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Carbon-nanotube-embedded Hydrogel Sheets For Engineering Cardiac Constructs And Bioactuators.
S. Shin, S. M. Jung, Momen Zalabany, Keekyoung Kim, P. Zorlutuna, S. Kim, M. Nikkhah, M. Khabiry, M. Azize, J. Kong, Kai-Tak Wan, T. Palacios, M. Dokmeci, Hojae Bae, X. Tang, A. Khademhosseini
Published 2013 · Materials Science, Medicine
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We engineered functional cardiac patches by seeding neonatal rat cardiomyocytes onto carbon nanotube (CNT)-incorporated photo-cross-linkable gelatin methacrylate (GelMA) hydrogels. The resulting cardiac constructs showed excellent mechanical integrity and advanced electrophysiological functions. Specifically, myocardial tissues cultured on 50 μm thick CNT-GelMA showed 3 times higher spontaneous synchronous beating rates and 85% lower excitation threshold, compared to those cultured on pristine GelMA hydrogels. Our results indicate that the electrically conductive and nanofibrous networks formed by CNTs within a porous gelatin framework are the key characteristics of CNT-GelMA leading to improved cardiac cell adhesion, organization, and cell-cell coupling. Centimeter-scale patches were released from glass substrates to form 3D biohybrid actuators, which showed controllable linear cyclic contraction/extension, pumping, and swimming actuations. In addition, we demonstrate for the first time that cardiac tissues cultured on CNT-GelMA resist damage by a model cardiac inhibitor as well as a cytotoxic compound. Therefore, incorporation of CNTs into gelatin, and potentially other biomaterials, could be useful in creating multifunctional cardiac scaffolds for both therapeutic purposes and in vitro studies. These hybrid materials could also be used for neuron and other muscle cells to create tissue constructs with improved organization, electroactivity, and mechanical integrity.
This paper references
Cell-laden microengineered gelatin methacrylate hydrogels.
J. W. Nichol (2010)
ff ects of Substrate Mechanics on Contractility of Cardiomyocytes Generated from Human Pluripotent Stem Cells
L. B. Hazeltine (2012)
Carbon nanotubes might improve neuronal performance by favouring electrical shortcuts.
Giada Cellot (2009)
Role of superoxide, nitric oxide, and peroxynitrite in doxorubicin-induced cell death in vivo and in vitro.
Partha Mukhopadhyay (2009)
The electrical stimulation of carbon nanotubes to provide a cardiomimetic cue to MSCs.
E. Mooney (2012)
Carbon nanotube substrates boost neuronal electrical signaling.
Viviana Lovat (2005)
Muscular Thin Films for Building Actuators and Powering Devices
A. Feinberg (2007)
Nanoengineering the heart: conductive scaffolds enhance connexin 43 expression.
Jin-Oh You (2011)
Modeling of cardiac muscle thin films: pre-stretch, passive and active behavior.
J. Shim (2012)
Influence of substrate stiffness on the phenotype of heart cells
Bashir Bhana (2010)
Mechanism of heptanol-induced uncoupling of cardiac gap junctions: a perforated patch-clamp study.
B. R. Takens-Kwak (1992)
Microfabricated biomaterials for engineering 3D tissues.
P. Zorlutuna (2012)
Selective Differentiation of Neural Progenitor Cells by High-Epitope Density Nanofibers
G. Silva (2004)
Hydrogels for tissue engineering: scaffold design variables and applications.
Jeanie L Drury (2003)
Model-based control of cardiac alternans in Purkinje fibers.
A. Garzón (2011)
Protective effect of gap junction uncouplers given during hypoxia against reoxygenation injury in isolated rat hearts.
A. Rodríguez-Sinovas (2006)
A tissue-engineered jellyfish with biomimetic propulsion
J. Nawroth (2012)
Mutations Resulting in the Development of ARVC/D Could Affect Cells of the Cardiac Conduction System
S Pieperhoff (2012)
Nanotechnological strategies for engineering complex tissues.
