Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Epigenetic And Hormonal Profile During Maturation Of Quercus Suber L. Somatic Embryos.

M. Pérez, M. Viejo, M. Lacuesta, P. Toorop, M. Cañal
Published 2015 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Somatic embryogenesis is a powerful alternative to conventional mass propagation of Quercus suber L. However, poor quality and incomplete maturation of somatic embryos restrict any application. Given that epigenetic and hormonal control govern many developmental stages, including maturation of zygotic embryos, global DNA methylation and abscisic acid (ABA) were analyzed during development and maturation of cork oak somatic embryos. Our results indicated that development of somatic embryos concurred with a decrease in 5-mdC. In contrast, endogenous ABA content showed a transient increase with a peak in immature E2 embryos denoting the onset of the maturation phase. A cold stratification phase was necessary for embryos to acquire germination ability, which coincided with a significant decrease in 5-mdC and ABA content. Immunohistochemical analyses showed that there was a specific spatial-temporal regulation during embryogenesis, particularly after the cold treatment. The acquisition of germination capacity concurred with a general low 5-mdC signal in the root meristem, while retention of the 5-mdC signal was mainly located in the shoot meristem and provascular tissues. Conversely, ABA immunolocalization was mainly located in the root and shoot apical meristems. Furthermore, a strong decrease in the ABA signal was observed in the root cap after the stratification treatment suggesting a role for the root cap during development of somatic embryos. These results suggest that, in addition to ABA, epigenetic control appears to play an important role for the correct maturation and subsequent germination of cork oak somatic embryos.
This paper references
10.1126/science.1190614
Epigenetic Reprogramming in Plant and Animal Development
S. Feng (2010)
10.1007/s00468-013-0876-y
Temporary immersion systems (RITA®) for the improvement of cork oak somatic embryogenic culture proliferation and somatic embryo production
M. Pérez (2013)
10.1007/S11434-005-1191-9
Detection of DNA methylation changes during seed germination in rapeseed (Brassica napus)
Guang-yuan Lu (2006)
10.1093/mp/ssp027
An epigenetic perspective on developmental regulation of seed genes.
H. Zhang (2009)
10.1007/s00468-009-0370-8
DNA demethylation and decrease on free polyamines is associated with the embryogenic capacity of Pinus nigra Arn. cell culture
C. Noceda (2009)
10.1007/s11627-000-0062-y
Somatic embryogenesis in oak (Quercus spp.)
E. Wilhelm (2000)
10.1016/j.jplph.2009.04.014
Epigenetic characterization of the vegetative and floral stages of azalea buds: dynamics of DNA methylation and histone H4 acetylation.
M. Meijón (2009)
10.15258/SST.2003.31.1.12
Morphological and physiological maturation of acorns of cork oak (Quercus suber L.)
Hachemi Merouani (2003)
10.1007/s11240-007-9262-z
Involvement of DNA methylation in tree development and micropropagation
L. Valledor (2007)
10.3923/JPS.2007.341.346
Effects of Stratification and Growth Regulators on Seed Germination and Seedling Growth of Quercus ilex L.
.. M.Ghasemi (2007)
10.1016/S0092-8674(00)80700-X
The Stem Cell Population of Arabidopsis Shoot Meristems Is Maintained by a Regulatory Loop between the CLAVATA and WUSCHEL Genes
H. Schoof (2000)
10.1016/j.jplph.2010.09.015
Rapid responses of C14 clone of Eucalyptus globulus to root drought stress: Time-course of hormonal and physiological signaling.
Víctor Granda (2011)
10.1007/s11240-008-9435-4
Synthetic seed production from encapsulated somatic embryos of cork oak (Quercus suber L.) and automated growth monitoring
B. Pintos (2008)
10.1007/s00344-005-0110-2
Role of Abscisic Acid in Seed Dormancy
A. Kermode (2005)
10.1111/j.1744-7909.2008.00727.x
Abscisic acid-mediated epigenetic processes in plant development and stress responses.
V. Chinnusamy (2008)
10.2307/2260407
Seeds: Physiology of Development and Germination
J. Bewley (1986)
10.1007/BF00380037
Radioimmunoassays for the differential and direct analysis of free and conjugated abscisic acid in plant extracts
E. Weiler (2004)
10.1007/978-1-61779-818-4_22
Epigenetics, the role of DNA methylation in tree development.
M. Viejo (2012)
10.1111/J.1399-3054.1992.TB05259.X
Plant regeneration through somatic embryogenesis in Quercus suber
M. Bueno (1992)
10.1111/j.1365-313X.2010.04367.x
ABA promotes quiescence of the quiescent centre and suppresses stem cell differentiation in the Arabidopsis primary root meristem.
Hanma Zhang (2010)
10.1016/S1369-5266(99)80009-0
Shoot meristem formation and maintenance.
