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Characterization Of The Medicago Truncatula Cell Wall Proteome In Cell Suspension Culture Upon Elicitation And Suppression Of Plant Defense.

G. Gokulakannan, K. Niehaus
Published 2010 · Medicine, Biology

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In addition to establishing methods for proteome analysis of cell wall proteins (CWPs) for the model plant Medicago truncatula, this work highlights the presence of several protein classes in cell culture. Using a combination of two-dimensional gel electrophoresis (2D-PAGE) and/or liquid chromatography-tandem mass spectrometry (LC-MS/MS), we established the proteome reference map of M. truncatula cell wall proteins. CWPs extracted from purified cell wall fragments resulted in the identification of 46 (2D-PAGE) and 65 (LC-MS/MS) proteins, respectively, with a total of 111 proteins. The identified proteins are involved in various processes, including cell wall modifications, signaling, defense mechanisms, membrane transport, protein synthesis and processing. Further, we conducted comparative proteome analysis to identify changes in protein composition during interaction of M. truncatula cell suspension culture with a pathogen-derived yeast elicitor (YE) and suppressor using Sinorhizobium meliloti LPS. 2D-PAGE analysis for the CWPs after YE and LPS treatment resembled the proteome map of YE alone, with a few up-regulated proteins involved in defense, and in the case of the LPS-treated cell wall proteome, there was no significant difference observed. Using this approach, proteins involved in defense, such as l-ascorbate peroxidase, specifically targeted proteins to the cell wall during defense, including glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and proteins that play an important role during growth and development were identified. Also, some defense-related proteins were absent in the same gel after YE treatment, suggesting that oxidant protection is regulated by these proteins.
This paper references
10.1016/J.PHYTOCHEM.2004.04.026
Proteomics of Medicago sativa cell walls.
B. Watson (2004)
10.1105/tpc.010611
14-3-3 Proteins Are Constituents of the Insoluble Glycoprotein Framework of the Chlamydomonas Cell Wall Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.010611.
J. Voigt (2003)
10.1016/j.jplph.2008.09.010
Proteomics of weakly bound cell wall proteins in rice calli.
Xiong-Yan Chen (2009)
10.1016/j.tplants.2005.11.006
Cell wall proteins: a new insight through proteomics.
Elisabeth Jamet (2006)
10.1104/pp.107.098079
Functional Characterization of the Arabidopsis Eukaryotic Translation Initiation Factor 5A-2 That Plays a Crucial Role in Plant Growth and Development by Regulating Cell Division, Cell Growth, and Cell Death1[OA]
H. Feng (2007)
10.1104/PP.90.1.185
Soluble and Bound Apoplastic Activity for Peroxidase, beta-d-Glucosidase, Malate Dehydrogenase, and Nonspecific Arylesterase, in Barley (Hordeum vulgare L.) and Oat (Avena sativa L.) Primary Leaves.
Z. C. Li (1989)
10.1186/1746-4811-2-10
Evaluation of cell wall preparations for proteomics: a new procedure for purifying cell walls from Arabidopsis hypocotyls
Leila Feiz (2006)
10.1002/1522-2683(200206)23:11<1754::AID-ELPS1754>3.0.CO;2-E
Proteomic analysis of the Arabidopsis thaliana cell wall
S. Chivasa (2002)
10.1186/1471-2229-8-132
Elicitor-induced transcription factors for metabolic reprogramming of secondary metabolism in Medicago truncatula
Marina A. Naoumkina (2008)
10.1021/JF990916T
Changes in phytochemical and antioxidant activity of selected pepper cultivars (Capsicum species) as influenced by maturity.
L. Howard (2000)
10.1111/J.1469-8137.1992.TB04227.X
Developmental biology of legume nodulation
A. Hirsch (1992)
10.1042/BA20020118
Elicitation of plants and microbial cell systems
R. Radman (2003)
10.1016/J.PLAPHY.2004.10.014
Digging deeper into the plant cell wall proteome.
Sang-Jik Lee (2004)
10.1002/PMIC.200500047
Pathogen elicitor‐induced changes in the maize extracellular matrix proteome
S. Chivasa (2005)
10.1111/J.1365-313X.1993.TB00007.X
Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth.
N. Carpita (1993)
10.1002/ELPS.200305608
Proteomics of loosely bound cell wall proteins of Arabidopsis thaliana cell suspension cultures: A critical analysis
G. Borderies (2003)
Bacterial lipopolysaccharides : extraction with phenol-water and further applications of the procedure
O. Westphal (1965)
10.1111/J.1469-8137.2004.01214.X
The Lipid A substructure of the Sinorhizobium meliloti lipopolysaccharides is sufficient to suppress the oxidative burst in host plants.
Heiko Scheidle (2005)
10.1111/J.1432-1033.1985.TB08878.X
Metabolic changes in elicitor-treated bean cells. Enzymic responses associated with rapid changes in cell wall components.
