Online citations, reference lists, and bibliographies.
← Back to Search

The IgE-reactive Autoantigen Hom S 2 Induces Damage Of Respiratory Epithelial Cells And Keratinocytes Via Induction Of IFN-gamma.

I. Mittermann, R. Reininger, M. Zimmermann, K. Gangl, J. Reisinger, K. Aichberger, Elli K. Greisenegger, V. Niederberger, J. Seipelt, B. Bohle, T. Kopp, C. Akdis, S. Spitzauer, P. Valent, R. Valenta
Published 2008 · Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
Hom s 2, the alpha-chain of the nascent polypeptide-associated complex, is an intracellular autoantigen that has been identified with IgE autoantibodies from atopic dermatitis patients. We investigated the humoral and cellular immune response to purified recombinant Hom s 2 (rHom s 2). rHom s 2 exhibited IgE reactivity comparable to exogenous allergens, but did not induce relevant basophil cell degranulation. The latter may be attributed to the fact that patients recognized single epitopes on Hom s 2 as revealed by IgE epitope mapping with rHom s 2 fragments. In contrast to exogenous allergens, rHom s 2 had the intrinsic ability to induce the release of IFN-gamma in cultured peripheral blood mononuclear cells from atopic as well as non-atopic individuals. IFN-gamma-containing culture supernatants from Hom s 2-stimulated peripheral blood mononuclear cells caused disintegration of respiratory epithelial cell layers and apoptosis of skin keratinocytes, which could be inhibited with a neutralizing anti-IFN-gamma antibody. Our data demonstrate that the Hom s 2 autoantigen can cause IFN-gamma-mediated cell damage.
This paper references
10.1073/PNAS.76.9.4350
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
H. Towbin (1979)
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
10.1016/0003-2697(86)90228-9
Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea.
S. Fling (1986)
10.1073/PNAS.86.14.5542
Interleukin 3 activates human blood basophils via high-affinity binding sites.
P. Valent (1989)
10.1126/SCIENCE.1857985
Identification of profilin as a novel pollen allergen; IgE autoreactivity in sensitized individuals.
R. Valenta (1991)
10.1084/JEM.175.2.377
Profilins constitute a novel family of functional plant pan-allergens
R. Valenta (1992)
10.1016/s0021-9258(18)46931-1
Isolation of an immunodominant IgE hapten from an epitope expression cDNA library. Dissection of the allergic effector reaction.
T. Ball (1994)
10.1165/AJRCMB.10.1.7507342
CFTR expression and chloride secretion in polarized immortal human bronchial epithelial cells.
A. Cozens (1994)
10.1016/S0140-6736(94)90879-6
Lesional expression of interferon-γ in atopic eczema
M. Grewe (1994)
10.1111/1523-1747.EP12321078
Analysis of the cytokine pattern expressed in situ in inhalant allergen patch test reactions of atopic dermatitis patients.
M. Grewe (1995)
10.1084/JEM.184.1.265
Humoral and cell-mediated autoimmunity in allergy to Aspergillus fumigatus
R. Crameri (1996)
10.1111/1523-1747.EP12331164
Allergen specificity of skin-infiltrating T cells is not restricted to a type-2 cytokine pattern in chronic skin lesions of atopic dermatitis.
T. Werfel (1996)
10.1016/S0091-6749(96)80007-8
Induction of IgE antibodies in mice and rhesus monkeys with recombinant birch pollen allergens: different allergenicity of Bet v 1 and Bet v 2.
S. Vrtala (1996)
10.1016/S0091-6749(96)80161-8
Biphasic response against aeroallergen in atopic dermatitis showing a switch from an initial TH2 response to a TH1 response in situ: an immunocytochemical study.
T. Thepen (1996)
10.1111/1523-1747.EP12329617
Immunoglobulin E response to human proteins in atopic patients.
R. Valenta (1996)
10.1046/J.1523-1747.1998.00413.X
Molecular characterization of an autoallergen, Hom s 1, identified by serum IgE from atopic dermatitis patients.
R. Valenta (1998)
10.1096/fasebj.12.14.1559
Isolation of cDNA clones coding for IgE autoantigens with serum IgE from atopic dermatitis patients
S. Natter (1998)
10.1084/JEM.189.9.1507
Humoral and Cell-mediated Autoimmune Reactions to Human Acidic Ribosomal P2 Protein in Individuals Sensitized to Aspergillus fumigatus P2 Protein
C. Mayer (1999)
10.1159/000024064
IgE–Mediated Reactions to Autoantigens in Allergic Diseases
U. Appenzeller (1999)
10.1159/000024229
Characterization of IgE–Reactive Autoantigens in Atopic Dermatitis
S. Seiberler (1999)
10.1096/fasebj.13.11.1277
B cell epitopes of the major timothy grass pollen allergen, Phl p 1, revealed by gene fragmentation as candidates for immunotherapy
Tanja Ball (1999)
10.1159/000024228
Characterization of IgE–Reactive Autoantigens in Atopic Dermatitis 1. Subcellular Distribution and Tissue–Specific Expression
S. Seiberler (1999)
10.1172/JCI5155
Allergen-specific Th1 cells fail to counterbalance Th2 cell-induced airway hyperreactivity but cause severe airway inflammation.
