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Apoptosis In The Rat Mammary Gland And Ventral Prostate: Detection Of Cell Death-associated Genes Using A Coincident-expression Cloning Approach

W. Bielke, G. Ke, Z. Feng, S. Buhrer, S. Saurer, R. Friis
Published 1997 · Biology, Medicine

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Apoptosis plays a striking role in the hormone-dependent involution of the mammary gland, but it has proved difficult to distinguish between the ‘cell death’ associated genes and the ‘tissue remodelling’ genes which are expressed concurrently. To identify cell death-associated genes, we have established a ‘coincidence analysis’ based on the previously described ‘RNA differential display’ method of Liang and Pardee (1992). Coincidence analysis allows the detection of genes expressed during related processes in different organs and was employed here to identify transcripts in which expression patterns are seen to be associated with apoptosis during involution of both rat mammary- and the ventral prostate glands. That the coincidence analysis is a promising approach can be seen from the fact that while widely accepted apoptosis markers such as transglutaminase (Fesus et al, 1987; Strange et al, 1992) and sulfated glycoprotein-2 (Buttyan et al, 1989; Strange et al, 1992; Guenette et al 1994) exhibited similar expression in both regressing tissues, transcription of tissue remodelling enzymes was minimal in the involuting prostate. We describe here the characteristics of five clones isolated which show coincident expression during programmed cell death in mammary and prostate tissues. Partial sequence analysis revealed for three clones high homologies with previously described genes; the putative rat homolog of the growth arrest gene gas-1 (Schneider et al, 1988; Del Sal et al, 1992), an homolog of the mouse ‘Integrin Associated Protein’ (IAP) (Brown et al, 1990; Lindberg et al, 1993) and the sequence encoding for the ‘Allograft Inflammatory Factor’ AIF-1 (Autieri et al, 1995; Utans et al, 1995). One clone displayed homology with an expressed human sequence tag and one clone unrelated to any known DNA sequence was isolated. The expression of these genes in involuting rat mammary and ventral prostate, was correlated with that in other organs and in situ hybridization was applied to establish that the secretory epithelial cells which undergo programmed cell death are the site of elevated expression during the course of involution. Furthermore, we conclude that the coincidence analysis approach described here could be easily applied to facilitate the characterization of gene expression i.e. for the detection and comparison of hormonally regulated genes in different organs.
This paper references
Glucocorticoid activation of a calcium-dependent endonuclease in thymocyte nuclei leads to cell death.
J. Cohen (1984)
Differential display and cloning of messenger RNAs from human breast cancer versus mammary epithelial cells.
P. Liang (1992)
10.1083/JCB.118.5.1271
Coordinated expression of extracellular matrix-degrading proteinases and their inhibitors regulates mammary epithelial function during involution
R. Talhouk (1992)
10.1073/PNAS.89.16.7491
Wild-type p53 is a cell cycle checkpoint determinant following irradiation.
S. Kuerbitz (1992)
A 50-kDa integrin-associated protein is required for integrin-regulated calcium entry in endothelial cells.
M. Schwartz (1993)
10.1016/0968-0004(92)90325-4
Clusterin: the intriguing guises of a widely expressed glycoprotein.
D. Jenne (1992)
10.1128/MCB.14.3.1815
DNA strand breaks: the DNA template alterations that trigger p53-dependent DNA damage response pathways.
W. Nelson (1994)
10.1177/40.5.1315355
Enhanced synthesis of gelatinase and stromelysin by myoepithelial cells during involution of the rat mammary gland.
S. Dickson (1992)
10.1016/0092-8674(93)90486-A
Induction of apoptosis in fibroblasts by IL-1β-converting enzyme, a mammalian homolog of the C. elegans cell death gene ced-3
M. Miura (1993)
10.1126/SCIENCE.1354393
Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction.
P. Liang (1992)
10.7275/R5CJ8BPM
Cloning cell death genes.
L. M. Schwartz (1995)
10.1083/JCB.119.3.493
Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation
Y. Gavrieli (1992)
10.1038/284555A0
Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation
A. Wyllie (1980)
10.1128/MCB.15.12.7152
Gas1-induced growth suppression requires a transactivation-independent p53 function.
G. Del Sal (1995)
10.1016/s0021-9258(18)37999-7
The molecular basis of retinoic acid action. Transcriptional regulation of tissue transglutaminase gene expression in macrophages.
