← Back to Search
Molecular Characterization Of The Cytotoxic Mechanism Of Multiwall Carbon Nanotubes And Nano-onions On Human Skin Fibroblast.
L. Ding, J. Stilwell, T. Zhang, O. Elboudwarej, Huijian Jiang, J. Selegue, Patrick A Cooke, J. Gray, F. Chen
Published 2005 · Materials Science, Medicine
Save to my Library
Download via 🐼 PaperPanda Download via oaDOI Download via OAB Download via LibKey Download via Google Google ScholarAnalyze on Scholarcy Visualize in Litmaps
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
The increasing use of nanotechnology in consumer products and medical applications underlies the importance of understanding its potential toxic effects to people and the environment. Although both fullerene and carbon nanotubes have been demonstrated to accumulate to cytotoxic levels within organs of various animal models and cell types and carbon nanomaterials have been exploited for cancer therapies, the molecular and cellular mechanisms for cytotoxicity of this class of nanomaterial are not yet fully apparent. To address this question, we have performed whole genome expression array analysis and high content image analysis based phenotypic measurements on human skin fibroblast cell populations exposed to multiwall carbon nano-onions (MWCNOs) and multiwall carbon nanotubes (MWCNTs). Here we demonstrate that exposing cells to MWCNOs and MWCNTs at cytotoxic doses induces cell cycle arrest and increases apoptosis/necrosis. Expression array analysis indicates that multiple cellular pathways are perturbed after exposure to these nanomaterials at these doses, with material-specific toxigenomic profiles observed. Moreover, there are also distinct qualitative and quantitative differences in gene expression profiles, with each material at different dosage levels (6 and 0.6 microg/mL for MWCNO and 0.6 and 0.06 microg/mL for MWCNT). MWCNO and MWCNT exposure activates genes involved in cellular transport, metabolism, cell cycle regulation, and stress response. MWCNTs induce genes indicative of a strong immune and inflammatory response within skin fibroblasts, while MWCNO changes are concentrated in genes induced in response to external stimuli. Promoter analysis of the microarray results demonstrate that interferon and p38/ERK-MAPK cascades are critical pathway components in the induced signal transduction contributing to the more adverse effects observed upon exposure to MWCNTs as compared to MWCNOs.
This paper references
Direct observation of the tetrahedral bonding in graphitized carbon black by high resolution electron microscopy
S. Iijima (1980)
Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine.
F. Dolbeare (1983)
Interferon-induced 56,000 Mr protein and its mRNA in human cells: molecular cloning and partial sequence of the cDNA.
J. Chebath (1983)
γ-Interferon transcriptionally regulates an early-response gene containing homology to platelet proteins
A. Luster (1985)
Interferon-inducible gene maps to a chromosomal band associated with a (4;11) translocation in acute leukemia cells.
A. Luster (1987)
cDNA cloning and assignment to chromosome 21 ofIFI-78K gene, the human equivalent of murineMx gene
M. Horisberger (1988)
Interferon response element of the human gene 6‐16.
A. Porter (1988)
Interferon response element of the human gene 1988 , 6 - 16
A. C. Porter (1988)
cDNA cloning and assignment to chromosome 21 of IFI - 78 K gene , the human equivalent of murine Mx gene
M. A. Horisberger (1988)
Association of IDDM and Attenuated Response of 2′,5′-Oligoadenylate Synthetase to Yellow Fever Vaccine
V. Bonnevie-Nielsen (1989)
Chromosomal localization of two human genes inducible by interferons, double-stranded RNA, and viruses.
I. M. Clauss (1990)
Absence of the type I IFN system in EC cells: Transcriptional activator (IRF-1) and repressor (IRF-2) genes are developmentally regulated
H. Harada (1990)
Repression of c-fos transcription and an altered genetic program in senescent human fibroblasts.
T. Seshadri (1990)
Helical microtubules of graphitic carbon
S. Iijima (1991)
The interferon- and virus-inducible IFI-56K and IFI-54K genes are located on human chromosome 10 at bands q23-q24.
M. Lafage (1992)
SNAP family of NSF attachment proteins includes a brain-specific isoform
S. Whiteheart (1993)
bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death
L. Boise (1993)
Single-shell carbon nanotubes of 1-nm diameter
S. Iijima (1993)
Effects of Acute and Subchronic Exposure of Topically Applied Fullerene Extracts on the Mouse Skin
M. Nelson (1993)
Transient activation of RAF-1, MEK, and ERK2 coincides kinetically with ternary complex factor phosphorylation and immediate-early gene promoter activity in vivo.
R. Hipskind (1994)
Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins.
