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Liver-targeted Gene Transfer Into A Human Hepatoblastoma Cell Line And In Vivo By Sterylglucoside-containing Cationic Liposomes
S. Hwang, K. Hayashi, K. Takayama, Y. Maitani
Published 2001 · Biology, Medicine
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We investigated the transfection efficiency of β-sitosterol β-D-glucoside (Sit-G)-containing liposome/DNA complex (Sit-G-liposome/DNA complex) for liver targeting. The Sit-G-liposome/DNA complex was composed of Tfx-20 reagent (Tfx), ie synthetic cationic lipid [N,N,N′,N′-tetramethyl-N,N′-bis(2-hydroxyethyl)-2,3-di(oleoyloxy)-1,4-butanediammonium iodide] with L-dioleoylphosphatidylethanolamine (DOPE), 3β[N-(N′,N′-dimethylaminoethane)-carbamoyl]cholesterol (DC-Chol) and Sit-G with plasmid DNA. The in vitro studies were performed in HepG2 cells in serum-containing medium and the in vivo studies were carried out in the mice following intravenous injection. The Sit-G-liposome produced a Sit-G-liposome/DNA complex of relatively small size (100–250 nm). Transfection efficiency of the luciferase marker gene by Sit-G-liposome/DNA complex was increased in the presence of 10% serum in vitro, and was selectively high in the mouse liver reaching expression values up to an average of 14.9 pg luciferase/mg tissue protein, compared with Tfx/DNA complex, which showed approximately three-fold higher gene expression than Sit-G-liposome/DNA complex in vitro. High in vitro transfection efficiency by Sit-G-liposome/DNA complex seemed to be possible even with large lipid precipitates, whereas high in vivo activity seemed to be related to small and dispersed complexes. The interaction of liposome/DNA complexes with serum may be a key point to predict the in vivo efficiency of a liposome vector.
This paper references
Human serum albumin enhances DNA transfection by lipoplexes and confers resistance to inhibition by serum.
S. Simões (2000)
Receptor-mediated gene delivery and expression in vivo.
G. Wu (1988)
In vivo gene transfer via intravenous administration of cationic lipid–protamine–DNA (LPD) complexes
S. Li (1997)
Efficiency of liposomes surface-modified with soybean-derived sterylglucoside as a liver targeting carrier in HepG2 cells.
Y. Maitani (2001)
Receptor-mediated transfer of pSV2CAT DNA to a human hepatoblastoma cell line HepG2 using asialofetuin-labeled cationic liposomes.
T. Hara (1995)
Evaluation of dipalmitoylphosphatidylcholine liposomes containing a soybean-derived sterylglucoside mixture for liver targeting.
K. Shimizu (1996)
Pharmacokinetics, tissue distribution, and expression efficiency of plasmid [33P]DNA following intravenous administration of DNA/cationic lipid complexes in mice: use of a novel radionuclide approach.
G. Osaka (1996)
A novel cationic liposome reagent for efficient transfection of mammalian cells.
X. Gao (1991)
Formulation of liposomes with a soybean-derived sterylglucoside mixture and cholesterol for liver targeting.
K. Shimizu (1997)
Targeting of soybean-derived sterylglucoside liposomes to liver tumors in rat and mouse models.
K. Shimizu (1998)
Characterization of cationic liposome-mediated gene transfer in vivo by intravenous administration.
Y. Song (1997)
Gene therapy for cystic fibrosis: a clinical perspective.
E. Alton (1995)
Effect of serum components on the physico-chemical properties of cationic lipid/oligonucleotide complexes and on their interactions with cells.
O. Zelphati (1998)
Improved DNA: liposome complexes for increased systemic delivery and gene expression
Nancy Smyth Templeton (1997)
Effects of fusogenic and DNA-binding amphiphilic compounds on the receptor-mediated gene transfer into hepatic cells by asialofetuin-labeled liposomes.
T. Hara (1996)
Lactose-poly(ethylene glycol)-grafted poly-L-lysine as hepatoma cell-tapgeted gene carrier.
Y. Choi (1998)
Targeted gene transfer into hepatoma cells with lipopolyamine-condensed DNA particles presenting galactose ligands: a stage toward artificial viruses.
J. S. Rémy (1995)
Non-viral gene therapy.
D. Treco (1995)
Physicochemical characteristics and transfection efficiency of DNA in cationic and neutral liposomes with soybean-derived sterylglucoside into HepG2 cells
Y Maitani (2001)
MODIFIED ETHANOL INJECTION METHOD FOR LIPOSOMES CONTAINING β-SITOSTEROL β-D-GLUCOSIDE
Y. Maitani (2001)
Reproducible and efficient murine CNS gene delivery using a microprocessor-controlled injector
A. Brooks (1998)
Direct gene transfer with DNA-liposome complexes in melanoma: expression, biologic activity, and lack of toxicity in humans.
