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On-site Detection Of TNT With A Portable Fiber Optic Biosensor

L. Shriver-Lake, B. L. Donner, F. Ligler
Published 1997 · Environmental Science

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A portable fiber optic biosensor was employed for on-site analysis of the explosive trinitrotoluene (TNT) in groundwater. Two military bases, Umatilla Army Depot and Naval Submarine Base Bangor, contain sites where the groundwater has been contaminated with TNT. Samples from monitoring wells at these sites were split and analyzed on-site using the fiber optic biosensor and in a laboratory by U.S. EPA SW-846 Method 8330 (reverse-phase high-performance liquid chromatography). The fiber optic biosensor is small (6.5 in. × 4.5 in. × 3.5 in., 2.5 lb), portable, and able to monitor four optical probes simultaneously. A competitive, fluorescent immunoassay was performed on the surface of the optical fiber probe. The fiber optic biosensor was able to detect 20 μg/L TNT in less than 16 min simultaneously on four probes.



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