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Gold Nanoparticle Enhanced Electrochemiluminescence Of CdS Thin Films For Ultrasensitive Thrombin Detection.

Jing Wang, Y. Shan, W. Zhao, J. Xu, H. Chen
Published 2011 · Chemistry, Medicine

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Interactions between surface plasmons (SP) of metallic surfaces and photoluminescence (PL) of semiconductor nanocrystal (S-NC) surfaces have been extensively investigated, and SP-induced PL enhancement has been used as a sensitive analytical technique. However, this SP induced electrochemiluminescence (ECL) enhancement is rarely studied. In this work, we report greatly enhanced ECL of CdS thin films by gold nanoparticles (Au NPs) for ultrasensitive detection of thrombin. The system was composed of a CdS NC film on glassy carbon electrode (GCE) as ECL emitter attached an aptamer of thrombin. Then, ssDNA-AuNP conjugates hybridized with the aptamer to form a separation length of ca. 12 nm between CdS NCs and Au NPs. The system showed 5-fold enhancement of ECL intensity as compared to that without Au NPs, which might be attributed to the long-distance interaction between the S-NCs and SPR field of noble metal nanoparticles (MNPs).We also found that the enhanced ECL could be influenced by the involving factors such as the separation distance, spectral overlap, and magnetic field. Such enhancement in combination with smart recognition of aptamer and target protein allowed us to construct an ultrasensitive aptasensor for attomolar detection of thrombin. The presence of target protein was reflected by the ECL signal decrease caused by the target-induced removal of ssDNA-AuNP conjugates. The decrease of ECL signal was logarithmically linear with the concentration of thrombin in a wide range from 100 aM to 100 fM. The principle described in this work could be also applied to many other bioassays.
This paper references
10.1021/NL0257824
Electrogenerated Chemiluminescence of CdSe Nanocrystals
N. Myung (2002)
10.1021/AC0495236
Electrogenerated chemiluminescence. 77. DNA hybridization detection at high amplification with [Ru(bpy)3]2+-containing microspheres.
W. Miao (2004)
10.1021/AC048782S
Electrogenerated chemiluminescence. 80. C-reactive protein determination at high amplification with [Ru(bpy)3]2+-containing microspheres.
W. Miao (2004)
10.1021/NL049516X
Electrochemistry and electrogenerated chemiluminescence of CdTe nanoparticles
Yoonjung Bae (2004)
10.1016/j.bios.2009.08.008
DNA aptamer-based QDs electrochemiluminescence biosensor for the detection of thrombin.
Haiping Huang (2009)
10.1016/j.talanta.2009.10.036
An aptamer-based assay for thrombin via structure switch based on gold nanoparticles and magnetic nanoparticles.
J. Zheng (2010)
10.1021/JA0296529
Simultaneous direct electrochemiluminescence and catalytic voltammetry detection of DNA in ultrathin films.
L. Dennany (2003)
10.1038/nnano.2009.193
Plasmonic fluorescent quantum dots.
Yongdong Jin (2009)
10.1021/CM0492336
Seed-Mediated Synthesis of Gold Nanorods: Role of the Size and Nature of the Seed
Anand Gole and (2004)
10.1364/OE.18.006516
Plasmon-enhanced Förster energy transfer between semiconductor quantum dots: multipole effects.
Xiong-Rui Su (2010)
10.1016/j.bios.2010.06.044
Nanomaterial-amplified "signal off/on" electrogenerated chemiluminescence aptasensors for the detection of thrombin.
Y. Li (2010)
10.1007/S11468-007-9031-1
Review of Some Interesting Surface Plasmon Resonance-enhanced Properties of Noble Metal Nanoparticles and Their Applications to Biosystems
P. Jain (2007)
10.1063/1.2388870
Fluorescence enhancement of quantum dots enclosed in Au nanopockets with subwavelength aperture
G. Liu (2006)
10.1103/PHYSREVLETT.96.113002
Enhancement and quenching of single-molecule fluorescence.
P. Anger (2006)
10.1021/NL025819K
Enhanced Luminescence of CdSe Quantum Dots on Gold Colloids
O. Kulakovich (2002)
10.1016/J.ELECOM.2009.04.004
Anodic electrochemiluminescence of CdSe nanoparticles coreacted with tertiary amine and halide induced quenching effect
H. Jiang (2009)
10.1063/1.2937142
Photoluminescence enhancement and quenching of single CdSe∕ZnS nanocrystals on metal surfaces dominated by plasmon resonant energy transfer
K. Matsuda (2008)
10.1016/J.TSF.2009.10.166
Localized surface plasmon resonance enhanced photoluminescence of CdSe QDs in PMMA matrix on silver colloids with different shapes
L. Lu (2010)
10.1021/NL0602140
Exciton-plasmon interaction and hybrid excitons in semiconductor-metal nanoparticle assemblies
A. Govorov (2006)
10.1007/s00216-008-2185-7
Semiconductor quantum dots and metal nanoparticles: syntheses, optical properties, and biological applications
V. Biju (2008)
10.1016/j.bios.2009.04.016
An aptamer-based electrochemiluminescent biosensor for ATP detection.
