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Engineered Cobalt Oxide Nanoparticles Readily Enter Cells.
E. Papis, F. Rossi, M. Raspanti, I. Dalle-Donne, G. Colombo, A. Milzani, G. Bernardini, R. Gornati
Published 2009 · Chemistry, Medicine
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Magnetic nanoparticles (NPs) have great potential for applications not only as catalysts or energy storage devices, but also in biomedicine, as contrast enhancement agents for magnetic resonance imaging, or for drug delivery. The same characteristics that make cobalt-based NPs so attractive raise serious questions about their safety. In this context, we investigated Co3O4-NPs. Believing that the characterization of NPs is relevant for understanding their biological activity, we analyzed them by atomic force and electron microscopy to define size, shape, and aggregation. To clarify whether their biological effects could be due to a potential release of cobalt ions, we evaluated spontaneous dissolution in different media. To determine their potential toxicity to human cells, we measured cell viability and ROS formation in two human cell lines using CoCl2 for comparison. Co3O(4)-NPs induced a concentration- and time-dependent impairment of cellular viability, although cobalt ions were more toxic. We also demonstrated that cobalt causes a rapid induction of ROS if supplied in the form of Co3O4-NPs rather than as ions. Moreover, we evaluated the cellular uptake of NPs. Interestingly, Co3O4-NPs are able to enter the cell very rapidly, remaining confined in vesicles inside the cytoplasm. They were found also inside the cell nuclei, though less frequently.
This paper references
Comparative genotoxicity of cobalt nanoparticles and ions on human peripheral leukocytes in vitro.
R. Colognato (2008)
Sinusoidal endothelial endocytosis of low density lipoprotein-gold conjugates in perfused livers of ethinyl-estradiol treated rats.
D. Handley (1983)
Cobalt nanoparticles as a novel magnetic resonance contrast agent--relaxivities at 1.5 and 3 Tesla.
L. Parkes (2008)
Modification of neutrophil adhesion to human endothelial cell line in acute ischemic stroke by dipyridamole and candesartan
H. Hallevi (2007)
Magnetic nanoparticle probes
L. LaConte (2005)
Superparamagnetic iron oxide nanoparticles for bio-medical applications
D. Kim (2001)
Metallic Nanoparticles Exhibit Paradoxical Effects on Oxidative Stress and Pro-Inflammatory Response in Endothelial Cells in Vitro
K. Peters (2007)
Investing in nanotechnology
Robert Paull (2003)
Critical Evaluation of ECV304 as a Human Endothelial Cell Model Defined by Genetic Analysis and Functional Responses: A Comparison with the Human Bladder Cancer Derived Epithelial Cell Line T24/83
J. Brown (2000)
Endocytosis by endothelial phagocytes: uptake of bovine serum albumin-gold conjugates in bone marrow.
J. S. Geoffroy (1984)
Exposure of engineered nanoparticles to human lung epithelial cells: influence of chemical composition and catalytic activity on oxidative stress.
Ludwig K. Limbach (2007)
In Search of the Most Relevant Parameter for Quantifying Lung Inflammatory Response to Nanoparticle Exposure: Particle Number, Surface Area, or What?
K. Wittmaack (2007)
Nanotechnology: the next big thing, or much ado about nothing?
A. Maynard (2007)
Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm.
T. Xia (2006)
Formation of nucleoplasmic protein aggregates impairs nuclear function in response to SiO2 nanoparticles.
Min Chen (2005)
Induction of protein oxidation by cobalt and chromium ions in human U937 macrophages.
A. Petit (2005)
High-content screening as a universal tool for fingerprinting of cytotoxicity of nanoparticles.
E. Jan (2008)
Retrospective analysis of 4-week inhalation studies in rats with focus on fate and pulmonary toxicity of two nanosized aluminum oxyhydroxides (boehmite) and pigment-grade iron oxide (magnetite): the key metric of dose is particle mass and not particle surface area.
J. Pauluhn (2009)
Neuroprotective effect of cobalt chloride on hypobaric hypoxia-induced oxidative stress
K. Shrivastava (2008)
Cobalt chloride induces PC12 cells apoptosis through reactive oxygen species and accompanied by AP‐1 activation
W. Zou (2001)
Evaluation of the in vitro direct and indirect genotoxic effects of cobalt compounds using the alkaline comet assay. Influence of interdonor and interexperimental variability.
M. De Boeck (1998)
Recherche in vitro sur la toxicologie des nanoparticules au Joint Research Center
E. Sabbioni (2006)
Nanotoxicology: An Emerging Discipline Evolving from Studies of Ultrafine Particles
G. Oberdörster (2005)
Toxic Potential of Materials at the Nanolevel
A. Nel (2006)
Gene expression in nanotoxicology: A search for biomarkers of exposure to cobalt particles and ions
E. Papis (2007)
Induction of Inflammation in Vascular Endothelial Cells by Metal Oxide Nanoparticles: Effect of Particle Composition
A. Gojova (2007)
Possible exploitation of magnetic nanoparticle–cell interaction for biomedical applications
C. Berry (2005)
Gene expression in nanotoxicology research: analysis by differential display in BALB3T3 fibroblasts exposed to cobalt particles and ions.