G. Nabel (1993)
Increased liver uptake of liposomes and improved targeting efficacy by labeling with asialofetuin in rodents
J. Wu (1998)
Physicochemical Characteristics and Transfection Efficiency of DNA in Liposomes with Soybean-Derived Sterylglucoside into HepG2 Cells
Y. Maitani (2001)
Genistein inhibition of fast Na+ current in uterine leiomyosarcoma cells is independent of tyrosine kinase inhibition.
M. Kusaka (1996)
New structures in complex formation between DNA and cationic liposomes visualized by freeze—fracture electron microscopy
B. Sternberg (1994)
Stabilization of cationic liposome‐plasmid DNA complexes by polyamines and poly(ethylene glycol)‐phospholipid conjugates for efficient in vivo gene delivery
K. Hong (1997)
Systemic gene expression after intravenous DNA delivery into adult mice.
N. Zhu (1993)
This paper is referenced by
CpG-depleted plasmid DNA vectors with enhanced safety and long-term gene expression in vivo.
N. Yew (2002)
Intracellular trafficking is the important process that determines the optimal charge ratio on transfection by galactosylated lipoplex in HEPG2 cells.
Yasunori Saito (2006)
Folate-targeted, cationic liposome-mediated gene transfer into disseminated peritoneal tumors
J. Reddy (2002)
Nano-vectors for efficient liver specific gene transfer
A. Pathak (2008)
Biochemical and biophysical characteristics of lipoplexes pertinent to solid tumour gene therapy.
C. Dass (2002)
Mechanisms of co-modified liver-targeting liposomes as gene delivery carriers based on cellular uptake and antigens inhibition effect.
Y. Zhang (2007)
Vector System: Plasmid DNA
Rajkumar Banerjee (2006)
Carbohydrate mediated drug delivery: synthesis and characterization of new lipid-conjugates.
M. Ahmad (2015)
Cationic glycolipids with cyclic and open galactose head groups for the selective targeting of genes to mouse liver.
Rajesh Mukthavaram (2009)
Development of a single cell electroporation method using a scanning ion conductance microscope with a theta type probe pipette
S. Sakurai (2014)
Novel Targeted Liposomes Deliver siRNA to Hepatocellular Carcinoma Cells in vitro
Shantal Dorasamy (2012)
A Novel Cationic Cholesterol Derivative, Its Formulation into Liposomes, and the Efficient Transfection of the Transformed Human Cell Lines HepG2 and HeLa
N. Kisoon (2002)
Novel cationic lipoplexes : characterization in cell culture in vitro and in vivo.
Alisha. Sewbalas (2010)
Plasmid DNA–Mediated Gene Therapy
Rajkumar Banerjee (2003)
Formulaciones biofarmacéuticas de ácidos desoxiribonucléicos en liposomas
E. Pupo ()
Poly(cationic lipid)-mediated in vivo gene delivery to mouse liver
L. Liu (2003)
Effect of the particle size of galactosylated lipoplex on hepatocyte-selective gene transfection after intraportal administration.
Y. Higuchi (2006)
Drug delivery systems based on sugar-macromolecule conjugates.
B. Davis (2002)
The Role of Organ Vascularization and Lipoplex-Serum Initial Contact in Intravenous Murine Lipofection*
D. Simberg (2003)
Local electroporation of a single cell using a scanning ion conductance microscope
F. Iwata (2014)
Silymarin loaded liposomes for hepatic targeting: in vitro evaluation and HepG2 drug uptake.
M. Elmowafy (2013)
Development of a single cell electroporation method using a scanning ion conductance microscope with a theta nanopipette
S. Sakurai (2015)
A molecular delivery system by using AFM and nanoneedle.
S. W. Han (2005)
Liposome Vector Containing Biosurfactant-Complexed DNA as Herpes Simplex Virus Thymidine Kinase Gene Delivery System
Y. Maitani (2006)
Synthetic Vectors for Genetic Drug Delivery
P. Wyrozumska (2006)
Cytotoxicity issues pertinent to lipoplex‐mediated gene therapy in‐vivo
C. Dass (2002)
Gene Therapy with Plasmid DNA
S. R. Bathula (2010)
Double Liposomes: Hypoglycemic Effects of Liposomal Insulin on Normal Rats
Ken Katayama (2003)
Targeted cationic liposomes, technologies, and developments
Anas El-Aneed (2003)
Carbohydrate-based materials for targeted delivery of drugs and genes to the liver.
M. Ahmed (2015)
Transfection efficiency of pORF lacZ plasmid lipopolyplex to hepatocytes and hepatoma cells.
Xun Sun (2004)
Liposomes: Design and Manufacturing
S. Patil (2005)See more