Online citations, reference lists, and bibliographies.
← Back to Search

Biorecognition Layer Engineering: Overcoming Screening Limitations Of Nanowire-based FET Devices.

R. Elnathan, Moria Kwiat, A. Pevzner, Yoni Engel, L. Burstein, Artium Khatchtourints, A. Lichtenstein, Raisa Kantaev, F. Patolsky
Published 2012 · Materials Science, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
Detection of biological species is of great importance to numerous areas of medical and life sciences from the diagnosis of diseases to the discovery of new drugs. Essential to the detection mechanism is the transduction of a signal associated with the specific recognition of biomolecules of interest. Nanowire-based electrical devices have been demonstrated as a powerful sensing platform for the highly sensitive detection of a wide-range of biological and chemical species. Yet, detecting biomolecules in complex biosamples of high ionic strength (>100 mM) is severely hampered by ionic screening effects. As a consequence, most of existing nanowire sensors operate under low ionic strength conditions, requiring ex situ biosample manipulation steps, that is, desalting processes. Here, we demonstrate an effective approach for the direct detection of biomolecules in untreated serum, based on the fragmentation of antibody-capturing units. Size-reduced antibody fragments permit the biorecognition event to occur in closer proximity to the nanowire surface, falling within the charge-sensitive Debye screening length. Furthermore, we explored the effect of antibody surface coverage on the resulting detection sensitivity limit under the high ionic strength conditions tested and found that lower antibody surface densities, in contrary to high antibody surface coverage, leads to devices of greater sensitivities. Thus, the direct and sensitive detection of proteins in untreated serum and blood samples was effectively performed down to the sub-pM concentration range without the requirement of biosamples manipulation.
This paper references
10.1002/cphc.201000021
Heteroepitaxial Si/ZnO hierarchical nanostructures for future optoelectronic devices.
M. Devika (2010)
10.1021/NL0484189
Novel electrochemical biosensing platform using self-assembled peptide nanotubes.
Miri Yemini (2005)
10.2217/17435889.1.1.51
Nanowire sensors for medicine and the life sciences.
Fernando Patolsky (2006)
10.1021/JA065923U
Quantitative real-time measurements of DNA hybridization with alkylated nonoxidized silicon nanowires in electrolyte solution.
Yuri L Bunimovich (2006)
10.1016/S0009-8981(99)00077-7
Tissue specificity of cardiac troponin I, cardiac troponin T and creatine kinase-MB.
F. Apple (1999)
10.1021/ja808483t
Shape- and dimension-controlled single-crystalline silicon and SiGe nanotubes: toward nanofluidic FET devices.
Moshit Ben Ishai (2009)
10.1021/ja206639d
Non-covalent monolayer-piercing anchoring of lipophilic nucleic acids: preparation, characterization, and sensing applications.
Moria Kwiat (2012)
10.1021/AC069419J
Nanowire-based biosensors.
Fernando Patolsky (2006)
10.1021/nl201527h
Confinement-guided shaping of semiconductor nanowires and nanoribbons: "writing with nanowires".
A. Pevzner (2012)
10.1073/PNAS.0406368102
Label-free detection of small-molecule-protein interactions by using nanowire nanosensors.
W. U. Wang (2005)
10.1021/nl803657z
Pressure-modulated alloy composition in Si((1-x))Ge(x) nanowires.
U. Givan (2009)
10.1021/JP2037944
Controlled Synthesis of Ferromagnetic Semiconducting Silicon Nanotubes
Nava Shpaisman (2012)
10.1021/NL034853B
Direct ultrasensitive electrical detection of DNA and DNA sequence variations using nanowire nanosensors
J. H. and (2004)
10.1126/SCIENCE.271.5251.933
Semiconductor Clusters, Nanocrystals, and Quantum Dots
A. P. Alivisatos (1996)
10.1021/nn9011384
A calibration method for nanowire biosensors to suppress device-to-device variation.
F. Ishikawa (2009)
10.1038/nnano.2009.353
Label-free biomarker detection from whole blood
E. Stern (2010)
10.1126/SCIENCE.291.5505.851
Functional nanoscale electronic devices assembled using silicon nanowire building blocks.
Yunlong Cui (2001)
10.1093/CLINCHEM/43.3.458
Improved troponin T ELISA specific for cardiac troponin T isoform: assay development and analytical and clinical validation.
M. Mueller-Bardorff (1997)
10.1002/adma.200902815
A route to high-quality crystalline coaxial core/multishell Ge@Si(GeSi)(n) and Si@(GeSi)(n) nanowire heterostructures.
