Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Dextran-coated Gold Nanoparticles For The Assessment Of Antimicrobial Susceptibility.

S. Nath, C. Kaittanis, A. Tinkham, J. M. Pérez
Published 2008 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Bacteria rapidly evolve mechanisms to become resistant to antibiotics. Therefore, identifying an effective antibiotic or antibacterial agent and administering it at concentrations that will successfully prevent bacterial growth (antimicrobial susceptibility) is critical for health care decision making and vital for the battle against multi-drug-resistant bacteria. Currently, the determination of antimicrobial susceptibility requires at least 24 h. Herein, we describe a nanoparticle-based antimicrobial susceptibility assay based on the concanavalin A-induced clustering of dextran-coated gold nanoparticles, which sense the presence of available complex carbohydrates in bacterial suspension. Under conditions of bacterial growth inhibition, addition of concanavalin A results in the formation of extensive dextran gold nanoassemblies, which are facilitated by the presence of free carbohydrates in solution and result in large shifts in the surface plasmon band of the nanoparticles. Meanwhile, at conditions of increased bacterial growth, a decrease in the amount of free carbohydrates in solution will occur due to an increased carbohydrate uptake by the proliferating bacteria. This will result in a decrease in the size of the gold nanoparticle clusters and an increase in the number of nanoparticles that bind to bacterial surface carbohydrates, causing lower shifts in the plasmonic band. The gold nanoparticle-based assessment of antimicrobial susceptibility yields results within 3 h and can be used for the high-throughput screening of samples during epidemics and identification of potential antimicrobial agents to expedite clinical decision-making in point-of-care diagnostics.

This paper is referenced by
Glycans in nanomedicine, impact and perspectives.
Susanna Sampaolesi (2019)
Cerium oxide nanoparticles for the detection of antimicrobial resistance 2011
A. Noll (2018)
Polymers-Metal Nanocomposites
Theivasanthi Thirugnanasambandan (2019)
Cytotoxicity evaluation and antimicrobial studies of starch capped water soluble copper nanoparticles.
Mayur Valodkar (2012)
Facile and eco-benign synthesis of Au@Fe2O3 nanocomposite: Efficient photocatalytic, antibacterial and antioxidant agent.
S. Shams (2019)
Synthesis of Gold Nanoparticles Stabilized in Dextran Solution by Gamma Co-60 Ray Irradiation and Preparation of Gold Nanoparticles/Dextran Powder
Phan Hà Nữ Diễm (2017)
Synthesis of Colloidal Au Nanoparticles through Ultrasonic Spray Pyrolysis and Their Use in the Preparation of Polyacrylate-AuNPs’ Composites
D. Golub (2019)
Sonochemical synthesis and application of rhodium–graphene nanocomposite
Sourov Chandra (2011)
Analyzing Carbohydrate-Protein Interaction Based on Single Plasmonic Nanoparticle by Conventional Dark Field Microscopy.
Hong-Ying Jin (2015)
Detection of glucose in synthetic tear fluid using dually functionalized gold nanoparticles.
S. Manju (2011)
Coating matters: the influence of coating materials on the optical properties of gold nanoparticles
M. Chanana (2012)
Nanotools for Sepsis Diagnosis and Treatment.
Lana Papafilippou (2020)
Dextran-encapsulated photoluminescent gold nanoclusters: synthesis and application
Wei-Ju Chiu (2014)
Cerium oxide nanoparticles for the detection of antimicrobial resistance
A. Noll (2011)
Thioglucose-stabilized gold nanoparticles as a novel platform for colorimetric bioassay based on nanoparticle aggregation.
S. Watanabe (2010)
Biosensors in Antimicrobial Drug Discovery: Since Biology until ScreeningPlatforms
J. Bueno (2014)
A one-pot strategy for biomimetic synthesis and self-assembly of gold nanoparticles.
Yi Wang (2010)
Dissecting microbiological systems using materials science.
A. Muralimohan (2009)
Polymer thin film with in situ synthesized silver nanoparticles as a potent reusable bactericide
S. Poree (2011)
Cell membrane coated nanocarriers - an efficient biomimetic platform for targeted therapy.
Pratigyan Dash (2020)
Screening of antibiotic susceptibility to β-lactam-induced elongation of Gram-negative bacteria based on dielectrophoresis.
Cheng-Che Chung (2012)
Rapid, simple, and high-throughput antimicrobial susceptibility testing and antibiotics screening.
Chunlei Zhu (2011)
Graphene oxide and dextran capped gold nanoparticles based surface plasmon resonance sensor for sensitive detection of concanavalin A.
Chun-Fang Huang (2013)
Design of Photosensitizing Agents for Targeted Antimicrobial Photodynamic Therapy
Maxime Klausen (2020)
Biological response of hydrogels embedding gold nanoparticles.
E. Marsich (2011)
Cutting-Edge Technologies for Detection of Plant Viruses in Vegetatively Propagated Crop Plants
R. Selvarajan (2016)
Nanomedicine in the Management of Microbial Infection - Overview and Perspectives.
Xi Zhu (2014)
Sandwich-configuration electrochemiluminescence biosensor based on Ag nanocubes–polyamidoamine dendrimer–luminol nanocomposite for Con A detection
X. Ou (2016)
Magnetic Nanosensors For Multiplexed Bacterial Pathogenesis Identification
Charalambos Kaittanis (2010)
Nanomaterial-based sensors for detection of foodborne bacterial pathogens and toxins as well as pork adulteration in meat products.
B. Stephen Inbaraj (2016)
Photoinactivation of multidrug resistant bacteria by monomeric methylene blue conjugated gold nanoparticles.
Shakir Khan (2017)
Rapid Nanoparticle-Mediated Monitoring of Bacterial Metabolic Activity and Assessment of Antimicrobial Susceptibility in Blood with Magnetic Relaxation
C. Kaittanis (2008)
See more
Semantic Scholar Logo Some data provided by SemanticScholar