Tal Dvir (2011)
In vivo fluorescence imaging in the second near-infrared window with long circulating carbon nanotubes capable of ultrahigh tumor uptake.
J. Robinson (2012)
Multi-material bio-fabrication of hydrogel cantilevers and actuators with stereolithography.
V. Chan (2012)
Treatment of acute thromboembolism in mice using heparin-conjugated carbon nanocapsules.
Alan C. L. Tang (2012)
Gene Mutations Resulting in the Development of ARVC/D Could Affect Cells of the Cardiac Conduction System
S. Pieperhoff (2012)
Tough supersoft sponge fibers with tunable stiffness from a DNA self-assembly technique.
C. K. Lee (2009)
Directed 3D cell alignment and elongation in microengineered hydrogels.
H. Aubin (2010)
Bio-hybrid muscle cell-based actuators
L. Ricotti (2012)
Cardiogenesis and the Complex Biology of Regenerative Cardiovascular Medicine
K. Chien (2008)
On the Cytotoxicity of CNT
M. A. Hussain (2009)
Accordion-Like Honeycombs for Tissue Engineering of Cardiac Anisotropy
G. Engelmayr (2008)
A Tissue - Engineered Jelly fi sh with Biomimetic Propulsion
A. Khademhosseini (2012)
Microfabrication of complex porous tissue engineering scaffolds using 3D projection stereolithography.
Robert Gauvin (2012)
Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering
M. Lutolf (2005)
On the cytotoxicity of carbon nanotubes
M. A. Hussain (2009)
Cerium oxide nanoparticles protect cardiac progenitor cells from oxidative stress.
F. Pagliari (2012)
Carbon nanotube reinforced hybrid microgels as scaffold materials for cell encapsulation.
S. R. Shin (2012)
Effects of Substrate Mechanics on Contractility of Cardiomyocytes Generated from Human Pluripotent Stem Cells
Laurie B. Hazeltine (2012)
Autonomic markers of emotional processing: skin sympathetic nerve activity in humans during exposure to emotionally charged images
R. Brown (2012)
Functional cardiac tissue engineering.
Brian Liau (2012)
Rapidly recovering hydrogel scaffolds from self-assembling diblock copolypeptide amphiphiles
A. Nowak (2002)
Carbon Nanotube Reinforced Hybrid Microgels as Scaffold Materials for Cell Encapsulation. ACS Nano
Sr Shin (2012)
Nanowired three dimensional cardiac patches
Tal Dvir (2011)
Substrate flexibility regulates growth and apoptosis of normal but not transformed cells.
H. Wang (2000)
Electrical stimulation systems for cardiac tissue engineering
N. Tandon (2009)
A cell-based biosensor for real-time detection of cardiotoxicity using lensfree imaging.
S. Kim (2011)
Carbon nanotubes promote growth and spontaneous electrical activity in cultured cardiac myocytes.
V. Martinelli (2012)
Myocardial tissue engineering: toward a bioartificial pump
H. Sekine (2011)
Microfluidic patterning for fabrication of contractile cardiac organoids
A. Khademhosseini (2007)
Microfluidic Patterning for Fabricationof Contractile CardiacOrganoids
A. Khademhosseini (2007)
Myotube assembly on nanofibrous and micropatterned polymers.
N. Huang (2006)
Degree of chemical functionalization of carbon nanotubes determines tissue distribution and excretion profile.
K. Al-Jamal (2012)
Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer cell destruction.
N. W. Kam (2005)
Carbon NanotubeReinforcedHybridMicrogels as Sca ff oldMaterials for Cell Encapsulation
S. R. Shin (2012)
Carbon Nanotubes as Free-Radical Scavengers
A. Galano (2008)
Protective effects of fullerenol C60(OH)24 against doxorubicin-induced cardiotoxicity and hepatotoxicity in rats with colorectal cancer.