E. Papis (2007)
Interfacing biology with nanoparticles
S. Mandal (2005)
Shape-controlled synthesis and properties of uniform spinel cobalt oxide nanocubes
X. Liu (2005)
Translocation through the nuclear pore: Kaps pave the way
R. Peters (2009)
Optical recognition of CO and H2 by use of gas-sensitiveAu–Co3O4 composite films
M. Ando (1997)
Medical application of functionalized magnetic nanoparticles.
A. Ito (2005)
The ECV‐304 cell‐line: should it be used?
H. Hallevi (2008)
Environmentally responsible development of nanotechnology.
M. Roco (2005)
APPLICATIONS OF MAGNETIC NANOPARTICLES IN BIOMEDICINE
C. Bárcena (2003)
Beaded Cobalt Oxide Nanoparticles along Carbon Nanotubes: Towards More Highly Integrated Electronic Devices
L. Fu (2005)
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Magnetic nanovectors for drug delivery.
J. Klostergaard (2012)
Neutral red retention time assay in determination of toxicity of nanoparticles.
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Synthesis, characterization and toxicological evaluation of iron oxide nanoparticles in human lung alveolar epithelial cells.
S. Dwivedi (2014)
Linking an α-tocopherol derivative to cobalt(0) nanomagnets: magnetically responsive antioxidants with superior radical trapping activity and reduced cytotoxicity.
C. Viglianisi (2014)
Anticancer and immunostimulatory role of encapsulated tumor antigen containing cobalt oxide nanoparticles
S. Chattopadhyay (2013)
A study of the mechanism of in vitro cytotoxicity of metal oxide nanoparticles using catfish primary hepatocytes and human HepG2 cells.
Y. Wang (2011)
Surgical Magnetic Systems and Tracers for Cancer Staging
N. T. Thanh (2012)
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Cellular internalisation of dissolved cobalt ions from ingested CoFe2O4 nanoparticles: in vivo experimental evidence
S. Trieste (2012)
D-amino acid oxidase-nanoparticle system: a potential novel approach for cancer enzymatic therapy.
A. Bava (2013)
Biosynthesis of cobalt oxide nanoparticles using endophytic fungus Aspergillus nidulans.
Ajuy Sundar Vijayanandan (2018)
Heparin and Carboxymethylchitosan Metal Nanoparticles: An Evaluation of Their Cytotoxicity
A. Bava (2013)
21 View on the Magnetic Properties of Nanoparticles Co m ( m = 6 , 8 , 10 , 12 , 14 ) and Co 6 O n ( n = 1-9 )
Jelena Tamulien (2017)
Deriviation of Terrestrial Predicted No-Effect Concentration (PNEC) for Cobalt Oxide Nanomaterial
Sirine Bouguerra (2017)
Internalisation of engineered nanoparticles into mammalian cells in vitro: influence of cell type and particle properties
W. Busch (2011)
Nanoparticle-based immunotherapy: state of the art and future perspectives
M. Di Gioacchino (2020)
Nanoparticles and their antimicrobial properties against pathogens including bacteria, fungi, parasites and viruses.
Arezou Khezerlou (2018)
Photodynamic therapy, facile synthesis, and effect of sintering temperature on the structure, morphology, optical properties, and anticancer activity of Co3O4 nanocrystalline materials in the HepG2 cell line
Seemab Iqbal (2020)
Crystallinity depends on choice of iron salt precursor in the continuous hydrothermal synthesis of Fe–Co oxide nanoparticles
J. Liu (2017)
Exposure to sublethal concentrations of Co3O4 and Mn2O3 nanoparticles induced elevated metal body burden in Daphnia magna.
M. Heinlaan (2017)
BIOGEOCHEMICAL BEHAVIOUR OF HEAVY METALS IN SOIL-PLANT SYSTEM
M. Shahid (2017)
Effects of Cobalt Nanoparticles on Human T Cells In Vitro
H. Jiang (2011)
Ameliorative effects of melatonin against nano and ionic cobalt induced genotoxicity in two in vivo Drosophila assays
H. Ertuğrul (2019)
Interaction with culture medium components, cellular uptake and intracellular distribution of cobalt nanoparticles, microparticles and ions in Balb/3T3 mouse fibroblasts
E. Sabbioni (2014)
Cobalt nanoparticles for biomedical applications: Facile synthesis, physiochemical characterization, cytotoxicity behavior and biocompatibility
S. Ansari (2017)
Surface chemistry modulated introduction of multifunctionality within Co3O4 nanocubes
M. Pal (2015)
Food Nanoscience and Nanotechnology
H. Hernández-Sánchez (2015)
Phosphonomethyl iminodiacetic acid-conjugated cobalt oxide nanoparticles liberate Co++ ion-induced stress associated activation of TNF-α/p38 MAPK/caspase 8-caspase 3 signaling in human leukemia cells
S. Chattopadhyay (2014)
Nanomaterials as nanocarriers: a critical assessment why these are multi-chore vanquisher in breast cancer treatment
Sania Naz (2018)
Gamma-radiolysis-assisted cobalt oxide nanoparticle formation.
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