J. Darnell (1994)
Functional divergence of the MAP kinase pathway ERK1 and ERK2 activate specific transcription factors
C. F. Chuang (1994)
The interferon-inducible, double-stranded RNA-specific adenosine deaminase gene (DSRAD) maps to human chromosome 1q21.1-21.2.
H. Weier (1995)
Onion-Like Graphitic Particles
D. Ugarte (1995)
M. Dresselhaus (1995)
18) Ugarte, D. Onion-like graphitic particles
Science of fullerenes and carbon nanotubes
L. B. Ebert (1996)
Human MxB Protein, an Interferon-α-inducible GTPase, Contains a Nuclear Targeting Signal and Is Localized in the Heterochromatin Region beneath the Nuclear Envelope*
Krister Melén (1996)
The CRKL Adaptor Protein Transforms Fibroblasts and Functions in Transformation by the BCR-ABL Oncogene*
K. Senechal (1996)
Identification of a Novel Syntaxin- and Synaptobrevin/VAMP-binding Protein, SNAP-23, Expressed in Non-neuronal Tissues*
V. Ravichandran (1996)
Eklund, P. C. Science of Fullerenes and Carbon Nanotubes
Individual single-wall carbon nanotubes as quantum wires
S. Tans (1997)
A novel interferon-inducible gene expressed during myeloid differentiation.
T. Niikura (1997)
Regulated secretion in platelets: identification of elements of the platelet exocytosis machinery.
P. P. Lemons (1997)
TRAIL‐R2: a novel apoptosis‐mediating receptor for TRAIL
H. Walczak (1997)
Use of differential display analysis to assess the effect of human cytomegalovirus infection on the accumulation of cellular RNAs: induction of interferon-responsive RNAs.
H. Zhu (1997)
CCAAT/Enhancer-binding Protein-δ (C/EBP-δ) Is Induced in Growth-arrested Mouse Mammary Epithelial Cells*
J. O’Rourke (1997)
cDNA cloning and assignment to chromosome 21 of IFI-78K gene, the human equivalent of murine
A novel interferon-inducible gene
T. Niikura (1997)
Growth Hormone Stimulates Phosphorylation and Activation of Elk-1 and Expression of c-fos, egr-1, andjunB through Activation of Extracellular Signal-regulated Kinases 1 and 2*
C. Hodge (1998)
Stress-induced immediate-early gene, egr-1, involves activation of p38/JNK1
C. P. Lim (1998)
RIP2 Is a Novel NF-κB-activating and Cell Death-inducing Kinase*
J. McCarthy (1998)
Human GBF1 is a ubiquitously expressed gene of the sec7 domain family mapping to 10q24.
S J Mansour (1998)
RIP2 is a novel NF-kappaB-activating and cell death-inducing kinase.
J. McCarthy (1998)
Two distinct cytokines released from a human aminoacyl-tRNA synthetase.
K. Wakasugi (1999)
The role of p38 mitogen-activated protein kinase in IL-1 beta transcription.
J. Baldassare (1999)
Fullerene nanobutes for molecular electronics
D. Colbert (1999)
Activation of the EGF receptor signaling pathway in human airway epithelial cells exposed to metals.
W. Wu (1999)
Nanotubes from Carbon.
P. Ajayan (1999)
Fullerene nanobutes for molecular electronics Quantum electronics: Nanotubes go ballistic
D T Colbert (1999)
Fullerenes : chemistry, physics, and technology
K. Kadish (2000)
The EGF Receptor Provides an Essential Survival Signal for SOS-Dependent Skin Tumor Development
M. Sibilia (2000)
Aromatic hydrocarbon receptor-driven Bax gene expression is required for premature ovarian failure caused by biohazardous environmental chemicals
T. Matikainen (2001)
Carbon Nanotubes: Synthesis, Properties, and Applications
S. Sinnott (2001)
How the news that we were not the first to conceive of soccer ball C60 got to us.
R. F. Curl (2001)
Quantum electronics: Nanotubes go ballistic
C. T. White (2001)
The Transcription Factor GATA4 Is Activated by Extracellular Signal-Regulated Kinase 1- and 2-Mediated Phosphorylation of Serine 105 in Cardiomyocytes
Qiangrong Liang (2001)
Nanotechnology: Synthesis of carbon 'onions' in water
Noboru Sano (2001)
The Usf‐1 transcription factor is a novel target for the stress‐responsive p38 kinase and mediates UV‐induced Tyrosinase expression
Marie-Dominique Galibert (2001)
Kokai Tokkyo Koho 13 pp. ((Sony Corp.: Japan). Jp
H Kajiura (2001)
Properties of carbon onions produced by an arc discharge in water
N. Sano (2002)
Distinct Roles of Mitogen-activated Protein Kinase Pathways in GATA-4 Transcription Factor-mediated Regulation of B-type Natriuretic Peptide Gene*
R. Kerkelä (2002)
Photo-induced cytotoxicity of malonic acid [C(60)]fullerene derivatives and its mechanism.
