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Glutathione Content Is Altered In Müller Cells Of Monkey Eyes With Experimental Glaucoma
L. Carter-Dawson, F. Shen, R. Harwerth, M. L. Crawford, Amy Whitetree
Published 2004 · Biology, Medicine
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Extracellular levels of glutamate are thought to be increased in glaucoma and thus contribute to retinal damage. An increase in glutamate concentration or duration in the extracellular retinal space is expected to impact glutathione content in Müller cells since glutamate is the rate-limiting constituent in glutathione synthesis. We have investigated whether glutathione content is changed in retinal Müller cells of monkeys with experimental glaucoma using immunocytochemistry and image analysis. Müller cells in glaucomatous retinas showed significantly greater immunoreactivity (27-57%) for glutathione than those in fellow control retinas, increasing with the duration of elevated intraocular pressure (IOP). This pattern of labeling was prominent in all four monkeys studied. The presence of more glutathione in Müller cells of glaucomatous retinas is consistent with an increase in extracellular glutamate and an increase in transport and metabolism of glutamate.
This paper references
Role of two recently cloned rat liver GSH transporters in the ubiquitous transport of GSH in mammalian cells.
S. Lu (1996)
Protection from oxidant injury by sodium-dependent GSH uptake in retinal Müller cells.
R. Kannan (1999)
Neuroprotective Effect of Memantine in Different Retinal Injury Models in Rats
E. Woldemussie (2002)
Experimental Glaucoma: Perimetric Field Defects and Intraocular Pressure
R. Harwerth (1997)
Efficacy and safety of memantine, an NMDA-type open-channel blocker, for reduction of retinal injury associated with experimental glaucoma in rat and monkey.
W. Hare (2001)
Glutathione Pathways in the Brain
R. Dringen (2003)
The induction of GSH synthesis by nanomolar concentrations of NO in endothelial cells: a role for γ‐glutamylcysteine synthetase and γ‐glutamyl transpeptidase
D. Moellering (1999)
GSH transport in human cerebrovascular endothelial cells and human astrocytes: evidence for luminal localization of Na+-dependent GSH transport in HCEC
R. Kannan (2000)
Immunocytochemical evidence for the presence of high levels of reduced glutathione in radial glial cells and horizontal cells in the rabbit retina
D. Pow (1995)
Vitreous amino acid concentrations in patients with glaucoma undergoing vitrectomy.
R. Honkanen (2003)
Vitreal glutamate concentration in monkeys with experimental glaucoma.
L. Carter-Dawson (2002)
Glutamate receptors induce a burst of superoxide via activation of nitric oxide synthase in arginine-depleted neurons.
M. Culcasi (1994)
Elevated glutamate levels in the vitreous body of humans and monkeys with glaucoma.
E. Dreyer (1996)
Retinal TUNEL-positive cells and high glutamate levels in vitreous humor of mutant quail with a glaucoma-like disorder.
O. Dkhissi (1999)
The glutathione level of retinal Müller glial cells is dependent on the high-affinity sodium-dependent uptake of glutamate
W. Reichelt (1997)
Measurement of amino acid levels in the vitreous humor of rats after chronic intraocular pressure elevation or optic nerve transection.
H. Levkovitch-Verbin (2002)
Differential effect of nitric oxide on glutathione metabolism and mitochondrial function in astrocytes and neurones: implications for neuroprotection/neurodegeneration?
M. Gegg (2003)
Glutamine immunoreactivity in Müller cells of monkey eyes with experimental glaucoma.
L. Carter-Dawson (1998)
Vitreous body glutamate concentration in dogs with glaucoma.
D. E. Brooks (1997)
Retinal glutamate transporter changes in experimental glaucoma and after optic nerve transection in the rat.
Keith R G Martin (2002)
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Glutathione Metabolism and Its Contribution to Antiapoptotic Properties of α-Crystallins in the Retina
P. G. Sreekumar (2012)
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Mai Akane (2011)
Sodium Iodate Influences the Apoptosis, Proliferation and Differentiation Potential of Radial Glial Cells In Vitro
X. Chen (2014)
Circulating Total Glutathione in Normal Tension Glaucoma Patients: Comparison with Normal Control Subjects
M. Park (2012)
The Primate Model of Experimental Glaucoma
A. Weber (2008)
[Roles of retinal Müller cells in health and glaucoma].
F. Gao (2013)
An hypothesis to account for the renewal of outer segments in rod and cone photoreceptor cells: renewal as a surrogate antioxidant.
Barry S. Winkler (2008)
A Na+/Ca2+ exchanger isoform, NCX1, is involved in retinal cell death after N‐methyl‐D‐aspartate injection and ischemia–reperfusion
Y. Inokuchi (2009)
The role of Müller glia and microglia in glaucoma
Roswitha Seitz (2013)
Glutathione-Related Enzymes and the Eye
E. Ganea (2006)
Genetic and Biochemical Biomarkers in Canine Glaucoma
K. Graham (2017)
Progesterone-treatment in a model of Retinitis Pigmentosa: the rd1 mice
Violeta Sánchez Vallejo (2015)
alpha-Luminol prevents decreases in glutamate, glutathione, and glutamine synthetase in the retinas of glaucomatous DBA/2J mice.
J. R. Gionfriddo (2009)
Glaucoma and Helicobacter pylori infection: correlations and controversies
A. Izzotti (2009)
Phosphodiesterase Inhibitors Sildenafil and Vardenafil Reduce Zebrafish Rod Photoreceptor Outer Segment Shedding
L. J. Campbell (2017)
K+ currents fail to change in reactive retinal glial cells in a mouse model of glaucoma
Sylvia Bolz (2008)
Quantification of retinal transneuronal degeneration in human glaucoma: a novel multiphoton-DAPI approach.
Y. Lei (2008)
Autocrine and paracrine interactions and neuroprotection in glaucoma
A. Weber (2013)
Oxidative Stress in Glaucoma: A Burden of Evidence
Domalapalli Maneesh Kumar (2007)
Neuroprotective and Intraocular Pressure-Lowering Effects of (–)Δ9-Tetrahydrocannabinol in a Rat Model of Glaucoma
J. Crandall (2007)
Oxidative stress increases in retinas of dogs in acute glaucoma but not in chronic glaucoma.
Thomas W. Y. Chen (2015)
The use of Eudragit RS 100/cyclodextrin nanoparticles for the transmucosal administration of glutathione.
A. Lopedota (2009)
Friend or foe? Resolving the impact of glial responses in glaucoma.
E. Johnson (2009)
Experimental glutamatergic excitotoxicity in rabbit retinal ganglion cells: block by memantine.
W. Hare (2009)