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A Rapid Pathway Toward A Superb Gene Delivery System: Programming Structural And Functional Diversity Into A Supramolecular Nanoparticle Library.

Hao Wang, Kan Liu, K. Chen, Yujie Lu, S. Wang, Wei-Yu Lin, Feng Guo, K. Kamei, Y. Chen, M. Ohashi, M. Wang, M. A. Garcia, X. Zhao, C. K. Shen, H. Tseng
Published 2010 · Materials Science, Medicine

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Nanoparticles are regarded as promising transfection reagents for effective and safe delivery of nucleic acids into a specific type of cells or tissues providing an alternative manipulation/therapy strategy to viral gene delivery. However, the current process of searching novel delivery materials is limited due to conventional low-throughput and time-consuming multistep synthetic approaches. Additionally, conventional approaches are frequently accompanied with unpredictability and continual optimization refinements, impeding flexible generation of material diversity creating a major obstacle to achieving high transfection performance. Here we have demonstrated a rapid developmental pathway toward highly efficient gene delivery systems by leveraging the powers of a supramolecular synthetic approach and a custom-designed digital microreactor. Using the digital microreactor, broad structural/functional diversity can be programmed into a library of DNA-encapsulated supramolecular nanoparticles (DNA⊂SNPs) by systematically altering the mixing ratios of molecular building blocks and a DNA plasmid. In vitro transfection studies with DNA⊂SNPs library identified the DNA⊂SNPs with the highest gene transfection efficiency, which can be attributed to cooperative effects of structures and surface chemistry of DNA⊂SNPs. We envision such a rapid developmental pathway can be adopted for generating nanoparticle-based vectors for delivery of a variety of loads.
This paper references
10.1126/science.1125559
Oligonucleotide-Modified Gold Nanoparticles for Intracellular Gene Regulation
N. Rosi (2006)
10.1002/ADMA.200703183
Applications of Nanoparticles in Biology
M. De (2008)
10.1002/SMLL.200700351
Nanoplatforms for targeted molecular imaging in living subjects.
W. Cai (2007)
10.1039/b821324k
Synthesis of micro and nanostructures in microfluidic systems.
S. Marre (2010)
10.1146/ANNUREV.BIOENG.9.060906.152025
Nanotechnology applications in cancer.
Shuming Nie (2007)
10.1002/CHIN.200837267
A Combinatorial Polymer Library Approach Yields Insight into Nonviral Gene Delivery
J. Green (2008)
10.1126/science.1118919
Multistep Synthesis of a Radiolabeled Imaging Probe Using Integrated Microfluidics
C. Lee (2005)
10.1038/nbt1402
A combinatorial library of lipid-like materials for delivery of RNAi therapeutics
A. Akinc (2008)
10.1016/j.addr.2009.03.003
Lipid-based systemic delivery of siRNA.
Yu Cheng Tseng (2009)
10.1002/1521-3773(20011119)40:22<4128::AID-ANIE4128>3.0.CO;2-S
Nanoparticles, Proteins, and Nucleic Acids: Biotechnology Meets Materials Science.
C. Niemeyer (2001)
10.1038/nrg1577
Towards safe, non-viral therapeutic gene expression in humans
D. Glover (2005)
10.1038/nnano.2007.108
Mesoporous silica nanoparticles deliver DNA and chemicals into plants.
F. Torney (2007)
10.1002/anie.200900063
A supramolecular approach for preparation of size-controlled nanoparticles.
H. Wang (2009)
10.1126/SCIENCE.1093783
Selective Differentiation of Neural Progenitor Cells by High-Epitope Density Nanofibers
G. Silva (2004)
10.1002/anie.200905126
Selective inhibition of human brain tumor cells through multifunctional quantum-dot-based siRNA delivery.
Jongjin Jung (2010)
10.1016/j.stem.2008.11.008
Guidelines and techniques for the generation of induced pluripotent stem cells.
N. Maherali (2008)
10.1038/nrg2006
Strategies for silencing human disease using RNA interference
D. Kim (2007)
10.1002/bip.20989
Cell penetrating peptide-modified pharmaceutical nanocarriers for intracellular drug and gene delivery.
