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The IgE-reactive Autoantigen Hom S 2 Induces Damage Of Respiratory Epithelial Cells And Keratinocytes Via Induction Of IFN-gamma.

I. Mittermann, R. Reininger, M. Zimmermann, K. Gangl, J. Reisinger, K. Aichberger, Elli K. Greisenegger, V. Niederberger, J. Seipelt, B. Bohle, T. Kopp, C. Akdis, S. Spitzauer, P. Valent, R. Valenta
Published 2008 · Medicine

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Hom s 2, the alpha-chain of the nascent polypeptide-associated complex, is an intracellular autoantigen that has been identified with IgE autoantibodies from atopic dermatitis patients. We investigated the humoral and cellular immune response to purified recombinant Hom s 2 (rHom s 2). rHom s 2 exhibited IgE reactivity comparable to exogenous allergens, but did not induce relevant basophil cell degranulation. The latter may be attributed to the fact that patients recognized single epitopes on Hom s 2 as revealed by IgE epitope mapping with rHom s 2 fragments. In contrast to exogenous allergens, rHom s 2 had the intrinsic ability to induce the release of IFN-gamma in cultured peripheral blood mononuclear cells from atopic as well as non-atopic individuals. IFN-gamma-containing culture supernatants from Hom s 2-stimulated peripheral blood mononuclear cells caused disintegration of respiratory epithelial cell layers and apoptosis of skin keratinocytes, which could be inhibited with a neutralizing anti-IFN-gamma antibody. Our data demonstrate that the Hom s 2 autoantigen can cause IFN-gamma-mediated cell damage.
This paper references
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
H. Towbin (1979)
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea.
S. Fling (1986)
Interleukin 3 activates human blood basophils via high-affinity binding sites.
P. Valent (1989)
Identification of profilin as a novel pollen allergen; IgE autoreactivity in sensitized individuals.
R. Valenta (1991)
Profilins constitute a novel family of functional plant pan-allergens
R. Valenta (1992)
Isolation of an immunodominant IgE hapten from an epitope expression cDNA library. Dissection of the allergic effector reaction.
T. Ball (1994)
CFTR expression and chloride secretion in polarized immortal human bronchial epithelial cells.
A. Cozens (1994)
Lesional expression of interferon-γ in atopic eczema
M. Grewe (1994)
Analysis of the cytokine pattern expressed in situ in inhalant allergen patch test reactions of atopic dermatitis patients.
M. Grewe (1995)
Humoral and cell-mediated autoimmunity in allergy to Aspergillus fumigatus
R. Crameri (1996)
Allergen specificity of skin-infiltrating T cells is not restricted to a type-2 cytokine pattern in chronic skin lesions of atopic dermatitis.
T. Werfel (1996)
Induction of IgE antibodies in mice and rhesus monkeys with recombinant birch pollen allergens: different allergenicity of Bet v 1 and Bet v 2.
S. Vrtala (1996)
Biphasic response against aeroallergen in atopic dermatitis showing a switch from an initial TH2 response to a TH1 response in situ: an immunocytochemical study.
T. Thepen (1996)
Immunoglobulin E response to human proteins in atopic patients.
R. Valenta (1996)
Molecular characterization of an autoallergen, Hom s 1, identified by serum IgE from atopic dermatitis patients.
R. Valenta (1998)
Isolation of cDNA clones coding for IgE autoantigens with serum IgE from atopic dermatitis patients
S. Natter (1998)
Humoral and Cell-mediated Autoimmune Reactions to Human Acidic Ribosomal P2 Protein in Individuals Sensitized to Aspergillus fumigatus P2 Protein
C. Mayer (1999)
IgE–Mediated Reactions to Autoantigens in Allergic Diseases
U. Appenzeller (1999)
Characterization of IgE–Reactive Autoantigens in Atopic Dermatitis
S. Seiberler (1999)
