Online citations, reference lists, and bibliographies.
← Back to Search

In Vitro Nanotoxicity Of Single-walled Carbon Nanotube-dendrimer Nanocomplexes Against Murine Myoblast Cells.

J. Cancino, I. M. Paino, K. C. Micocci, H. S. Selistre-de-Araújo, V. Zucolotto
Published 2013 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
Single-wall carbon nanotubes (SWCNTs) and polyamidoamine dendrimers (PAMAM) have been proposed for a variety of biomedical applications. The combination of both molecules makes this new composite nanomaterial highly functionalizable and versatile to theranostic and drug-delivery systems. However, recent toxicological studies have shown that nanomaterials such as SWCNTs and PAMAM may have high toxicity in biological environments. Aiming to elucidate such behavior, in vitro studies with different cultured cells have been conducted in the past few years. This study focuses on the effects of SWCNT-PAMAM nanomaterials and their individual components on the C2C12 murine cell line, which is a mixed population of stem and progenitor cells. The interactions between the cells and the nanomaterials were studied with different techniques usually employed in toxicological analyses. The results showed that SWCNT-PAMAM and PAMAM inhibited the proliferation and caused DNA damage of C2C12 cells. Data from flow cytometry revealed a less toxicity in C2C12 cells exposed to SWCNT compared to the other nanomaterials. The results indicated that the toxicity of SWCNT, SWCNT-PAMAM and PAMAM in C2C12 cells can be strongly correlated with the charge of the nanomaterials.
This paper references
10.1007/s11095-008-9811-0
Crucial Functionalizations of Carbon Nanotubes for Improved Drug Delivery: A Valuable Option?
Giorgia Pastorin (2008)
10.1016/j.toxlet.2008.11.019
Genotoxicity of nanomaterials: DNA damage and micronuclei induced by carbon nanotubes and graphite nanofibres in human bronchial epithelial cells in vitro.
Hanna K. Lindberg (2009)
10.3109/17435390.2011.629748
A new strategy to investigate the toxicity of nanomaterials using Langmuir monolayers as membrane models
Juliana Cancino (2013)
10.1002/ELAN.200603775
Electroactive Nanostructured Membranes (ENM): Synthesis and Electrochemical Properties of Redox Mediator‐Modified Gold Nanoparticles Using a Dendrimer Layer‐by‐Layer Approach
F. Crespilho (2007)
10.1039/c1cs15188f
Toxicity of nanomaterials.
S. Sharifi (2012)
10.1289/ehp.7339
Nanotoxicology: An Emerging Discipline Evolving from Studies of Ultrafine Particles
G. Oberdörster (2005)
10.1093/toxsci/kfq372
The new toxicology of sophisticated materials: nanotoxicology and beyond.
A. Maynard (2011)
10.1016/j.yrtph.2011.07.008
Evaluation of the genotoxic potential of single-wall carbon nanotubes by using a battery of in vitro and in vivo genotoxicity assays.
M. Naya (2011)
10.1021/mp2006054
Cationic PAMAM dendrimers disrupt key platelet functions.
Clinton F Jones (2012)
10.1182/BLOOD.V87.3.1179.BLOODJOURNAL8731179
Detection of altered membrane phospholipid asymmetry in subpopulations of human red blood cells using fluorescently labeled annexin V.
F. Kuypers (1996)
10.1016/J.TOXLET.2004.08.015
Effect of single wall carbon nanotubes on human HEK293 cells.
D. Cui (2005)
Differences in target cell DNA fragmentation induced by mouse cytotoxic T lymphocytes and natural killer cells.
R. C. Duke (1986)
10.1016/J.CARBON.2007.06.054
Human lung epithelial cells show biphasic oxidative burst after single-walled carbon nanotube contact
K. Pulskamp (2007)
10.1016/J.ELECOM.2006.08.052
Functionalization of multi-walled carbon nanotubes with poly(amidoamine) dendrimer for mediator-free glucose biosensor
Yunlong Zeng (2007)
10.1155/JBB/2006/27601
Parkinson's Disease in Relation to Pesticide Exposure and Nuclear Encoded Mitochondrial Complex I Gene Variants
E. Corder (2006)
10.1016/j.mrgentox.2011.02.008
Effects of single and multi walled carbon nanotubes on macrophages: cyto and genotoxicity and electron microscopy.
M. L. Di Giorgio (2011)
10.5101/NBE.V2I1.P61-66
Morphological Observation of Interaction between PAMAM Dendrimer Modified Single Walled Carbon Nanotubes and Pancreatic Cancer Cells
Dongfeng Chen (2010)
10.1016/J.CARBON.2005.10.011
THE BIOCOMPATIBILITY OF CARBON NANOTUBES
S. Smart (2006)
10.3109/10715760903300691
Role of oxidative damage in toxicity of particulates
P. Møller (2010)
10.1007/s10753-010-9182-7
Carbon Nanotubes Elicit DNA Damage and Inflammatory Response Relative to Their Size and Shape
K. Yamashita (2010)
10.1146/annurev-anchem-062011-143134
Assessing nanoparticle toxicity.
