992)
10.1016/J.JCONREL.2005.05.004
Transfection efficiency of chitosan vectors: effect of polymer molecular weight and degree of deacetylation.
M. Huang (2005)
10.1166/JNN.2006.465
Folate receptor-mediated cancer cell specific gene delivery using folic acid-conjugated oligochitosans.
D. Lee (2006)
10.1016/J.JCONREL.2006.12.009
Biodistribution and tissue expression kinetics of plasmid DNA complexed with polyethylenimines of different molecular weight and structure.
Gil-Jae Jeong (2007)
10.1002/jgm.327
Relationship between the physical shape and the efficiency of oligomeric chitosan as a gene delivery system in vitro and in vivo
M. Köping-Höggård (2003)
10.1002/jgm.696
Exploring polyethylenimine‐mediated DNA transfection and the proton sponge hypothesis
A. Akinc (2005)
10.1021/BC020051G
N-alkylated chitosan as a potential nonviral vector for gene transfection.
W. G. Liu (2003)
10.1016/J.JCONREL.2003.08.017
Efficient gene delivery by urocanic acid-modified chitosan.
T. Kim (2003)
10.1038/7385
Oral gene delivery with chitosan–DNA nanoparticles generates immunologic protection in a murine model of peanut allergy
K. Roy (1999)
10.1021/BC0500701
A thermoresponsive chitosan-NIPAAm/vinyl laurate copolymer vector for gene transfection.
Shujun Sun (2005)
10.1021/BC049755B
Bioactive thin film of acidic fibroblast growth factor fabricated by layer-by-layer assembly.
Z. Mao (2005)
10.1016/S0168-3659(03)00076-2
Low-molecular-weight polyethylenimine as a non-viral vector for DNA delivery: comparison of physicochemical properties, transfection efficiency and in vivo distribution with high-molecular-weight polyethylenimine.
K. Kunath (2003)
10.1016/0003-2697(85)90442-7
Measurement of protein using bicinchoninic acid.
P. Smith (1985)
10.1016/S0142-9612(02)00445-3
In vitro cytotoxicity testing of polycations: influence of polymer structure on cell viability and hemolysis.
D. Fischer (2003)
Lowmolecular-weight polyethylenimine as a non-viral vector for DNA delivery: comparison of physicochemical properties
K. Kunath (2003)
10.1021/JA0436446
Hydroxyl stereochemistry and amine number within poly(glycoamidoamine)s affect intracellular DNA delivery.
Youfang Liu (2005)
10.1016/J.JCONREL.2005.09.040
Deoxycholic acid-conjugated chitosan oligosaccharide nanoparticles for efficient gene carrier.
S. Chae (2005)
10.1016/J.JCONREL.2005.03.011
Galactosylated polyethylenimine-graft-poly(vinyl pyrrolidone) as a hepatocyte-targeting gene carrier.
S. E. Cook (2005)
10.1002/ADMA.200600812
Cationic supramolecules composed of multiple oligoethylenimine-grafted β-cyclodextrins threaded on a polymer chain for efficient gene delivery
J. Li (2006)
10.1021/BC0000177
pH-sensitive cationic polymer gene delivery vehicle: N-Ac-poly(L-histidine)-graft-poly(L-lysine) comb shaped polymer.
J. M. Benns (2000)
In vitro gene deliverymediated by chitosan . Effect of pH , serum , andmolecular mass of chitosan on the transfection ef fi ciency
K. Roy (2001)
10.1038/38410
Gene therapy - promises, problems and prospects
I. Verma (1997)
10.1038/sj.gt.3302312
Improved chitosan-mediated gene delivery based on easily dissociated chitosan polyplexes of highly defined chitosan oligomers
M. Köping-Höggård (2004)
10.1002/jgm.773
Recent advances in rational gene transfer vector design based on poly(ethylene imine) and its derivatives
M. Neu (2005)
10.1016/J.BIOMATERIALS.2005.09.020
Characterization of folate-chitosan-DNA nanoparticles for gene therapy.
Sania Mansouri (2006)
10.1038/38653
Killing and culture
Henrietta S. Wilson (1997)
X
W. G. Liu (2003)
10.1016/j.jconrel.2007.10.013
Gene delivery using chitosan, trimethyl chitosan or polyethylenglycol-graft-trimethyl chitosan block copolymers: establishment of structure-activity relationships in vitro.
Oliver Germershaus (2008)
10.1021/BM034502R
Structural analysis of chitosan mediated DNA condensation by AFM: influence of chitosan molecular parameters.
S. Danielsen (2004)
10.1016/S0168-3659(00)00298-4
Galactosylated chitosan-graft-dextran as hepatocyte-targeting DNA carrier.
I. Park (2000)



This paper is referenced by
10.3390/ijms161226094
Therapeutic Potential of Cell Penetrating Peptides (CPPs) and Cationic Polymers for Chronic Hepatitis B
Bénédicte Ndéboko (2015)
10.1016/j.ijbiomac.2012.06.038
Preparation of low molecular weight N-maleated chitosan-graft-PAMAM copolymer for enhanced DNA complexation.
