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The Promising Nanocarrier For Doxorubicin And SiRNA Co-delivery By PDMAEMA-based Amphiphilic Nanomicelles.
Qiang Cheng, Lili Du, Lingwei Meng, Shangcong Han, Tuo Wei, X. Wang, Y. Wu, Xinyun Song, J. Zhou, Shuquan Zheng, Yuanyu Huang, Xing-Jie Liang, H. Cao, Anjie Dong, Zicai Liang
Published 2016 · Medicine, Materials Science
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Synergistic effects of anticancer drug and siRNA have displayed superior advantages for cancer therapy. Herein, we deeply analyzed the feasibility that whether doxorubicin (DOX) and siRNA could be co-delivered by mPEG-PCL-graft-PDMAEMA (PECD) micelles, which mediated excellent DNA/siRNA delivery in vitro and in vivo reported in our previous work. DOX-loaded NPs (PECD-D) were developed by nanoprecipitation technology and exhibited high drug loading content (DLC, 9.5%). In vitro cytotoxicity study in MDA-MB-231 cells, PECD-D treated groups had lower IC50 compared to free DOX groups (F-DOX) at different transfection time (24, 48, and 72h), which maybe attribute to its high cellular uptake and endosomal escape properties. The speculation was confirmed with the results of drug release profile in acidic media, flow cytometry analysis and confocal images. Futhermore, Cy5 labeled siRNA was introduced in PECD-D micelles (PECD-D/siRNA) to track the behavior of dual-loaded nanodrug in vitro and in vivo. Flow cytometry analysis presented that DOX and siRNA were successfully co-delivered into cells, the positive cells ratio were 94.6 and 99.5%, respectively. Confocal images showed that not only DOX and siRNA existed in cytoplasm, but DOX traversed endosome/lysosome and entered into cell nucleus. For in vivo tumor-targeting evaluation in BALB/c nude mice, both DOX and Cy5-siRNA could be detected in tumor sites after intravenous injection with PECD-D/siRNA formulation. Therefore, we believed that PECD micelles have a potential ability as DOX and siRNA co-delivery carrier for cancer therapy.
This paper references
PDMAEMA based gene delivery materials
S. Agarwal (2012)
The role of surface charge in cellular uptake and cytotoxicity of medical nanoparticles
E. Fröhlich (2012)
Co-delivery of doxorubicin and paclitaxel by PEG-polypeptide nanovehicle for the treatment of non-small cell lung cancer.
Shixian Lv (2014)
An approach to prepare polyethylenimine functionalized silica-based spheres with small size for siRNA delivery.
Meihua Yu (2014)
Hyaluronic acid-decorated graphene oxide nanohybrids as nanocarriers for targeted and pH-responsive anticancer drug delivery.
Erqun Song (2014)
Accumulation of sub-100 nm polymeric micelles in poorly permeable tumours depends on size.
H. Cabral (2011)
PLGA nanoparticles containing various anticancer agents and tumour delivery by EPR effect.
Sarbari Acharya (2011)
Engineered breast tumor targeting peptide ligand modified liposomal doxorubicin and the effect of peptide density on anticancer activity.
M. Shahin (2013)
Co-delivery of siRNA and therapeutic agents using nanocarriers to overcome cancer resistance.
M. Creixell (2012)
Knocking down barriers: advances in siRNA delivery
K. Whitehead (2009)
Dual and multi-stimuli responsive polymeric nanoparticles for programmed site-specific drug delivery.
R. Cheng (2013)
Anticancer drug nanomicelles formed by self-assembling amphiphilic dendrimer to combat cancer drug resistance
Tuo Wei (2015)
pH-sensitive nano-systems for drug delivery in cancer therapy.
J. Liu (2014)
Liposomal drug delivery systems: from concept to clinical applications.
T. Allen (2013)
Cardiotoxicity of doxorubicin is mediated through mitochondrial iron accumulation.
Yoshihiko Ichikawa (2014)
Coadministration of a Tumor-Penetrating Peptide Enhances the Efficacy of Cancer Drugs
K. Sugahara (2010)
Nanoparticles for targeted Drug Delivery in Cancer Treatment
Clara Mattu (2010)
Polycation-detachable nanoparticles self-assembled from mPEG-PCL-g-SS-PDMAEMA for in vitro and in vivo siRNA delivery.
Daoshu Lin (2013)
Polycation-siRNA nanoparticles can disassemble at the kidney glomerular basement membrane
Jonathan E Zuckerman (2012)
Folate-decorated poly(lactide-co-glycolide)-vitamin E TPGS nanoparticles for targeted drug delivery.
Z. Zhang (2007)
Translating materials design to the clinic.
J. Hubbell (2013)
Recent advances in delivery of drug-nucleic acid combinations for cancer treatment.
J. Li (2013)
Effects of hydrophobic core components in amphiphilic PDMAEMA nanoparticles on siRNA delivery.
Shangcong Han (2015)
Reversal of multidrug resistance by the inhibition of ATP-binding cassette pumps employing "Generally Recognized As Safe" (GRAS) nanopharmaceuticals: A review.
