Nanocomplexes Of Carboxymethyl Chitosan/amorphous Calcium Phosphate Reduce Oral Bacteria Adherence And Biofilm Formation On Human Enamel Surface.
Jiankang He, Yijun Bao, Jie Li, Zhongjun Qiu, Ying Liu, Xu Zhang
Published 2019 · Materials Science, Medicine
Download PDFAnalyze on Scholarcy
OBJECTIVES This study investigated the effect of CMC/ACP on oral bacteria adherence and biofilm formation on the enamel surface as well as the underlying mechanism to determine the anti-cariogenic potential of CMC/ACP. METHODS A mineral solution of CMC/ACP was characterised by transmission electron microscope. The bactericidal activity of CMC/ACP was evaluated with the plate count method. An in vitro biofilm model was established on saliva-coated enamel blocks; the effect of CMC/ACP on the adherence of Streptococcus mutans and Streptococcus gordonii to and biofilm formation on these blocks, as well as co-aggregation of Fusobacterium nucleatum was assessed by scanning electron microscopy, crystal violet staining, and confocal microscopy. Bacterial surface charge was estimated with the cytochrome c binding assay and by zeta potential measurement. RESULTS CMC/ACP nanocomplexes inhibited S. mutans and S. gordonii adherence to enamel blocks by 90% and 86% (P < 0.01), respectively, and biofilm formation by 45% and 44% (P < 0.01), respectively, after 24 h without bactericidal activity. CMC/ACP reduced F. nucleatum attachment to streptococcal biofilm by 75% (P < 0.01) while also altering cytochrome c binding to bacteria and reducing the zeta potential of the bacterial suspension. CONCLUSIONS CMC/ACP nanocomplexes inhibit cariogenic bacterial adherence, co-adhesion, and biofilm formation on the enamel surface, possibly by altering bacterial surface charge and enhancing the flocculation effect. As an agent that promotes remineralisation and has anti-cariogenic effects, CMC/ACP can be used to prevent and treat early caries and white spot lesions.
This paper references
Proteomics of Porphyromonas gingivalis within a model oral microbial community
Masae Kuboniwa (2009)
Interbacterial Adhesion Networks within Early Oral Biofilms of Single Human Hosts
Robert J. Palmer (2017)
The in vivo Inhibition of Oral Biofilm Accumulation and Streptococcus mutans by Ceramic Water
Ryota Nomura (2016)
Differential effect of autoinducer 2 of Fusobacterium nucleatum on oral streptococci.
Yun-Ji Jang (2013)
Interactions between chitosans and bacterial suspensions : adsorption and flocculation
Sabina P. Strand (2003)
Modifications in erythrocyte membrane zeta potential by Plasmodium falciparum infection.
F. Tokumasu (2012)
Functional Remineralization of Dentin Lesions Using Polymer-Induced Liquid-Precursor Process
Anora K. Burwell (2012)
Alteration of Zeta potential and membrane permeability in bacteria: a study with cationic agents
Suman Halder (2015)
Characteristics of Biofilm Formation by Streptococcus mutans in the Presence of Saliva
Sug-Joon Ahn (2008)
Antibacterial amorphous calcium phosphate nanocomposites with a quaternary ammonium dimethacrylate and silver nanoparticles.
Lei Cheng (2012)
Coaggregation-mediated interactions of streptococci and actinomyces detected in initial human dental plaque.
Robert J. Palmer (2003)
Preparation, characterization and antibacterial applications of carboxymethyl chitosan/CuO nanocomposite hydrogels.
Fazli Wahid (2017)
Is there a place for Tooth Mousse® in the prevention and treatment of early dental caries? A systematic review
Sarah L Raphael (2015)
Synergistic intrafibrillar/extrafibrillar mineralization of collagen scaffolds based on a biomimetic strategy to promote the regeneration of bone defects
Yao Wang (2016)
Influence of the Staphylococcus Aureus Bacteria Cells on the Zeta Potential of Graphene Oxide Modified with Alumina Nanoparticles in Electrolyte and Drinking Water Environment
Anna Jastrzębska (2015)
Bioinspired antimicrobial and biocompatible bacterial cellulose membranes obtained by surface functionalization with aminoalkyl groups.
Susana C M Fernandes (2013)
Effect of the electrostatic interaction on the redox reaction of positively charged cytochrome C adsorbed on the negatively charged surfaces of acid-terminated alkanethiol monolayers on a Au(111) electrode.
Shin-ichiro Imabayashi (2005)
Enhancing the stability and antibiofilm activity of DspB by immobilization on carboxymethyl chitosan nanoparticles.
Y. Tan (2015)
Bacterial diversity in the oral cavity of 10 healthy individuals
Elisabeth M. Bik (2010)
Rapid biomimetic remineralization of the demineralized enamel surface using nano-particles of amorphous calcium phosphate guided by chimaeric peptides.
Zuohui Xiao (2017)
Mutualistic biofilm communities develop with Porphyromonas gingivalis and initial, early, and late colonizers of enamel.
