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Magnetic Beads (Dynabead™) Toxicity To Endothelial Cells At High Bead Concentration: Implication For Tissue Engineering Of Vascular Prosthesis

A. Tiwari, G. Punshon, A. Kidane, G. Hamilton, A. Seifalian
Published 2004 · Medicine, Biology

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Magnetic beads (Dynabeads™) have been used for the purification of endothelial cells. One application for this procedure may be for single-stage seeding of bypass grafts. The number of endothelial cells (EC) isolated is crucial and therefore to increase the number of cells extracted, a higher number of Dynabeads™ per cell may need to be used. The effect of large numbers of CD31 Dynabeads™ on cell proliferation/metabolism is unknown. We undertook this study using CD31-coated Dynabeads™ and EC from human umbilical vein. EC were coated at concentrations of 4, 10, or 50 beads per cell. The cells were cultured for 6 days with control being normal EC. Cellular proliferation was assessed by trypsinization of cells and metabolism assessed with an Alamar blue™ viability assay. In a further experiment a compliant polyurethane graft was single-stage seeded with both coated Dynabeads™ and normal EC. The results showed that using a higher number of beads per cell resulted in a reduction in cell proliferation and a reduction in cell metabolism. The total number of Dynabeads™-coated cells in culture compared to controls (%) by day 6 were 30.7±2.56, 41.3±9.8 and 59.2±7.3 for 50, 10, and 4 beads per cell, respectively. The corresponding results for Alamar blue were 43.7±1.2, 61.8±1.4, and 72.1±4.3. The seeded grafts showed reduced metabolism with the Dynabeads™-coated EC. In conclusion, high numbers of beads per cell have a late detrimental effect on cell proliferation and metabolism. Therefore for single-stage seeding lower numbers of Dynabeads™ will need to be used with resultant reduction in the number of available EC.
This paper references
A new technique for measuring the cell growth and metabolism of endothelial cells seeded on vascular prostheses.
A. Seifalian (2001)
Tissue engineering of vascular bypass grafts: role of endothelial cell extraction.
A. Tiwari (2001)
Improving the clinical patency of prosthetic vascular and coronary bypass grafts: the role of seeding and tissue engineering.
A. Seifalian (2002)
Improving endothelial cell retention for single stage seeding of prosthetic grafts: use of polymer sequences of arginine-glycine-aspartate.
A. Tiwari (2003)
Effect of prolonged pulsatile shear stress in vitro on endothelial cell seeded PTFE and compliant polyurethane vascular grafts.
A. Giudiceandrea (1998)
Optimal endothelialisation of a new compliant poly(carbonate-urea)urethane vascular graft with effect of physiological shear stress.
H. Salacinski (2000)
Development of a hybrid cardiovascular graft using a tissue engineering approach 1
A. Tiwari (2002)
Clinical experience with autologous endothelial cell-seeded polytetrafluoroethylene coronary artery bypass grafts.
H. Laube (2000)
In vitro stability of a novel compliant poly(carbonate-urea)urethane to oxidative and hydrolytic stress.
H. Salacinski (2002)
Electron microscopic and immunocytochemical profiles of human subcutaneous fat tissue microvascular endothelial cells
M. Vici (1993)
Clinical autologous in vitro endothelialization of infrainguinal ePTFE grafts in 100 patients: a 9-year experience.
M. Deutsch (1999)
Kinetics of endothelial cell seeding.
J. E. Rosenman (1985)
Multilineage cells from human adipose tissue: implications for cell-based therapies.
P. Zuk (2001)
Binding of human endothelium to Ulex europaeus I-coated Dynabeads: application to the isolation of microvascular endothelium.
C. Jackson (1990)
Evaluation of Expanded Polytetrafluoroethylene Arteriovenous Access Grafts onto which Microvessel-Derived Cells were Transplanted to ``Improve'' Graft Performance: Preliminary Results
S. Schmidt (1998)
Cellular engineering of vascular bypass grafts: Role of chemical coatings for enhancing endothelial cell attachment
H. Salacinski (2006)
Tissue factor expression by cells used for sodding of prosthetic vascular grafts.
F. Rubens (1997)
Improving the patency of vascular bypass grafts: the role of suture materials and surgical techniques on reducing anastomotic compliance mismatch.
A. Tiwari (2003)
Contaminants from the transplant contribute to intimal hyperplasia associated with microvascular endothelial cell seeding.
C. H. Arts (2002)
Immunomagnetic purification of human microvessel endothelial cells using Dynabeads coated with monoclonal antibodies to PECAM-1.
P. Hewett (1993)
Optimization of use of UEA-1 magnetic beads for endothelial cell isolation
V. Conrad-Lapostolle (2004)

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M. Gijs (2004)
Magnetically Seeded Bioreactor for Three-Dimensional Cell Culture
A. W. Chin (2010)
Accelerating in Situ Endothelialisation of Cardiovascular Bypass Grafts
Ee Teng Goh (2014)
Improving the hemocompatibility of biomedical composites
G. Voskerician (2018)
Zellkulturverfahren und kultiviertes gewebe
H. Honda (2004)
Advances in Cell Seeding of Tissue Engineered Vascular Grafts
Justin S. Weinbaum (2020)
The isolation, characterisation and investigation into the in vitro behaviour of human ocular vascular endothelial cells
Andrew C. Browning (2013)
Mechanotransduction Effects on Endothelial Cell Proliferation via CD31 and VEGFR2: Implications for Immunomagnetic Separation.
K. Mahajan (2017)
One-step separation of CD20 + cells from whole blood using bacterial magnetic particles displaying protein G
M. Takahashi (2008)
Fabrication and characterization of multimodal magnetic - fluorescent polystyrene nanowires as selective cell imaging probes
J. Mccarthy (2011)
Development of an improved two-stage seeding process for a nanocomposite vascular graft using human peripheral-blood derived endothelial cells
G. Punshon (2017)
Fundamentals and application of magnetic particles in cell isolation and enrichment: a review.
Brian D. Plouffe (2015)
Synthesis of Modified Hydroxyapatite (HAP) Substituted With Fe Ion for DDS Application
A. Nakahira (2007)
Cardiovascular tissue engineering: state of the art Ingenierie tissulaire appliqué aux vaisseaux sanguins : état de l'art
D. Vara (2005)
Engineered Fibrin Scaffolds for Cardiac Tissue Repair
K. S. Thomson (2013)
Cell-seeding techniques in vascular tissue engineering.
G. Villalona (2010)
Biomaterials and Tissue Engineering Micropatterned cell co-cultures using layer-by-layer deposition of extracellular matrix components
J. Fukuda (2005)
Molecular analysis of magnetotactic bacteria and development of functional bacterial magnetic particles for nano-biotechnology.
T. Matsunaga (2007)
Electrochemical desorption of self-assembled monolayers for engineering cellular tissues.
R. Inaba (2009)
Magnetic reconstruction of three-dimensional tissues from multicellular spheroids.
Ruei-Zeng Lin (2008)
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K. Liburdi (2012)
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D. Radke (2018)
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