M. Lenhard (1999)
10.1023/A:1022002304479
Transient decreases in methylation at 5′-CCGG-3′ sequences in potato (Solanum tuberosum L.) meristem DNA during progression of tubers through dormancy precede the resumption of sprout growth
R. D. Law (2004)
10.1051/forest/2009098
Oak somatic and gametic embryos maturation is affected by charcoal and specific aminoacids mixture
B. Pintos (2011)
10.1007/s11240-011-9923-9
The role of abscisic acid in plant tissue culture: a review of recent progress
M. K. Rai (2011)
10.1016/j.pbi.2008.12.006
Epigenetic regulation of stress responses in plants.
V. Chinnusamy (2009)
10.1093/JEXBOT/52.365.2265
Immunohistochemical study of DNA methylation dynamics during plant development.
J. Žlůvová (2001)
10.1093/mp/ssp022
Chromatin remodeling in stem cell maintenance in Arabidopsis thaliana.
W. Shen (2009)
10.1111/J.1399-3054.1962.TB08052.X
A revised medium for rapid growth and bio assays with tobacco tissue cultures
T. Murashige (1962)
10.1007/s10535-012-0086-2
Analysis of DNA methylation during the germination of wheat seeds
F. Meng (2012)
10.1007/s11103-004-3380-3
Root cap specific expression of an endo-β-1,4--glucanase (cellulase): a new marker to study root development in Arabidopsis
E. Campillo (2004)
10.1007/BF01275450
Relationship between endogenous auxin and cytokinin levels and morphogenic responses inActinidia deliciosa tissue cultures
M. L. Centeno (2005)
10.1023/A:1016369921067
Loss of DNA methylation affects somatic embryogenesis in Medicago truncatula
D. Santos (2004)
Somatic embryo induction and germination in Quercus suber L.
J. A. Manzanera (1993)
10.1111/J.1365-313X.2005.02337.X
Genome-wide profiling of stored mRNA in Arabidopsis thaliana seed germination: epigenetic and genetic regulation of transcription in seed.
Kazumi Nakabayashi (2005)
10.1093/treephys/tpt033
Immunolocalization of IAA and ABA in roots and needles of radiata pine (Pinus radiata) during drought and rewatering.
N. De Diego (2013)
10.1007/s11103-008-9393-6
Hormone interactions at the root apical meristem
E. Benková (2008)
10.1079/IVP2004557
Effect of abscisic acid and stratification on somatic embryo maturation and germination of holm oak (Quercus ilex L.)
P. V. Mauri (2007)
10.1007/s00299-013-1418-1
ABA signaling in stress-response and seed development
K. Nakashima (2013)
10.1093/JXB/ERH193
Lateral ABA transport in maize roots (Zea mays): visualization by immunolocalization.
Daniela Schraut (2004)
10.1016/0003-2697(64)90204-0
THE PROBLEM OF HALTING ENZYME ACTION WHEN EXTRACTING PLANT TISSUES.
R. Bieleski (1964)
10.1093/JEXBOT/51.346.929
Radial transport of abscisic acid conjugates in maize roots: its implication for long distance stress signals.
A. Sauter (2000)
10.1073/PNAS.95.10.5824
DNA methylation and the promotion of flowering by vernalization.
E. Finnegan (1998)
10.1016/S0168-9452(03)00127-4
Detection of DNA methylation changes during somatic embryogenesis of Siberian ginseng (Eleuterococcus senticosus)
D. Chakrabarty (2003)
10.1007/s00497-010-0145-9
DNA methylation during sexual embryogenesis and implications on the induction of somatic embryogenesis in Castanea sativa Miller
M. Viejo (2010)
10.1016/J.TPLANTS.2007.06.003
Combined networks regulating seed maturation.
L. Gutierrez (2007)
10.1007/s11240-004-1056-y
Effect of exogenous ABA on embryo maturation and quantification of endogenous levels of ABA and IAA in Quercus suber somatic embryos
G. García-Martín (2004)
Endogenous Factors that Regulate Plant Embryogenesis: Recent Advances
Mikihisa Umehara (2007)
10.1007/s00468-005-0012-8
Physiological and morphological characteristics during development of pedunculate oak (Quercus robur L.) zygotic embryos
Christine Prewein (2005)
10.5772/35960
Combinatorial Networks Regulating Seed Development and Seed Filling
M. Gao (2012)
10.1086/336802
Differentiation of Plantlets in Longleaf Pine (Pinus palustris Mill.) Tissue Cultured In vitro
H. E. Sommer (1975)
10.1016/j.plaphy.2008.04.009
HPCE quantification of 5-methyl-2'-deoxycytidine in genomic DNA: methodological optimization for chestnut and other woody species.
