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Gene Expression Profile In Response To Chromium-induced Cell Stress In A549 Cells

J. Ye, X. Shi
Published 2004 · Medicine

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Chromium (Cr) is a trace element required for life. Biological activities of Cr are complicated and remain to be fully investigated. It is known that the valence of Cr plays an important role in the biological activities of Cr. For example, Cr (VI) is classified as a metal carcinogen [1], but Cr (III) is widely used as a nutritional supplement [2, 3]. Establishment of a gene expression profile for Cr-induced cellular response is necessary to facilitate investigation of the biological activities of Cr. In the present study, a large-scale gene expression analysis was conducted using RNA of human lung epithelial cells after in vitro exposure to Cr (VI). Utilizing high-density oligonucleotide arrays representing 2400 genes, we observed that expression of 150 genes was up-regulated, and that of 70 genes were down-regulated by Cr (VI). Functional analysis of these responsive genes led to an outline of potential biological activities of Cr in six aspects. The gene expression profile reveals that Cr may involves in redox stress, calcium mobilization, energy metabolism, protein synthesis, cell cycle regulation and carcinogenesis in the cell. The results provide a critical clue for understanding molecular mechanisms of the biological activities of Cr.
This paper references
Cloned transcription factor MTF‐1 activates the mouse metallothionein I promoter.
F. Radtke (1993)
Cadmium‐ and chromium‐induced oxidative stress, DNA damage, and apoptotic cell death in cultured human chronic myelogenous leukemic K562 cells, promyelocytic leukemic HL‐60 cells, and normal human peripheral blood mononuclear cells
D. Bagchi (2000)
ARE: Characterization of the retinoblastoma binding proteins
AR Fattaey (1993)
Nucleotide and deduced amino acid sequence of human threonyl-tRNA synthetase reveals extensive homology to the Escherichia coli and yeast enzymes.
M. Cruzen (1991)
Novel INK4 proteins, p19 and p18, are specific inhibitors of the cyclin D-dependent kinases CDK4 and CDK6.
H. Hirai (1995)
Chromium as an essential nutrient for humans.
R. Anderson (1997)
The alveolar macrophage as a model of calcium signaling in oxidative stress.
C. R. Hoyal (1998)
Multiple splicing variants of cdc25B regulate G2/M progression.
A. Forrest (1999)
Interaction of radicicol with members of the heat shock protein 90 family of molecular chaperones.
T. Schulte (1999)
Chromium(VI) treatment of normal human lung cells results in guanine-specific DNA polymerase arrest, DNA-DNA cross-links and S-phase blockade of cell cycle.
J. Xu (1996)
Positional cloning of the mouse obese gene and its human homologue
Y. Zhang (1995)
Complementary DNA for human T‐cell cyclophilin.
B. Haendler (1987)
Regulation of metallothionein gene expression.
G. Andrews (1990)
Cloning of a cDNA encoding a human homologue of CDC 47 , a member of the MCM fam
T Kiyono (1996)
Chromium-induced genotoxicity and apoptosis: relationship to chromium carcinogenesis (review).
J. Singh (1998)
Molecular cloning of the human casein kinase II alpha subunit.
H. Meisner (1989)
The p38 MAP kinase pathway signals for cytokine‐induced mRNA stabilization via MAP kinase‐activated protein kinase 2 and an AU‐rich region‐targeted mechanism
R. Winzen (1999)
Mutations in ATP2C1, encoding a calcium pump, cause Hailey-Hailey disease
Z. Hu (2000)
Milzman DP, Rodriguez A: Obesity
BR Boulanger (1994)
Oxidative mechanisms in the toxicity of metal ions.
S. Stohs (1995)
Nucleotide sequence of medium-chain acyl-CoA dehydrogenase mRNA and its expression in enzyme-deficient human tissue.
D. Kelly (1987)
Reduction of chromium(VI) and its relationship to carcinogenesis.
X. Shi (1999)
The role of oxygen free radicals in occupational and environmental lung diseases.
V. Vallyathan (1997)
p53‐inducible human homologue of Drosophila seven in absentia (Siah) inhibits cell growth: suppression by BAG‐1
S. Matsuzawa (1998)
Effects of chromium on body composition and weight loss.
R. Anderson (1998)
Effect of ubiquitin on platelet functions: possible identity with platelet activity suppressive lymphokine (PASL)
V. Pancré (1991)
Homologous ribosomal protein genes on the human X and Y chromosomes: Escape from X inactivation and possible implications for turner syndrome
E. Fisher (1990)
Activation of the human homologue of the Drosophila sina gene in apoptosis and tumor suppression.
