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Detoxification Of The Plant Toxin Fluoroacetate By A Genetically Modified Rumen Bacterium

K. Gregg, C. L. Cooper, D. Schafer, H. Sharpe, C. Beard, G. Allen, Jianwei Xu
Published 1994 · Biology, Medicine

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We isolated the fluoroacetate dehalogenase gene (H1), from Moraxella species strain B, and placed it under the transcriptional control of a 154 bp fragment of the erm gene promoter. The promoter/gene construct was attached to the Butyrivibrio fibrisolvens shuttle vector pBHerm, and the resulting dehalogenase expression plasmid (pBHf) was transferred to B, fibrisolvens OB156 by electroporation. The erm gene promoter directed expression of dehalogenase activity in both E. coli and B. fibrisolvens OB156. Cell–free lysates of the genetically modified OB156 defluorinated 10.6 nmol fluoroacetate/min/mg protein. Growing cultures of OB156 were able to detoxify fluoroacetate in the culture medium, at the rate of 9.9 nmol/min/mg. Plasmid pBHf was retained by 100% of OB156 cells after 500 generations of non–selective culture. The restriction pattern of pBHf remained unchanged after extensive non–selective growth and host bacteria continued to produce active dehalogenase. The construction of rumen bacteria that are able to detoxify an important natural poison supports the feasibility of using genetically modified rumen bacteria to aid animal production.
This paper references
10.1038/201827A0
Isolation and Identification of the Toxic Principle of Gastrolobium grandiflorum
T. Mcewan (1964)
10.1038/227680A0
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
U. K. Laemmli (1970)
Poison plants of Western Australia : the toxic species of the genus Gastrolobium and Oxylobium.
T. Aplin (1973)
10.1071/ZO9790363
Fluoroacetate Tolerance, a Genetic Marker in Some Australian Mammals.
A. Oliver (1979)
10.1271/BBB1961.45.35
Purification and Properties of Haloacetate Halidohydrolase Specified by Plasmid from Moraxella sp. Strain B
H. Kawasaki (1981)
10.1271/BBB1961.45.29
Plasmid-determined dehalogenation of haloacetates in Moraxella species.
H. Kawasaki (1981)
10.3168/JDS.S0022-0302(83)81970-5
Prospects for development and use of recombinant deoxyribonucleic acid techniques with ruminal bacteria.
C. J. Smith (1983)
10.1071/WR9830507
The Sensitivity of Australian Animals to 1080 Poison VI.* Bandicoots
J. Mcilroy (1983)
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
10.1271/BBB1961.48.2627
Cloning and Expression in Escherichia coli of the Haloacetate Dehalogenase Genes from Moraxella Plasmid pUO1
H. Kawasaki (1984)
10.1128/JB.157.2.445-453.1984
Physical and genetic analyses of streptococcal plasmid pAM beta 1 and cloning of its replication region.
D. Leblanc (1984)
10.1071/WR9840373
The Sensitivity of Australian Animals to 1080 Poison. Vii. Native and Introduced Birds.
J. Mcilroy (1984)
10.4141/CJAS85-067
APPLICATION OF GENE MANIPULATION TO RUMEN MICROFLORA
R. Teather (1985)
10.1111/J.1751-0813.1986.TB02990.X
Successful transfer of DHP-degrading bacteria from Hawaiian goats to Australian ruminants to overcome the toxicity of Leucaena.
R. J. Jones (1986)
10.1016/0003-2697(87)90021-2
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
P. Chomczyński (1987)
10.1093/NAR/16.4.1563
McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning.
E. Raleigh (1988)
10.1071/WR9880239
Tolerance to, and metabolism of, fluoroacetate in the Emu
L. E. Twigg (1988)
10.1093/nar/16.13.6127
High efficiency transformation of E. coli by high voltage electroporation
W. Dower (1988)
10.1007/978-94-011-9702-1_30
Engineering Ruminal Flora for Improved Protein Quality
J. Brooker (1988)
10.1099/00221287-135-9-2543
Isolation of an endoglucanase gene from Bacteroides ruminicola subsp. brevis.
J. R. Woods (1989)
10.1093/nar/18.13.3983
Constructing DNA by polymerase recombination
A. Yolov (1990)
10.1016/B978-0-12-702290-1.50036-9
Enhancement of Rumen Microbial Detoxification by Gene Transfer
K. Gregg (1991)
10.1128/aem.57.1.277-282.1991
Introduction of the Bacteroides ruminicola xylanase gene into the Bacteroides thetaiotaomicron chromosome for production of xylanase activity
T. Whitehead (1991)
10.1111/j.1472-765X.1992.tb00759.x
Genetic transformation of the ruminal bacteria Butyrivibrio fibrisolvens and Streptococcus bovis by electroporation
T.R. Whitehead (1992)
10.1099/00221287-138-7-1317
Lack of homology between two haloacetate dehalogenase genes encoded on a plasmid from Moraxella sp. strain B.
H. Kawasaki (1992)
Practical genetic engineering of human bacteria
K. Gregg (1993)



