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Chitosan And Chitosan/Ethylene Oxide-Propylene Oxide Block Copolymer Nanoparticles As Novel Carriers For Proteins And Vaccines

P. Calvo, C. Remuñán-López, J. L. Vila-jato, M. Alonso
Published 2004 · Medicine, Chemistry
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AbstractPurpose. The aim of this study was to investigate the interaction between the components of novel chitosan (CS) and CS/ethylene oxide-propylene oxide block copolymer (PEO-PPO) nanoparticles and to evaluate their potential for the association and controlled release of proteins and vaccines. Methods. The presence of PEO-PPO on the surface of the nanoparticles and its interaction with the CS was identified by X-ray photoelectron spectroscopy (XPS). The mechanism of protein association was elucidated using several proteins, bovine serum albumin (BSA), and tetanus and diphtheria toxoids, and varying the formulation conditions (different pH values and concentrations of PEO-PPO), and the stage of protein incorporation into the nanoparticles formation medium. Results. BSA and tetanus and diphtheria toxoids were highly associated with CS nanoparticles partly due to electrostatic interactions between the carboxyl groups of the protein and the amine groups of CS. PEO-PPO also interacted electrostatically with CS, thus competing with the proteins for association with CS nanoparticles. A visible amount of PEO-PPO was projected towards the outer phase of the nanoparticles. Proteins were released from the nanoparticles at an almost constant rate, the intensity of which was closely related to the protein loading. Furthermore, the tetanus vaccine was released in the active form for at least 15 days. Conclusions. CS and CS/PEO-PPO nanoparticles prepared by a very mild ionic crosslinking technique are novel and suitable systems for the entrapment and controlled release of proteins and vaccines.



This paper is referenced by
DIFFERENT TECHNIQUES FOR PREPARATION OF POLYMERIC NANOPARTICLES-A
Hemant Kumar Yadav (2012)
10.1007/S40995-017-0152-X
Effect of Fabrication Parameters on the Physiochemical Properties of Amphiphilic Chitosan Nanoparticles
Marjan Motiei (2018)
DETERMINATION OF THE OPTIMUM CONDITIONS FOR PRODUCTION OF CHITOSAN NANOPARTICLES
Amir Doustgani (2007)
POLYSACCHARIDE NANOPARTICLES: PREPARATION AND THEIR POTENTIAL APPLICATION AS DRUG DELIVERY SYSTEMS
V. N. L. Sirisha (2015)
10.1007/12_2012_170
Sizing, Shaping and Pharmaceutical Applications of Polyelectrolyte Complex Nanoparticles
Matthias Dr.-Ing. Müller (2012)
10.1016/j.ejps.2012.04.013
Development and evaluation of rivastigmine loaded chitosan nanoparticles for brain targeting.
Mohammad Fazil (2012)
10.1007/978-3-030-44921-6
Nanopharmaceuticals: Principles and Applications Vol. 2
Vinod Kumar Yata (2020)
10.5958/2231-5713.2015.00008.2
A Review- Emerging Use of Nano-Based Carriers in Diagnosis and Treatment of Cancer-Novel Approaches
Yaqub Khan (2015)
10.1002/9781119041559.CH16
Biodegradable and Biocompatible Polymers‐Based Drug Delivery Systems for Cancer Therapy
Ibrahim M. El-Sherbiny (2015)
10.3390/ma10080929
Smart Carriers and Nanohealers: A Nanomedical Insight on Natural Polymers
Sreejith Raveendran (2017)
10.1016/j.vaccine.2010.01.011
Chitosan-based nanoparticles for improving immunization against hepatitis B infection.
Cecilia Prego (2010)
10.1007/s00253-015-6384-9
Current understanding of synergistic interplay of chitosan nanoparticles and anticancer drugs: merits and challenges
Prameela Kandra (2015)
10.1016/j.colsurfb.2014.11.037
Effect of polymer molecular weight on chitosan-protein interaction.
Laurent Adonis Bekale (2015)
10.1016/J.AJPS.2014.09.005
Chlorogenic acid loaded chitosan nanoparticles with sustained release property, retained antioxidant activity and enhanced bioavailability
Ilaiyaraja Nallamuthu (2015)
10.1007/978-3-319-41129-3_2
Nanoparticles Types, Classification, Characterization, Fabrication Methods and Drug Delivery Applications
Saurabh Bhatia (2016)
10.3109/03639045.2015.1093496
Formulation, optimization and characterization of cationic polymeric nanoparticles of mast cell stabilizing agent using the Box–Behnken experimental design
Balaram Gajra (2016)
10.1016/j.ejps.2016.01.004
Chitosan-pectin hybrid nanoparticles prepared by coating and blending techniques.
Antonio Rampino (2016)
10.1016/j.ejps.2017.04.017
Impact of dose and surface features on plasmatic and liver concentrations of biodegradable polymeric nanocapsules
Líliam Teixeira Oliveira (2017)
10.1016/J.JDDST.2017.06.018
Advances on the formulation of proteins using nanotechnologies
Irene Santalices (2017)
10.1208/pt070250
Studies on effect of pH on cross-linking of chitosan with sodium tripolyphosphate: A technical note
Devika R. Bhumkar (2008)
Chapter 2 Design , Development and Evaluation of Nanoparticulate Based Carrier Systems for Ocular Drug delivery
(2015)
10.1080/03639045.2020.1730396
Riluzole-loaded nanoparticles to alleviate the symptoms of neurological disorders by attenuating oxidative stress
Bushra Nabi (2020)
10.4155/tde-2016-0027
Functionalized diterpene parvifloron D-loaded hybrid nanoparticles for targeted delivery in melanoma therapy.
C. Silva (2016)
10.1016/j.nano.2009.06.002
Preparation of chitosan nanoparticles containing Naja naja oxiana snake venom.
Naser Mohammadpourdounighi (2010)
10.1080/17458080903513284
Two modelling data analytical methods applied to optimise the preparation of norcantharidin chitosan nanoparticles
W. Zhang (2010)
Preparation, characterization and in-vitro drug release evaluation of Ganciclovir loaded Chitosan nanoparticles
Rutu Patel (2016)
10.1016/J.CARBPOL.2011.09.074
Rheological properties of chitosan–tripolyphosphate complexes: From suspensions to microgels
Ji Li (2012)
10.3109/10717544.2014.1002947
Biodegradable intranasal nanoparticulate drug delivery system of risedronate sodium for osteoporosis
Mohammad Fazil (2016)
10.1002/jps.24557
Intranasal Piperine-Loaded Chitosan Nanoparticles as Brain-Targeted Therapy in Alzheimer's Disease: Optimization, Biological Efficacy, and Potential Toxicity.
Yosra S.R. Elnaggar (2015)
10.3390/md14050090
Effect of Experimental Parameters on Alginate/Chitosan Microparticles for BCG Encapsulation
Liliana Aranha Caetano (2016)
10.1016/j.ijbiomac.2016.04.070
Development of chitosan nanoparticles coated with hyaluronic acid for topical ocular delivery of dexamethasone.
Mohd Abul Kalam (2016)
10.1016/j.msec.2016.05.121
Polysaccharide based nanogels in the drug delivery system: Application as the carrier of pharmaceutical agents.
Tilahun Ayane Debele (2016)
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