Real-time Detection Of The Chemokine CXCL12 In Urine Samples By Surface Plasmon Resonance.
B. Vega, A. Calle, A. Sánchez, L. Lechuga, A. M. Ortiz, G. Armelles, J. M. Rodríguez-Frade, M. Mellado
Published 2013 · Chemistry, Medicine
Download PDFAnalyze on Scholarcy
Surface plasmon resonance (SPR)-based biosensors are established tools for measuring biomolecular interactions between unlabeled analytes in real time, and are thus an ideal method to evaluate G protein-coupled receptor (GPCR) binding interactions. Using as a vehicle lentiviral particles bearing the chemokine receptor CXCR4 in its native plasma membrane context, SPR analysis can be performed using the particles as specific receptors to monitor the CXCR4 interaction with its ligand, CXCL12. The method shows linear correlation in the 5-40 nM range, with low intra- and inter-assay variation, a relative standard deviation <10%, chip-to-chip variation <12%, with stability of the sensor response for more than 150 measurements in the same chip over a four-week period. Our objective was to develop a method for rapid detection and quantification of analytes such as CXCL12 in biological samples, with no need for pretreatment. As a proof of concept, we tested for CXCL12 in urine samples from rheumatoid arthritis patients, who have elevated levels of this chemokine in plasma and synovial fluid. The biosensor method allowed sensitive, reproducible CXCL12 detection in the physiological range, suggesting its value for the diagnosis of autoimmune disorders.
This paper references
Exploring biomolecular recognition using optical biosensors.
G. Canziani (1999)
The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry
C. Bleul (1996)
Technical advance: Surface plasmon resonance-based analysis of CXCL12 binding using immobilized lentiviral particles.
B. Vega (2011)
The bicyclam AMD3100 story
E. Clercq (2003)
Characterization of the Stromal Cell-derived Factor-1α-Heparin Complex*
R. Sadir (2001)
Matrix effects in immunobiosensor determination of clenbuterol in urine and serum.
M. Johansson (2004)
Inhibiting citrullination in rheumatoid arthritis: taking fuel from the fire
Benjamin A Fisher (2012)
Van Damme A.
M. Haine (1986)
Application of surface plasmon resonance toward studies of low-molecular-weight antigen-antibody binding interactions.
Maciej Adamczyk (2000)
Multiplexed electrical detection of cancer markers with nanowire sensor arrays
G. Zheng (2005)
Capture and reconstitution of G protein-coupled receptors on a biosensor surface.
Peter Stenlund (2003)
Covalent coupling of immunoglobulin G to self-assembled monolayers as a method for immobilizing the interfacial-recognition layer of a surface plasmon resonance immunosensor.
D. M. Disley (1998)
Weighing of biomolecules, single cells and single nanoparticles in fluid
T. Burg (2007)
Recombinant G protein-coupled receptors from expression to renaturation: a challenge towards structure
V. Sarramégna (2006)
Solubilization, stabilization, and purification of chemokine receptors using biosensor technology.
Iva Navrátilová (2005)
The Role of Post-translational Modifications of the CXCR4 Amino Terminus in Stromal-derived Factor 1α Association and HIV-1 Entry*
M. Farzan (2002)
How to validate analytical methods
R. Wood (1999)
Mapping the binding of the N-terminal extracellular tail of the CXCR4 receptor to stromal cell-derived factor-1alpha.
Elliott K Gozansky (2005)
Effect of posttranslational processing on the in vitro and in vivo activity of chemokines.
A. Mortier (2011)
Mutations at the CXCR4 interaction sites for AMD3100 influence anti‐CXCR4 antibody binding and HIV‐1 entry
S. Hatse (2003)
Relationships between glycosaminoglycan and receptor binding sites in chemokines-the CXCL12 example.
C. Laguri (2008)
CXCL12 chemokine up-regulates bone resorption and MMP-9 release by human osteoclasts: CXCL12 levels are increased in synovial and bone tissue of rheumatoid arthritis patients.
F. Grassi (2004)
Urinary glycosaminoglycans in patients with systemic lupus erythematosus.
