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A Fiber-optic Evanescent Wave DNA Biosensor Based On Novel Molecular Beacons.

X. Liu, W. Tan
Published 1999 · Chemistry, Medicine

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We have prepared a novel optical fiber evanescent wave DNA biosensor using a newly developed molecular beacon DNA probe. The molecular beacons (MB) are oligonucleotide probes that become fluorescent upon hybridization with target DNA/RNA molecules. Biotinylated MBs have been designed and immobilized on an optical fiber core surface via biotin-avidin or biotin-streptavidin interactions. The DNA sensor based on a MB does not need labeled analyte or intercalation reagents. It can be used to directly detect, in real-time, target DNA/RNA molecules without using competitive assays. The sensor is rapid, stable, highly selective, and reproducible. We have studied the hybridization kinetics of the immobilized MB by changing the ionic strength of the hybridization solution and target DNA concentration. Our result shows divalent cations play a more important role than monovalent cations in stabilizing the MB stem hybrids and in accelerating the hybridization reaction with target DNA/RNA molecules. The concentration detection limit of the MB evanescent wave biosensor is 1.1 nM. The MB DNA biosensor has been applied to the analysis of specific gamma-actin mRNA sequences amplified by polymerase chain reaction.
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Molecular beacons for bioanalytical applications.
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G. Habl (2005)
Investigation of Fiber Optic Sensor for Monitoring of Ammonia
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Development of novel dye-doped silica nanoparticles for biomarker application.
S. Santra (2001)
Molecular beacons in biomedical detection and clinical diagnosis.
Youngmi Kim (2008)
Low-cost optical instrumentation for biomedical measurements
Y. Kostov (2000)
Optical Nanosensors and Nanobiosensors
Brian M. Cullum (2009)
Molekulartechnische DNA‐Modifizierung: Molecular Beacons
K. Wang (2009)
Non-conventional Generation and Transformation of Response
Alexander Demchenko (2015)
Optical DNA biosensor based on molecular beacon immobilized on sol-gel membrane
J. Li (2001)
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M. Raab (2008)
Techniques related to graphene biosensors and their potential combination with optical fibres
Chunsheng Yan (2011)
Molecular beacons immobilized within suspended core optical fiber for specific DNA detection.
Linh Viet Nguyen (2012)
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Progress of Spectral Probes for Nucleic Acids
Y. Wang (2003)
Chemical modification of silica surface by immobilization of functional groups for extractive concentration of metal ions.
P. Jal (2004)
Development of a monolithic total internal reflection-based biochip utilizing a microprism array for fluorescence sensing
S. Huang (2005)
Chapter 3 – Planar Waveguides for Fluorescence Biosensors
K. Sapsford (2002)
Aptamers: molecular tools for analytical applications
T. Mairal (2008)
Electrochemical Capacitance DNA Sensing at Hairpin-Modified Au Electrodes
J. Rivera-Gandía (2011)
Optical Fiber Immunosensors and Genosensors for the Detection of Viruses
Yael Liebes (2014)
Microbial forensics: fiber optic microarray subtyping of Bacillus anthracis
Jason R. E. Shepard (2009)
Ultrasensitive optical DNA biosensor based on surface immobilization of molecular beacon by a bridge structure.
J. Li (2001)
Molecular beacons: colorful analysis of nucleic acids
Jacqueline A. M. Vet (2002)
Nanostructured Probes for RNA Detection in Living Cells
P. Santangelo (2005)
Triple functional DNA-protein conjugates: Signal probes for Pb(2+) using evanescent wave-induced emission.
R. Wang (2015)
Using force spectroscopy analysis to improve the properties of the hairpin probe
L. Yang (2007)
The Development of Novel Miniaturized Immuno-sensing Devices: A Review of a Small Technology with a Large Future
A. D. Sheehan (2003)
BeadCons: Detection of Nucleic Acid Sequences by Flow Cytometry
D. Horejsh (2005)
Molecular aptamer beacons for real-time protein recognition.
J. Li (2002)
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