← Back to Search
A Glycoprotein From Laminaria Japonica Induces Apoptosis In HT-29 Colon Cancer Cells.
Hiroe Go, H. Hwang, Taek-Jeong Nam
Published 2010 · Biology, Medicine
Download PDFAnalyze on Scholarcy
We isolated a novel glycoprotein from the brown alga Laminaria japonica that has antiproliferative effects on HT-29 colon cancer cells. We also identified the mechanism by which this glycoprotein, named LJGP, induces apoptosis. MTS assays showed that LJGP inhibited the proliferation of several cancer cell lines (AGS, HepG2, HT-29) in a dose-dependent manner. Especially in HT-29 cells, proliferation was significantly decreased. LJGP treatment on HT-29 displayed several apoptotic features, such as DNA fragmentation, sub-G1 arrest, caspase-3 activation, and PARP degradation. Consistent with sub-G1 arrest, LJGP decreased the expression of Cdk2, cyclin E, cyclin D1, PCNA, E2F-1, and phosphorylated pRb. Furthermore, the increase of p27 expression was observed. We also determined that LJGP-induced apoptosis leads to the formation of a death-induced signaling complex of Fas, FADD, and procaspase-8. LJGP induced the reduction of mitochondrial membrane potential with activation of the Bcl-2 family of proteins and caspase-9. These findings suggest that LJGP inhibits HT-29 cell proliferation by inducing apoptosis, which may be mediated via multiple pathways, including the Fas signaling pathway, the mitochondrial pathway, and cell cycle arrest. Therefore, LJGP can be a useful treatment option for colon cancer in humans.
This paper references
Grateloupia longifolia polysaccharide inhibits angiogenesis by downregulating tissue factor expression in HMEC‐1 endothelial cells
C. Zhang (2006)
3-Oxoolean-12-en-27-oic acid isolated from Aceriphyllum rossii induces caspase-8-dependent apoptosis in human promyelocytic leukemia HL-60 cells.
Sung-Su Kim (2009)
The TNF and TNF Receptor Superfamilies Integrating Mammalian Biology
R. Locksley (2001)
Anticoagulant fucoidan fractions from Fucus vesiculosus induce platelet activation in vitro.
J. Dürig (1997)
Anti-allergic effects of ethanol extracts from brown seaweeds
H. Samee (2008)
Genotype of thymidylate synthase likely to affect efficacy of adjuvant 5-FU based chemotherapy in colon cancer.
T. Matsui (2006)
Inhibitory Effect of Fucoidan on Huh7 Hepatoma Cells Through Downregulation of CXCL12
T. Nagamine (2009)
P53-mediated cell cycle arrest and apoptosis through a caspase-3-independent, but caspase-9-dependent pathway in oridonin-treated MCF-7 human breast cancer cells
Qiao Cui (2007)
Glycoprotein extraction from Laminaria japonica promotes IEC-6 cell proliferation.
Hiroe Go (2009)
Antitumor and antimetastatic activity of fucoidan, a sulfated polysaccharide isolated from the Okhotsk sea Fucus evanescens brown alga
T. V. Alekseyenko (2007)
Effect of a glycoprotein from Hizikia fusiformis on acetaminophen-induced liver injury.
Hye-Jeong Hwang (2008)
p27-Associated G1 arrest induced by hinokitiol in human malignant melanoma cells is mediated via down-regulation of pRb, Skp2 ubiquitin ligase, and impairment of Cdk2 function.
Shicheng Liu (2009)
Macrophage stimulation activity of the polysaccharide fraction from a marine alga (Porphyra yezoensis): structure-function relationships and improved solubility.
Y. Yoshizawa (1995)
Cytotoxicity‐dependent APO‐1 (Fas/CD95)‐associated proteins form a death‐inducing signaling complex (DISC) with the receptor.
F. Kischkel (1995)
Radiation therapy and combined modality treatment of gastrointestinal carcinomas.