Moshit Ben-Ishai (2010)
10.1021/JP010540Y
Synthetic Control of the Diameter and Length of Single Crystal Semiconductor Nanowires
M. Gudiksen (2001)
10.1039/c0nr00442a
Quantifying signal changes in nano-wire based biosensors.
L. De Vico (2011)
10.1557/MRS2003.144
Nanoscale Science and Technology: Building a Big Future from Small Things
C. Lieber (2003)
10.1021/BI00897A024
THE SUBUNITS OF PURIFIED RABBIT ANTIBODY.
S. Utsumi (1964)
10.1038/nbt1138
Multiplexed electrical detection of cancer markers with nanowire sensor arrays
G. Zheng (2005)
10.1002/ADMA.19960081106
High order and submolecular imaging of end-capped quinquethiophene on Ag(111)†
A. Soukopp (1996)
10.1021/ja109197u
Wall-selective chemical alteration of silicon nanotube molecular carriers.
Moshit Ben-Ishai (2011)
10.1021/ac901157x
Label-free electrical detection of cardiac biomarker with complementary metal-oxide semiconductor-compatible silicon nanowire sensor arrays.
J. H. Chua (2009)
10.1016/S1369-7021(04)00286-X
Nanostructures of zinc oxide
Z. Wang (2004)
10.1063/1.2779930
Screening model for nanowire surface-charge sensors in liquid
M. H. Sørensen (2007)
10.1038/nature01083
Initiation and re-initiation of DNA unwinding by the Escherichia coli Rep helicase
T. Ha (2002)
10.1063/1.1387591
Flexible Methods for Microfluidics
G. Whitesides (2001)
10.1073/PNAS.0406159101
Electrical detection of single viruses.
Fernando Patolsky (2004)
10.1016/0924-4247(96)01275-7
The future of biosensors
P. Bergveld (1996)
10.1021/JP0009305
Doping and Electrical Transport in Silicon Nanowires
Y. Cui (2000)
10.1021/AR9700365
Chemistry and Physics in One Dimension: Synthesis and Properties of Nanowires and Nanotubes
J. Hu (1999)
10.1126/SCIENCE.1062711
Nanowire Nanosensors for Highly Sensitive and Selective Detection of Biological and Chemical Species
Yunlong Cui (2001)
10.1002/ADMA.200390087
One‐Dimensional Nanostructures: Synthesis, Characterization, and Applications
Y. Xia (2003)
10.1021/BI00458A002
A comparison of strategies to stabilize immunoglobulin Fv-fragments.
R. Glockshuber (1990)
10.1016/S1369-7021(06)71651-0
Nanowire-based one-dimensional electronics
C. Thelander (2006)
TrAC
A. J. Killard (1995)
10.1073/PNAS.91.3.893
A simpler sort of antibody.
L. Masat (1994)
10.1021/ac9818430
Peer reviewed: nanomaterials in analytical chemistry.
C. Martin (1998)
10.1021/nl903560u
Knocking down highly-ordered large-scale nanowire arrays.
A. Pevzner (2010)
10.1002/anie.201000847
Supersensitive detection of explosives by silicon nanowire arrays.
Yoni Engel (2010)
10.1021/nl204263k
From crystalline germanium-silicon axial heterostructures to silicon nanowire-nanotubes.
Moshit Ben-Ishai (2012)
10.1021/JP910934H
The Influence of Doping on the Chemical Composition, Morphology and Electrical Properties of Si(1−x)Gex Nanowires
U. Givan (2010)
10.1557/MRS2007.47
Nanowire-Based Nanoelectronic Devices in the Life Sciences
Fernando Patolsky (2007)
10.1016/J.NANTOD.2009.04.005
A reversible surface functionalized nanowire transistor to study protein–protein interactions
S. P. Lin (2009)
10.1002/ANIE.200703587
Colorless tetrapyrrolic chlorophyll catabolites found in ripening fruit are effective antioxidants.
T. Müller (2007)



This paper is referenced by
10.1021/acs.nanolett.5b01578
Manipulating and Monitoring On-Surface Biological Reactions by Light-Triggered Local pH Alterations.
Hagit Peretz-Soroka (2015)
10.1002/smll.201203245
Electrical graphene aptasensor for ultra-sensitive detection of anthrax toxin with amplified signal transduction.
Duck-Jin Kim (2013)
10.1002/9780470027318.A9393
Potentiometric Nanostructured Sensors
F. Andrade (2014)
10.1021/ACS.NANOLETT.6B02584
Antigen-Dissociation from Antibody-Modified Nanotransistor Sensor Arrays as a Direct Biomarker Detection Method in Unprocessed Biosamples.