S. Love (2012)
10.1126/SCIENCE.1060928
Carbon Nanotubes--the Route Toward Applications
R. Baughman (2002)
10.1016/0014-4827(88)90265-0
A simple technique for quantitation of low levels of DNA damage in individual cells.
N. Singh (1988)
10.1039/b901839p
Biomedical applications of dendrimers: a tutorial.
Meredith A. Mintzer (2011)
10.1016/S1388-1981(02)00326-8
Phospolipase A2 and apoptosis.
M. Taketo (2002)
10.1016/j.drudis.2010.01.009
Dendrimer-based drug and imaging conjugates: design considerations for nanomedical applications.
A. R. Menjoge (2010)
10.1016/j.tiv.2011.04.001
Effect of exposure conditions on SWCNT-induced inflammatory response in human alveolar epithelial cells.
Rena Baktur (2011)
10.1080/15287390701601251
Single-walled Carbon Nanotubes: Geno- and Cytotoxic Effects in Lung Fibroblast V79 Cells
E. Kisin (2007)
10.1016/j.biomaterials.2009.04.009
NanoGenotoxicology: the DNA damaging potential of engineered nanomaterials.
N. Singh (2009)
10.3390/ijms12053303
Nanomedicine: Application Areas and Development Prospects
H. Boulaiz (2011)
10.1016/j.addr.2009.03.010
Effects of nanomaterial physicochemical properties on in vivo toxicity.
Kristin L Aillon (2009)
10.1016/j.taap.2011.11.010
Nanotoxicology and in vitro studies: the need of the hour.
Sumit Arora (2012)
10.1016/j.tiv.2012.03.007
Oxidative stress responses to carboxylic acid functionalized single wall carbon nanotubes on the human intestinal cell line Caco-2.
S. Pichardo (2012)
10.1021/NL060177C
Oops they did it again! Carbon nanotubes hoax scientists in viability assays.
J. M. Wörle-Knirsch (2006)
10.1002/em.20545
Carbon nanotubes induce oxidative DNA damage in RAW 264.7 cells
Lucia Migliore (2010)
10.1182/BLOOD.V84.5.1415.BLOODJOURNAL8451415
Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis.
G. Koopman (1994)
10.1126/SCIENCE.1114397
Toxic Potential of Materials at the Nanolevel
A. Nel (2006)
Nanoparticle carbon black driven DNA damage induces growth arrest and AP-1 and NFkappaB DNA binding in lung epithelial A549 cell line.
R. Mróz (2007)
Multi-walled carbon nanotubes induce cytotoxicity, genotoxicity and apoptosis in normal human dermal fibroblast cells.
A. Patlolla (2010)
10.1038/nnano.2010.164
An index for characterization of nanomaterials in biological systems.
Xin-Rui Xia (2010)
10.1021/bm900266r
Interaction of nanoparticles with cells.
V. Mailaender (2009)
10.1016/j.toxlet.2012.09.025
Cyto and genotoxicity of gold nanoparticles in human hepatocellular carcinoma and peripheral blood mononuclear cells.
I. M. Paino (2012)
10.3109/10408440903453074
A review of the in vivo and in vitro toxicity of silver and gold particulates: Particle attributes and biological mechanisms responsible for the observed toxicity
H. Johnston (2010)
10.1007/s11010-011-0726-4
Extracellular ATP signaling during differentiation of C2C12 skeletal muscle cells: role in proliferation
T. Martinello (2011)
10.1016/J.TOXLET.2004.11.004
Multi-walled carbon nanotube interactions with human epidermal keratinocytes.
N. Monteiro-Riviere (2005)
10.1016/j.bbagen.2010.04.007
Toxicology of engineered nanomaterials: focus on biocompatibility, biodistribution and biodegradation.
A. Kunzmann (2011)
10.1093/TOXSCI/KFM279
How meaningful are the results of nanotoxicity studies in the absence of adequate material characterization?
D. Warheit (2008)
10.1155/JBB/2006/51516
Nanomedicine: Techniques, Potentials, and Ethical Implications
Mette Ebbesen (2006)
10.1002/SMLL.200700378
Size-dependent cytotoxicity of gold nanoparticles.
Y. Pan (2007)
10.1016/j.ijpharm.2010.04.027
Dendrimer toxicity: Let's meet the challenge.
K. Jain (2010)
10.1002/anie.201201991
Degree of chemical functionalization of carbon nanotubes determines tissue distribution and excretion profile.
K. Al-Jamal (2012)



This paper is referenced by
10.1016/j.cbi.2019.04.036
Toxicity of carbon-based nanomaterials: Reviewing recent reports in medical and biological systems.
Rasoul Madannejad (2019)
10.1002/wnan.1250
Dendrimers as high relaxivity MR contrast agents.
M. Longmire (2014)
10.1039/c3an02280c
Chip based single cell analysis for nanotoxicity assessment.