E. Chiocca (1988)
10.1096/fasebj.7.10.8344495
Identification by differential display of alpha 6 integrin as a candidate tumor suppressor gene
R. Sager (1993)
10.1016/0022-2836(79)90056-1
Changes in the abundance of polyadenylated RNA during slime mould development measured using cloned molecular hybridization probes.
J. Williams (1979)
10.1038/367277A0
Requirement for the orphan steroid receptor Nur77 in apoptosis of T-cell hybridomas
J. Woronicz (1994)
10.1677/JME.0.0120047
Induction of gene expression during involution of the lactating mammary gland of the rat.
R. Guenette (1994)
10.1002/j.1460-2075.1987.tb04804.x
Characterization and expression of a murine gene homologous to human EPA/TIMP: a virus‐induced gene in the mouse.
D. Gewert (1987)
10.1016/S0074-7696(08)62312-8
Cell death: the significance of apoptosis.
A. Wyllie (1980)
10.1016/S0092-8674(94)90422-7
Ich-1, an Ice/ced-3-related gene, encodes both positive and negative regulators of programmed cell death
Lin Wang (1994)
10.1016/0092-8674(93)90500-P
WAF1, a potential mediator of p53 tumor suppression
W. El-Deiry (1993)
10.1083/JCB.123.2.485
Molecular cloning of integrin-associated protein: an immunoglobulin family member with multiple membrane-spanning domains implicated in alpha v beta 3-dependent ligand binding
F. P. Lindberg (1993)
10.1016/0092-8674(79)90020-5
Isolation of galactose-inducible DNA sequences from Saccharomyces cerevisiae by differential plaque filter hybridization
T. P. John (1979)
10.1007/978-1-4899-5043-7_1
Postnatal Development of the Rodent Mammary Gland
C. Daniel (1987)
The tumor suppressor p 53 . Biochim . Biophys
LA Donehower (1993)
10.1016/S0888-7543(05)80388-X
Localization of the human growth arrest-specific gene (GAS1) to chromosome bands 9q21.3-q22, a region frequently deleted in myeloid malignancies.
A. Evdokiou (1993)
10.1007/978-1-349-06859-3_26
The Mammary Gland
D. Pitelka (1983)
Apoptosis. The role of the endonuclease.
M. Arends (1990)
10.1038/367281A0
Apoptotic signals delivered through the T-cell receptor of a T-cell hybrid require the immediate–early gene nur77
Z. Liu (1994)
death gene ced - 3
T Miyashita (1995)
The tumor suppressor p53.
L. Donehower (1993)
10.1016/0092-8674(93)90485-9
The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1β-converting enzyme
J. Yuan (1993)
10.1002/PROS.2990130203
Activation of a Ca2+−Mg2+‐dependent endonuclease as an early event in castration‐induced prostatic cell death
N. Kyprianou (1988)
10.1016/S0022-5347(17)39975-5
Characterization of the products of a gene expressed during androgen-programmed cell death and their potential use as a marker of urogenital injury.
M. Bandyk (1990)
10.1007/BF02889300
Deletion of cells by apoptosis during castration-induced involution of the rat prostate
J. Kerr (1973)
10.1101/GAD.8.14.1613
Induction of apoptosis by the mouse Nedd2 gene, which encodes a protein similar to the product of the Caenorhabditis elegans cell death gene ced-3 and the mammalian IL-1 beta-converting enzyme.
S. Kumar (1994)
10.1096/fasebj.3.7.2497041
Calcium‐activated DNA fragmentation kills immature thymocytes
D. McConkey (1989)
10.1002/j.1460-2075.1993.tb05666.x
Characterization of the endogenous deoxyribonuclease involved in nuclear DNA degradation during apoptosis (programmed cell death).
M. Peitsch (1993)
10.1615/CRITREVEUKARGENEEXPR.V7.I1-2.100
Gene regulation associated with apoptosis.
B. M. Jehn (1997)
Integrin b 3 cytoplasmic tail is necessary and sufficient for regulation of the a 5 b 1 phagocytosis by a v b 3 and integrin - associated protein
SD Blystone (1995)
10.1038/316701A0
Human transforming growth factor-β complementary DNA sequence and expression in normal and transformed cells
R. Derynck (1985)
10.1083/JCB.111.6.2785
Integrin-associated protein: a 50-kD plasma membrane antigen physically and functionally associated with integrins
E. Brown (1990)
10.1016/0014-5793(87)80430-1
Induction and activation of tissue transglutaminase during programmed cell death
L. Fesus (1987)
10.1016/0092-8674(92)90429-G
The growth arrest-specific gene, gas1, is involved in growth suppression
G. Sal (1992)
10.1093/NAR/21.18.4272
Identification of differentially expressed mRNA species by an improved display technique (DDRT-PCR).
