Please confirm you are human (Sign Up for free to never see this)
← Back to Search
Synthesis And Characterization Of PH-sensitive Poly(N-2-hydroxyethyl Acrylamide)–acrylic Acid (poly(HEAA/AA)) Nanogels With Antifouling Protection For Controlled Release
C. Zhao, Qiang Chen, Kunal Patel, L. Li, Xiaosi Li, Q. Wang, G. Zhang, J. Zheng
Published 2012 · Chemistry
Save to my Library
Download PDFAnalyze on Scholarcy
Major challenges in the development of effective stimuli-responsive nanogels as targeted drug and gene delivery carriers are to improve biocompatibility, stability, and controlled release during systemic circulation. In this work, biofunctional nanogels based on copolymers of poly(N-2-hydroxyethyl acrylamide) (polyHEAA) and acrylic acid (AA) with controlled size and morphology are synthesized using an inverse-microemulsion free-radical polymerization method. The chemical composition, size, swelling behavior, physical stability, and antifouling ability of the poly(HEAA/AA) nanogels are characterized by FTIR, dynamic light scattering, and atomic force microscopy. The cell viability and uptake of the nanogels are evaluated under physiological conditions. The controlled release of drugs in the nanogels is measured in both aqueous media and cell culture upon changes in pH and salt concentration. The poly(HEAA/AA) nanogels exhibit both superlow fouling ability to resist nonspecific protein adsorption and ultrastability to keep their hydrodynamic sizes unchanged in 100% human blood plasma for 30 days in vitro. The controlled release of Rhodamine 6G encapsulated in poly(HEAA/AA) nanogels is demonstrated at a low pH and high salt concentration. More importantly, the conjugation of transferrin with poly(HEAA/AA) nanogels allows the uptake of nanogels by SH-SY5Y cells and triggering of intracellular drug release, while not inducing cytotoxicity to cells similar to innate cell viability. HEAA/AA-based nanogels hold great potential as drug delivery carriers in vivo, because they integrate ultrastability, superlow fouling ability, and environmental-responsive controlled release property into one chemical entity.
This paper references
Film thickness dependence of protein adsorption from blood serum and plasma onto poly(sulfobetaine)-grafted surfaces.
W. Yang (2008)
Multivariate surface analysis of plasma-deposited tetraglyme for reduction of protein adsorption and monocyte adhesion
M. Shen (2003)
Poly(ethylene glycol)-based thiol-ene hydrogel coatings-curing chemistry, aqueous stability, and potential marine antifouling applications.
P. Lundberg (2010)
Functionalizable and ultrastable zwitterionic nanogels.
G. Cheng (2010)
Self-assembled monolayers of alkanethiolates presenting mannitol groups are inert to protein adsorption and cell attachment
Y. Luk (2000)
Long-term stability of grafted polyethylene glycol surfaces for use with microstamped substrates in neuronal cell culture.
D. Branch (2001)
Advanced nanogel engineering for drug delivery
K. Raemdonck (2009)
Stability and effectiveness against bacterial adhesion of poly(ethylene oxide) coatings in biological fluids.
A. Roosjen (2005)
Stimuli-reponsive polymers and their bioconjugates
E. S. Gil (2004)
Polymeric vesicles: from drug carriers to nanoreactors and artificial organelles.
P. Tanner (2011)
Effect of film thickness on the antifouling performance of poly(hydroxy-functional methacrylates) grafted surfaces.
C. Zhao (2011)
Design and fabrication of magnetic nanoparticles for targeted drug delivery and imaging.
O. Veiseh (2010)
Polymeric nanogels produced via inverse microemulsion polymerization as potential gene and antisense delivery agents.
K. McAllister (2002)
Dual-functional alginic acid hybrid nanospheres for cell imaging and drug delivery.
R. Guo (2009)
Zwitterionic carboxybetaine polymer surfaces and their resistance to long-term biofilm formation.
G. Cheng (2009)
Poly(N-vinylformamide) Nanogels Capable of pH-Sensitive Protein Release
L. Shi (2008)
Synthesis and characterization of glycerol dendrons, self-assembled monolayers on gold: a detailed study of their protein resistance.
M. Wyszogrodzka (2009)
Nonfouling characteristics of dextran-containing surfaces.
