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Magnetic Field-Activated Sensing Of MRNA In Living Cells

Saira F. Bakshi, N. Guz, A. Zakharchenko, Han Deng, A. Tumanov, C. Woodworth, S. Minko, D. Kolpashchikov, E. Katz
Published 2017 · Chemistry, Medicine

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Detection of specific mRNA in living cells has attracted significant attention in the past decade. Probes that can be easily delivered into cells and activated at the desired time can contribute to understanding translation, trafficking and degradation of mRNA. Here we report a new strategy termed magnetic field-activated binary deoxyribozyme (MaBiDZ) sensor that enables both efficient delivery and temporal control of mRNA sensing by magnetic field. MaBiDZ uses two species of magnetic beads conjugated with different components of a multicomponent deoxyribozyme (DZ) sensor. The DZ sensor is activated only in the presence of a specific target mRNA and when a magnetic field is applied. Here we demonstrate that MaBiDZ sensor can be internalized in live MCF-7 breast cancer cells and activated by a magnetic field to fluorescently report the presence of specific mRNA, which are cancer biomarkers.
This paper references
10.1039/c2cc16654b
Light-activatable molecular beacons with a caged loop sequence.
K. B. Joshi (2012)
10.1098/rsif.2007.1274
Selective activation of mechanosensitive ion channels using magnetic particles
S. Hughes (2007)
10.1016/j.biomaterials.2016.05.022
Single-molecule detection and tracking of RNA transcripts in living cells using phosphorothioate-optimized 2'-O-methyl RNA molecular beacons.
D. Zhao (2016)
10.1002/smll.201401137
Single-cell detection of mRNA expression using nanofountain-probe electroporated molecular beacons.
Juan P. Giraldo-Vela (2015)
10.1002/anie.201303919
Folding of 16S rRNA in a signal-producing structure for the detection of bacteria.
Y. V. Gerasimova (2013)
10.1002/cbic.201000006
RNA‐Cleaving Deoxyribozyme Sensor for Nucleic Acid Analysis: The Limit of Detection
Y. V. Gerasimova (2010)
Gynecologic Onc
Y. Li (2011)
10.1021/acs.analchem.6b02710
Continuous Monitoring of Specific mRNA Expression Responses with a Fluorescence Resonance Energy Transfer-Based DNA Nano-tweezer Technique That Does Not Require Gene Recombination.
Hajime Shigeto (2016)
10.1039/c3cc42420k
Light-inducible molecular beacons for spatio-temporally highly defined activation.
Jennifer S Rinne (2013)
10.1136/jmedgenet-2015-103334
ceRNA in cancer: possible functions and clinical implications
X. Qi (2015)
10.1002/anie.201204203
From one-photon to two-photon probes: "caged" compounds, actuators, and photoswitches.
G. Bort (2013)
10.1002/cbic.200700384
A Binary Deoxyribozyme for Nucleic Acid Analysis
D. Kolpashchikov (2007)
10.1021/nl901517b
Aptamer nano-flares for molecular detection in living cells.
D. Zheng (2009)
10.1093/NAR/GNH062
Dual FRET molecular beacons for mRNA detection in living cells.
P. Santangelo (2004)
10.1039/c1cc10481k
Caged molecular beacons: controlling nucleic acid hybridization with light.
C. Wang (2011)
10.3389/fchem.2016.00029
RNA Fluorescence with Light-Up Aptamers
J. Ouellet (2016)
10.1126/SCIENCE.7684161
Mechanotransduction across the cell surface and through the cytoskeleton
Ning Wang (1993)
10.1021/nn9003814
Nano-flares for mRNA regulation and detection.
Andrew E. Prigodich (2009)
10.1021/ar3000162
Nucleic acid delivery: the missing pieces of the puzzle?
J. Nguyen (2012)
10.1073/pnas.1510581112
Quantification and real-time tracking of RNA in live cells using Sticky-flares
William E. Briley (2015)
10.1016/S0006-3495(99)77225-5
Measurement of local viscoelasticity and forces in living cells by magnetic tweezers.