D. Bauer (1993)
10.1007/978-1-4615-1973-7_5
Apoptosis in Mammary Gland Involution: Isolation and Characterization of Apoptosis-Specific Genes
W. Bielke (1995)
10.1038/308149A0
Isolation of cDNA clones encoding T cell-specific membrane-associated proteins
S. Hedrick (1984)
10.1038/bjc.1972.33
Apoptosis: A Basic Biological Phenomenon with Wide-ranging Implications in Tissue Kinetics
J. Kerr (1972)
10.1073/PNAS.88.8.3412
Coordinated regulation of apoptosis and cell proliferation by transforming growth factor beta 1 in cultured uterine epithelial cells.
R. Rotello (1991)
10.1073/PNAS.89.10.4495
Induction of apoptosis by wild-type p53 in a human colon tumor-derived cell line.
P. Shaw (1992)
10.1016/0092-8674(91)90614-5
The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis
N. Itoh (1991)
10.1016/S0092-8674(88)91065-3
Genes specifically expressed at growth arrest of mammalian cells
C. Schneider (1988)
10.1126/SCIENCE.2787530
Monoclonal antibody-mediated tumor regression by induction of apoptosis.
B. Trauth (1989)
10.1002/j.1460-2075.1985.tb03799.x
Epidermal growth factor and oncogenes induce transcription of the same cellular mRNA in rat fibroblasts.
L. Matrisian (1985)
Apoptotic cell death and tissue remodelling during mouse mammary gland involution.
R. Strange (1992)
Use of differential display to identify differentially expressed mRNAs induced by rat carotid artery balloon angioplasty.
M. Autieri (1995)
10.1073/PNAS.89.19.9210
Growth arrest induced by wild-type p53 protein blocks cells prior to or near the restriction point in late G1 phase.
D. Lin (1992)
Protein kinase A and AP-1 (c-Fos/JunD) are induced during apoptosis of mouse mammary epithelial cells.
A. Martí (1994)
Isolation of cell death-associated cDNAs from involuting mouse mammary epithelium.
F. Li (1995)
10.1016/0092-8674(92)90593-2
A mammalian cell cycle checkpoint pathway utilizing p53 and GADD45 is defective in ataxia-telangiectasia
M. Kastan (1992)
10.1073/PNAS.92.9.3978
Transendothelial migration of neutrophils involves integrin-associated protein (CD47).
D. Cooper (1995)
10.1210/ENDO-127-6-2963
Effect of transforming growth factor-beta 1 on proliferation and death of rat prostatic cells.
P. Martikainen (1990)
10.1016/0022-1910(64)90034-4
Programmed cell death—II. Endocrine potentiation of the breakdown of the intersegmental muscles of silkmoths
R. Lockshin (1964)
Induction of apoptosis in fibroblasts by IL - 1 beta - converting enzyme , a mammalian homolog of the C . elegans
M Miura (1993)
10.1128/MCB.9.8.3473
Induction of the TRPM-2 gene in cells undergoing programmed death.
R. Buttyan (1989)
10.1128/MCB.13.1.711
Cell cycle analysis of p53-induced cell death in murine erythroleukemia cells.
J. J. Ryan (1993)
10.1016/0092-8674(93)90326-L
Molecular cloning and expression of the fas ligand, a novel member of the tumor necrosis factor family
T. Suda (1993)
10.1172/JCI118003
Cloning and characterization of allograft inflammatory factor-1: a novel macrophage factor identified in rat cardiac allografts with chronic rejection.
U. Utans (1995)
10.1073/PNAS.91.14.6463
Chronic cardiac rejection: identification of five upregulated genes in transplanted hearts by differential mRNA display.
U. Utans (1994)
10.1002/PROS.2990130204
Calcium channel antagonists delay regression of androgen‐dependent tissues and suppress gene activity associated with cell death
J. Connor (1988)
10.1016/0092-8674(95)90412-3
Tumor suppressor p53 is a direct transcriptional activator of the human bax gene
Miyashita Toshiyuki (1995)
10.1177/30.7.6179984
Distribution of myoepithelial cells and basement membrane proteins in the resting, pregnant, lactating, and involuting rat mammary gland.