This paper is referenced by
10.3390/pr8121633
Recombinant Technologies to Improve Ruminant Production Systems: The Past, Present and Future
A. Pech-Cervantes (2020)
10.3390/toxins12100664
Toxin Degradation by Rumen Microorganisms: A Review
Zhi Hung Loh (2020)
10.1007/978-3-030-21309-1_7
Designer Probiotics: The Next-Gen High Efficiency Biotherapeutics
Birbal Singh (2019)
10.1016/j.toxicon.2019.02.005
Sequential administrations of trifluoroacetate induce tolerance to Palicourea maccgravii, a monofluoroacetate‐containing plant, in calves
A. G. Costa (2019)
10.22456/1679-9216.89907
Detection and Characterization of Bovine Rumen Microorganisms Resistant to Sodium Fluoroacetate
M. F. Pimentel (2019)
10.1007/978-3-319-71950-4_1
Probiotics and Prebiotics: An Overview on Recent Trends
G. Zoumpopoulou (2018)
10.1186/s40104-017-0180-6
Fluoroacetate in plants - a review of its distribution, toxicity to livestock and microbial detoxification
L. Leong (2017)
10.1080/07388551.2016.1267109
Natural production of fluorinated compounds and biotechnological prospects of the fluorinase enzyme
M. Carvalho (2017)
10.2527/jas2017.1731
Ground Juniperus pinchotii and urea in supplements fed to Rambouillet ewe lambs Part 2: Ewe lamb rumen microbial communities.
S. L. Ishaq (2017)
A inoculação ruminal de Enterococcus faecalis é eficiente no controle da intoxicação por monofluoroacetato de sódio em ovinos
Geovanny Bruno Gonçalves Dias (2015)
10.1146/annurev-animal-022114-110705
The early impact of genomics and metagenomics on ruminal microbiology.
S. Denman (2015)
10.1590/S0100-736X2014000700007
Administração repetida de doses não tóxicas de monofluoroacetato de sódio não protege contra a intoxicação por este composto em ovinos
Ariany Carvalho dos Santos (2014)
10.1021/jf500563h
Monofluoroacetate-containing plants that are potentially toxic to livestock.
S. Lee (2014)
10.1111/EMR.12071
Biodegradation of 1080: testing soils in south-eastern Australia for sodium fluoroacetate-degrading micro-organisms.
M. Gentle (2014)
10.1371/journal.pone.0083647
Fermentation Products of Solvent Tolerant Marine Bacterium Moraxella spp. MB1 and Its Biotechnological Applications in Salicylic Acid Bioconversion
S. Wahidullah (2013)
E COMMISSION ON GENETIC RESOURCES FOR FOOD AND AGRICULTURE MICRO-ORGANISMS AND RUMINANT DIGESTION : STATE OF KNOWLEDGE , TRENDS AND FUTURE PROSPECTS
C. McSweeney (2012)
10.1590/S0100-736X2012000400008
Efeito protetor da acetamida em bovinos indica monofluoroacetato como princípio tóxico de Palicourea marcgravii (Rubiaceae)
T. C. Peixoto (2012)
10.1111/j.1574-6941.2012.01338.x
Isolation and survey of novel fluoroacetate-degrading bacteria belonging to the phylum Synergistetes.
C. Davis (2012)
10.1100/2012/149893
Defluorination of Sodium Fluoroacetate by Bacteria from Soil and Plants in Brazil
Expedito K. A. Camboim (2012)
10.1024/0300-9831/a000098
In vitro conversion of ß-carotene to retinal in bovine rumen fluid by a recombinant ß-carotene- 15, 15'-monooxygenase.
Esperanza García-López (2012)
Commission on Genetic Resources for Food and Agriculture. Micro-organisms and ruminant digestion: State of knowledge, trends and future prospects
C. McSweeney (2012)
10.1038/nbt.1615
High-resolution DNA analysis of human embryonic stem cell lines reveals culture-induced copy number changes and loss of heterozygosity
Elisa Närvä (2010)
Comparative analysis of two attachment variants of butyrivibrio fibrisolvens.
H. Seno (2010)
10.1590/S1516-35982009001300034
Recent developments in nucleic acid based techniques for use in rumen manipulation
C. McSweeney (2009)
10.1099/ijs.0.002915-0
Bifidobacterium bombi sp. nov., from the bumblebee digestive tract.
J. Killer (2009)
10.1007/BF02825883
Phenotypic and genetic data supporting reclassification ofButyrivibrio fibrisolvens isolates
J. Kopečný (2008)
10.1007/BF02818798
Identification and characterization ofClostridium paraputrificum, a chitinolytic bacterium of human digestive tract
J. Šimůnek (2008)
10.1099/MIC.0.2007/006502-0
Isolation and characterization of putative Pseudobutyrivibrio ruminis promoters.
Tobias D. Schoep (2007)
10.1080/10408440601177863
A Review of the Hepatotoxic Plant Lantana camara
O. Sharma (2007)
10.1016/S1877-1823(09)70106-7
Manipulation and characterization of the rumen ecosystem through biotechnology.
T. McAllister (2006)
10.1007/0-387-30744-3_35
The Family Lachnospiraceae, Including the Genera Butyrivibrio, Lachnospira and Roseburia
M. Cotta (2006)
10.1016/J.JFLUCHEM.2004.12.001
Microbial cleavage of CF bond
R. Natarajan (2005)
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