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Flexible Gold Electrode Array For Multiplexed Immunoelectrochemical Measurement Of Three Protein Biomarkers For Prostate Cancer.
J. Liu, Cai-Yu Lu, H. Zhou, J. Xu, H. Chen
Published 2014 · Materials Science, Medicine
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In this work, we report a simple and novel electrochemical multiplexed immunosensor on a flexible polydimethylsiloxane (PDMS) slice deposited with 8 × 8 nano-Au film electrodes for simultaneous detection of prostate specific antigen (PSA), prostate specific membrane antigen (PSMA), and interleukin-6 (IL-6). Primary antibodies linked with magnetic beads (Ab1-MBs) were modified on the nano-Au film electrodes via magnetic force. In the presence of corresponding antigen, horse radish peroxidase-secondary antibody-conjugated gold nanorods (HRP-Ab2-gold NRs) were brought into the surface of electrodes, generating obvious electrochemical signals of H2O2 reduction reactions. Based on this, the designed immunosensor provide good performance in sensitivity and specificity during the detection of above three biomarkers for prostate cancer. The electrochemical multiplexed immunosensor was verified for selective and accurate detection of complex samples in human serum. Data suggested that the reported multiplexed immunosensing strategy holds great promise for applications in clinical assay and diseases diagnosis.
This paper references
Protein detection using arrayed microsensor chips: tuning sensor footprint to achieve ultrasensitive readout of CA-125 in serum and whole blood.
Jagotamoy Das (2011)
Improved size-tunable synthesis of monodisperse gold nanorods through the use of aromatic additives.
Xingchen Ye (2012)
Label-free detection of telomerase activity in HeLa cells using electrochemical impedance spectroscopy.
Weiqiang Yang (2011)
Single-wall carbon nanotube forest arrays for immunoelectrochemical measurement of four protein biomarkers for prostate cancer.
Bhaskara V. Chikkaveeraiah (2009)
Detection of prostate cancer with a blood-based assay for early prostate cancer antigen.
B. Paul (2005)
On-chip selective capture of cancer cells and ultrasensitive fluorescence detection of survivin mRNA in a single living cell.
X. Li (2013)
Microchip device with 64-site electrode array for multiplexed immunoassay of cell surface antigens based on electrochemiluminescence resonance energy transfer.
Meisheng Wu (2012)
Cancer nanotechnology: opportunities and challenges
M. Ferrari (2005)
Functional Nanoprobes for Ultrasensitive Detection of Biomolecules
Shiping Song (2011)
Nanogold-enhanced graphene nanosheets as multienzyme assembly for sensitive detection of low-abundance proteins.
H. Chen (2013)
An Electrochemical DNA Biosensor Based on Gold Nanofilm and Stable Y Junction Structure
Xiao-Ya Dong (2012)
Nanoporous gold film based immunosensor for label-free detection of cancer biomarker.
Q. Wei (2011)
Sequential injection chemiluminescence immunoassay for nonionic surfactants by using magnetic microbeads.
R. Zhang (2007)
Prostate-specific antigen and prostate cancer: prediction, detection and monitoring
H. Lilja (2008)
Direct genetic analysis of ten cancer cells: tuning sensor structure and molecular probe design for efficient mRNA capture.
E. Vasilyeva (2011)
Gene therapy for prostate cancer by targeting poly(ADP-ribose) polymerase.
I. Trofimova (2002)
Tumor target prostate specific membrane antigen (PSMA) and its regulation in prostate cancer
A. Ghosh (2004)
Mass spectrometry-based proteomics
R. Aebersold (2003)
Continuous, real-time monitoring of cocaine in undiluted blood serum via a microfluidic, electrochemical aptamer-based sensor.
J. Swensen (2009)
Double-codified gold nanolabels for enhanced immunoanalysis.
A. Ambrosi (2007)
Amplified electrochemical detection of a cancer biomarker by enhanced precipitation using horseradish peroxidase attached on carbon nanotubes.
R. Akter (2012)
Thermal/plasma-driven reversible wettability switching of a bare gold film on a poly(dimethylsiloxane) surface by electroless plating.
J. Wu (2010)
Microfluidic electrochemical immunoarray for ultrasensitive detection of two cancer biomarker proteins in serum.
Bhaskara V. Chikkaveeraiah (2011)
Solution-based circuits enable rapid and multiplexed pathogen detection.
B. Lam (2013)
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Soft and flexible material-based affinity sensors.
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