R. Injac (2009)
This paper is referenced by
Living Materials Herald a New Era in Soft Robotics.
Clement Appiah (2019)
In Situ Generated Medical Devices.
D. Cohn (2019)
Gold Nanoparticles for Tissue Engineering
M. Borzenkov (2018)
Can Carbon Nanotubes Deliver on Their Promise in Biology? Harnessing Unique Properties for Unparalleled Applications
C. Serpell (2016)
Engineering the heart: Evaluation of conductive nanomaterials for improving implant integration and cardiac function
J. Zhou (2014)
Biologically Inspired Micro- and Nanoengineering Systems for Functional and Complex Tissues
Nanoparticle-hydrogel superstructures for biomedical applications.
Y. Jiang (2020)
Tough photoluminescent hydrogels doped with lanthanide.
M. Wang (2015)
Electroconductive Gelatin Methacryloyl-PEDOT:PSS Composite Hydrogels: Design, Synthesis, and Properties.
Andrew R Spencer (2018)
Conductive Silk-Polypyrrole Composite Scaffolds with Bioinspired Nanotopographic Cues for Cardiac Tissue Engineering.
J. Tsui (2018)
Bioelectronics goes 3D: new trends in cell-chip interface engineering.
F. A. Pennacchio (2018)
Nanotechnology-Based Stem Cell Tissue Engineering with a Focus on Regeneration of Cardiovascular Systems
Srikanth Sivaraman (2019)
Graphene: A versatile platform for nanotheranostics and tissue engineering
R. Bai (2018)
Engineering Nanobiomaterials for Improved Tissue Regeneration
L. Xie (2017)
Facile One‐Step Micropatterning Using Photodegradable Gelatin Hydrogels for Improved Cardiomyocyte Organization and Alignment
Kelly M. C. Tsang (2015)
Ion-linked double-network hydrogel with high toughness and stiffness
J. Wang (2015)
Multifunctional 3D printing of heterogeneous hydrogel structures
A. Nadernezhad (2016)
Electrohydrodynamic 3D printing of layer-specifically oriented, multiscale conductive scaffolds for cardiac tissue engineering.
Q. Lei (2019)
Shaping surface acoustic waves for cardiac tissue engineering
Shahid Naseer (2016)
Alginate-Collagen Fibril Composite Hydrogel
M. Baniasadi (2015)
Bioprinting 3 D microfibrous scaffolds for engineering endothelialized myocardium and heart-ona-chip
Y. Zhang (2016)
Cerium- and Iron-Oxide-Based Nanozymes in Tissue Engineering and Regenerative Medicine
Michelle M. T. Jansman (2019)
Cardiac cell differentiation of muscle satellite cells on aligned composite electrospun polyurethane with reduced graphene oxide
Masoumeh Azizi (2019)
Hybrid hydrogels containing vertically aligned carbon nanotubes with anisotropic electrical conductivity for muscle myofiber fabrication
S. Ahadian (2014)
Improving cardiac myocytes performance by carbon nanotubes platforms†
V. Martinelli (2013)
A PNIPAAm-based thermosensitive hydrogel containing SWCNTs for stem cell transplantation in myocardial repair.
Xia Li (2014)
Improved Mechanical Properties and Sustained Release Behavior of Cationic Cellulose Nanocrystals Reinforeced Cationic Cellulose Injectable Hydrogels.
Jun You (2016)
Rational Design of a Conductive Collagen Heart Patch.
Peter C. Sherrell (2017)
3D Bioprinting in Tissue Engineering for Medical Applications: The Classic and the Hybrid
Zelong Xie (2020)
Superior Mechanical Properties of Double-Network Hydrogels Reinforced by Carbon Nanotubes without Organic Modification
W. Dong (2013)
Nanocomposite Hydrogels and Their Applications in Tissue Engineering
A. Motealleh (2017)
Nano-fonctionnalisation des hydrogels naturels bioactifs sous forme de matrice 3D
R. Kadri (2015)See more