R. Hasbún (2008)
10.1016/j.jplph.2009.02.014
Acetylated H4 histone and genomic DNA methylation patterns during bud set and bud burst in Castanea sativa.
M. E. Santamaria (2009)
10.1007/s00299-012-1248-6
Direct and indirect organogenesis of Clivia miniata and assessment of DNA methylation changes in various regenerated plantlets
Q. Wang (2012)
10.1111/J.1365-313X.2007.03118.X
Seed dormancy release in Arabidopsis Cvi by dry after-ripening, low temperature, nitrate and light shows common quantitative patterns of gene expression directed by environmentally specific sensing.
W. Finch-Savage (2007)
10.1242/jcs.00406
Stem cell regulation in the shoot meristem
R. Gross-Hardt (2003)
10.1007/s10342-011-0526-0
Application of biotechnological tools to Quercus improvement
A. M. Vieitez (2011)



This paper is referenced by
10.1016/j.plantsci.2016.09.012
Glutathione and abscisic acid supplementation influences somatic embryo maturation and hormone endogenous levels during somatic embryogenesis in Podocarpus lambertii Klotzsch ex Endl.
H. Fraga (2016)
10.1021/acs.jafc.7b02255
Multi-imaging of Cytokinin and Abscisic Acid on the Roots of Rice (Oryza sativa) Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry.
Katsuhiro Shiono (2017)
10.3390/ijms21186849
Hypermethylation of Auxin-Responsive Motifs in the Promoters of the Transcription Factor Genes Accompanies the Somatic Embryogenesis Induction in Arabidopsis
D. Grzybkowska (2020)
10.1007/978-3-319-33705-0_6
Epigenetic Advances in Somatic Embryogenesis in Sequenced Genome Crops
Fátima Duarte-Aké (2016)
10.1007/s11738-017-2487-5
New insights into plant somatic embryogenesis: an epigenetic view
V. Kumar (2017)
10.1007/s11240-014-0706-y
Expression analysis of epigenetic and abscisic acid-related genes during maturation of Quercus suber somatic embryos
M. Pérez (2014)
Embriogénesis somática e inducción de callo in vitro en especies fabáceas nativas del estado de Nuevo León.
Alejandro Ibarra López (2019)
DNA methylation dynamics throughout indirect somatic embryogenesis of tamarillo
Matilde Lopes Telmo Calado Sanches (2019)
10.3389/fpls.2019.00240
Somatic Embryogenesis Induction in Woody Species: The Future After OMICs Data Assessment
M. S. Pais (2019)
10.3389/fpls.2018.01194
Cork Oak Young and Traumatic Periderms Show PCD Typical Chromatin Patterns but Different Chromatin-Modifying Genes Expression
Vera Inácio (2018)
10.1007/s00344-015-9510-0
The Effects of Hormone Treatment on Epigenetic Marks During Organogenesis in Pinus radiata D. Don Embryos
J. L. Rodríguez (2015)
Utilisation de la cryoconservation pour la conservation et la production de cultures in vitro de palmier dattier (Phoenix dactylifera L.) : Impact d'un protocole de cryoconservation sur la physiologie des cals embryogènes de palmier dattier
M. Salma (2015)
10.1093/treephys/tpv127
Integrated physiological and hormonal profile of heat-induced thermotolerance in Pinus radiata.
Mónica Escandón (2016)
10.3389/fpls.2015.00635
The role of chromatin modifications in somatic embryogenesis in plants
Clelia De-la-Peña (2015)
10.1007/s11295-019-1353-6
Transcriptome dynamics of cork oak (Quercus suber) somatic embryogenesis reveals active gene players in transcription regulation and phytohormone homeostasis of embryo development
Tiago Capote (2019)
10.3390/ijms19103182
5-Azacytidine: A Promoter of Epigenetic Changes in the Quest to Improve Plant Somatic Embryogenesis
Pedro Osorio-Montalvo (2018)
Efficient plant regeneration of Malaysian aromatic rice ( Oryza sativa L . ) through somatic embryogenesis
Z. A. Rahman (2015)
10.1007/s00468-016-1416-3
Cryopreservation changes the DNA methylation of embryonic axes of Quercus robur and Fagus sylvatica seeds during in vitro culture
K. Nuc (2016)
10.1590/1981-6723.07918
Phenolic compound, nutritional and antioxidant profile of pollen collected by the genus melipona in North Eastern Brazil
Rosane Gomes de Oliveira (2019)
10.1007/978-1-0716-0876-0_17
Immunochemical Detection of Modified Species of Cytosine in Plant Tissues.
Marcos Viejo (2021)
10.1016/B978-0-12-811562-6.00013-X
Phytohormonal quantification based on biological principles
Yi Su (2017)
Semantic Scholar Logo Some data provided by SemanticScholar