G. Hansen (1999)
10.1034/J.1399-3003.2000.01514.X
Tight junction properties of the immortalized human bronchial epithelial cell lines Calu-3 and 16HBE14o-.
H. Wan (2000)
10.1172/JCI9199
T cell-mediated Fas-induced keratinocyte apoptosis plays a key pathogenetic role in eczematous dermatitis.
A. Trautmann (2000)
10.1034/j.1600-0625.2001.100102.x
The eczema area and severity index (EASI): assessment of reliability in atopic dermatitis
J. Hanifin (2001)
10.1067/MAI.2001.118796
Targeting keratinocyte apoptosis in the treatment of atopic dermatitis and allergic contact dermatitis.
A. Trautmann (2001)
10.1146/ANNUREV.IMMUNOL.19.1.683
Interleukin-10 and the interleukin-10 receptor.
K. Moore (2001)
10.1067/MAI.2002.123303
IgE autoantibodies monitored in a patient with atopic dermatitis under cyclosporin A treatment reflect tissue damage.
T. Kinaciyan (2002)
10.1159/000066770
Carbohydrate Epitopes and Their Relevance for the Diagnosis and Treatment of Allergic Diseases
R. Ree (2002)
10.1046/J.1523-1747.2002.00518.X
Characterization of a novel isoform of alpha-nascent polypeptide-associated complex as IgE-defined autoantigen.
R. Mossabeb (2002)
10.4049/jimmunol.171.2.1078
A Second Step of Chemotaxis After Transendothelial Migration: Keratinocytes Undergoing Apoptosis Release IFN-γ-Inducible Protein 10, Monokine Induced by IFN-γ, and IFN-γ-Inducible α-Chemoattractant for T Cell Chemotaxis Toward Epidermis in Atopic Dermatitis1
S. Klunker (2003)
10.1111/j.1365-2249.2004.02649.x
Early up‐regulation of Th2 cytokines and late surge of Th1 cytokines in an atopic dermatitis model
L. Chen (2004)
10.1016/J.JACI.2004.05.029
Induction of autoallergy with an environmental allergen mimicking a self protein in a murine model of experimental allergic asthma.
R. Bünder (2004)
10.1046/j.1398-9995.2003.00330.x
IgE‐binding components of cultured human keratinocytes in atopic eczema/dermatitis syndrome and their crossreactivity with Malassezia furfur
O. Kortekangas-Savolainen (2004)
10.1016/J.JACI.2005.01.021
IFN-γ–enhanced allergen penetration across respiratory epithelium augments allergic inflammation
J. Reisinger (2005)
10.1111/j.1365-4632.2004.02234.x
Use of pimecrolimus cream 1% (Elidel®) in the treatment of atopic dermatitis in infants and children: the effects of ethnic origin and baseline disease severity on treatment outcome
L. Eichenfield (2005)
10.1016/J.JACI.2005.01.065
IgE-mediated and T cell-mediated autoimmunity against manganese superoxide dismutase in atopic dermatitis.
P. Schmid-Grendelmeier (2005)
10.1016/J.JACI.2005.06.025
The cradle of IgE autoreactivity in atopic eczema lies in early infancy.
N. Mothes (2005)
10.4049/jimmunol.175.2.1286
Hom s 4, an IgE-Reactive Autoantigen Belonging to a New Subfamily of Calcium-Binding Proteins, Can Induce Th Cell Type 1-Mediated Autoreactivity1
K. Aichberger (2005)
10.1111/j.1365-2133.2005.06508.x
Interferon (IFN)‐γ is a main mediator of keratinocyte (HaCaT) apoptosis and contributes to autocrine IFN‐γ and tumour necrosis factor‐α production
A. Konur (2005)
10.1016/J.JACI.2006.10.013
Exposure to a farming environment has allergen-specific protective effects on TH2-dependent isotype switching in response to common inhalants.
D. Stern (2007)
10.1038/nri2097
TH1 cells control themselves by producing interleukin-10
A. O’Garra (2007)



This paper is referenced by
10.1016/j.jaut.2021.102634
Autoreactive T cells and their role in atopic dermatitis.
Tina De Bruyn Carlier (2021)
10.1186/s13601-020-00338-7
IgE autoantibodies and autoreactive T cells and their role in children and adults with atopic dermatitis
F. Badloe (2020)
10.1016/j.jid.2019.07.703
Transglutaminase 3 promotes skin inflammation in atopic dermatitis by activating monocyte-derived dendritic cells via DC-SIGN.