P. De Muro (2001)
The chemokine receptor CXCR4 is essential for vascularization of the gastrointestinal tract
K. Tachibana (1998)
Plasma level of CXC-chemokine CXCL12 is increased in rheumatoid arthritis and is independent of disease activity and methotrexate treatment.
I. B. Hansen (2006)
The monomer-dimer equilibrium of stromal cell-derived factor-1 (CXCL 12) is altered by pH, phosphate, sulfate, and heparin.
Christopher T. Veldkamp (2005)
Chemokines and disease
C. Gerard (2001)
Citrullination of CXCL8 by peptidylarginine deiminase alters receptor usage, prevents proteolysis, and dampens tissue inflammation
P. Proost (2008)
Molecular-Level Control over Surface Order in Self-Assembled Monolayer Films of Thiols on Gold
C. Bain (1988)
Chemokines and chemokine receptors in rheumatoid arthritis.
Z. Szekanecz (2003)
Present and future of surface plasmon resonance biosensors
J. Homola (2003)
High-Level Production, Solubilization and Purification of Synthetic Human GPCR Chemokine Receptors CCR5, CCR3, CXCR4 and CX3CR1
Hui Ren (2009)
Stimulation of matrix metalloprotease 3 release from human chondrocytes by the interaction of stromal cell-derived factor 1 and CXC chemokine receptor 4.
K. Kanbe (2002)
CXCL12 (SDF-1)/CXCR4 Pathway in Cancer
B. Teicher (2010)
Amino acid composition of heparan sulfate and chondroitin sulfates in normal urine.
Naoyuki Taniguchi (1973)
Determination of carbaryl in natural water samples by a surface plasmon resonance flow-through immunosensor.
E. Mauriz (2006)
Defects of B-cell lymphopoiesis and bone-marrow myelopoiesis in mice lacking the CXC chemokine PBSF/SDF-1
T. Nagasawa (1996)
Stromal Cell-Derived Factor-1-CXC Chemokine Receptor 4 Interactions Play a Central Role in CD4+ T Cell Accumulation in Rheumatoid Arthritis Synovium1
T. Nanki (2000)
Biosensor diagnosis of urinary tract infections: a path to better treatment?
K. Mach (2011)
NMR analyses of the interaction between CCR5 and its ligand using functional reconstitution of CCR5 in lipid bilayers.
Chie Yoshiura (2010)
Monomeric and dimeric CXCL12 inhibit metastasis through distinct CXCR4 interactions and signaling pathways
Luke J. Drury (2011)
This paper is referenced by
Equilibrium-fluctuation analysis for interaction studies between natural ligands and single G protein-coupled receptors in native lipid vesicles.
Olov Wahlsten (2015)
Chemokine Detection Using Receptors Immobilized on an SPR Sensor Surface.
José Miguel Rodríguez-Frade (2016)
CysLT1 receptor antagonist alleviates pathogenesis of collagen-induced arthritis mouse model
Minwen Xu (2017)
Development of novel bioanalytical assays with single-molecule readout for biomarker detection and drug candidate characterization
O. Wahlsten (2017)
Biosensors and nanobiosensors for rapid detection of autoimmune diseases: a review
F. Ghorbani (2019)
Molecular targeted study in tumors: From western medicine to active ingredients of traditional Chinese medicine.
R. Li (2019)
Surface plasmon resonance: advances of label-free approaches in the analysis of biological samples.
Tomáš Riedel (2014)
G Protein-Coupled Receptor Screening Assays: Methods and Protocols
Duarte M F Prazeres (2015)
Nanoyeast and Other Cell Envelope Compositions for Protein Studies and Biosensor Applications
Yadveer S Grewal (2016)
Methods to immobilize GPCR on the surface of SPR sensors.
Laura Martı́nez-Muñoz (2015)
Characterisation of targeted imaging and theranostic agents for cancer and fibrosis
J. A. Thompson (2018)
The effects of lipids and surfactants on TLR5-proteoliposome functionality for flagellin detection using surface plasmon resonance biosensing.
Yusser Olguín (2014)
Immunosensors for Biomarker Detection in Autoimmune Diseases
Xuezhu Zhang (2016)
A compact SPR biosensor device for the rapid and efficient monitoring of gluten-free diet directly in human urine
E. C. Pelaez (2020)