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Electrochemiluminescence Biosensor For Determination Of Organophosphorous Pesticides Based On Bimetallic Pt-Au/multi-walled Carbon Nanotubes Modified Electrode.
S. Miao, M. S. Wu, L. Ma, X. He, Hong Yang
Published 2016 · Chemistry, Medicine
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A novel and highly sensitive electrochemiluminescence (ECL) biosensing system was designed and developed for individual detection of different organophosphorous pesticides (OPs) in food samples. Bimetallic Pt-Au nanoparticles were electrodeposited on multi-walled carbon nanotubes (MWNTs)-modified glass carbon electrode (GCE) to increase the surface area of electrode and ECL signals of luminol. Biocomposites of enzymes from acetylcholinesterase and choline oxidase (AChE and ChOx) were immobilized onto the electrode surface to produce massive hydrogen peroxides (H2O2), thus amplifying ECL signals. Based on the dual-amplification effects of nanoparticles and H2O2 produced by enzymatic reactions, the proposed biosensor exhibits highly sensitivity. The proposed biosensing approach was then used for detecting OPs by inhibition of OPs on AChE. Under optimized experimental conditions, the ECL intensity decreased accordingly with the increase in concentration of OPs, and the inhibition rates of OPs were proportional to their concentrations in the range of 0.1-50nmolL(-1) for malathion, methyl parathion and chlorpyrifos, with detection limit of 0.16nmolL(-1), 0.09nmolL(-1) and 0.08nmolL(-1), respectively. The linearity range of the biosensor for pesticide dufulin varied from 50 to 500nmolL(-1), with the detection limit of 29.7nmolL(-1). The resulting biosensor was further validated by assessment of OPs residues in cabbage, which showed a fine applicability for the detection of OPs in the realistic sample.
This paper references
Covalent coupling of organophosphorus hydrolase loaded quantum dots to carbon nanotube/Au nanocomposite for enhanced detection of methyl parathion.
D. Du (2010)
Direct electrodeposition of graphene enabling the one-step synthesis of graphene-metal nanocomposite films.
C. Liu (2011)
Synthesis, characterization, and electrochemiluminescence of luminol-reduced gold nanoparticles and their application in a hydrogen peroxide sensor.
Hua Cui (2007)
Electrochemiluminescent immunosensor based on CdS quantum dots for ultrasensitive detection of microcystin-LR
J. Zhang (2015)
Electrochemiluminescent biosensor based on multi-wall carbon nanotube/nano-Au modified electrode
Zhenyu Lin (2008)
Edge plane pyrolytic graphite electrodes in electroanalysis: an overview.
C. Banks (2005)
Multichannel electrochemiluminescence of luminol in neutral and alkaline aqueous solutions on a gold nanoparticle self-assembled electrode.
Hua Cui (2004)
A novel immunochromatographic electrochemical biosensor for highly sensitive and selective detection of trichloropyridinol, a biomarker of exposure to chlorpyrifos.
L. Wang (2011)
Signal-on electrochemiluminescence of biofunctional CdTe quantum dots for biosensing of organophosphate pesticides.
H. Liang (2014)
Effect of dissociation energy on ion formation and sensitivity of an analytical method for determination of chlorpyrifos in human blood, using gas chromatography–mass spectrometer (GC–MS in MS/MS)
S. Sinha (2006)
Development of Au nanoparticles dispersed carbon nanotube-based biosensor for the detection of paraoxon.
N. Jha (2010)
CdS nanocrystal-based electrochemiluminescence biosensor for the detection of low-density lipoprotein by increasing sensitivity with gold nanoparticle amplification.
G. Jie (2007)
Electrochemiluminescence acetylcholine biosensor based on biofunctional AMs-AChE-ChO biocomposite and electrodeposited graphene-Au-chitosan nanocomposite
X. Wu (2014)
Electrochemiluminescence biosensor based on conducting poly(5-formylindole) for sensitive detection of Ramos cells.
Guangming Nie (2013)
Glucose biosensor based on electrodeposition of platinum nanoparticles onto carbon nanotubes and immobilizing enzyme with chitosan-SiO2 sol–gel
Y. J. Zou (2016)
Microbial degradation of organophosphorus compounds.
B. Singh (2006)
Development of an immunochromatographic assay for the rapid detection of chlorpyrifos-methyl in water samples.
Xiude Hua (2010)
P. Wakeley (2013)
Surface-imprinted chitosan-coated magnetic nanoparticles modified multi-walled carbon nanotubes biosensor for detection of bovine serum albumin
H. Chen (2012)
Sensitive impedimetric DNA biosensor with poly(amidoamine) dendrimer covalently attached onto carbon nanotube electronic transducers as the tether for surface confinement of probe DNA.
N. Zhu (2010)
An amperometric biosensor based on acetylcholinesterase immobilized onto iron oxide nanoparticles/multi-walled carbon nanotubes modified gold electrode for measurement of organophosphorus insecticides.
N. Chauhan (2011)
Application of high-performance thin-layer chromatography for the detection of organophosphorus insecticides in human serum after acute poisoning.
K. Futagami (1997)
Catanionic surfactant ambient cloud point extraction and high-performance liquid chromatography for simultaneous analysis of organophosphorus pesticide residues in water and fruit juice samples
Ketsarin Seebunrueng (2011)
Detection of pesticides using an amperometric biosensor based on ferophthalocyanine chemically modified carbon paste electrode and immobilized bienzymatic system.
A. Ciucu (2003)
Disposable biosensor test for organophosphate and carbamate insecticides in milk.