B cell epitopes of the major timothy grass pollen allergen, Phl p 1, revealed by gene fragmentation as candidates for immunotherapy
Tanja Ball (1999)
Characterization of IgE–Reactive Autoantigens in Atopic Dermatitis 1. Subcellular Distribution and Tissue–Specific Expression
S. Seiberler (1999)
Allergen-specific Th1 cells fail to counterbalance Th2 cell-induced airway hyperreactivity but cause severe airway inflammation.
G. Hansen (1999)
Tight junction properties of the immortalized human bronchial epithelial cell lines Calu-3 and 16HBE14o-.
H. Wan (2000)
T cell-mediated Fas-induced keratinocyte apoptosis plays a key pathogenetic role in eczematous dermatitis.
A. Trautmann (2000)
The eczema area and severity index (EASI): assessment of reliability in atopic dermatitis
J. Hanifin (2001)
Targeting keratinocyte apoptosis in the treatment of atopic dermatitis and allergic contact dermatitis.
A. Trautmann (2001)
Interleukin-10 and the interleukin-10 receptor.
K. Moore (2001)
IgE autoantibodies monitored in a patient with atopic dermatitis under cyclosporin A treatment reflect tissue damage.
T. Kinaciyan (2002)
Carbohydrate Epitopes and Their Relevance for the Diagnosis and Treatment of Allergic Diseases
R. Ree (2002)
Characterization of a novel isoform of alpha-nascent polypeptide-associated complex as IgE-defined autoantigen.
R. Mossabeb (2002)
A Second Step of Chemotaxis After Transendothelial Migration: Keratinocytes Undergoing Apoptosis Release IFN-γ-Inducible Protein 10, Monokine Induced by IFN-γ, and IFN-γ-Inducible α-Chemoattractant for T Cell Chemotaxis Toward Epidermis in Atopic Dermatitis1
S. Klunker (2003)
Early up‐regulation of Th2 cytokines and late surge of Th1 cytokines in an atopic dermatitis model
L. Chen (2004)
Induction of autoallergy with an environmental allergen mimicking a self protein in a murine model of experimental allergic asthma.
R. Bünder (2004)
IgE‐binding components of cultured human keratinocytes in atopic eczema/dermatitis syndrome and their crossreactivity with Malassezia furfur
O. Kortekangas-Savolainen (2004)
IFN-γ–enhanced allergen penetration across respiratory epithelium augments allergic inflammation
J. Reisinger (2005)
Use of pimecrolimus cream 1% (Elidel®) in the treatment of atopic dermatitis in infants and children: the effects of ethnic origin and baseline disease severity on treatment outcome
L. Eichenfield (2005)
IgE-mediated and T cell-mediated autoimmunity against manganese superoxide dismutase in atopic dermatitis.
P. Schmid-Grendelmeier (2005)
The cradle of IgE autoreactivity in atopic eczema lies in early infancy.
N. Mothes (2005)
Hom s 4, an IgE-Reactive Autoantigen Belonging to a New Subfamily of Calcium-Binding Proteins, Can Induce Th Cell Type 1-Mediated Autoreactivity1
K. Aichberger (2005)
Interferon (IFN)‐γ is a main mediator of keratinocyte (HaCaT) apoptosis and contributes to autocrine IFN‐γ and tumour necrosis factor‐α production
A. Konur (2005)
Exposure to a farming environment has allergen-specific protective effects on TH2-dependent isotype switching in response to common inhalants.
D. Stern (2007)
TH1 cells control themselves by producing interleukin-10
A. O’Garra (2007)

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Cytokine effects induced by the human autoallergen α-NAC.
S. Hradetzky (2014)
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S. Hradetzky (2013)
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J. Kashiwakura (2012)
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T. Tang (2012)
Omalizumab for the treatment of atopic dermatitis.
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The IgE-microarray testing in atopic dermatitis: a suitable modern tool for the immunological and clinical phenotyping of the disease
A. Mari (2011)
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