Surangkhana Martwiset (2006)
Pursuing "zero" protein adsorption of poly(carboxybetaine) from undiluted blood serum and plasma.
W. Yang (2009)
Hydrogen-bonded multilayer of pH-responsive polymeric micelles with tannic acid for surface drug delivery.
Byeong-Su Kim (2009)
Protein adsorption and cellular uptake of cerium oxide nanoparticles as a function of zeta potential.
Swanand D. Patil (2007)
Comparative drug release studies of two cationic drugs from pH-responsive nanogels.
Jeremy P. K. Tan (2007)
Synthesis and characterization of poly(N-hydroxyethylacrylamide) for long-term antifouling ability.
C. Zhao (2011)
Biomimetic engineering of non-adhesive glycocalyx-like surfaces using oligosaccharide surfactant polymers
N. B. Holland (1998)
Poly-2-methyl-2-oxazoline: a peptide-like polymer for protein-repellent surfaces.
Rupert Konradi (2008)
Renal clearance of quantum dots
H. Choi (2007)
Acidic pH-responsive nanogels as smart cargo systems for the simultaneous loading and release of short oligonucleotides and magnetic nanoparticles.
S. R. Deka (2010)
Nanostructured hybrid hydrogels prepared by a combination of atom transfer radical polymerization and free radical polymerization.
S. Bencherif (2009)
Extracellular pH distribution in human tumours.
K. Engin (1995)
Dual stimuli responsive hollow nanogels with IPN structure for temperature controlling drug loading and pH triggering drug release
Z. Xing (2011)
Biofunctionalized pH‐Responsive Microgels for Cancer Cell Targeting: Rational Design
M. Das (2006)
Micro/nanostructured polymeric systems for biomedical and pharmaceutical applications.
F. Chiellini (2008)
Strong Resistance of Poly (Ethylene Glycol) Based L-tyrosine Polyurethanes to Protein Adsorption and Cell Adhesion
Jui-Chen Yang (2012)
Functional polymer thin films designed for antifouling materials and biosensors
C. Zhao (2012)
Achieving highly effective nonfouling performance for surface-grafted poly(HPMA) via atom-transfer radical polymerization.
C. Zhao (2010)
Nanogels as pharmaceutical carriers: finite networks of infinite capabilities.
A. Kabanov (2009)
pH-Sensitive Nanogel Possessing Reactive PEG Tethered Chains on the Surface
H. Hayashi (2004)
Surface Hydration: Principles and Applications Toward Low-fouling/nonfouling Biomaterials
Shenfu Chen (2010)
Synthesis and characterization of novel dual-responsive nanogels and their application as drug delivery systems.
Jin-rong Peng (2012)
Stimuli-responsive smart nanogels for cancer diagnostics and therapy.
Motoi Oishi (2010)
A pH-sensitive nano drug delivery system derived from pullulan/doxorubicin conjugate.
D. Lu (2009)
Antifouling ability of polyethylene glycol of different molecular weights grafted onto polyester surfaces by cold plasma
Baiyan Dong (2011)
Tetraglyme coatings reduce fibrinogen and von Willebrand factor adsorption and platelet adhesion under both static and flow conditions.
Min Zhang (2009)
Recent progress in tumor pH targeting nanotechnology.
E. Lee (2008)
Tunable bioadhesive copolymer hydrogels of thermoresponsive poly(N-isopropyl acrylamide) containing zwitterionic polysulfobetaine.
Yung Chang (2010)
Stimulus responsive nanogels for drug delivery
Liusheng Zha (2011)
Intracellular uptake of anionic superparamagnetic nanoparticles as a function of their surface coating.
C. Wilhelm (2003)
This paper is referenced by
Nanogels as nanocarriers for drug delivery: A review
R. Rajput (2020)
Développement de chimie de surface pour la réduction de l’adsorption non-spécifique de lysat cellulaire et application clinique de biocapteurs SPR
Alexandra Aubé (2017)
C/W emulsion‐templated macroporous anionic monolith: Application for dye removal
Yunxia Zhang (2020)
Highly efficient antibacterial surface grafted with a triclosan-decorated poly(N-hydroxyethylacrylamide) brush.
