Online citations, reference lists, and bibliographies.
← Back to Search

Lipoprotein Lipase Expression In Undifferentiated Hepatoma Cells Is Regulated By Progesterone And Protein Kinase A.

J. Peinado-Onsurbe, B. Staels, S. Deeb, J. Auwerx
Published 1992 · Chemistry, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Recently, it was shown that lipoprotein lipase (LPL) was produced in neonatal but not in adult rat liver. In an attempt to further define the mechanism involved in liver LPL expression, we identified a neonatal mouse hepatoma cell line, BWTG3, capable of producing LPL. The regulation of LPL expression by various extracellular stimuli was investigated in this cell line. Progesterone caused a rise in LPL production by BWTG3 cells. Other hormones tested, such as insulin, glucagon, adrenalin, testosterone, and thyroid hormone, had no effect on LPL production. The effects of progesterone on LPL production showed slow kinetics reaching a maximum 24 h after addition. Cotransfection of a progesterone receptor expression vector with a 5'-LPL-CAT reporter construct resulted in an induction of CAT activity, suggesting that the increase in LPL accumulation after progesterone was linked to transcriptional induction of the LPL gene. Stimuli causing an elevation of protein kinase A activity in the cells also increased LPL production. Three agents capable of elevating intracellular cAMP levels, i.e., forskolin, dBcAMP, and choleratoxin, caused an elevation of LPL production. The increase in LPL activity caused by forskolin and choleratoxin was paralleled by an elevation of LPL mRNA levels, while dBcAMP only induced a small elevation of LPL mRNA levels. The increase in LPL production was shown to be linked to the stimulation of the PKA signal transduction pathway and was apparently transmitted via the transcription factor CREB. No effect of the stimulation of protein kinase C or calcium/calmodulin-dependent kinase on LPL production was detected.(ABSTRACT TRUNCATED AT 250 WORDS)
This paper references
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
C. Gorman (1982)
Forskolin: a specific stimulator of adenylyl cyclase or a diterpene with multiple sites of action?
A. Laurenza (1989)
The tissue-specific extinguisher locus TSE1 encodes a regulatory subunit of cAMP-Dependent protein kinase
M. Boshart (1991)
Distribution of lipoprotein lipase and hepatic lipase between plasma and tissues: effect of hypertriglyceridemia.
J. Peterson (1985)
Lipoprotein lipase activity in neonatal-rat liver cell types.
F. Burgaya (1989)
CAT constructs with convenient sites for cloning and generating deletions.
P. Tsonis (1988)
Modulation of lipoprotein lipase activity in cultured rat mesenchymal heart cells and preadipocytes by dibutyryl cyclic AMP, cholera toxin and 3-isobutyl-1-methylxanthine.
G. Friedman (1983)
Characterization of triacylglycerol hydrolase activities in isolated myocardial cells from rat heart.
I. Ramírez (1985)
Carcinogenesis: A superfamily of potentially oncogenic hormone receptors
S. Green (1986)
The relation between glycosylation and activity of guinea pig lipoprotein lipase.
H. Semb (1989)
Pre- and post-natal development of lipoprotein lipase and hepatic triglyceride hydrolase activity in rat tissues.
T. Chajek (1977)
Synthesis of lipoprotein lipase in the liver of newborn rats and localization of the enzyme by immunofluorescence.
S. Vilaró (1988)
Human lipoprotein lipase complementary DNA sequence.
K. Wion (1987)
Secretion and degradation of lipoprotein lipase in cultured adipocytes. Binding of lipoprotein lipase to membrane heparan sulfate proteoglycans is necessary for degradation.
L. Cisar (1989)
Insulin regulation of lipoprotein lipase activity in 3T3-L1 adipocytes is mediated at posttranscriptional and posttranslational levels.
C. Semenkovich (1989)
Transcriptional activation of the lipoprotein lipase and apolipoprotein E genes accompanies differentiation in some human macrophage-like cell lines.
J. Auwerx (1988)
Expression of lipoprotein lipase mRNA in rat heart is localized mainly to mesenchymal cells as studied by in situ hybridization.
O. Stein (1991)
Dibutyryl cAMP-induced increases in triacylglycerol lipase activity in developing L8 myotube cultures.
W. Palmer (1990)
A mouse hepatoma cell line which secretes several serum proteins including albumin and alpha-foetoprotein.
C. Szpirer (1975)
Effectors of lipoprotein lipase secretion from isolated cardiac muscle cells incubated in vitro.
A. Cryer (1981)
Regulation of progesterone receptor-mediated transcription by phosphorylation.
L. Denner (1990)
Lipoprotein lipase gene expression in THP-1 cells.
J. Auwerx (1989)
Effect of combined lipase deficiency (cld/cld) on hepatic and lipoprotein lipase activities in liver and plasma of newborn mice.
T. Olivecrona (1986)
Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREB
K. Yamamoto (1988)
A cyclic AMP response element mediates repression of tyrosine aminotransferase gene transcription by the tissue-specific extinguisher locus Tse-1
M. Boshart (1990)
The S49 Kin- cell line transcribes and translates a functional mRNA coding for the catalytic subunit of cAMP-dependent protein kinase.
S. Orellana (1990)
Structure of the human lipoprotein lipase gene.
S. Deeb (1989)
Effect of Glucocorticoids on Lipoprotein Lipase Activity in Rat Heart and Adipose Tissue
P. D. Gasquet (1975)
Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA.
J. P. Hoeffler (1988)
Starvation enhances lipoprotein lipase activity in the liver of the newborn rat.
D. Grinberg (1985)
The steroid and thyroid hormone receptor superfamily.
R. Evans (1988)
Two genetically defined trans-acting loci coordinately regulate overlapping sets of liver-specific genes
S. Ruppert (1990)
Biosynthesis of lipoprotein lipase in cultured mouse adipocytes. II. Processing, subunit assembly, and intracellular transport.
C. Vannier (1989)
Biosynthesis of lipoprotein lipase in cultured mouse adipocytes. I. Characterization of a specific antibody and relationships between the intracellular and secreted pools of the enzyme.
C. Vannier (1989)
The role of glucose and glycosylation in the regulation of lipoprotein lipase synthesis and secretion in rat adipocytes.
J. Ong (1989)
Subtractive hybridization cloning of a tissue-specific extinguisher: TSE1 encodes a regulatory subunit of protein kinase A
K. W. Jones (1991)
Insulin regulation of lipoprotein lipase activity and release in 3T3-L1 adipocytes. Separation and dependence of hormonal effects on hexose metabolism and synthesis of RNA and protein.
P. Spooner (1979)
Lipoprotein lipase activity in liver of the rat fetus.
M. Llobera (1979)
Intralipid administration induces a lipoprotein lipase-like activity in the livers of starved adult rats.
S. Vilaró (1986)

