Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Effect Of Chlorophyll-Protein Complex I Deficiency On The Physiological Character Of A Chlamydomonas Reinhardtii Mutant

Qing-Xiu Tang, Zhang-Lin Ni, Jia-mian Wei
Published 2004 · Biology

Save to my Library
Download PDF
Analyze on Scholarcy
Share
In the mutant CC-1047 of Chlamydomonas reinhardtii, LDS-PAGE showed that the chlorophyll-protein complex I (CPI) is almost absent. The mutant could not grow in a culture medium without organic carbon source while the wild type (WT) C. reinhardtii grew quickly. When an organic carbon source was added into the culture medium, the mutant grew almost as well as WT. The rate of photosystem 1 (PS1) electron transport (DCPIP→MV) and the rate of whole chain electron transport (H2O→MV) of chloroplasts of the CC-1047 mutant were both lower than those of WT. The photophosphorylation activity, photosynthetic O2 evolution rate, and rate of NADP+ photoreduction of CC-1047 were also much lower than the activities of WT. There were some differences in ATPase activity between the mutant and WT. Two different activation ways were used to activate the latent ATPase using methanol and dithiothreitol (DTT) as activation substrate. More methanol and DTT were required for the mutant than WT to obtain the maximum activity. Thus the photosynthetic apparatus could not operate normally when CPI was absent because of the abnormal PS1 electron transport. Meanwhile, the other adjacent complexes of the thylakoid membrane, for example, ATP synthase complex, were slightly affected.
This paper references
10.1002/j.1460-2075.1991.tb07733.x
Directed chloroplast transformation in Chlamydomonas reinhardtii: insertional inactivation of the psaC gene encoding the iron sulfur protein destabilizes photosystem I.
Y. Takahashi (1991)
10.1016/S0005-2728(05)80184-7
Nigericin and hexylamine effects on localized proton gradients in thylakoids
F. Allnutt (1991)
10.1104/PP.24.1.1
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.
D. Arnon (1949)
10.1007/0-306-48127-8_25
The Regulation of Chloroplast ATP Synthase, CF0-CF1
J. D. Mills (1996)
10.1021/BI00522A020
Isolation, purification, and characterization of coupling factor 1 from Chlamydomonas reinhardi.
S. Selman-Reimer (1981)
10.1007/BF00018295
Characterization of a Synechococcus sp. strain PCC 7002 mutant lacking Photosystem I. Protein assembly and energy distribution in the absence of the Photosystem I reaction center core complex
G. Shen (2004)
10.1016/S0014-5793(97)01170-8
Limited photosynthetic electron flow but no CO2 fixation in Chlamydomonas mutants lacking photosystem I
L. Cournac (1997)
10.1007/978-3-642-70936-4_3
Biochemical Characterization and Structure of Pigment-Proteins of Photosynthetic Organism
J. Thornber (1986)
10.1002/j.1460-2075.1995.tb00180.x
Isolation of a psaF‐deficient mutant of Chlamydomonas reinhardtii: efficient interaction of plastocyanin with the photosystem I reaction center is mediated by the PsaF subunit.
J. Farah (1995)
10.1146/ANNUREV.PP.26.060175.001015
Chlorophyll-Proteins: Light-Harvesting and Reaction Center Components of Plants
J. Thornber (1975)
10.1111/j.1751-1097.1976.tb07259.x
HETEROGENEITY OF THE PHOTOCHEMICAL CENTERS IN SYSTEM II OF CHLOROPLASTS *
A. Melis (1976)
10.1016/S0005-2728(98)00136-4
Chlamydomonas genetics, a tool for the study of bioenergetic pathways.
M. Hippler (1998)
10.1016/0926-6585(66)90323-2
Two pigment proteins in spinach chloroplasts.
T. Ogawa (1966)
10.1007/BF00435689
Studies on mutants deficient in the photosystem I reaction centers in Chlamydomonas reinhardtii
J. Girard (2004)
10.1016/0005-2728(88)90121-1
Azide inhibition of chloroplast ATPase is prevented by a high protonmotive force
Jia-mian Wei (1988)
10.1126/science.273.5273.364
Oxygenic Photoautotrophic Growth Without Photosystem I
J. W. Lee (1996)
10.1016/0005-2728(82)90149-9
A new chlorophyll-protein complex related to Photosystem I in Chlamydomonas reinhardii
F. Wollman (1982)
10.1016/s0021-9258(17)39868-x
Role of a disulfide bond in the gamma subunit in activation of the ATPase of chloroplast coupling factor 1.
C. Nalin (1984)
10.1021/BI00854A004
Studies on the nature of the chloroplast lamella. I. Preparation and some properties of two chlorophyll-protein complexes.
Thornber Jp (1967)
10.1016/S0076-6879(80)69024-7
[22] Ferredoxin-NADP+ oxidoreductase
G. Zanetti (1980)
Stimulatory Effects of Polymixin B on Photophosphorylation
W. Jia (1990)
10.1016/s0021-9258(18)31484-4
Site-directed mutagenesis of the photosystem I reaction center in chloroplasts. The proline-cysteine motif.
A. Webber (1993)
10.1074/jbc.274.15.10466
Photosystem I Is Indispensable for Photoautotrophic Growth, CO2 Fixation, and H2 Photoproduction inChlamydomonas reinhardtii *
K. Redding (1999)
10.1016/s0021-9258(18)66180-0
A microcolorimetric method for the determination of inorganic phosphorus.
H. Taussky (1953)
10.1016/s0021-9258(19)52546-7
Electrophoretic purification of chlorophyll a/b-protein complexes from Chlamydomonas reinhardtii and spinach and analysis of their polypeptide compositions.
P. Delepelaire (1981)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar