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A Simplified Mycobacterial Growth Inhibition Assay (MGIA) Using Direct Infection Of Mouse Splenocytes And The MGIT System.
A. Yang, T. E. Schmidt, S. Stibitz, S. Derrick, S. Morris, M. Parra
Published 2016 · Biology, Medicine
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We describe a simplified Mycobacterial Growth Inhibition Assay (MGIA) for pre-clinical assessment of vaccine-mediated protection in mice. The assay is accomplished by directly infecting splenocytes from vaccinated mice with Mycobacterium tuberculosis and quantifying mycobacteria using Mycobacterial Growth Indicator Tubes (MGIT). Vaccine-mediated immunogenicity detected by this assay correlated with protection.
This paper references
Rapid Diagnosis of Tuberculosis and Multidrug Resistance Using a MGIT 960 System
Won-Jung Koh (2012)
In vitro measurement of protective mycobacterial immunity: antigen-specific expansion of T cells capable of inhibiting intracellular growth of bacille Calmette-Guérin.
S. Worku (2000)
Development of a Murine Mycobacterial Growth Inhibition Assay for Evaluating Vaccines against Mycobacterium tuberculosis
M. Parra (2009)
CD4+ T Cells Mediate IFN-γ-Independent Control of Mycobacterium tuberculosis Infection Both In Vitro and In Vivo
S. Cowley (2003)
Multicentre evaluation of an automated BACTEC 960 system for susceptibility testing of Mycobacterium tuberculosis.
C. M. S. Giampaglia (2007)
Vaccine-induced anti-tuberculosis protective immunity in mice correlates with the magnitude and quality of multifunctional CD4 T cells.
S. Derrick (2011)
Investigation of the relationships between immune-mediated inhibition of mycobacterial growth and other potential surrogate markers of protective Mycobacterium tuberculosis immunity.
D. Hoft (2002)
Effect of cationic liposomes on BCG trafficking and vaccine-induced immune responses following a subcutaneous immunization in mice.
S. Derrick (2015)
Time to Detection of Mycobacterium tuberculosis Using the MGIT 320 System Correlates with Colony Counting in Preclinical Testing of New Vaccines
K. Kolibab (2013)
Lymphocyte-dependent inhibition of growth of virulent Mycobacterium tuberculosis H37Rv within human monocytes: requirement for CD4+ T cells in purified protein derivative-positive, but not in purified protein derivative-negative subjects.
R. F. Silver (1998)
Time to detection of Mycobacterium tuberculosis as an alternative to quantitative cultures.
C. Bark (2011)
Comparison of the Mycobacteria Growth Indicator Tube (MGIT) with radiometric and solid culture for recovery of acid-fast bacilli.
G. Pfyffer (1997)
Efficacy of BCG Vaccine in the Prevention of Tuberculosis: Meta-analysis of the Published Literature
G. Colditz (1994)
Bactericidal Activity in Whole Blood as a Potential Surrogate Marker of Immunity after Vaccination against Tuberculosis
Seon-Hee Cheon (2002)
Evaluation of the BD Bactec MGIT 320 System for Detection of Mycobacteria and Drug Susceptibility Testing of Mycobacterium tuberculosis
Abigail Duque (2013)
Mycobacterial growth inhibition in murine splenocytes as a surrogate for protection against Mycobacterium tuberculosis (M. tb).
L. Marsay (2013)
Multicenter Laboratory Validation of the BACTEC MGIT 960 Technique for Testing Susceptibilities of Mycobacterium tuberculosis to Classical Second-Line Drugs and Newer Antimicrobials
S. Rüsch-Gerdes (2006)
BCG: The Challenge Continues
P. Fine (2001)
A practical in vitro growth inhibition assay for the evaluation of TB vaccines.
K. Kolibab (2009)
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