Haixia Wu (2015)
Novel Aqueous Microgels and Their Applications
S. Tehrani (2017)
Sulfated zwitterionic poly(sulfobetaine methacrylate) hydrogels promote complete skin regeneration.
Jiang Wu (2018)
Self-Assembly of Soft Cellulose Nanospheres into Colloidal Gel Layers with Enhanced Protein Adsorption Capability for Next-Generation Immunoassays.
Katariina Solin (2020)
Preparation and characterization of pH responsive poly(methacrylic acid-acrylamide-N-hydroxyethyl acrylamide) hydrogels for drug delivery systems
Ayça Bal (2016)
Fabrication of thermo-responsive cotton fabrics using poly(vinyl caprolactam-co-hydroxyethyl acrylamide) copolymer.
M. Xiao (2017)
A comparative study of the mechanical properties of hybrid double-network hydrogels in swollen and as-prepared states.
H. Chen (2016)
Rational design of dendritic thermoresponsive nanogels that undergo phase transition under endolysosomal conditions.
G. N. Rimondino (2017)
Preparation and characterization of pH-sensitive and antifouling poly(vinylidene fluoride) microfiltration membranes blended with poly(methyl methacrylate-2-hydroxyethyl methacrylate-acrylic acid).
J. Ju (2014)
Biofouling-resistance control of expanded poly(tetrafluoroethylene) membrane via atmospheric plasma-induced surface PEGylation
A. Venault (2013)
Frontiers in the design and synthesis of advanced nanogels for nanomedicine
G. Liu (2014)
Poly(methylmethacrylate)-block-poly(N-hydroxyethylacrylamide) diblock copolymers: direct ATRP synthesis and characterization
M. Ashaduzzaman (2013)
Graphene/cyclodextrin-based nanocomposite hydrogel with enhanced strength and thermo-responsive ability.
P. Zhu (2017)
Synthesis of polymeric nano/microgels: a review
Y. Hamzah (2017)
ATRP of N-Hydroxyethyl Acrylamide in the Presence of Lewis Acids: Control of Tacticity, Molecular Weight, and Architecture.
Yue Sun (2019)
Super Bulk and Interfacial Toughness of Physically Crosslinked Double-Network Hydrogels
H. Chen (2017)
Controlled release profile of 5-fluorouracil loaded P(AAM-co-NVP-co-DEAEMA) microgel prepared via free radical precipitation polymerization
Zehra Özbaş (2017)
Non-equilibrium polymerization enables adhesive material with anti-freezing, multipurpose adhesion, long-term air stability and anisotropic deformation
Kaixiang Yang (2020)
Nanoclay Reinforced Self‐Cross‐Linking Poly(N‐Hydroxyethyl Acrylamide) Hydrogels with Integrated High Performances
Xianqiang Yu (2018)
Phosphorylcholine-based encoded hydrogel microparticles with enhanced fouling resistance for multiplex immunoassays.
Y. H. Roh (2020)
Synthesis of Brush-Like Glycopolymers with Monodisperse, Sequence-Defined Side Chains and Their Interactions with Plant and Animal Lectins.
F. Shamout (2019)
Stimuli-Responsive Microgels and Microgel-Based Systems: Advances in the Exploitation of Microgel Colloidal Properties and Their Interfacial Activity
G. Agrawal (2018)
Influence of Block versus Random Monomer Distribution on the Cellular Uptake of Hydrophilic Copolymers
J. Moraes (2016)
Antifouling performance of nano-sized spherical poly(N-hydroxyethyl acrylamide) brush.
Xue Qin (2017)
Hydrogel Nanoparticles with Thermally Controlled Drug Release
T. Shirakura (2014)
Boronate-functionalized hydrogel as a novel biosensing interface for the glycated hemoglobin A1c (HbA1c) based on the competitive binding with signaling glycoprotein.
Y. Han (2017)
Molecular Understanding and Structural-Based Design of Polyacrylamides and Polyacrylates as Antifouling Materials.
Hong Chen (2016)
Core–shell nanogel of PEG–poly(aspartic acid) and its pH-responsive release of rh-insulin
C. Park (2013)
Integration of antifouling and antibacterial properties in salt-responsive hydrogels with surface regeneration capacity.
D. Zhang (2018)See more