Online citations, reference lists, and bibliographies.
← Back to Search

Surface Charge Of Nanoparticles Determines Their Endocytic And Transcytotic Pathway In Polarized MDCK Cells.

Oshrat Harush-Frenkel, Eva Rozentur, S. Benita, Y. Altschuler
Published 2008 · Medicine, Chemistry

Cite This
Download PDF
Analyze on Scholarcy
Share
A major challenge in drug delivery is the internalization through the apical plasma membrane of the polarized epithelial cells lining organs facing the external environment, e.g., lungs and the gastrointestinal tract. The reduced permeation of drugs entering through this pathway is in part due to the mucosal barrier and low rate of endocytosis at these membranes. We investigated the possible role of nanoparticle surface charge on its entry through the apical plasma membrane and its intracellular pathway. We found that both cationic and anionic nanoparticles are targeted mainly to the clathrin endocytic machinery. A fraction of both nanoparticle formulations is suspected to internalize through a macropinocytosis-dependent pathway. A significant amount of nanoparticles transcytose and accumulate at the basolateral membrane. Some anionic but not cationic nanoparticles transited through the degradative lysosomal pathway. Taken together, these observations indicate that cationic nanoparticles, in addition to their potential for drug delivery to epithelia, may be promising carriers for transcytosing drugs to the blood stream.



This paper is referenced by
10.1039/c2cs15344k
Targeted polymeric therapeutic nanoparticles: design, development and clinical translation.
Nazila Kamaly (2012)
10.1002/9780470571224.PSE495
Nanocrystals Production, Characterization, and Application for Cancer Therapy
Christin P. Hollis (2013)
10.3109/03639045.2016.1160103
Targeted hepatocellular carcinoma therapy: transferrin modified, self-assembled polymeric nanomedicine for co-delivery of cisplatin and doxorubicin
X. Zhang (2016)
10.1007/s13273-012-0039-z
The biological toxicities of two crystalline phases and differential sizes of TiO2 nanoparticles during zebrafish embryogenesis development
M. Yeo (2012)
10.1038/s41598-018-24860-8
Publisher Correction: Pathways of cellular internalisation of liposomes delivered siRNA and effects on siRNA engagement with target mRNA and silencing in cancer cells
A. Alshehri (2018)
10.1016/j.ejpb.2016.02.014
Antigen delivery via hydrophilic PEG-b-PAGE-b-PLGA nanoparticles boosts vaccination induced T cell immunity.
R. Rietscher (2016)
10.1016/j.jconrel.2012.05.020
On the cellular processing of non-viral nanomedicines for nucleic acid delivery: mechanisms and methods.
D. Vercauteren (2012)
10.1021/nn3059295
Polymer-coated nanoparticles interacting with proteins and cells: focusing on the sign of the net charge.
Dominik Hühn (2013)
10.1021/tx100307h
Internalization of carbon black and maghemite iron oxide nanoparticle mixtures leads to oxidant production.
J. Berg (2010)
10.3390/nano6110192
Cellular Interactions and Formation of an Epithelial “Nanocoating-Like Barrier” with Mesoporous Silica Nanoparticles
X. Li (2016)
10.1002/9780470875780.CH11
Can QSAR Models Describing Small‐Molecule Xenobiotics Give Useful Tips for Predicting Uptake and Localization of Nanoparticles in Living Cells? And If Not, Why Not?
R. Horobin (2010)
10.1080/13813455.2020.1742165
Synthesis, characterisation and enhanced apoptotic effect of gemcitabine-loaded albumin nanoparticles coating with chitosan.
E. Salim (2020)
NANOCRYSTALS OF CHEMOTHERAPEUTIC AGENTS FOR CANCER THERANOSTICS: DEVELOPMENT AND IN VITRO AND IN VIVO EVALUATION
Christin P. Hollis (2012)
10.2147/IJN.S80297
Delivery of baicalein and paclitaxel using self-assembled nanoparticles: synergistic antitumor effect in vitro and in vivo
W. Wang (2015)
10.1016/j.dmpk.2018.10.003
Improvement of pharmacokinetic properties of therapeutic antibodies by antibody engineering.
K. Haraya (2019)
10.1016/B978-0-12-391857-4.00021-5
Nanoparticle PEBBLE sensors in live cells.
Y. Lee (2012)
10.1007/s11095-011-0622-3
Polymeric Nanoparticles Affect the Intracellular Delivery, Antiretroviral Activity and Cytotoxicity of the Microbicide Drug Candidate Dapivirine
J. das Neves (2011)
Étude par microscopie électronique des mécanismes de transport des nanoparticules de silice au travers d'une barrière endothéliale
G. Naudin (2014)
10.1016/J.IMPACT.2017.08.002
In vitro approaches to assess the hazard of nanomaterials
B. Drašler (2017)
10.1002/9780470571224.PSE498
Intracellular Trafficking of Nanoparticles: Implications for Therapeutic Efficacy of the Encapsulated Drug
L. Niu (2013)
10.1007/978-94-017-8896-0_6
Uptake and Intracellular Trafficking of Nanocarriers
H. Andersen (2014)
10.1155/2012/686108
Cationic Albumin Nanoparticles for Enhanced Drug Delivery to Treat Breast Cancer: Preparation and In Vitro Assessment
S. Abbasi (2012)
10.1080/03639045.2018.1539496
Formulation-optimization of solid lipid nanocarrier system of STAT3 inhibitor to improve its activity in triple negative breast cancer cells
S. K. Pindiprolu (2019)
Photonic and Magnetic Nano- and Micro-Particles for Biomedical Applications: Detection and Destruction of Bacterial and Cancer Cells
R. Smith (2013)
10.1021/nn900681c
Nanoshells for surface-enhanced Raman spectroscopy in eukaryotic cells: cellular response and sensor development.
M. A. Ochsenkühn (2009)
10.1007/s11051-013-1985-7
Surfactant-assisted dispersion of carbon nanotubes: mechanism of stabilization and biocompatibility of the surfactant
R. Singh (2013)
10.1002/smll.201100001
Cellular uptake, intracellular trafficking, and cytotoxicity of nanomaterials.
F. Zhao (2011)
10.1016/j.ijpharm.2020.119715
Cell targeting strategy affects the intracellular trafficking of liposomes altering loaded doxorubicin release kinetics and efficacy in endothelial cells.
A. Arta (2020)
10.1039/c5nr00327j
Intracellular sorting of differently charged chitosan derivatives and chitosan-based nanoparticles.
A. Zubareva (2015)
10.1016/B978-0-323-46144-3.00012-X
Design of nanoparticle structures for cancer immunotherapy
Peter Tsirikis (2017)
10.1088/1361-6528/aa6d15
The impact of microfluidic mixing of triblock micelleplexes on in vitro / in vivo gene silencing and intracellular trafficking.
Daniel P Feldmann (2017)
10.3109/09687688.2010.522117
Dynamic and cellular interactions of nanoparticles in vascular-targeted drug delivery (review)
R. B. Huang (2010)
See more
Semantic Scholar Logo Some data provided by SemanticScholar