Christoph Oehler (2006)
FADD, a novel death domain-containing protein, interacts with the death domain of fas and initiates apoptosis
A. Chinnaiyan (1995)
The Rb/E2F pathway: expanding roles and emerging paradigms.
J. Harbour (2000)
Proteases to die for.
V. Cryns (1998)
Anti-apoptotic Activity of Laminarin Polysaccharides and their Enzymatically Hydrolyzed Oligosaccharides from Laminaria japonica
Ki-hoon Kim (2005)
Trace Components and Functional Saccharides in Seaweed-1 -Changes in Proximate Composition and Trace Elements According to the Harvest Season and Places-
Cho Deuk-Moon (1995)
Potential antioxidant and anticoagulant capacity of low molecular weight fucoidan fractions extracted from Laminaria japonica.
J. Wang (2010)
Cytotoxic oxygenated desmosterols of the red alga Galaxaura marginata.
J. H. Sheu (1996)
Protective effects against H2O2-induced damage by enzymatic hydrolysates of an edible brown seaweed, sea tangle (Laminaria japonica).
P. Park (2009)
The effect of dietary seaweeds on 7,12-dimethyl-benz[a]anthracene-induced mammary tumorigenesis in rats.
I. Yamamoto (1987)
Switch in Fas-activated death signaling pathway as result of keratin 8/18-intermediate filament loss
S. Gilbert (2008)
Synthesized different derivatives of low molecular fucoidan extracted from Laminaria japonica and their potential antioxidant activity in vitro.
J. Wang (2009)
A New C-Terminal Cleavage Product of Procaspase-8, p30, Defines an Alternative Pathway of Procaspase-8 Activation
J. Hoffmann (2009)
p21 and p27 induction by silibinin is essential for its cell cycle arrest effect in prostate carcinoma cells
S. Roy (2007)
Gene expression of anti‐ and pro‐apoptotic proteins in malignant and normal plasma cells
M. Jourdan (2009)
Retinoic acid regulates Fas‐induced apoptosis in Jurkat T cells: reversal of mitogen‐mediated repression of Fas DISC assembly
N. Engedal (2009)
p27 modulates cell cycle progression and chemosensitivity in human malignant glioma.
U. Naumann (1999)
A Novel Protein That Interacts with the Death Domain of Fas/APO1 Contains a Sequence Motif Related to the Death Domain (*)
M. Boldin (1995)
Antioxidant capacity and lipophilic content of seaweeds collected from the Qingdao coastline.
H. Huang (2004)
Proliferation, cell cycle and apoptosis in cancer
G. Evan (2001)
Deguelin inhibits the growth of colon cancer cells through the induction of apoptosis and cell cycle arrest.
G. Murillo (2002)
Antithrombin activity of an algal polysaccharide.
F. Trento (2001)
Death receptors: signaling and modulation.
A. Ashkenazi (1998)
Cytochrome c and dATP-Dependent Formation of Apaf-1/Caspase-9 Complex Initiates an Apoptotic Protease Cascade
Peng Li (1997)
Chemoprotective effects of a protein from the red algae Porphyra yezoensis on acetaminophen‐induced liver injury in rats
H. Hwang (2008)
This paper is referenced by
Marine Niche: Applications in Pharmaceutical Sciences: Translational Research
Chandrashekar Mootapally (2020)
Effect of subcritical water hydrolysate in the brown seaweed Saccharina japonica as a potential antibacterial agent on food-borne pathogens
Aviannie Meillisa (2013)
Bioactive Compounds from Marine Sources for Gastrointestinal Cancer Treatment
Se-kwon Kim (2012)
Correlation between TAP detection and common digestive tract precancerous lesions
C. Sun (2018)
Inhibitory effects of glycoprotein isolated from Laminaria japonica on lipopolysaccharide-induced pro-inflammatory mediators in BV2 microglial cells.