M. Nemani (1996)
Gene expression profile of aging and its retardation by caloric restriction.
C. K. Lee (1999)
cyl encodes a putative cytoplasmic tyrosine kinase lacking the conserved tyrosine autophosphorylation site (Y416src).
J. Partanen (1991)
Light and dark in chromatin repair: repair of UV‐induced DNA lesions by photolyase and nucleotide excision repair
F. Thoma (1999)
Activation of the p38 Mitogen-activated Protein Kinase by Type I Interferons*
S. Uddin (1999)
A new member of the hsp90 family of molecular chaperones interacts with the retinoblastoma protein during mitosis and after heat shock.
C. Chen (1996)
Possible roles of nitric oxide and redox cell signaling in metal-induced toxicity and carcinogenesis: a review.
G. Buzard (2000)
Crosstalk between G protein-coupled receptors and tyrosine kinase signaling: Src take centre stage.
A. Ptasznik (2000)
Inhibition of T cell signaling by immunophilin-ligand complexes correlates with loss of calcineurin phosphatase activity.
J. Liu (1992)
Similarity Among the Drosophila (6-4)Photolyase, a Human Photolyase Homolog, and the DNA Photolyase-Blue-Light Photoreceptor Family
T. Todo (1996)
Chromium(VI)-induced nuclear factor-kappa B activation in intact cells via free radical reactions.
J. Ye (1995)
Molecular Cloning and Expression of the cDNAs Encoding Human and Yeast Mevalonate Pyrophosphate Decarboxylase (*)
M. J. Toth (1996)
Apoptosis is the mode of cell death caused by carcinogenic chromium.
L. Blankenship (1994)
Characterization of Multiple mRNAs That Encode Mammalian Translation Initiation Factor 5 (eIF-5)*
K. Si (1996)
Genetics of obesity and human energy metabolism.
C. Bouchard (1991)
Reactive oxygen-mediated protein oxidation in aging and disease.
E. Stadtman (1997)
Novel form of crosstalk between G protein and tyrosine kinase pathways.
M. Diversé-Pierluissi (1997)
Oxidative stress-inducible proteins in macrophages.
T. Ishii (1999)
Chromium in the prevention and control of diabetes.
R. Anderson (2000)
Molecular cloning of a novel non-receptor tyrosine kinase, HYL (hematopoietic consensus tyrosine-lacking kinase).
S. Sakano (1994)
ATF3 and stress responses.
T. Hai (1999)
Cloning of a cDNA encoding a human homologue of CDC47, a member of the MCM family.
T. Kiyono (1996)
Cloning of human calcineurin A: evidence for two isozymes and identification of a polyproline structural domain.
D. Guerini (1989)
Cloning and expression of cDNA for a human low-Km, rolipram-sensitive cyclic AMP phosphodiesterase.
G. P. Livi (1990)
Deficiency of a novel retinoblastoma binding protein 2-homolog is a consistent feature of sporadic human melanoma skin cancer.
T. Vogt (1999)
A novel pseudoautosomal gene encoding a putative GTP-binding protein resides in the vicinity of the Xp/Yp telomere.
F. Gianfrancesco (1998)
Role of Reactive Oxygen Species and p53 in Chromium(VI)-induced Apoptosis*
J. Ye (1999)
hCDC47, a Human Member of the MCM Family
M. Fujita (1996)
The cdc25B phosphatase is essential for the G2/M phase transition in human cells.
C. Lammer (1998)
Chromium chloride induces chromosomal aberrations in human lymphocytes via indirect action.
J. Friedman (1987)
The role of chromium in nutrition and therapeutics and as a potential toxin.
K. Jeejeebhoy (1999)
Char - acterization of the retinoblastoma binding proteins RBP 1 and RBP 2
AR Fattaey (1993)
Isolation and characterization of p19INK4d, a p16-related inhibitor specific to CDK6 and CDK4.
K. Guan (1996)
Extensive polymorphisms observed in HIV–1 clade B protease gene using high–density oligonucleotide arrays
M. Kozal (1996)
Cadmium suppresses apoptosis induced by chromium.
H. Shimada (1998)
Genomic Structure and Promoter Analysis of the Human obese Gene (*)
D. W. Gong (1996)
Chromium(VI) Induces p53‐Dependent Apoptosis in Diploid Human Lung and Mouse Dermal Fibroblasts
D. Carlisle (2000)
Extension of life-span by overexpression of superoxide dismutase and catalase in Drosophila melanogaster.