K. Sarkar (2012)
10.1016/j.ijpharm.2017.04.059
Limiting the level of tertiary amines on polyamines leads to biocompatible nucleic acid vectors.
Margarida Isabel Simão Carlos (2017)
10.1016/j.carbpol.2013.06.006
PAMAM conjugated chitosan through naphthalimide moiety for enhanced gene transfection efficiency.
K. Sarkar (2013)
10.3390/md15100298
Effect of Protonation State and N-Acetylation of Chitosan on Its Interaction with Xanthan Gum: A Molecular Dynamics Simulation Study
Suha M. Dadou (2017)
10.1016/j.biomaterials.2013.09.006
A pH-sensitive gene delivery system based on folic acid-PEG-chitosan - PAMAM-plasmid DNA complexes for cancer cell targeting.
M. Wang (2013)
10.1201/B15636-25
Chitosans as Delivery Vectors in Biomedical Applications
Vandana Bharat Patravale (2013)
10.1016/j.jconrel.2015.04.014
APC targeted micelle for enhanced intradermal delivery of hepatitis B DNA vaccine.
Buddhadev Layek (2015)
10.1016/j.ijpharm.2010.01.037
Folate mediated histidine derivative of quaternised chitosan as a gene delivery vector.
V. B. Morris (2010)
10.1179/1753555714Y.0000000202
Intercalative nanohybrid of DNA in laminated magnetic hydroxyapatite
G. F. Zuo (2015)
10.1039/C4RA13785J
Effect of monomer sequence of poly(histidine/lysine) catiomers on gene packing capacity and delivery efficiency
J. Zhou (2015)
10.1016/j.jconrel.2010.07.109
Effects of charge density and hydrophobicity of poly(amido amine)s for non-viral gene delivery.
M. Piest (2010)
10.1016/j.ijpharm.2014.12.062
Delivery of an adenovirus vector plasmid by ultrapure oligochitosan based polyplexes.
M. Agirre (2015)
10.1016/j.msec.2016.09.024
In vitro and in vivo assessment of chitosan modified urocanic acid as gene carrier.
Yu-Sheng Hsueh (2017)
10.1039/C8TB02414F
Zn(ii) coordination to cyclen-based polycations for enhanced gene delivery.
Q. Yu (2019)
10.1007/s10856-011-4494-1
Design and synthesis of dual-ligand modified chitosan as a liver targeting vector
Houxiang Chen (2011)
10.1016/B978-0-08-100228-5.00008-0
Chitosan for DNA and gene therapy
Buddhadev Layek (2017)
10.13028/M2W02Z
Disparate Activation of the Inflammasome by Chitin and Chitosan: A Dissertation
Chelsea L. Bueter (2013)
10.1039/c6ob00859c
Bio-reducible polycations from ring-opening polymerization as potential gene delivery vehicles.
Q. Yu (2016)
10.1016/j.carres.2010.12.019
Synthesis and characterization of carboxymethyl-polyaminate chitosan and its adsorption behavior toward a reactive dye.
Yuexia Gu (2011)
10.1002/mabi.201400518
Chitosan Grafted with Phosphorylcholine and Macrocyclic Polyamine as an Effective Gene Delivery Vector: Preparation, Characterization and In Vitro Transfection.
L. Li (2015)
10.1016/j.carbpol.2013.06.035
Blood compatible N-maleyl chitosan-graft-PAMAM copolymer for enhanced gene transfection.
K. Sarkar (2013)
10.1002/PI.3047
Folic acid‐conjugated depolymerized quaternized chitosan as potential targeted gene delivery vector
V. B. Morris (2011)
10.1002/ADFM.201202503
Components Simulation of Viral Envelope via Amino Acid Modified Chitosans for Efficient Nucleic Acid Delivery: In Vitro and In Vivo Study
Jing Chang (2013)
Nucleic acid delivery : biocompatible yet efficient platforms
M. I. Carlos (2015)
10.1021/ACS.BIOCONJCHEM.6B00251
Water-Based Chitosan for Thymine Conjugation: A Simple, Efficient, Effective, and Green Pathway to Introduce Cell Compatible Nucleic Acid Recognition.
Juthathip Fangkangwanwong (2016)
10.1007/s11595-015-1241-3
Synthesis and evaluation of novel chitosan derivatives for gene delivery
F. Fu (2015)
10.1039/C5TB02548F
"Stealthy" chitosan/mesoporous silica nanoparticle based complex system for tumor-triggered intracellular drug release.
M. Zhang (2016)
10.2147/DDDT.S77105
A study on the hemocompatibility of dendronized chitosan derivatives in red blood cells
Yanfang Zhou (2015)
10.1021/mp400269z
Methotrexate-loaded PEGylated chitosan nanoparticles: synthesis, characterization, and in vitro and in vivo antitumoral activity.
J. Chen (2014)
10.3109/10717544.2013.871373
Improving transfection efficiency of ultrapure oligochitosan/DNA polyplexes by medium acidification
M. Agirre (2015)
10.1016/j.carbpol.2017.05.099
Spermine modified starch-based carrier for gene delivery: Structure-transfection activity relationships.
X. Huang (2017)
See more
Semantic Scholar Logo Some data provided by SemanticScholar