X. Yang (2002)
Cytotoxicity and photocytotoxicity of a dendritic C(60) mono-adduct and a malonic acid C(60) tris-adduct on Jurkat cells.
F. Rancan (2002)
Two-color, fluorescence-based microplate assay for apoptosis detection.
R. Wronski (2002)
Characterization of carbon nano-onions by using flow field-flow fractionation. Proc. Carbon ′02, Int. Conf. Carbon [ISBN 7-900362- 03-7]
B Chen (2002)
Characterization of carbon nano - onions by using flow field - flow fractionation
B. Chen (2002)
Role of Raf in Vascular Protection from Distinct Apoptotic Stimuli
Alireza Alavi (2003)
Nanocarbon production by arc discharge in water
H. Lange (2003)
Nanomaterials Show Signs of Toxicity
R. Service (2003)
Exposure to Carbon Nanotube Material: Assessment of Nanotube Cytotoxicity using Human Keratinocyte Cells
A. Shvedova (2003)
Multiwall Carbon Nanotubes: Synthesis and Application
R. Andrews (2003)
The potential environmental impact of engineered nanomaterials
V. Colvin (2003)
New Synthesis of High-Quality Double-Walled Carbon Nanotubes by High-Temperature Pulsed Arc Discharge
Toshiki Sugai (2003)
Development and maintenance of B and T lymphocytes requires antiapoptotic MCL-1
J. Opferman (2003)
Modulation of oestrogen receptor signalling by association with the activated dioxin receptor
F. Ohtake (2003)
American Chemical Society meeting . Nanomaterials show signs of toxicity
R. F. Service (2003)
Pulmonary toxicity of single-wall carbon nanotubes in mice 7 and 90 days after intratracheal instillation.
C. Lam (2004)
Nanotechnology. Yellow light for nanotech.
Fiona M. Proffitt (2004)
Effects of exposure to ultrafine carbon particles in healthy subjects and subjects with asthma.
M. Frampton (2004)
Nanometer size diesel exhaust particles are selectively toxic to dopaminergic neurons: the role of microglia, phagocytosis, and NADPH oxidase
M. Block (2004)
A biologically effective fullerene (C60) derivative with superoxide dismutase mimetic properties.
Sameh S. Ali (2004)
Nanoparticle Surface Charges Alter Blood–Brain Barrier Integrity and Permeability
P. Lockman (2004)
Yellow Light for Nanotech
Fiona M. Proffitt (2004)
Manufactured Nanomaterials (Fullerenes, C60) Induce Oxidative Stress in the Brain of Juvenile Largemouth Bass
E. Oberdörster (2004)
Catalytic system of the reactive oxygen species on the C60 fullerene basis.
A. Burlaka (2004)
Toxicity of gold nanoparticles functionalized with cationic and anionic side chains.
Catherine M Goodman (2004)
Exposure to Carbon Nanotube Material: Aerosol Release During the Handling of Unrefined Single-Walled Carbon Nanotube Material
A. Maynard (2004)
Nanotechnology Grows Up
R. Service (2004)
Comparative pulmonary toxicity assessment of single-wall carbon nanotubes in rats.
D. Warheit (2004)
The Differential Cytotoxicity of Water-Soluble Fullerenes
C. Sayes (2004)
Cytotoxicity and photocytotoxicity of a dendritic C(60) monoadduct and a malonic acid
F Rancan (2004)
Multi-walled carbon nanotube interactions with human epidermal keratinocytes.
N. Monteiro-Riviere (2005)
Recombinant adeno-associated virus type 2-mediated gene transfer into human keratinocytes is influenced by both the ubiquitin/proteasome pathway and epidermal growth factor receptor tyrosine kinase
M. Braun-Falco (2005)
Substituted carborane-appended water-soluble single-wall carbon nanotubes: new approach to boron neutron capture therapy drug delivery.
Zhu Yinghuai (2005)
Cytotoxicity of carbon nanomaterials: single-wall nanotube, multi-wall nanotube, and fullerene.
G. Jia (2005)
Cytotoxicity of colloidal CdSe and CdSe/ZnS nanoparticles.