Saravanan Periasamy (2009)
Evaluation of the Proportion of Cariogenic Bacteria Associated with Dental Caries
Hiroya Gotouda (2017)
Stick to Your Gums
Angela H. Nobbs (2011)
Streptococcus mutans-derived extracellular matrix in cariogenic oral biofilms
Marlise Inêz Klein (2015)
Pyrosequencing analysis of the Oral Microflora of healthy adults
Bart J F Keijser (2008)
Autoinducer 2 of Fusobacterium nucleatum as a target molecule to inhibit biofilm formation of periodontopathogens.
Yun-Ji Jang (2013)
Prevention and Treatment of White Spot Lesions in Orthodontic Patients
Maryam Khoroushi (2017)
Determination of the electrokinetic potential of Rhizobium leguminosarum bv trifolii Rt24.2 using Laser Doppler Velocimetry--a methodological study.
Jolanta Ciesla (2011)
Critical roles of arginine in growth and biofilm development by Streptococcus gordonii.
Nicholas S Jakubovics (2015)
Recent concepts in plaque formation.
J-P Bernimoulin (2003)
Calcium carbonate nucleation driven by ion binding in a biomimetic matrix revealed by in situ electron microscopy.
Paul J. M. Smeets (2015)
Caries management by risk assessment: A review on current strategies for caries prevention and management
Shanmugavel Uma Maheswari (2015)
New Approaches to Enhanced Remineralization of Tooth Enamel
Nathan J Cochrane (2010)
Electroflocculation: the effect of zeta-potential on particle size
E. Ofir (2007)
Minimally Invasive Dentistry - A Review
Kumar Raghav Gujjar (2013)
Peptide-modified nanoparticles inhibit formation of Porphyromonas gingivalis biofilms with Streptococcus gordonii
Paridhi Kalia (2017)
Arginine-Ornithine Antiporter ArcD Controls Arginine Metabolism and Interspecies Biofilm Development of Streptococcus gordonii*♦
Akito Sakanaka (2015)
Flocculation, adhesion and biofilm formation in yeasts.
K. Verstrepen (2006)
THERAPIES FOR WHITE SPOT LESIONS—A SYSTEMATIC REVIEW
Anabela Baptista Paula (2017)
Effect of application sequence of fluoride and CPP-ACP on remineralization of white spot lesions in primary teeth: An in-vitro study.
Ola B. Al-Batayneh (2017)
Dental calculus: recent insights into occurrence, formation, prevention, removal and oral health effects of supragingival and subgingival deposits.
Donald James White (1997)
Oral multispecies biofilm development and the key role of cell–cell distance
Paul E. Kolenbrander (2010)
Anti-biofilm Activities from Bergenia crassifolia Leaves against Streptococcus mutans
Yucui Liu (2017)
MPC-Polymer Reduces Adherence and Biofilm Formation by Oral Bacteria
Kazuaki Hirota (2011)
Controlled adsorption of cytochrome c to nanostructured gold surfaces
Inês Gomes (2012)
Plausible Drug Targets in the Streptococcus mutans Quorum Sensing Pathways to Combat Dental Biofilms and Associated Risks
Gurmeet Kaur (2015)
Effects of Buffering Properties and Undissociated Acid Concentration on Dissolution of Dental Enamel in Relation to pH and Acid Type
R. Shellis (2013)
Effects of Material Properties on Bacterial Adhesion and Biofilm Formation
F. Song (2015)
The chemistry of caries: remineralization and demineralization events with direct clinical relevance.
Carlos González-Cabezas (2010)
The Fusobacterium nucleatum outer membrane protein RadD is an arginine-inhibitable adhesin required for inter-species adherence and the structured architecture of multispecies biofilm.
Christopher W. Kaplan (2009)
Strain-specific colonization patterns and serum modulation of multi-species oral biofilm development.
Basak Biyikoglu (2012)
Novel multifunctional dental cement to prevent enamel demineralization near orthodontic brackets.
Yansong Ma (2017)
Prevalence of white spot lesion formation during orthodontic treatment.
Katie C. Julien (2013)
Carboxymethyl chitosan prevents formation of broad-spectrum biofilm
Yulong Tan (2011)
Experimental evidence regarding the influence of surface charge on the bridging flocculation mechanism
Artur Schmitt (1997)
Understanding the chemistry of dental erosion.
John D. B. Featherstone (2006)
Biomimetic Remineralization of Demineralized Dentine Using Scaffold of CMC/ACP Nanocomplexes in an In Vitro Tooth Model of Deep Caries
Z. Chen (2015)
This paper is referenced by
Chitin and Chitosans: Characteristics, Eco-Friendly Processes, and Applications in Cosmetic Science
Cristina Casadidio (2019)
1 Chitin and Chitosans : Characteristics , Eco-Friendly 2 Processes and Applications in Cosmetic Science 3
Cristina Casadidio (2019)
Decrecimiento de Streptococcus mutans después de la aplicación de sellantes en superficies oclusales de molares permanentes en adultos
Patricio Vildósola Grez (2019)
Nanoparticles for the Treatment of Oral Biofilms: Current State, Mechanisms, Influencing Factors, and Prospects.
Chen Hu (2019)
Anti-biofilm and anti-metabolic effects of antimicrobial photodynamic therapy using chlorophyllin-phycocyanin mixture against Streptococcus mutans in experimental biofilm caries model on enamel slabs.
Shima Afrasiabi (2019)