M. Warburton (1982)
10.1002/AJA.1001850104
Cell death by apoptosis during involution of the lactating breast in mice and rats.
N. Walker (1989)
10.1038/352345A0
Wild-type p53 induces apoptosis of myeloid leukaemic cells that is inhibited by interleukin-6
E. Yonish-Rouach (1991)
10.1016/0003-2697(87)90021-2
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
P. Chomczyński (1987)
10.1016/0092-8674(80)90434-1
Molecular cloning and selection of genes regulated in aspergillus development
C. Zimmermann (1980)
10.1016/0092-8674(93)90499-G
The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases
J. Harper (1993)
Hormone-regulated apoptosis results from reentry of differentiated prostate cells onto a defective cell cycle.
M. Colombel (1992)
Programmed Cell Death-II
RA Lockshin (1964)
Two distinct phases of apoptosis in mammary gland involution: proteinase-independent and -dependent pathways.
L. Lund (1996)



This paper is referenced by
10.1210/endo.140.10.7097
A Novel Gene Overexpressed in the Prostate of Castrated Rats: Hormonal Regulation, Relationship to Apoptosis and to Acquired Prostatic Cell Androgen Independence1.
M. Bruyninx (1999)
10.1038/sj.cdd.4401197
Role of DDC-4/sFRP-4, a secreted Frizzled-related protein, at the onset of apoptosis in mammary involution
M. Lacher (2003)
10.1016/S0014-5793(97)01324-0
DDC‐4, an apoptosis‐associated gene, is a secreted frizzled relative
V. Wolf (1997)
Novel three gene molecular signature for prostate cancer recurrence
Raymond B. Nagle (2015)
10.1186/1750-2187-3-10
SFRP-4 abrogates Wnt-3a-induced β-catenin and Akt/PKB signalling and reverses a Wnt-3a-imposed inhibition of in vitro mammary differentiation
Thecla Constantinou (2008)
10.1074/jbc.M509572200
Gas1 Is Related to the Glial Cell-derived Neurotrophic Factor Family Receptors α and Regulates Ret Signaling*
J. R. Cabrera (2006)
10.1016/S1472-6483(10)60431-2
Expression of secreted frizzled-related protein 4 in the primate placenta.
L. White (2009)
10.1038/sj.cdd.4400610
Physiological apoptosis in hormone-dependent tissues: involvement of caspases
A. Marti (1999)
10.1016/S0037-1963(01)90007-1
Exploring (novel) gene expression during retinoid-induced maturation and cell death of acute promyelocytic leukemia.
G. Benoit (2001)
10.1096/fasebj.12.14.1541
The epithelium‐specific Ets transcription factor ESX is associated with mammary gland development and involution
R. Neve (1998)
10.1023/A:1011384009787
Clearance: The Last and Often Forgotten Stage of Apoptosis
V. Fadok (2004)
10.1023/A:1024181203729
The role of sFRP4, a secreted frizzled-related protein, in ovulation
J. Drake (2004)
10.1016/j.mce.2006.02.013
Lactoferrin (Lf): Retinoid interactions in the mammary glands of transgenic mice overexpressing human Lf
C. Baumrucker (2006)
10.1006/mcne.2001.1092
Gas1 Is Induced during and Participates in Excitotoxic Neuronal Death
B. Mellström (2002)
10.1095/BIOLREPROD58.3.739
Apoptosis-associated gene expression in the corpus luteum of the rat.
K. Guo (1998)
10.1007/s13277-014-2956-z
Secreted frizzled-related protein 4 and its implications in cancer and apoptosis
S. Pohl (2014)
10.2174/157436211794109433
Molecular Mechanisms of Action of Gas1 and its Possible Therapeutic Applications
G. Domínguez-Monzón (2011)
10.1002/jnr.10461
Transcriptionally mediated gene targeting of gas1 to glioma cells elicits growth arrest and apoptosis
Absalom Zamorano (2003)
10.1016/j.nbd.2003.11.022
Glial-specific retrovirally mediated gas1 gene expression induces glioma cell apoptosis and inhibits tumor growth in vivo
Absalom Zamorano (2004)
10.1071/RD15367
Growth arrest specific 1 (Gas1) and glial cell line-derived neurotrophic factor receptor ?1 (Gfr?1), two mouse oocyte glycosylphosphatidylinositol-anchored proteins, are involved in fertilisation.
M. Agopiantz (2016)
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