Huichun Su (2019)
10.1007/s00403-019-01890-4
Atopic dermatitis and autoimmunity: the occurrence of autoantibodies and their association with disease severity
J. Holmes (2019)
10.3389/fimmu.2019.02128
Autoimmunity (or Not) in Atopic Dermatitis
L. Roesner (2019)
10.1016/j.intimp.2018.06.046
Regulatory effects of chrysophanol, a bioactive compound of AST2017‐01 in a mouse model of 2,4‐dinitrofluorobenzene‐induced atopic dermatitis
Na‐Ra Han (2018)
10.1111/all.13417
Molecular allergen profiling in horses by microarray reveals Fag e 2 from buckwheat as a frequent sensitizer
L. Einhorn (2018)
10.1007/978-3-319-69968-4_2
Microbial Triggers in Autoimmunity, Severe Allergy, and Autoallergy
F. M. S. Badloe (2017)
10.4049/jimmunol.1500351
α-NAC–Specific Autoreactive CD8+ T Cells in Atopic Dermatitis Are of an Effector Memory Type and Secrete IL-4 and IFN-γ
L. M. Roesner (2016)
10.1016/B978-0-323-29875-9.00050-1
50 – Role of Barrier Dysfunction and Immune Response in Atopic Dermatitis
N. Novak (2016)
10.1007/978-3-319-22243-1_3
Pathophysiology of Atopic Dermatitis/Eczema
J. Ring (2016)
10.1007/s00005-016-0400-3
Humoral and Cellular Autoreactivity to Epidermal Proteins in Atopic Dermatitis
C. Navarrete-Dechent (2016)
10.1586/1744666X.2016.1165093
The adaptive immune system in atopic dermatitis and implications on therapy
L. M. Roesner (2016)
10.1016/j.jaci.2014.10.038
Differential cytokine induction by the human skin-associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control subjects.
S. Hradetzky (2015)
10.1007/s40629-015-0037-5
Autoallergy in atopic dermatitis
S. Hradetzky (2015)
10.4155/CLI.14.108
Omalizumab for the treatment of atopic dermatitis
I. Eguiluz‐Gracia (2015)
10.1007/s15007-015-0737-7
Autoallergie bei atopischer Dermatitis
S. Hradetzky (2015)
10.1016/j.fsi.2015.06.033
Identification and expression analysis of nascent polypeptide-associated complex alpha gene in response to immune challenges in Japanese flounder Paralichthys olivaceus.
S. Li (2015)
10.1002/iub.1405
New insights into T cells and their signature cytokines in atopic dermatitis
Ao-xue Wang (2015)
10.1038/jid.2014.25
Cytokine effects induced by the human autoallergen α-NAC.
S. Hradetzky (2014)
10.1007/s00105-014-3559-8
Adaptive Immunantworten und damit verbundene Triggerfaktoren bei atopischer Dermatitis
A. Heratizadeh (2014)
10.4168/aair.2014.6.1.6
Histamine-Releasing Factor and Immunoglobulins in Asthma and Allergy
T. Kawakami (2014)
Sequential combined therapy with omalizumab and rituximab: a new approach to severe atopic dermatitis.
Silvia Sánchez-Ramón (2013)
10.1038/jid.2013.161
The human skin-associated autoantigen α-NAC activates monocytes and dendritic cells via TLR-2 and primes an IL-12-dependent Th1 response.
S. Hradetzky (2013)
Antigènes de blé et peptides pour le diagnostic de l'hypersensibilité induite par le blé
Alexandra Schmidhuber (2012)
10.4168/aair.2012.4.6.311
Highly Cytokinergic IgE Antibodies and Autoimmune Mechanisms
M. Kim (2012)
10.1007/978-1-62703-071-7_8
Exploring the diagnostic potential of immune biomarker coexpression in Gulf War Illness.
G. Broderick (2012)
10.4168/aair.2012.4.6.332
Most Highly Cytokinergic IgEs Have Polyreactivity to Autoantigens
J. Kashiwakura (2012)
10.1016/j.jaci.2012.02.002
Does "autoreactivity" play a role in atopic dermatitis?
T. Tang (2012)
10.1016/J.ADENGL.2012.08.002
Omalizumab for the treatment of atopic dermatitis.
M. C. Fernández-Antón Martínez (2012)
10.1097/ACI.0b013e32834a41dd
The IgE-microarray testing in atopic dermatitis: a suitable modern tool for the immunological and clinical phenotyping of the disease
A. Mari (2011)
10.21236/ada568442
The Use of Comprehensive Molecular Profiling With Network and Control Theory to Better Understand GWI and Model Therapeutic Strategies
N. Klimas (2011)
See more
Semantic Scholar Logo Some data provided by SemanticScholar