G. Bolwell (1985)
10.1016/S0014-5793(02)02759-X
Pretreatment with salicylic acid primes parsley cells for enhanced ion transport following elicitation
V. Katz (2002)
10.1099/00221287-143-2-321
3-phosphoglycerate kinase: a glycolytic enzyme protein present in the cell wall of Candida albicans.
H. Alloush (1997)
10.1016/S0960-9822(06)00148-5
Protein transport: The nonclassical ins and outs
A. Cleves (1997)
10.1074/jbc.272.25.15841
Differential Extraction and Protein Sequencing Reveals Major Differences in Patterns of Primary Cell Wall Proteins from Plants*
D. Robertson (1997)
10.1023/A:1006110223774
Signals controlling the expression of cytosolic ascorbate peroxidase during pathogen-induced programmed cell death in tobacco
R. Mittler (2004)
10.1002/JSS.400060413
Plants interact with microbial polysaccharides.
P. Albersheim (1977)
10.1146/ANNUREV.BI.59.070190.004301
Defense-related proteins in higher plants.
D. Bowles (1990)
10.1002/PMIC.200500046
Arabidopsis cell wall proteome defined using multidimensional protein identification technology
E. Bayer (2006)
10.1128/JB.173.17.5371-5384.1991
Rhizobium lipopolysaccharide modulates infection thread development in white clover root hairs.
F. Dazzo (1991)
10.1128/IAI.63.11.4506-4514.1995
Structure and regulation of the HSP90 gene from the pathogenic fungus Candida albicans.
R. Swoboda (1995)
10.1042/BST0320524
Proteomic analysis of the Arabidopsis cell wall reveals unexpected proteins with new cellular locations.
A. Slabas (2004)
10.1111/J.1399-3054.1962.TB08052.X
A revised medium for rapid growth and bio assays with tobacco tissue cultures
T. Murashige (1962)
10.1007/BF00403191
Lipopolysaccharides of Rhizobium
L. Zevenhuizen (2004)
10.1073/PNAS.0600912103
Eukaryotic control on bacterial cell cycle and differentiation in the Rhizobium-legume symbiosis.
P. Mergaert (2006)
10.1186/1471-2229-8-94
A new picture of cell wall protein dynamics in elongating cells of Arabidopsis thaliana: Confirmed actors and newcomers
M. Irshad (2008)
10.1126/science.187.4171.74
Specific Elicitors of Plant Phytoalexin Production: Detenninants of Race Specificity in Pathogens?
N. Keen (1975)
10.1093/mp/ssp059
Plant cell wall proteomics: mass spectrometry data, a trove for research on protein structure/function relationships.
C. Albenne (2009)
10.1002/0471684228.egp02005
The cell wall
N. Carpita (2000)
10.1007/s00425-005-1563-z
Genomics of plant cell wall biogenesis
W. Yong (2005)
10.1146/ANNUREV.PHYTO.45.062806.094325
Cell wall-associated mechanisms of disease resistance and susceptibility.
R. Hückelhoven (2007)
10.1038/324365a0
Plant chitinases are potent inhibitors of fungal growth
Angela Schlumbaum (1986)
10.1016/S1360-1385(99)01405-3
Cooperative disassembly of the cellulose-xyloglucan network of plant cell walls: parallels between cell expansion and fruit ripening.
Rose. (1999)
10.1021/pr800291z
Characterization of the secretome of suspension cultures of Medicago species reveals proteins important for defense and development.
Lucia Kusumawati (2008)
Detection of pathogenesis-related proteins-chitinase and β-1,3-glucanase in induced chickpea
R. Saikia (2005)
10.1016/j.bbapap.2009.09.023
Cell wall proteome of wheat roots under flooding stress using gel-based and LC MS/MS-based proteomics approaches.
Fan-Jiang Kong (2010)
10.1002/pmic.200700938
Recent advances in plant cell wall proteomics
Elisabeth Jamet (2008)
10.1016/J.PLAPHY.2006.08.001
Plant glycoside hydrolases involved in cell wall polysaccharide degradation.
Z. Minic (2006)
10.1016/S1369-5266(99)80053-3
Medicago truncatula--a model in the making!
D. Cook (1999)
10.1128/JB.178.15.4724-4726.1996
Members of the Hsp70 family of proteins in the cell wall of Saccharomyces cerevisiae.
J. López-Ribot (1996)
10.1007/s00425-007-0668-y
Physiological roles of plant glycoside hydrolases
Z. Minic (2007)
10.1111/J.1574-6968.1999.TB13734.X
Enolase is present in the cell wall of Saccharomyces cerevisiae.
S. Edwards (1999)
10.1099/13500872-142-8-2311
The plasmin-binding protein Plr of group A streptococci is identified as glyceraldehyde-3-phosphate dehydrogenase.
S. B. Winram (1996)
10.1111/J.1469-8137.2006.01724.X
Modulation of reactive oxygen species activities and H2O2 accumulation during compatible and incompatible tomato-root-knot nematode interactions.