Yaodong Zhang (2005)
Analysis of pesticides by chemiluminescence detection in the liquid phase
L. Gámiz-Gracia (2005)
Electrochemiluminescence sensor for sulfonylurea herbicide with molecular imprinting core–shell nanoparticles/chitosan composite film modified glassy carbon electrode
Huaifen Li (2013)
Study on Sensitization from Reactive Oxygen Species for Electrochemiluminescence of Luminol in Neutral Medium
H. Chu (2009)
Electrodeposited MnO 2 /Au composite film with improved electrocatalytic activity for oxidation of glucose and hydrogen peroxide, Eletrochim
Y J Yang (2010)
Molecularly imprinted electrochemical luminescence sensor based on signal amplification for selective determination of trace gibberellin A3.
J. Li (2012)
Microchip device with 64-site electrode array for multiplexed immunoassay of cell surface antigens based on electrochemiluminescence resonance energy transfer.
Meisheng Wu (2012)
Electrogeneration of polyluminol and chemiluminescence for new disposable reagentless optical sensors
A. Sassolas (2008)
Amperometric biosensors based on redox polymer-carbon nanotube-enzyme composites.
Pratixa P. Joshi (2005)
Paper-based chemiluminescence ELISA: lab-on-paper based on chitosan modified paper device and wax-screen-printing.
Shoumei Wang (2012)
M. M. Richter (2004)
Immobilization of acetylcholineesterase-choline oxidase on a gold-platinum bimetallic nanoparticles modified glassy carbon electrode for the sensitive detection of organophosphate pesticides, carbamates and nerve agents.
S. Upadhyay (2009)
Electrochemical sensor based on molecularly imprinted polymer film via sol-gel technology and multi-walled carbon nanotubes-chitosan functional layer for sensitive determination of quinoxaline-2-carboxylic acid.
Yukun Yang (2013)
Sensitization of the electrochemiluminescence of luminol from platinum sol modified electrode
H. H. Chu (2006)
Electrodeposited MnO2/Au composite film with improved electrocatalytic activity for oxidation of glucose and hydrogen peroxide
Y. J. Yang (2010)
Au-TiO2/Chit modified sensor for electrochemical detection of trace organophosphates insecticides.
Yunhe Qu (2008)
Mammalian toxicology of organophosphorus pesticides.
L. Sultatos (1994)
Pt-Pb alloy nanoparticle/carbon nanotube nanocomposite: a strong electrocatalyst for glucose oxidation
Hui-Fang Cui (2006)
Handbook of Pesticide Toxicology: Classes of Pesticides (EDs)
W. J. Hayes (1991)
Sensitization of the electrochemiluminescence of luminol from platinum sol modified electrode, Chin
H H Chu (2006)
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J. Mehta (2019)
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Application of DNA-Nanosensor for Environmental Monitoring: Recent Advances and Perspectives
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Xueling Shan (2020)
Recent progress in application of nanomaterial-enabled biosensors for ochratoxin A detection
Chengmei Jiang (2018)
Nanotechnology-based recent approaches for sensing and remediation of pesticides.
D. Rawtani (2018)
Determination of malation, methidathion, and chlorpyrifos ethyl pesticides using acetylcholinesterase biosensor based on Nafion/Ag@rGO-NH2 nanocomposites
M. Guler (2017)
Determination of Multiple Pesticides in Human Blood Using Modified QuEChERS Method with Fe3O4 Magnetic Nanoparticles and GC–MS
Tianxiao Yu (2016)
A convenient method for determination of malathion based on the resonance Rayleigh scattering enhancement
H. Wu (2017)
Enzymatic assays for the assessment of toxic effects of halogenated organic contaminants in water and food. A review.
Amaia Ereño Artabe (2020)
Recent advances in electrogenerated chemiluminescence biosensing methods for pharmaceuticals
Y. Zhang (2019)
Electrochemical luminescence sensor based on double suppression for highly sensitive detection of glyphosate
Liu Hanbiao (2020)
Selective Electrochemical Determination of Salicylic Acid in Wheat Using Molecular Imprinted Polymers
L. Ma (2017)
Visual detection of mixed organophosphorous pesticide using QD-AChE aerogel based microfluidic arrays sensor.
T. Hu (2019)
Detection of antibiotics in food: New achievements in the development of biosensors
M. Majdinasab (2020)
Recent advances in nanomaterial-based biosensors for antibiotics detection.
Lingyi Lan (2017)
Advanced carbon nanomaterials for electrochemiluminescent biosensor applications
Andrea Fiorani (2019)
Opportunities to advance sustainable design of nano-enabled agriculture identified through a literature review
J. Yin (2018)
Trends and Advances in Electrochemiluminescence Nanobiosensors
M. Rizwan (2018)
Sensitive electrogenerated chemiluminescence biosensors for protein kinase activity analysis based on bimetallic catalysis signal amplification and recognition of Au and Pt loaded metal-organic frameworks nanocomposites.
Zhiyong Yan (2018)
Ultrasound-accelerated synthesis of gold nanoparticles modified choline chloride functionalized graphene oxide as a novel sensitive bioelectrochemical sensor: Optimized meloxicam detection using CCD-RSM design and application for human plasma sample.
S. Bahrani (2018)
Multivariate nanocomposites for electrochemical sensing in the application of food
L. Lu (2019)
Constructing a simple and sensitive electrochemical sensor for the determination of NMN based on p-m-ABSA film modified glassy carbon electrode
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