V. Krivitsky (2016)
10.1016/j.mee.2020.111348
Multi-stack insulator to minimise threshold voltage drift in ZnO FET sensors operating in ionic solutions
J. D. Akrofi (2020)
10.1002/advs.201900522
Hybrid Silicon Nanowire Devices and Their Functional Diversity
L. Baraban (2019)
10.1021/acs.chemrev.7b00037
Nanoparticle-Based Immunochemical Biosensors and Assays: Recent Advances and Challenges.
Z. Farka (2017)
10.1016/j.copbio.2012.11.014
Nanotechnology meets electrophysiology.
Moria Kwiat (2013)
10.1039/c7lc00722a
Enabling electrical biomolecular detection in high ionic concentrations and enhancement of the detection limit thereof by coupling a nanofluidic crystal with reconfigurable ion concentration polarization.
W. Ouyang (2017)
10.1109/ACCESS.2015.2422842
Silicon Nanowire Field-Effect Transistors—A Versatile Class of Potentiometric Nanobiosensors
Luye Mu (2015)
10.1016/j.aca.2016.02.042
High-performance integrated field-effect transistor-based sensors.
R. Adzhri (2016)
10.1016/J.SNB.2016.02.057
Improving charge-sensitive biomolecule sensors with the right choice of electrolyte
L. Friedrich (2016)
10.1021/acs.analchem.6b00702
Low-Cost Nanoribbon Sensors for Protein Analysis in Human Serum Using a Miniature Bead-Based Enzyme-Linked Immunosorbent Assay.
Chunxiao Hu (2016)
Development and investigation of metal oxide nanostructures for sensing applications
M. Jakob (2018)
10.1186/s40824-019-0181-y
Electrochemical biosensors: perspective on functional nanomaterials for on-site analysis
Il-Hoon Cho (2020)
10.1002/wnan.1235
Performance limitations for nanowire/nanoribbon biosensors.
N. Rajan (2013)
10.3390/CHEMOSENSORS4040020
Recent Trends in Field-Effect Transistors-Based Immunosensors
A. C. M. Moraes (2016)
10.1038/srep07865
Electrical Detection of Dengue Biomarker Using Egg Yolk Immunoglobulin as the Biological Recognition Element
A. Figueiredo (2015)
10.1002/PAT.3989
Light‐triggered antifouling coatings for porous silicon optical transducers
Y. Bussi (2017)
10.1002/admt.202000380
Interface Engineering of Si Hybrid Nanostructures for Chemical and Biological Sensing
Jinghua Li (2020)
10.2147/IJN.S134441
A strategy to minimize the sensing voltage drift error in a transistor biosensor with a nanoscale sensing gate
Hyun Woo Son (2017)
10.1007/5346_2017_19
Silicon Nanowire Field-Effect Biosensors
Dipti Rani (2018)
10.1021/ac401085c
Attomolar detection of influenza A virus hemagglutinin human H1 and avian H5 using glycan-blotted field effect transistor biosensor.
S. Hideshima (2013)
10.1063/1.5131365
Microfluidic opportunities in printed electrolyte-gated transistor biosensors.
K. Dorfman (2020)
10.1016/j.cossms.2020.100836
Current understanding and emerging applications of 3D crumpling mediated 2D material-liquid interactions
Peter Snapp (2020)
10.1021/acs.nanolett.5b00133
General strategy for biodetection in high ionic strength solutions using transistor-based nanoelectronic sensors.
N. Gao (2015)
10.1149/2.1471706JES
Whole-Cell Electrochemical Biosensor Integrating Microbes with Si Nanowire-Forest
Nofar Mintz-Hemed (2017)
10.1002/anie.201304857
Unwrapping core-shell nanowires into nanoribbon-based superstructures.
A. Pevzner (2013)
10.1016/J.SNB.2015.07.042
Attomolar detection of cytokines using a chemiluminescence immunoassay based on an antibody-arrayed CMOS image sensor
D. Hong (2015)
10.1038/s41578-020-0229-6
Solid-state nanopore sensors
Liang Xue (2020)
10.1039/c3cs60077g
Electrical biosensors and the label free detection of protein disease biomarkers.
X. Luo (2013)
10.3929/ethz-a-010261371
CMOS Circuits for High-Density neural Interfaces and Nanowire Sensor Arrays
Paolo Livi (2014)
See more
Semantic Scholar Logo Some data provided by SemanticScholar