C. Kirchner (2005)
Variation in antiviral 2',5'-oligoadenylate synthetase (2'5'AS) enzyme activity is controlled by a single-nucleotide polymorphism at a splice-acceptor site in the OAS1 gene.
V. Bonnevie-Nielsen (2005)
Gene Expression Profiles of Normal Human Fibroblasts after Exposure to Ionizing Radiation: A Comparative Study of Low and High Doses
Lianghao Ding (2005)
Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer cell destruction.
N. W. Kam (2005)
IRF-7 is the master regulator of type-I interferon-dependent immune responses
K. Honda (2005)
The rat ear vein model for investigating in vivo thrombogenicity of ultrafine particles (UFP).
V. Silva (2005)
Investigating the toxicity of nanoscale materials by gene expression profiling: A systems biology approach
M. J. Cunningham (2005)
This paper is referenced by
Toxicological Perspectives and Environmental Risks of Consumer Nanoproducts
S. Gulati (2021)
Carbon-based heterogeneous photocatalysts for water cleaning technologies: a review
Joana L. Lopes (2021)
Chitosan-Based Nanoparticles of Targeted Drug Delivery System in Breast Cancer Treatment
Yedi Herdiana (2021)
Potential risk and safety concerns of industrial nanomaterials in environmental management
Saurabh N. Joglekar (2021)
Investigating the Molecular Processes behind the Cell-Specific Toxicity Response to Titanium Dioxide Nanobelts
Laurent A Winckers (2021)
Ablation of cells in mice using antibody-functionalized multiwalled carbon nanotubes (Ab-MWCNTs) in combination with microwaves
Amy Chall (2021)
Therapeutic nanostructures and nanotoxicity
A. Sarma (2021)
Nanomaterials and Human Health
S. Tekmen (2021)
Functionalized carbon nano onion as a novel drug delivery system for brain targeting
Rabindranath Majumder (2021)
Pollution assessment of nanomaterials
A. Mohamed (2021)
Nanotoxicity, Cytotoxicity, and Genotoxicity Mechanisms of Nanomaterials
Loutfy H. Madkour (2020)
Toxic and beneficial effects of carbon nanomaterials on human and animal health
Atef A. Hassan (2020)
Application of carbon nano onions in the biomedical field: recent advances and challenges.
J. Ahlawat (2020)
An insight into obtaining of non-spherical particles by mechanical stretching of micro- and nanospheres
Sonia Guha (2020)
Formation of large area closely packed carbon onions film by plasma-based ion implantation
N. Matsumoto (2020)
Carbon nanomaterials against pathogens; the antimicrobial activity of carbon nanotubes, graphene/graphene oxide, fullerenes, and their nanocomposites.
Maryam Azizi-Lalabadi (2020)
Multi-walled carbon nanotubes induce oxidative stress, apoptosis, and dysfunction in isolated rat heart mitochondria: protective effect of naringin
M. Salehcheh (2020)
Enabling highly effective underwater oxygen-consuming reaction at solid-liquid-air triphasic interface
J. Bai (2020)
Nanomaterials and nanocomposite applications in veterinary medicine
Atef A. Hassan (2020)
Cellular Toxicity Study of Silicon Nanowires
Ziyan Song (2020)
Programmed cell death mechanisms and nanoparticle toxicity
Loutfy H. Madkour (2020)
Toxicity of carbon nanomaterials
S. Ray (2020)
A review of imperative concerns against clinical translation of nanomaterials: Unwanted biological interactions of nanomaterials cause serious nanotoxicity
Z. Hussain (2020)
Toxic effects of engineered carbon nanoparticles on environment
Sayali S. Patil (2020)
Risk management and regulatory aspects of carbon nanomaterials
Khalid Parwez (2020)
Nanocomposite Films of Chitosan-Grafted Carbon Nano-Onions for Biomedical Applications
Carlos David Grande Tovar (2020)
Metal nanoparticles (MNPs) and particulate matter (PM) induce toxicity
Loutfy H. Madkour (2020)
Toxicity of carbon-based nanomaterials: Reviewing recent reports in medical and biological systems.
Rasoul Madannejad (2019)
Risks of Nanotechnology to Human Life
M. Nasrollahzadeh (2019)
Postsynthetic treatment of carbon nano-onions: Surface modification by heteroatoms to enhance their capacitive and electrocatalytic properties
Olena Butsyk (2019)
Влияние одностенных углеродных нанотрубок на гомеостаз микроэлементов в организме крыс WISTAR в подостром эксперименте продолжительностью 92 суток
И. В. Гмошинский (2019)
Carbon nano-onions as promising nanomaterial for biomedical and electrochemical applications
A. Camisasca (2019)See more