Hye-Young Park (2011)
Antioxidant and DNA protection activities of a glycoprotein isolated from a seaweed, Saccharina japonica
E. Kim (2012)
Clues for Cancer from Ocean-Derived Molecules and Role of In Silico Techniques in Anticancer Drug Discovery
G. Davis (2012)
Exogenous natural glycoprotein multiple mechanisms of anti-tumor activity.
Hong-Liang Yuan (2015)
Marine algal natural products with anti-oxidative, anti-inflammatory, and anti-cancer properties
J. Lee (2013)
C Enhancement of 5-Flurouracil Uptake by Insulin in Human Colonic Cancer
Hamid Naji Obeid (2014)
From Marine Origin to Therapeutics: The Antitumor Potential of Marine Algae-Derived Compounds
Celso Alves (2018)
In vitro and in vivo anticancer activity of a novel puerarin nanosuspension against colon cancer, with high efficacy and low toxicity.
Yancai Wang (2013)
Molecular Size Fractions of Bay Leaf (Laurus nobilis) Exhibit Differentiated Regulation of Colorectal Cancer Cell Growth In Vitro
L. Bennett (2013)
A review of the components of brown seaweeds as potential candidates in cancer therapy
E. Hussain (2016)
High-Throughput Screening to Identify Plant Derived Human LDH-A Inhibitors
S. Deiab (2013)
Prolific Anticancer Bioactivity of AlgalExtracts (Review)
N. Sharif (2014)
Biological activities of two macroalgae from Adriatic coast of Montenegro
M. Kosanić (2015)
Induction of apoptosis by a peptide from Porphyra yezoensis: Regulation of the insulin-like growth factor I receptor signaling pathway in MCF-7 cells
S. Park (2014)
Capsosiphon fulvescens glycoprotein reduces AGS gastric cancer cell migration by downregulating transforming growth factor-β 1 and integrin expression
Y. Kim (2013)
Dictyopteris undulata Extract Induces Apoptosis via Induction of Endoplasmic Reticulum Stress in Human Colon Cancer Cells
K. Kang (2014)
PPY induces cell cycle arrest and inhibits the IGF-IR signaling pathway. Cell proliferation studies revealed that PPY induced cell death in a dose-dependent manner. Expression levels of IGF-IR were decreased in MCF-7 cells by PPY in a dose-depen-
Su-jin Park (2014)
Inhibition of Acute Phase Inflammation by Laminaria japonica through Regulation of iNOS-NF-κB Pathway
S. Park (2013)
Marine Algae and Chronic Diseases
K. Senthilkumar (2015)
Looking Beyond the Terrestrial: The Potential of Seaweed Derived Bioactives to Treat Non-Communicable Diseases
Kenneth G. Collins (2016)
Anticancer effects of traditional Chinese herbs with phlegm-eliminating properties - An overview.
Li-juan Xiu (2015)
Protein extracted from Porphyra yezoensis prevents cisplatin-induced nephrotoxicity by downregulating the MAPK and NF-κB pathways.
In-Hye Kim (2018)
Chemical and mass spectrometry characterization of the red alga Pyropia yezoensis chemoprotective protein (PYP): protective activity of the N-terminal fragment of PYP1 against acetaminophen-induced cell death in Chang liver cells.
Youn Hee Choi (2015)
Perspective Studies on Novel Anticancer Drugs from Natural Origin:A Comprehensive Review
S. Saeidnia (2014)
Chemical Composition and Antioxidant and Antibacterial Activities of an Essential Oil Extracted from an Edible Seaweed, Laminaria japonica L.
J. Patra (2015)
Laminaria japonica Extract, an Inhibitor of Clavibater michiganense Subsp. Sepedonicum
J. Cai (2014)
Dietary Hizikia fusiformis glycoprotein-induced IGF-I and IGFBP-3 associated to somatic growth, polyunsaturated fatty acid metabolism, and immunity in juvenile olive flounder Paralichthys olivaceus.
Youn Hee Choi (2014)
Utilisation des algues dans les compléments alimentaires : usages et justifications scientifiques
Amandine Ollier (2017)See more