W. Yao (2009)
10.1021/ac901609g
Label-free electrochemiluminescent aptasensor with attomolar mass detection limits based on a Ru(phen)(3)(2+)-double-strand DNA composite film electrode.
Xue-Bo Yin (2009)
10.1021/JA0379215
Probing photoelectrochemical processes in Au-CdS nanoparticle arrays by surface plasmon resonance: application for the detection of acetylcholine esterase inhibitors.
M. Zayats (2003)
10.1002/adma.200903745
Two-photon excited fluorescence enhancement for ultrasensitive DNA detection on large-area gold nanopatterns.
Jin-Mi Jung (2010)
10.1021/AC9712310
Enhancement of molecular fluorescence near the surface of colloidal metal films.
K. Sokolov (1998)
10.1021/nl803459b
Electrogenerated chemiluminescence from PbS quantum dots.
L. Sun (2009)
10.1039/b821049g
Distance-dependent quenching and enhancing of electrochemiluminescence from a CdS:Mn nanocrystal film by Au nanoparticles for highly sensitive detection of DNA.
Y. Shan (2009)
10.1039/c0cc00660b
Photoluminescence enhancement in CdSe/ZnS-DNA linked-Au nanoparticle heterodimers probed by single molecule spectroscopy.
M. Maye (2010)
10.1006/JMBI.1997.1275
Oligonucleotide inhibitors of human thrombin that bind distinct epitopes.
D. M. Tasset (1997)
10.1002/1097-0282(2000)57:6<325::AID-BIP10>3.0.CO;2-7
Selective enhancement of Raman or fluorescence spectra of biomolecules using specifically annealed thick gold films.
N. Strekal (2000)
10.1021/JP908387Y
Highly Sensitive Emission Sensor Based on Surface Plasmon Enhanced Energy Transfer between Gold Nanoclusters and Silver Nanoparticles
Chihwei Chen (2010)
10.1002/1616-3028(20020916)12:9<575::AID-ADFM575>3.0.CO;2-4
Surface-plasmon fluorescence spectroscopy
T. Neumann (2002)
10.1016/J.ACA.2008.08.041
A electrochemiluminescence aptasensor for detection of thrombin incorporating the capture aptamer labeled with gold nanoparticles immobilized onto the thio-silanized ITO electrode
L. Fang (2008)
10.1016/j.bios.2009.04.019
A solid-state electrochemiluminescence biosensing switch for detection of thrombin based on ferrocene-labeled molecular beacon aptamer.
X. Wang (2009)
10.1103/PHYSREVLETT.89.117401
Surface-enhanced emission from single semiconductor nanocrystals.
K. Shimizu (2002)
10.1021/ac8017197
Electrochemical DNA biosensor based on nanoporous gold electrode and multifunctional encoded DNA-Au bio bar codes.
Kongcheng Hu (2008)
10.1016/J.ELECOM.2007.07.038
Electrogenerated chemiluminescence aptamer-based biosensor for the determination of cocaine
Y. Li (2007)
10.1016/S0927-7757(99)00550-6
Surface-Plasmon Field-Enhanced Fluorescence Spectroscopy
Thorsten Liebermann (2000)
10.1021/AC062357C
CdS nanocrystal-based electrochemiluminescence biosensor for the detection of low-density lipoprotein by increasing sensitivity with gold nanoparticle amplification.
G. Jie (2007)



This paper is referenced by
10.1016/B978-0-444-63285-2.00011-0
Applications of Gold Nanoparticles in Electroanalysis
M. Trojanowicz (2014)
10.1007/s00604-020-04453-x
An “off-on” electrochemiluminescence aptasensor for microcystin-LR assay based on the resonance energy transfer from PTCA/NH2-MIL-125(Ti) to gold nanoparticles
J. Li (2020)
10.1016/J.JELECHEM.2012.11.017
Cathodic electrochemiluminescence of Eosin Y–peroxydisulfate system and its analytical application for determination of guanine
Y. Zhu (2013)
10.1021/ac501532y
In situ activation of CdS electrochemiluminescence film and its application in H₂S detection.
Y. Zhang (2014)
10.1021/ac501120y
Electrochemical signal amplification for immunosensor based on 3D interdigitated array electrodes.
D. Han (2014)
10.1016/J.TALANTA.2020.121674
Plasmon-enhanced quantum dots electrochemiluminescence aptasensor for selective and sensitive detection of cardiac troponin I.