This paper is referenced by
Insulinorésistance musculaire induite par les céramides : étude des mécanismes d'action et de l'implication du transporteur CERT
Rana Mahfouz (2015)
Hormonal regulation of lipoprotein lipase activity from 5-day-old rat hepatocytes
J. Julve (1996)
Lipoprotein lipase gene expression: physiological regulators at the transcriptional and post-transcriptional level.
S. Enerbäck (1993)
Expression and Regulation of the Lipoprotein Lipase Gene in Human Adrenal Cortex*
B. Staels (1996)
Developmental extinction of liver lipoprotein lipase mRNA expression might be regulated by an NF‐1‐like site
K. Schoonjans (1993)
PAGE4 is a cytoplasmic protein that is expressed in normal prostate and in prostate cancers.
C. Iavarone (2002)
Lipoprotein lipase: recent contributions from molecular biology.
J. Auwerx (1992)
Family study of lipoprotein lipase gene polymorphisms and plasma triglyceride levels
J. Georges (1996)
The lipoprotein lipase gene HindIII polymorphism is associated with lipid levels in early-onset type 2 diabetic patients.
Y. Q. Ma (2003)
Identification of estrogen‐regulated genes in the mouse uterus using a delayed‐implantation model
S. Lee (2003)
Sex steroid receptor expression in different adipose depots is modified during midpregnancy
S. Rodríguez-Cuenca (2006)
MC2, a new phosphodiesterase-3 inhibitor with antilipolytic and hypolipidemic effects in normal and diabetic rats
Bagher Alinejad (2015)
Cilostazol, a selective type III phosphodiesterase inhibitor, decreases triglyceride and increases HDL cholesterol levels by increasing lipoprotein lipase activity in rats.
Takashi Tani (2000)
Protein kinase C and cAMP-dependent protein kinase regulate the neuronal differentiation of immortalized raphe neurons.
H. Rind (1999)
Lipoprotein lipase: structure, function, regulation, and role in disease
J. R. Mead (2002)
GIP increases human adipocyte LPL expression through CREB and TORC2-mediated trans-activation of the LPL gene
S. Kim (2010)
Semantic Scholar Logo Some data provided by SemanticScholar