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Expression Profiles Of The Arabidopsis WRKY Gene Superfamily During Plant Defense Response

J. Dong, C. Chen, Z. Chen
Published 2004 · Biology, Medicine

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WRKY proteins are a recently identified class of DNA-binding proteins that recognize the TTGAC(C/T) W-box elements found in the promoters of a large number of plant defense-related genes. With oligo molecules containing the W-box sequences as probes, we detected a number of WRKY DNA-binding activities in Arabidopsis that were induced by salicylic acid (SA). Search of the Arabidopsis genome identifies 72 genes encoding proteins characteristic of WRKY DNA-binding transcription factors that can be divided into three groups based on the number and structures of their WRKY zinc-finger motifs. Northern blotting analysis revealed that 49 of the 72 AtWRKY genes were differentially regulated in the plants infected by an avirulent strain of the bacterial pathogen Pseudomonas syringae or treated by SA. These pathogen- and/or SA-regulated WRKY genes can be further categorized into groups based on their expression patterns in both wild-type plants and mutants defective in defense signaling pathways. Inspection of the 5′ sequences upstream of the predicated translation start sites revealed a substantial enrichment of W boxes in the promoters of pathogen- and/or SA-regulated Arabidopsis WRKY genes. These results suggest that defense-regulated expression of WRKY genes involves extensive transcriptional activation and repression by its own members of the transcription factor superfamily.
This paper references
Signal perception and transduction in plant defense responses.
Y. Yang (1997)
A pathogen- and salicylic acid-induced WRKY DNA-binding activity recognizes the elicitor response element of the tobacco class I chitinase gene promoter
P. Yang (1999)
The Arabidopsis NPR1 Gene That Controls Systemic Acquired Resistance Encodes a Novel Protein Containing Ankyrin Repeats
H. Cao (1997)
Transcriptional control of plant genes responsive to pathogens.
P. Rushton (1998)
Identification of rice blast fungal elicitor-responsive genes by differential display analysis.
C. Y. Kim (2000)
Molecular cloning: A laboratory manual 2nd ed. Edited by J. . Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989 (in 3 volumes)
P. Deininger (1990)
The WRKY superfamily of plant transcription factors.
T. Eulgem (2000)
Members of a new family of DNA-binding proteins bind to a conserved cis-element in the promoters of α-Amy2 genes
P. Rushton (2004)
Evidence for an Important Role of WRKY DNA Binding Proteins in the Regulation of NPR1 Gene Expression
D. Yu (2001)
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
Early nuclear events in plant defence signalling: rapid gene activation by WRKY transcription factors
T. Eulgem (1999)
Requirement of Salicylic Acid for the Induction of Systemic Acquired Resistance
T. Gaffney (1993)
Towards functional characterisation of the members of the R2R3-MYB gene family from Arabidopsis thaliana.
H. Kranz (1998)
Retinoid X receptor and its partners in the nuclear receptor family.
F. Rastinejad (2001)
In planta Agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration.
N. Bechtold (1998)
Interaction of elicitor‐induced DNA‐binding proteins with elicitor response elements in the promoters of parsley PR1 genes.
P. Rushton (1996)
Is the High Basal Level of Salicylic Acid Important for Disease Resistance in Potato?
D. Yu (1997)
Identification of genes encoding receptor-like protein kinases as possible targets of pathogen- and salicylic acid-induced WRKY DNA-binding proteins in Arabidopsis.
L. Du (2000)
Sequence‐specific interactions of a pea nuclear factor with light‐responsive elements upstream of the rbcS‐3A gene.
P. Green (1987)
The transcriptome of Arabidopsis thaliana during systemic acquired resistance
K. Maleck (2000)
Rapid systemic accumulation of transcripts encoding a tobacco WRKY transcription factor upon wounding
K. Hara (2000)
RPS2 of Arabidopsis thaliana: a leucine-rich repeat class of plant disease resistance genes.
A. Bent (1994)
Characterization of a cDNA encoding a novel DNA-binding protein, SPF1, that recognizes SP8 sequences in the 5′ upstream regions of genes coding for sporamin and β-amylase from sweet potato
S. Ishiguro (2004)
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
J. Thompson (1997)
An oligo selection procedure for identification of sequence-specific DNA-binding activities associated with the plant defence response.
Z. Wang (1998)
Is the high basal levels of salicylic acid important for disease resistance in potato? Plant Physiol
D. Yu (1997)
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
J. Thompson (1994)
Isolation and characterization of two pathogen- and salicylic acid-induced genes encoding WRKY DNA-binding proteins from tobacco
C. Chen (2004)
Analysis of the genome sequence of the flowering plant Arabidopsis thaliana
The Arabidopsis Genome Initiative (2000)
A Central Role of Salicylic Acid in Plant Disease Resistance
T. Delaney (1994)
Cluster analysis and display of genome-wide expression patterns.
M. Eisen (1998)
Characterization of a zinc-dependent transcriptional activator from Arabidopsis.
S. de Pater (1996)
Nuclear-receptor interactions on DNA-response elements.
S. Khorasanizadeh (2001)
A potato gene encoding a WRKY-like transcription factor is induced in interactions with Erwinia carotovora subsp. atroseptica and Phytophthora infestans and is coregulated with class I endochitinase expression.
A. Dellagi (2000)
TreeView: an application to display phylogenetic trees on personal computers
R. Page (1996)

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Genome Wide Analysis of Nucleotide-Binding Site Disease Resistance Genes in Brachypodium distachyon
Shenglong Tan (2012)
Glutamate fermentation by-product activates plant defence responses and confers resistance against pathogen infection.
D. Igarashi (2010)
The promoter of a gene encoding an isoflavone reductase-like protein in coffee (Coffea arabica) drives a stress-responsive expression in leaves
M. Brandalise (2009)
Transcription factors as tools to engineer enhanced drought stress tolerance in plants
S. S. Hussain (2011)
Using cell type specific transcriptomics to understand how Arabidopsis roots respond to Sinorhizobium meliloti.
Jo Hulsmans (2014)
WRKY gene family evolution in Arabidopsis thaliana
Q. Wang (2011)
The Tall Fescue Turf Grass Class I Chitinase Gene FaChit1 Is Activated by Fungal Elicitors, Dehydration, Ethylene, and Mechanical Wounding
J. Wang (2008)
Plant Body Weight-Induced Secondary Growth in Arabidopsis and Its Transcription Phenotype Revealed by Whole-Transcriptome Profiling1[w]
Jae-Heung Ko (2004)
Transcriptional profiling by cDNA-AFLP and microarray analysis reveals novel insights into the early response to ethylene in Arabidopsis.
A. De Paepe (2004)
Transcription Factors Regulating Plant Defense Responses
P. Fobert (2006)
Molecular–genetic aspects of plant immunity to phytopathogenic bacteria and fungi
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Molecular cloning and expression analysis of WRKY transcription factor genes in Salvia miltiorrhiza
C. Li (2015)
Large-scale transcriptome analysis reveals arabidopsis metabolic pathways are frequently influenced by different pathogens
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HbWRKY40 plays an important role in the regulation of pathogen resistance in Hevea brasiliensis
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