V. Torchilin (2008)
10.1021/bc700385r
Enhancement of star vector-based gene delivery to endothelial cells by addition of RGD-peptide.
A. Ishikawa (2008)
Nanoparticles, Proteins, And Nucleic Acids
C. M. Niemeyer (2001)
10.1002/CHIN.201142270
Integrated Microfluidic Reactors
Wei-Yu Lin (2011)
10.1073/PNAS.92.8.3156
Quantitative measurement of intraorganelle pH in the endosomal-lysosomal pathway in neurons by using ratiometric imaging with pyranine.
C. Overly (1995)
10.1038/nrd2614
Nanoparticle therapeutics: an emerging treatment modality for cancer
M. Davis (2008)
10.1038/nature05058
The origins and the future of microfluidics
G. Whitesides (2006)
10.1038/nnano.2006.170
In vivo biodistribution and highly efficient tumour targeting of carbon nanotubes in mice.
Z. Liu (2007)
10.1039/b923711a
A small library of DNA-encapsulated supramolecular nanoparticles for targeted gene delivery.
H. Wang (2010)
Integrin alpha-v-beta-3 Targeted Gene Delivery Using RGD Peptidomimetic Conjugates with Copolymers o
Olivia M. Merkel (2009)
10.1038/nmat2444
Intravaginal gene silencing using biodegradable polymer nanoparticles densely loaded with small-interfering RNA
K. Woodrow (2009)
10.1038/nrc1566
Cancer nanotechnology: opportunities and challenges
M. Ferrari (2005)
10.1021/bm900370p
Virus-inspired approach to nonviral gene delivery vehicles.
Raghunath Roy (2009)
10.1016/J.PROGPOLYMSCI.2008.08.003
Bioinspired application of dendrimers: From bio-mimicry to biomedical applications
Woo Dong Jang (2009)
10.1038/nnano.2008.30
Nanoparticle-mediated cellular response is size-dependent.
W. Jiang (2008)
10.1021/JA00166A021
Cooperative binding by aggregated mono-6-(alkylamino)-.beta.-cyclodextrins
R. C. Petter (1990)
10.1126/science.1076996
Microfluidic Large-Scale Integration
T. Thorsen (2002)
10.1039/b917851a
Microfluidic stochastic confinement enhances analysis of rare cells by isolating cells and creating high density environments for control of diffusible signals.
Meghan E Vincent (2010)
Bioresponsive polymers for nonviral gene delivery.
Haijun Yu (2009)
10.1038/nmat2344
Physical approaches to biomaterial design.
S. Mitragotri (2009)
10.1038/nchem.432
Dynamic hook-and-eye nanoparticle sponges.
R. Klajn (2009)
10.1021/mp900105q
Synthetic viruslike particles for targeted gene delivery to alphavbeta3 integrin-presenting endothelial cells.
G. Zuber (2009)



This paper is referenced by
10.1002/smll.201501348
Soft Supramolecular Nanoparticles by Noncovalent and Host-Guest Interactions.
Carmen Stoffelen (2016)
10.1038/srep07471
Recycling Gene Carrier with High Efficiency and Low Toxicity Mediated by L-Cystine-Bridged Bis(β-cyclodextrin)s
Yu-hui Zhang (2014)
10.1088/0957-4484/27/8/085106
Synthetic nanoparticles camouflaged with biomimetic erythrocyte membranes for reduced reticuloendothelial system uptake.
L. Rao (2016)
10.1021/ar500055s
Cyclodextrin-based host-guest supramolecular nanoparticles for delivery: from design to applications.
Q. Hu (2014)
10.1021/acs.chemrev.5b00346
Degradable Controlled-Release Polymers and Polymeric Nanoparticles: Mechanisms of Controlling Drug Release.
Nazila Kamaly (2016)
10.1021/cr5006793
Nucleic Acid Therapeutics Using Polyplexes: A Journey of 50 Years (and Beyond).
Ulrich Lächelt (2015)
10.1007/s13346-020-00724-5
Cyclodextrins in drug delivery: applications in gene and combination therapy
Rebecca M. Haley (2020)
Nanocapsule-based Platforms for Protein Delivery and Protein Therapy
Yang Liu (2016)
10.1016/j.ijpharm.2019.118898
Multi-functional self-assembled nanoparticles for pVEGF-shRNA loading and anti-tumor targeted therapy.