A. Bausch (1999)
10.1088/0957-4484/19/33/335601
Synthesis and characterization of ultra-small superparamagnetic iron oxide nanoparticles thinly coated with silica.
A. Bumb (2008)
10.1021/acscentsci.6b00054
Nucleic Acid Templated Chemical Reaction in a Live Vertebrate
L. Holtzer (2016)
Expression of twist and wnt in human breast cancer.
O. Watanabe (2004)
10.1172/JCI84422
Investigating long noncoding RNAs using animal models.
M. Feyder (2016)
10.1007/s12551-016-0195-9
Design and development of genetically encoded fluorescent sensors to monitor intracellular chemical and physical parameters
Arno Germond (2016)
10.1016/S1097-2765(00)80143-4
Localization of ASH1 mRNA particles in living yeast.
E. Bertrand (1998)
10.1038/35023621
Mechanical control of cyclic AMP signalling and gene transcription through integrins
C. Meyer (2000)
10.4161/15476286.2014.972853
RNAa in action: From the exception to the norm
D. Guo (2014)
10.1007/s004240050518
A new method for application of force to cells via ferric oxide beads
M. Glogauer (1997)
10.1016/j.jconrel.2015.09.046
Exploring the role of polymer structure on intracellular nucleic acid delivery via polymeric nanoparticles.
C. J. Bishop (2015)
10.1158/0008-5472.CAN-06-1479
Twist transcriptionally up-regulates AKT2 in breast cancer cells leading to increased migration, invasion, and resistance to paclitaxel.
G. Cheng (2007)
10.1016/j.jinorgbio.2015.03.010
Caged oligonucleotides for studying biological systems.
B. K. Ruble (2015)
10.1016/S0927-7765(99)00156-3
'Stealth' corona-core nanoparticles surface modified by polyethylene glycol (PEG): influences of the corona (PEG chain length and surface density) and of the core composition on phagocytic uptake and plasma protein adsorption.
Gref (2000)
10.1515/BC.2003.082
The Magnetofection Method: Using Magnetic Force to Enhance Gene Delivery
C. Plank (2003)
10.1038/nnano.2008.39
Remote control of cellular behaviour with magnetic nanoparticles.
J. Dobson (2008)
10.1021/JA0776529
Nano-flares: probes for transfection and mRNA detection in living cells.
D. S. Seferos (2007)
10.1126/science.1207339
RNA Mimics of Green Fluorescent Protein
Jeremy S Paige (2011)
10.1039/c5cc10147f
FRET-based nanoprobes for simultaneous monitoring of multiple mRNAs in living cells using single wavelength excitation.
W. Pan (2016)
10.4161/rna.35506
RNA imaging in living cells – methods and applications
Martyna O. Urbanek (2014)
10.1016/S0006-3495(01)76234-0
Rapid stiffening of integrin receptor-actin linkages in endothelial cells stimulated with thrombin: a magnetic bead microrheology study.
A. Bausch (2001)
10.1021/ja9076777
MNAzymes, a Versatile New Class of Nucleic Acid Enzymes That Can Function as Biosensors and Molecular Switches
E. Mokany (2010)
10.1038/71889
Synthetic DNA delivery systems
D. Luo (2000)
10.1021/acs.joc.5b01132
Photoinduced Electron-Transfer-Based Hybridization Probes for Detection of DNA and RNA.
Kan Takahashi (2015)
10.1039/c3ob41735b
Caged nucleotides/nucleosides and their photochemical biology.
X. Tang (2013)
10.1016/j.bbrc.2015.12.101
Long non-coding RNA PVT1 and cancer.
M. Cui (2016)
10.1208/aapsj070109
DNA-based therapeutics and DNA delivery systems: A comprehensive review
S. Patil (2008)
10.1016/j.ygyno.2011.09.003
Correlation of TWIST2 up-regulation and epithelial-mesenchymal transition during tumorigenesis and progression of cervical carcinoma.
Y. Li (2012)
10.1021/bc2003567
Improved templated fluorogenic probes enhance the analysis of closely related pathogenic bacteria by microscopy and flow cytometry.