A. Sosnik (2013)
Multidrug resistance in cancer: role of ATP–dependent transporters
M. Gottesman (2002)
The effect of guanidinylation of PEGylated poly(2-aminoethyl methacrylate) on the systemic delivery of siRNA.
Q. Cheng (2013)
In vivo disassembly of IV administered siRNA matrix nanoparticles at the renal filtration barrier.
B. Naeye (2013)
PLK1shRNA and doxorubicin co-loaded thermosensitive PLGA-PEG-PLGA hydrogels for osteosarcoma treatment.
Hecheng Ma (2014)
Monitoring the expression profiles of doxorubicin-induced and doxorubicin-resistant cancer cells by cDNA microarray.
K. Kudoh (2000)
Star-shaped cyclodextrin-poly(l-lysine) derivative co-delivering docetaxel and MMP-9 siRNA plasmid in cancer therapy.
T. Liu (2014)
Enhanced endosomal/lysosomal escape by distearoyl phosphoethanolamine-polycarboxybetaine lipid for systemic delivery of siRNA.
Y. Li (2014)
Tumor-homing poly-siRNA/glycol chitosan self-cross-linked nanoparticles for systemic siRNA delivery in cancer treatment.
S. Lee (2012)
pH and thermo dual-stimuli-responsive drug carrier based on mesoporous silica nanoparticles encapsulated in a copolymer-lipid bilayer.
X. Wu (2013)
Thermal Targeting of an Acid-Sensitive Doxorubicin Conjugate of Elastin-like Polypeptide Enhances the Therapeutic Efficacy Compared with the Parent Compound In Vivo
Shama Moktan (2012)
Amphiphilic polyelectrolyte/prodrug nanoparticles constructed by synergetic electrostatic and hydrophobic interactions with cooperative pH-sensitivity for controlled doxorubicin delivery.
P. Huang (2015)
Intracellular cleavable poly(2-dimethylaminoethyl methacrylate) functionalized mesoporous silica nanoparticles for efficient siRNA delivery in vitro and in vivo.
Daoshu Lin (2013)
Nanoparticles for Efficient Anticancer Drug Delivery
X. Wu (2011)
Structural contributions of blocked or grafted poly(2-dimethylaminoethyl methacrylate) on PEGylated polycaprolactone nanoparticles in siRNA delivery.
Daoshu Lin (2011)
SiRNA-phospholipid conjugates for gene and drug delivery in cancer treatment.
H. Liu (2014)
Tailor-made dual pH-sensitive polymer-doxorubicin nanoparticles for efficient anticancer drug delivery.
Jin-Zhi Du (2011)
Functionalized nanoscale micelles improve drug delivery for cancer therapy in vitro and in vivo.
Tuo Wei (2013)
The EPR effect: Unique features of tumor blood vessels for drug delivery, factors involved, and limitations and augmentation of the effect.
J. Fang (2011)
Unimolecular Combination of an Atom Transfer Radical Polymerization Initiator and a Lactone Monomer as a Route to New Graft Copolymers
D. Mecerreyes (1999)
G. Hannon (2002)
Folate-decorated hydrophilic three-arm star-block terpolymer as a novel nanovehicle for targeted co-delivery of doxorubicin and Bcl-2 siRNA in breast cancer therapy.
J. Qian (2015)
Non-viral vectors for gene-based therapy
H. Yin (2014)
H-ferritin–nanocaged doxorubicin nanoparticles specifically target and kill tumors with a single-dose injection
Minmin Liang (2014)
Self-assembled RNA interference microsponges for efficient siRNA delivery.
J. Lee (2012)
Novel cationic triblock copolymer of poly[2-(dimethylamino)ethyl methacrylate]-block-poly(β-amino ester)-block-poly[2-(dimethylamino)ethyl methacrylate]: a promising non-viral gene delivery system.
R. Cordeiro (2015)
Intracellular targeting delivery of liposomal drugs to solid tumors based on EPR effects.
K. Maruyama (2011)
Disassemble at the Kidney Glomerular Basement Membrane
B. Naeye (2013)
Efficient Delivery of DOX to Nuclei of Hepatic Carcinoma Cells in the Subcutaneous Tumor Model Using pH-Sensitive Pullulan-DOX Conjugates.
H. Li (2015)
Adaptive amphiphilic dendrimer-based nanoassemblies as robust and versatile siRNA delivery systems.
Xiaoxuan Liu (2014)
Dimeric DNA Aptamer Complexes for High-capacity–targeted Drug Delivery Using pH-sensitive Covalent Linkages
Olcay Boyacioglu (2013)
Ternary complexes of amphiphilic polycaprolactone-graft-poly (N,N-dimethylaminoethyl methacrylate), DNA and polyglutamic acid-graft-poly(ethylene glycol) for gene delivery.
S. Guo (2011)
This paper is referenced by
Recent progress in nanomaterials for gene delivery applications.