Shimeles Addisu Kitte (2021)
10.1016/S1872-2040(15)60877-5
A TiO2/CNTs Nanocomposites Enhanced Luminol Electrochemiluminescence Assay for Glucose Detection
Yong-Hong Wang (2015)
10.1021/acs.analchem.6b00012
Protein Binding Bends the Gold Nanoparticle Capped DNA Sequence: Toward Novel Energy-Transfer-Based Photoelectrochemical Protein Detection.
Zhengyuan Ma (2016)
10.1039/c3cc42637h
Highly sensitive electrochemiluminescent cytosensing using carbon nanodot@Ag hybrid material and graphene for dual signal amplification.
L. Wu (2013)
10.1021/acs.analchem.7b01498
Boolean Logic Tree of Label-Free Dual-Signal Electrochemical Aptasensor System for Biosensing, Three-State Logic Computation, and Keypad Lock Security Operation.
J. Lu (2017)
10.1002/anie.201508801
Electrochemiluminescent Spin-Polarized Modulation by Magnetic Ions and Surface Plasmon Coupling.
Y. Shan (2016)
10.1134/S003602441402006X
Synthesis and electrophoretic concentration of nanoparticles of CdS in reversed micellar solutions
A. I. Bulavchenko (2014)
10.1016/j.trac.2019.115746
Recent advances in electrochemiluminescence resonance energy transfer for bioanalysis: Fundamentals and applications
Hai-Jie Lu (2020)
10.1021/am506980d
Biobar-coded gold nanoparticles and DNAzyme-based dual signal amplification strategy for ultrasensitive detection of protein by electrochemiluminescence.
H. Xia (2015)
10.1021/ac203261x
Novel magnetic Fe3O4@CdSe composite quantum dot-based electrochemiluminescence detection of thrombin by a multiple DNA cycle amplification strategy.
G. Jie (2012)
10.1039/C8GC03010C
A visual electrochemiluminescence resonance energy transfer/surface plasmon coupled electrochemiluminescence nanosensor for Shiga toxin-producing Escherichia coli detection
Y. Liu (2018)
10.1016/J.TRAC.2018.12.022
Water-dispersed luminescent quantum dots for miRNA detection
O. A. Goryacheva (2019)
10.1007/s00216-020-02440-y
A visual electrochemiluminescence biosensor based on CuInZnS quantum dots for superoxide dismutase detection
Yang Liu (2020)
10.1007/s00604-017-2177-4
An electrochemiluminescence aptasensor switch for aldicarb recognition via ruthenium complex-modified dendrimers on multiwalled carbon nanotubes
Shuhuai Li (2017)
10.1016/J.APSUSC.2015.12.037
Electrochemiluminescent detection of Pb2+ by graphene/gold nanoparticles and CdSe quantum dots
L. Lu (2016)
10.1021/ACS.BIOCONJCHEM.6B00553
Conformational Changes of Enzymes and Aptamers Immobilized on Electrodes.
A. Tello (2016)
10.1007/s00604-011-0758-1
Electrochemical biosensing based on noble metal nanoparticles
J. Wang (2012)
10.1016/j.bios.2017.11.034
Switchable electrochemiluminescence aptasensor coupled with resonance energy transfer for selective attomolar detection of Hg2+ via CdTe@CdS/dendrimer probe and Au nanoparticle quencher.
B. Babamiri (2018)
10.1016/j.bios.2014.11.011
Silver nanowires-based signal amplification for CdSe quantum dots electrochemiluminescence immunoassay.
Tingyu Huang (2015)
10.1039/c2cc17997k
An aptamer based on-plate microarray for high-throughput insulin detection by MALDI-TOF MS.
Xueyang Zhang (2012)
10.1016/j.bios.2014.03.012
An off-on-off electrochemiluminescence approach for ultrasensitive detection of thrombin.
Li Deng (2014)
10.1134/S1995078019040116
Application of Gold Nanoparticles for High-Sensitivity Fluorescence Polarization Aptamer Assay for Ochratoxin A
A. V. Samokhvalov (2019)
10.1039/c3cc49719d
Electrochemiluminescence signal amplification combined with a conformation-switched hairpin DNA probe for determining the methylation level and position in the Hsp53 tumor suppressor gene.
Hui Bin Zhang (2014)
10.15406/PPIJ.2018.06.00172
Applications of gold nanoparticles in medicine and therapy
Loutfy H. Madkour (2018)
10.1039/C2AY25878A
Electrochemiluminescence sensor using gold-nanoparticle modification combining mercaptoacetic acid-assembly
C. Ma (2012)
10.1007/s00604-014-1322-6
Detection of DNA damage by exploiting the distance dependence of the electrochemiluminescence energy transfer between quantum dots and gold nanoparticles
L. Lu (2014)
10.1039/C7TC05474B
Recent advances in quantum dot-based electrochemiluminescence sensors
X. Chen (2018)
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