R. Franzini (2011)
10.1039/c4ob00096j
Amplification by nucleic acid-templated reactions.
Julia Michaelis (2014)



This paper is referenced by
10.1016/j.biomaterials.2019.119535
Cationic copolymer-chaperoned DNAzyme sensor for microRNA detection.
Orakan Hanpanich (2019)
10.1002/cphc.201800851
Magneto-Controlled Biocatalytic Cascades with Logically Processed Input Signals - Substrate Channeling versus Free Diffusion.
Yaroslav Filipov (2018)
10.1002/chem.201905528
Bifunctional RNA-targeting deoxyribozyme nanodevice as a potential theranostic agent.
Aleksandr A Spelkov (2020)
10.1016/j.ymeth.2019.03.008
Visualizing mRNA in live mammalian cells.
Zejun Wang (2019)
10.1021/acs.analchem.8b03067
Composition-Tunable Hollow Au/Ag SERS Nanoprobes Coupled with Target-Catalyzed Hairpin Assembly for Triple-Amplification Detection of miRNA.
Y. Sun (2018)
10.1016/J.ELECTACTA.2017.12.190
Ultrasensitive determination of microribonucleic acids in cancer cells with nanostructured-disposable electrodes using the viral protein p19 for recognition of ribonucleic acid/microribonucleic acid homoduplexes
M. Zouari (2018)
10.3390/magnetochemistry6010003
Magnetic Nanoparticles for Nanomedicine
M. Hepel (2020)
10.1039/d0cc02395g
Photocaged FRET nanoflares for intracellular microRNA imaging.
J. Li (2020)
10.1016/j.addr.2019.08.001
RNA imaging by chemical probes.
Fumiaki Tomoike (2019)
10.1039/c7nr08581h
Nanoreactors based on DNAzyme-functionalized magnetic nanoparticles activated by magnetic field.
Saira F. Bakshi (2018)
10.1007/s12274-020-2826-5
Visualized and cascade-enhanced gene silencing by smart DNAzyme-graphene nanocomplex
Lingjie Ren (2020)
10.1039/c9cc09389c
Hydrazone chemistry assisted DNAzyme for the analysis of double targets.
A. Sheng (2019)
10.1016/J.CCR.2018.05.007
Novel SERS labels: Rational design, functional integration and biomedical applications
Beibei Shan (2018)
10.1021/acssensors.8b00850
MicroRNA Detection through DNAzyme-Mediated Disintegration of Magnetic Nanoparticle Assemblies.
Bo Tian (2018)
10.1002/adma.201901743
Nucleic-Acid Structures as Intracellular Probes for Live Cells.
Devleena Samanta (2019)
10.3390/MAGNETOCHEMISTRY5020024
Towards Nanomaterials for Cancer Theranostics: A System of DNA-Modified Magnetic Nanoparticles for Detection and Suppression of RNA Marker in Cancer Cells
Saira F. Bakshi (2019)
10.1016/j.trac.2020.115811
Photoactivatable Fluorescent Probes for Spatiotemporal-Controlled Biosensing and Imaging
Z. Zou (2020)
10.3390/magnetochemistry5040064
Magnetic Particle Bioconjugates: A Versatile Sensor Approach
S. Krishnan (2019)
10.1002/anie.202008413
A smart theranostic nanocapsule for spatiotemporally programmable photo-genetherapy.
Xue Gong (2020)
10.1038/s41565-018-0232-x
Nucleic acid hybridization on an electrically reconfigurable network of gold-coated magnetic nanoparticles enables microRNA detection in blood
Roya Tavallaie (2018)
10.1039/d0ay01605e
Expanding the analytical applications of nucleic acid hybridization using junction probes.
X. Wang (2020)
10.1002/cbic.201800017
FaptaSyme: A Strategy for Converting a Monomer/Oligomer‐Nonselective Aptameric Sensor into an Oligomer‐Selective One
Baggio A. Evangelista (2018)
10.1039/c8nr02725k
Giant enhancement and facile tuning of photoluminescence in a soft anisotropic magneto-gel.
Vaisakh V Mohanan (2018)
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