M. T. Melillo (2006)
10.1002/PMIC.200600953
Proteomics of ionically bound and soluble extracellular proteins in Medicago truncatula leaves
N. Soares (2007)
10.1046/J.0028-646X.2001.00214.X
Suppression of an elicitor‐induced oxidative burst reaction in Medicago sativa cell cultures by Sinorhizobium meliloti lipopolysaccharides
U. Albus (2001)
10.1093/JXB/ERJ149
Proteomic analysis of differentially expressed proteins in fungal elicitor-treated Arabidopsis cell cultures.
S. Chivasa (2006)
10.1105/tpc.104.026971
Cytosolic Ascorbate Peroxidase 1 Is a Central Component of the Reactive Oxygen Gene Network of Arabidopsisw⃞
S. Davletova (2005)
10.1016/0092-8674(92)90530-P
Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: A novel, rapid defense response
D. Bradley (1992)
10.1104/pp.108.118869
Eukaryotic Translation Initiation Factor 5A Is Involved in Pathogen-Induced Cell Death and Development of Disease Symptoms in Arabidopsis1[OA]
Marianne T Hopkins (2008)
10.1104/pp.106.090985
The Lipopolysaccharide of Sinorhizobium meliloti Suppresses Defense-Associated Gene Expression in Cell Cultures of the Host Plant Medicago truncatula1[W][OA]
Verena Tellström (2007)
10.5860/choice.38-3895
Biochemistry & Molecular Biology of Plants
B. Buchanan (2002)
10.1016/1369-5266(88)80060-8
Development of the arbuscular mycorrhizal symbiosis.
M. Harrison (1998)
10.1105/tpc.6.3.393
A higher plant extracellular vitronectin-like adhesion protein is related to the translational elongation factor-1 alpha.
J. Zhu (1994)
10.1111/j.1399-3054.2008.01198.x
Proteomic analysis of the secretome of rice calli.
Won Kyong Cho (2009)
10.1111/J.1365-3040.1988.TB01363.X
Elicitors and defence gene activation
M. Templeton (1988)



This paper is referenced by
6 Plant Protein Analysis
A. Malcevschi (2012)
10.1002/pmic.201500257
Legume proteomics: Progress, prospects, and challenges
D. Rathi (2016)
10.1186/1556-276X-9-589
Differential protein expression of Caco-2 cells treated with selenium nanoparticles compared with sodium selenite and selenomethionine
Linglin Fu (2014)
10.1007/978-81-322-2172-2_8
Plant Proteomics: Technologies and Applications
Deepti Bhushan Gupta (2015)
10.1002/pmic.201100359
Proteomics and plant disease: Advances in combating a major threat to the global food supply
C. Rampitsch (2012)
10.1021/pr400827a
Protein secretome of moss plants (Physcomitrella patens) with emphasis on changes induced by a fungal elicitor.
M. Lehtonen (2014)
10.3389/fpls.2015.01116
Proteomics and Metabolomics: Two Emerging Areas for Legume Improvement
A. Ramalingam (2015)
NANO EXPRESS Open Access
J. Lorbeer (2014)
Abiotic Stress-related Studies in Plants: Journey Towards Proteomics Era
Neelam Soda (2010)
10.5772/30785
Plant Protein Analysis
A. Malcevschi (2012)
10.3390/proteomes4030025
Extraction and Characterization of Extracellular Proteins and Their Post-Translational Modifications from Arabidopsis thaliana Suspension Cell Cultures and Seedlings: A Critical Review
Mina Ghahremani (2016)
10.1007/s11738-016-2266-8
A comparative cell wall proteomic analysis of cucumber leaves under Sphaerotheca fuliginea stress
Xiangnan Meng (2016)
10.1007/s12010-015-1638-7
Isolation and Analysis of Cell Wall Proteome in Elsholtzia splendens Roots Using ITRAQ with LC–ESI–MS/MS
Tingting Liu (2015)
10.1016/j.jprot.2019.103506
Cell wall proteome analysis of banana fruit softening using iTRAQ technology.
L. Xiao (2019)
10.1016/j.jprot.2011.11.006
Proteomic analysis of the compatible interaction between Vitis vinifera and Plasmopara viticola.
A. Milli (2012)
10.3389/fpls.2018.00535
Improving the Yield and Nutritional Quality of Forage Crops
Nicola M. Capstaff (2018)
10.1186/1471-2164-14-38
Novel aspects of grapevine response to phytoplasma infection investigated by a proteomic and phospho-proteomic approach with data integration into functional networks
P. Margaria (2012)
10.1016/j.plaphy.2012.07.021
Heat treatment of peach fruit: modifications in the extracellular compartment and identification of novel extracellular proteins.
Claudia A Bustamante (2012)
10.1186/1746-4811-7-48
Comparative evaluation of extraction methods for apoplastic proteins from maize leaves
K. Witzel (2011)
10.3390/ijms19010084
Transcriptomic Profiling and Physiological Analysis of Haloxylon ammodendron in Response to Osmotic Stress
Hui-Juan Gao (2017)
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