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Patterning And Biofunctionalization Of Antifouling Hyperbranched Polyglycerol Coatings.
Eli D. Moore, B. Delalat, R. Vasani, H. Thissen, N. Voelcker
Published 2014 · Chemistry, Medicine
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We demonstrate the patterned biofunctionalization of antifouling hyperbranched polyglycerol (HPG) coatings on silicon and glass substrates. The ultralow fouling HPG coatings afforded straightforward chemical handles for rapid bioconjugation of amine containing biomolecular species. This was achieved by sodium periodate oxidation of terminal HPG diols to yield reactive aldehyde groups. Patterned microprinting of sodium periodate and cell adhesion mediating cyclic peptides containing the RGD sequence resulted in an array of covalently immobilized bioactive signals. When incubated with mouse fibroblasts, the HPG background resisted cell attachment whereas high density cell attachment was observed on the peptide spots, resulting in high-contrast cell microarrays. We also demonstrated single-step, in situ functionalization of the HPG coatings by printing periodate and peptide concurrently. Our results demonstrate the effectiveness of antifouling and functionalized HPG graft polymer coatings and establish their use in microarray applications for the first time.
This paper references
Interfaces to control cell-biomaterial adhesive interactions
A. J. García (2006)
Study of single protein adsorption onto monoamino oligoglycerol derivatives: a structure-activity relationship.
M. Wyszogrodzka (2009)
Evaluation of 111In-Labeled Cyclic RGD Peptides: Effects of Peptide and Linker Multiplicity on Their Tumor Uptake, Excretion Kinetics and Metabolic Stability
Jiyun Shi (2011)
Multifunctional protein microarrays for cultivation of cells and immunodetection of secreted cellular products.
C. Jones (2008)
Spot identification and quality control in cell-based microarrays.
M. Bauer (2012)
Micropatterning of cell-repellent polymer on a glass substrate for the highly resolved virus microarray.
K. Na (2006)
New perspectives in cell adhesion: RGD and integrins.
E. Ruoslahti (1987)
Self-assembled monolayers of dendritic polyglycerol derivatives on gold that resist the adsorption of proteins.
Conrad Siegers (2004)
Patterning of peptide nucleic acids using reactive microcontact printing.
Alessandro Calabretta (2011)
Printing proteins as microarrays for high-throughput function determination.
G. MacBeath (2000)
Microarrays of synthetic heparin oligosaccharides.
J. L. de Paz (2006)
Surface engineering for long-term culturing of mesenchymal stem cell microarrays.
Soraya Rasi Ghaemi (2013)
Peptide and small molecule microarray for high throughput cell adhesion and functional assays.
J. Falsey (2001)
Aptamer microarray mediated capture and mass spectrometry identification of biomarker in serum samples.
J. Ahn (2010)
XPS and AFM analysis of antifouling PEG interfaces for microfabricated silicon biosensors.
S. Sharma (2004)
Advanced substrate fabrication for cell microarrays.
A. Hook (2009)
A novel process for inking the stamp with biomacromolecule solution used in reactive microcontact printing.
J. Feng (2004)
Hyperbranched Polyglycidol on Si/SiO2 Surfaces via Surface-Initiated Polymerization
Majad Khan (2003)
Protein microarray technology.
D. Stoll (2002)
On-chip, cell-based microarray immunofluorescence assay for high-throughput analysis of target proteins.
T. Fernandes (2008)
Single-cell microarray for analyzing cellular response.
S. Yamamura (2005)
Controlled Synthesis of Hyperbranched Polyglycerols by Ring-Opening Multibranching Polymerization
A. Sunder (1999)
Polyglycerol coatings of glass vials for protein resistance.
Kerstin Hoeger (2013)
Multifunctional polymer coatings for cell microarray applications.
Mahaveer D. Kurkuri (2009)
Tyramide signal amplification for antibody-overlay lectin microarray: a strategy to improve the sensitivity of targeted glycan profiling.
D. Meany (2011)
A microcontact printing approach to the immobilization of oligonucleotide brushes.
Julia A. Razumovitch (2009)
Novel cellular microarray assay for profiling T-cell peptide antigen specificities.
C. Yue (2010)
Spreading Diagrams for the Optimization of Quill Pin Printed Microarray Density
J. Smith (2002)
Linear and Hyperbranched Polyglycerol Derivatives as Excellent Bioinert Glass Coating Materials
Marie Weinhart (2011)
Hyperbranched polyglycerols at the biointerface
Eli D. Moore (2013)
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Surface-initiated hyperbranched polyglycerol as an ultralow-fouling coating on glass, silicon, and porous silicon substrates.
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Haigang Wu (2020)
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S. Song (2016)
Cationic Copolymerization of 3,3-Bis(hydroxymethyl)oxetane and Glycidol: Biocompatible Hyperbranched Polyether Polyols with High Content of Primary Hydroxyl Groups.
E. Christ (2015)
Amine functionalized polyglycerols obtained by copolymerization of cyclic carbonate monomers
P. Parzuchowski (2018)
Multiple and terminal grafting of linear polyglycidol for surfaces of reduced protein adsorption
Alicja Utrata-Wesołek (2016)
Synthesis of Multifunctional Polyethers: Anionic Ring-Opening Polymerization of Functional Epoxide Monomers
Joonhee Lee (2020)
Hard and soft nanocomposites enabled by rationally designed nonlinear copolymers derived from high functionality polyols
James Iocozzia (2018)
Hyperbranched polyglycerols containing amine groups — Synthesis, characterization and carbon dioxide capture
P. Parzuchowski (2018)
Chapter 4 Polyglycerols in Nanomedicine
Mohiuddin Abdul Quadir (2016)
Enhanced separation and antifouling properties of PVDF ultrafiltration membranes with surface covalent self-assembly of polyethylene glycol
Xinzhen Zhao (2015)
Electron beam lithography of poly(glycidol) nanogels for immobilization of a three-enzyme cascade
Jacob N Lockhart (2018)
Synthesis and Conjugation of Alkyne‐Functional Hyperbranched Polyglycerols
Eli D. Moore (2016)