Erhan Keleş (2016)
D-α-tocopherol polyethylene glycol 1000 succinate-modified liposomes with an siRNA corona confer enhanced cellular uptake and targeted delivery of doxorubicin via tumor priming
Xi Tan (2019)
In Situ Synthesis of Ultrathin ZIF-8 Film-Coated MSNs for Codelivering Bcl 2 siRNA and Doxorubicin to Enhance Chemotherapeutic Efficacy in Drug-Resistant Cancer Cells.
Qingshan Pan (2018)
Near-infrared triggered co-delivery of doxorubicin and quercetin by using gold nanocages with tetradecanol to maximize anti-tumor effects on MCF-7/ADR cells.
Z. Zhang (2018)
Recent advancements in erythrocytes, platelets, and albumin as delivery systems
P. Xu (2016)
Synergistic Nanoparticle Formulations against Multi-drug Resistant Breast Cancers
Petro Czupiel (2019)
Research and development of drug delivery systems based on drug transporter and nano-formulation
Yi Peng (2020)
Polymeric Co-Delivery Systems in Cancer Treatment: An Overview on Component Drugs’ Dosage Ratio Effect
Jiayi Pan (2019)
Co-Delivery of Drugs and Genes Using Polymeric Nanoparticles for Synergistic Cancer Therapeutic Effects.
Y. Li (2018)
Triptorelin Tethered Multifunctional PAMAM-Histidine-PEG Nanoconstructs Enable Specific Targeting and Efficient Gene Silencing in LHRH Overexpressing Cancer Cells.
Prajakta R. Tambe (2017)
Reprogramming the T cell Response to Cancer by Simultaneous, Nanoparticle-mediated PD-L1 Inhibition and Immunogenic Cell Death.
Cao Dai Phung (2019)
Synthesis and self-assembly of octenyl succinic anhydride modified short glucan chains based amphiphilic biopolymer: Micelles, ultrasmall micelles, vesicles, and lutein encapsulation/release
Ranran Chang (2017)
Engineered polymeric nanoparticles to guide the cellular internalization and trafficking of small interfering ribonucleic acids
A. E. Arnold (2017)
Evaluation of Phytotoxicity and Mutagenicity of Novel DMAEMA-Containing Gene Carriers
N. Finiuk (2020)
Advances in nanomicelles for sustained drug delivery
Najmeh Amirmahani (2017)
Nanoflower-Shaped Biocatalyst with Peroxidase Activity Enhances the Reversible Addition–Fragmentation Chain Transfer Polymerization of Methacrylate Monomers
Xinghuo Wang (2018)
Codelivery for Paclitaxel and Bcl-2 Conversion Gene by PHB-PDMAEMA Amphiphilic Cationic Copolymer for Effective Drug Resistant Cancer Therapy.
X. Wang (2017)
Poly(l-γ-glutamylglutamine) Polymer Enhances Doxorubicin Accumulation in Multidrug Resistant Breast Cancer Cells
T. Peng (2016)
Production of dry‐state ketoprofen‐encapsulated PMMA NPs by coupling micromixer‐assisted nanoprecipitation and spray drying
Shukai Ding (2019)
Polishing monoclonal antibody using pH-responsive TiO2/polysulfone membrane in dual size-exclusion strategy
H. K. Ng (2019)
Two-stage pH-sensitive doxorubicin hydrochloride loaded core–shell nanoparticles with dual drug-loading strategies for the potential anti-tumor treatment
Xiaoyue Yu (2016)
Multifunctional micelle delivery system for overcoming multidrug resistance of doxorubicin
L. Qin (2018)
Fabrication of aminated poly(glycidyl methacrylate)-based polymers for co-delivery of anticancer drugs and the p53 gene.
L. Li (2020)
Polyester-based nanoparticles for nucleic acid delivery.
J. Zhao (2018)
Functionalized gold nanostructures: promising gene delivery vehicles in cancer treatment
S. Kumar (2019)
Polymeric Nanocarriers of Drug Delivery Systems in Cancer Therapy
N. Avramović (2020)
Combinatorial delivery of superparamagnetic iron oxide nanoparticles (γFe2O3) and doxorubicin using folate conjugated redox sensitive multiblock polymeric nanocarriers for enhancing the chemotherapeutic efficacy in cancer cells.
Chetan Nehate (2017)
A pH-sensitive drug delivery system based on folic acid-targeted HBP-modified mesoporous silica nanoparticles for cancer therapy
Y. Li (2020)
An Integrated Therapeutic Delivery System for Enhanced Treatment of Hepatocellular Carcinoma
Z. Ye (2018)
Dual Drug Backboned Shattering Polymeric Theranostic Nanomedicine for Synergistic Eradication of Patient-Derived Lung Cancer.
Yuwei Cong (2018)
Intelligent polymeric micelles for multidrug co-delivery and cancer therapy
G. Yu (2019)
Preparation, characterization, biotoxicity, and biodistribution of thermo-responsive magnetic complex micelles formed by Mn0.6Zn0.4Fe2O4 and a PCL/PEG analogue copolymer for controlled drug delivery.
X. Wang (2017)See more