W. C. Orr (1994)
CDC25 phosphatases as potential human oncogenes.
K. Galaktionov (1995)

This paper is referenced by
Chromium(VI) causes down regulation of biotinidase in human bronchial epithelial cells by modifications of histone acetylation.
B. Xia (2011)
The role of protein kinase CK2 in metal ion toxicity
A. J. Johnson (2018)
Gene expression profiling and bioinformatics analysis in 16HBE cells treated by chromium (VI).
Guiping Hu (2016)
Highlight Article: Chromium exposure disrupts chromatin architecture upsetting the mechanisms that regulate transcription
Hesbon A Zablon (2019)
Review of transcriptomic responses to hexavalent chromium exposure in lung cells supports a role of epigenetic mediators in carcinogenesis.
J. Rager (2019)
High‐Valent Cr, Mn, and Fe Species
Virender K. Sharma (2012)
Cytotoxicity and genome-wide microarray analysis of intestinal smooth muscle cells in response to hexavalent chromium induction.
Lifang Jin (2013)
Impact of Carcinogenic Chromium on the Cellular Response to Proteotoxic Stress
Leonardo M. R. Ferreira (2019)
Cytogenomics of hexavalent chromium (Cr6+) exposed cells: A comprehensive review
Akanksha Nigam (2014)
Comparison of Gene Expression Profiles in Chromate Transformed BEAS-2B Cells
H. Sun (2011)
Hexavalent chromium-induced alteration of proteomic landscape in human skin fibroblast cells.
Lei Guo (2013)
Hexavalent chromium induced ROS formation, Akt, NF-kappaB, and MAPK activation, and TNF-alpha and IL-1alpha production in keratinocytes.
Bour-Jr Wang (2010)
Molecular characterization of chromium (VI) reducing potential in Gram positive bacteria isolated from contaminated sites
R. Patra (2010)
The New Role for an Old Kinase: Protein Kinase CK2 Regulates Metal Ion Transport
A. J. Johnson (2016)
Detoxification hexavalent chromium by potential chromate reducing bacteria isolated from turnery effluent
Sudhanshu Shekhar (2014)
Związki chromu(VI) – w przeliczeniu na Cr(VI). Dokumentacja proponowanych dopuszczalnych wielkości narażenia zawodowego
J. Skowroń (2016)
Molecular mechanisms of Cr(VI)-induced carcinogenesis
M. Ding (2004)
Carcinogenicity of hexavalent chromium.
A. Holmes (2008)
Metal-induced toxicity, carcinogenesis, mechanisms and cellular responses
S. Leonard (2004)
Analysis of Immune-Relevant Genes Expressed in Spleen of Capra hircus Kids Fed with Trivalent Chromium
M. Sadeghi (2013)
Modulation of histone methylation and MLH1 gene silencing by hexavalent chromium.
H. Sun (2009)
Impact of hexavalent chromium on mammalian cell bioenergetics: phenotypic changes, molecular basis and potential relevance to chromate-induced lung cancer
P. Abreu (2014)
Toxicogenomic Approach to Risk Assessment of Welding Fumes
Kyung Taek Rim (2012)
Involvement of Calcium, Reactive Oxygen Species, and ATP in Hexavalent Chromium-Induced Damage in Red Blood Cells
R. Zhang (2014)
Gene-environment interactions in heavy metal and pesticide carcinogenesis.
S. Tabrez (2014)
Generation of reactive oxygen species in the enzymatic reduction of PbCrO4 and related DNA damage
S. Leonard (2002)
Influencia del estrés ocupacional en el proceso salud- enfermedad
G. L. Mompó (2003)
Toxicogenomic approaches for understanding molecular mechanisms of heavy metal mutagenicity and carcinogenicity.
Preeyaporn Koedrith (2013)
Transcriptomics Evaluation of Hexavalent Chromium Toxicity in Human Dermal Fibroblasts
R. Sellamuthu (2011)
Changes in protein expression associated with chronic in vitro exposure of hexavalent chromium to osteoblasts and monocytes: a proteomic approach.
V. Raghunathan (2010)
PbCrO4 mediates cellular responses via reactive oxygen species
S. Leonard (2004)
Chromium IV exposure, via Src/Ras signaling, promotes cell transformation
S. Ganapathy (2017)
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