Online citations, reference lists, and bibliographies.
← Back to Search

Gold Nanoparticles Doped Conducting Polymer Nanorod Electrodes: Ferrocene Catalyzed Aptamer-based Thrombin Immunosensor.

M. Rahman, J. Son, M. Won, Y. Shim
Published 2009 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
Au nanoparticles-doped conducting polymer nanorods electrodes (AuNPs/CPNEs) were prepared by coating Au nanorods (AuNRs) with a conducting polymer layer. The AuNRs were prepared through an electroless deposition method using the polycarbonate membrane (pore diameter, 50 nm, pore density, 6 x 10(8) pores/cm(2)) as a template. The AuNPs/CPNEs combining catalytic activity of ferrocene to ascorbic acid were used for the fabrication of an ultrasensitive aptamer sensor for thrombin detection. The AuNPs/3D-CPNEs were characterized employing cyclic voltammetry (CV), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and atomic force microscopy (AFM). Sandwiched immunoassay for alpha-human thrombin with NH(2)-functionalized-thrombin binding aptamer (Apt) immobilized on AuNPs/3D-CPNEs was studied through the electrocatalytic oxidation of ascorbic acid by the ferrocene moiety that was bound with an antithrombin antibody and attached with the Apt/3D-CPNEs probe through target binding. Various experimental parameters affecting thrombin detection were optimized, and the performance of the thrombin aptamer sensor was examined. The Apt/AuNPs/3D-CPNEs based thrombin sensor exhibited a wide dynamic range of 5-2000 ng L(-1) and a low detection limit of 5 ng L(-1) (0.14 pM). The selectivity and the stability of the proposed thrombin aptamer sensor were excellent, and it was tested in a real human serum sample for the detection of spiked concentrations of thrombin.
This paper references



This paper is referenced by
10.1016/J.APSADV.2021.100064
Nanomaterials based electrochemical nucleic acid biosensors for environmental monitoring: A review
A. Hashem (2021)
10.1039/d0ay00678e
The application of antibody-aptamer hybrid biosensors in clinical diagnostics and environmental analysis.
M. Jarczewska (2020)
10.1109/JLT.2018.2878762
Label-Free Thrombin Detection Using a Tapered Fiber-Optic Interferometric Aptasensor
D. Sun (2019)
10.1007/978-3-319-69378-1_9
CNT Applications in Sensors and Actuators
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_32
Structural Aspects and Morphology of CPs
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_3
Synthesis, Purification, and Chemical Modification of CNTs
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_20
Graphene Applications in Sensors
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_37
Batteries and Energy Devices
P. Chandrasekhar (2018)
10.1155/2018/5307106
Electrochemical and AFM Characterization of G-Quadruplex Electrochemical Biosensors and Applications
A. Chiorcea-Paquim (2018)
10.1007/978-3-319-69378-1_21
Graphene Applications in Batteries and Energy Devices
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_10
CNT Applications in Drug and Biomolecule Delivery
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_24
Medical and Pharmaceutical Applications of Graphene
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_11
CNT Applications in Microelectronics, “Nanoelectronics,” and “Nanobioelectronics”
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_4
Physical, Mechanical, and Thermal Properties of CNTs
P. Chandrasekhar (2018)
Nanoscale Characterization and Mechanism of Electroless Deposition of Silver Metal
C. Grabill (2018)
10.3390/s18020589
Detection of Thrombin Based on Fluorescence Energy Transfer between Semiconducting Polymer Dots and BHQ-Labelled Aptamers
Yizhang Liu (2018)
10.1007/978-3-319-69378-1_25
Graphene Applications in Specialized Materials
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_42
Electrochemomechanical, Chemomechanical, and Related Devices
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_22
Graphene Applications in Electronics, Electrical Conductors, and Related Uses
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_8
CNT Applications in Batteries and Energy Devices
P. Chandrasekhar (2018)
10.1016/j.bios.2018.04.024
Multiplexed aptasensor based on metal ions labels for simultaneous detection of multiple antibiotic residues in milk.
Falan Li (2018)
10.1007/978-3-319-69378-1_14
CNT Applications in the Environment and in Materials Used in Separation Science
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_15
Miscellaneous CNT Applications
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_27
Introducing Conducting Polymers (CPs)
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_31
Syntheses and Processing of CPs
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_39
Displays, Including Light-Emitting Diodes (LEDs) and Conductive Films
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_13
CNT Applications in Electrical Conductors, “Quantum Nanowires,” and Potential Superconductors
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_5
Toxicology of CNTs
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_26
Miscellaneous Applications of Graphene
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_12
Graphene Applications in Displays and Transparent, Conductive Films/Substrates
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_30
Basic Electrochemistry of CPs
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_19
Brief, General Overview of Applications
P. Chandrasekhar (2018)
See more
Semantic Scholar Logo Some data provided by SemanticScholar