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Microfluidic Technologies For Circulating Tumor Cell Isolation.
Hyungseok Cho, Jinho Kim, H. Song, K. Y. Sohn, Minhyon Jeon, K. Han
Published 2018 · Medicine
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Metastasis is the main cause of tumor-related death, and the dispersal of tumor cells through the circulatory system is a critical step in the metastatic process. Early detection and analysis of circulating tumor cells (CTCs) is therefore important for early diagnosis, prognosis, and effective treatment of cancer, enabling favorable clinical outcomes in cancer patients. Accurate and reliable methods for isolating and detecting CTCs are necessary to obtain this clinical information. Over the past two decades, microfluidic technologies have demonstrated great potential for isolating and detecting CTCs from blood. The present paper reviews current advanced microfluidic technologies for isolating CTCs based on various biological and physical principles, and discusses their fundamental advantages and drawbacks for subsequent cellular and molecular assays. Owing to significant genetic heterogeneity among CTCs, microfluidic technologies for isolating individual CTCs have recently been developed. We discuss these single-cell isolation methods, as well as approaches to overcoming the limitations of current microfluidic CTC isolation technologies. Finally, we provide an overview of future innovative microfluidic platforms.
This paper references
Comparison of Two Types of Liquid Biopsies in Patients With Hepatocellular Carcinoma Awaiting Orthotopic Liver Transplantation.
M. I. Sánchez-Lorencio (2015)
Detection of circulating tumor cells in blood using an optimized density gradient centrifugation.
R. Gertler (2003)
Establishment and characterization of a cell line from human circulating colon cancer cells.
Laure Cayrefourcq (2015)
Electrospun TiO2 nanofiber-based cell capture assay for detecting circulating tumor cells from colorectal and gastric cancer patients.
Nangang Zhang (2012)
Detection of EpCAM-Negative and Cytokeratin-Negative Circulating Tumor Cells in Peripheral Blood
S. Mikolajczyk (2011)
Isolation by size of epithelial tumor cells : a new method for the immunomorphological and molecular characterization of circulatingtumor cells.
G. