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A Simplified Mycobacterial Growth Inhibition Assay (MGIA) Using Direct Infection Of Mouse Splenocytes And The MGIT System.

A. Yang, T. E. Schmidt, S. Stibitz, S. Derrick, S. Morris, M. Parra
Published 2016 · Biology, Medicine

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We describe a simplified Mycobacterial Growth Inhibition Assay (MGIA) for pre-clinical assessment of vaccine-mediated protection in mice. The assay is accomplished by directly infecting splenocytes from vaccinated mice with Mycobacterium tuberculosis and quantifying mycobacteria using Mycobacterial Growth Indicator Tubes (MGIT). Vaccine-mediated immunogenicity detected by this assay correlated with protection.
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