Sai Lu (2019)
10.1039/C9MH00166B
Stimuli-responsive cyclodextrin-based nanoplatforms for cancer treatment and theranostics
Xikuang Yao (2019)
10.1126/scitranslmed.3003651
Preclinical Development and Clinical Translation of a PSMA-Targeted Docetaxel Nanoparticle with a Differentiated Pharmacological Profile
Jeffrey Hrkach (2012)
10.1002/9781119450467.ch11
Chitosan‐Based Drug Delivery Systems
Cristian Peptu (2019)
10.1039/c4bm00448e
Supramolecular hydrogels: synthesis, properties and their biomedical applications.
Ruijiao Dong (2015)
10.1038/nnano.2012.168
Microfluidic technologies for accelerating the clinical translation of nanoparticles.
P. Valencia (2012)
10.1021/acsnano.5b06860
Pretargeted Positron Emission Tomography Imaging That Employs Supramolecular Nanoparticles with in Vivo Bioorthogonal Chemistry.
Shuang Hou (2016)
10.1088/0957-4484/26/35/355703
pH-Sensitive polymer assisted self-aggregation of bis(pyrene) in living cells in situ with turn-on fluorescence.
Zhongyu Duan (2015)
10.1039/C3BM00189J
Microfluidic Assembly of Cationic-β-Cyclodextrin:Hyaluronic Acid-Adamantane Host:Guest pDNA Nanoparticles.
Aditya Kulkarni (2013)
10.1002/mabi.201100097
Size-controlled nanoassemblies based on cyclodextrin-modified dextrans.
V. Wintgens (2011)
10.1002/adma.201301202
Host-guest supramolecular nanosystems for cancer diagnostics and therapeutics.
L. Wang (2013)
10.1007/978-1-4614-7876-8_18
Cancer-Specific Ligand–Receptor Interactions
Ewelina Kluza (2013)
10.1016/B978-0-12-800148-6.00008-0
Polymers for nucleic acid transfer-an overview.
E. Wagner (2014)
10.1002/anie.201507546
A High-Throughput Platform for Formulating and Screening Multifunctional Nanoparticles Capable of Simultaneous Delivery of Genes and Transcription Factors.
Yang Liu (2016)
10.1002/9780470661345.SMC194
Supramolecular Nanoparticles for Molecular Diagnostics and Therapeutics
Kuan-Ju Chen (2012)
10.1007/S10404-013-1227-4
An automatic microfluidic sample transfer and introduction system
Kan Liu (2014)
10.7287/peerj.preprints.27669v1
A microfluidic approach for sequential assembly of siRNA polyplexes with a defined structure-activity relationship
Dominik M. Loy (2019)
10.1039/C1JM13693C
Silver(I)–glutathione biocoordination polymer hydrogel: effective antibacterial activity and improved cytocompatibility
Y. Liu (2011)
10.1021/acsnano.6b06200
Cross-Linked Fluorescent Supramolecular Nanoparticles as Finite Tattoo Pigments with Controllable Intradermal Retention Times.
Jin-Sil Choi (2017)
10.1021/nn5003024
Molecular Recognition Enables Nanosubstrate-Mediated Delivery of Gene-Encapsulated Nanoparticles with High Efficiency
J. Peng (2014)
10.1039/C4MH00103F
Self-assembly of size-tunable supramolecular nanoparticle clusters in a microfluidic channel
Carmen Stoffelen (2014)
10.1002/advs.201903432
Dual Supramolecular Nanoparticle Vectors Enable CRISPR/Cas9‐Mediated Knockin of Retinoschisin 1 Gene—A Potential Nonviral Therapeutic Solution for X‐Linked Juvenile Retinoschisis
S. Chou (2020)
10.1039/c2cs15344k
Targeted polymeric therapeutic nanoparticles: design, development and clinical translation.
Nazila Kamaly (2012)
10.1155/2012/935325
Optofluidics for Lab-on-a-Chip
Eric Pei-Yu Chiou (2012)
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