P. Shah (2014)
10.1002/pat.4874
Fabrication of random and aligned electrospun nanofibers containing graphene oxide for skeletal muscle cells scaffold
Thiers Massami Uehara (2020)
10.3390/ph6111361
Nanoparticles as Drug Delivery Systems in Cancer Medicine: Emphasis on RNAi-Containing Nanoliposomes
Mónica Rivera Díaz (2013)
10.1007/978-81-322-2175-3_4
Synthetic (Inorganic) Nanoparticles Based Lung Cancer Diagnosis and Therapy
Abhijit Bandyopadhyay (2015)
10.1016/B978-0-12-809717-5.00013-0
Dendrimers in Targeting and Delivery of Drugs
P. Kesharwani (2017)
10.1016/j.chemosphere.2018.09.012
Gold-based nanospheres and nanorods particles used as theranostic agents: An in vitro and in vivo toxicology studies.
J. Cancino-Bernardi (2018)
10.1021/JE400913Z
Evaluation of Adsorption Characteristics of Multiwalled Carbon Nanotubes Modified by a Poly(propylene imine) Dendrimer in Single and Multiple Dye Solutions: Isotherms, Kinetics, and Thermodynamics
Ladan Eskandarian (2014)
10.1016/j.jddst.2020.101867
A review of imperative concerns against clinical translation of nanomaterials: Unwanted biological interactions of nanomaterials cause serious nanotoxicity
Zahid Hussain (2020)
10.1016/J.PROGPOLYMSCI.2013.07.005
Dendrimer as nanocarrier for drug delivery
P. Kesharwani (2014)
10.1016/j.jconrel.2019.07.016
Two dimensional carbon based nanocomposites as multimodal therapeutic and diagnostic platform: A biomedical and toxicological perspective.
Namdev L. Dhas (2019)
10.1093/mutage/geu035
Applications of the comet assay in particle toxicology: air pollution and engineered nanomaterials exposure.
P. Møller (2015)
10.1093/toxsci/kfy100
Genotoxicity Assessment of Nanomaterials: Recommendations on Best Practices, Assays, and Methods
R. Elespuru (2018)
10.25148/ETD.FI15032160
Development of a Lab-on-a-Chip Device for Rapid Nanotoxicity Assessment In Vitro
Pratikkumar Shah (2014)
10.1021/es5038194
Structure-function relationship of PAMAM dendrimers as robust oil dispersants.
N. K. Geitner (2014)
10.5958/2231-5713.2015.00008.2
A Review- Emerging Use of Nano-Based Carriers in Diagnosis and Treatment of Cancer-Novel Approaches
Yaqub Khan (2015)
10.1007/978-81-322-2175-3
Nanoparticles in Lung Cancer Therapy - Recent Trends
Abhijit Bandyopadhyay (2014)
10.1016/J.VACUUM.2018.02.025
Cytotoxic, genetic and statistical analytical evaluation of functionalized CNTs with C2C12 cells
Rizwan Wahab (2018)
Development of genistein-PEGylated silica hybrid nanomaterials with enhanced antioxidant and antiproliferative properties on HT29 human colon cancer cells.
H. Pool (2018)
10.1007/978-94-017-8739-0_13
Stem cells and nanomaterials.
M. Hofmann (2014)
10.1039/C3RA47184E
Copper immobilized on nano-silica triazine dendrimer (Cu(II)-TD@nSiO2) catalyzed synthesis of symmetrical and unsymmetrical 1,3-diynes under aerobic conditions at ambient temperature
M. Nasr-Esfahani (2014)
Estudo do papel da ADAM9 na disseminação tumoral via sistema linfático: possível alvo farmacológico
K. C. Micocci (2014)
10.1016/j.ceramint.2020.01.072
γ-Fe2O3 nanoparticles embedded in nanohydroxyapatite matrix for magnetic hyperthermia and in vitro osteoblast cell studies
Juan A. Ramos-Guivar (2020)
10.1371/journal.pone.0167796
Interaction Potency of Single-Walled Carbon Nanotubes with DNAs: A Novel Assay for Assessment of Hazard Risk
Chunhe Yao (2016)
10.1016/j.drudis.2017.06.009
Dendrimer nanohybrid carrier systems: an expanding horizon for targeted drug and gene delivery.
P. Kesharwani (2018)
10.1016/j.envpol.2017.12.016
2,3,7,8-Tetrachlorodibenzo-p-dioxin induces alterations in myogenic differentiation of C2C12 cells.
H. Q. Xie (2018)
10.1016/j.etap.2014.12.012
Poly(vinyl alcohol)-coated silver nanoparticles: activation of neutrophils and nanotoxicology effects in human hepatocarcinoma and mononuclear cells.
I. M. Paino (2015)
A biophysical understanding of the applications and implications of nanomaterials
N. K. Geitner (2014)
10.2174/1573413715666190618162321
Drug Delivery Using Theranostics: An Overview of Its Use, Advantages and Safety Assessment
B. G. Chiari-Andréo (2020)
Semantic Scholar Logo Some data provided by SemanticScholar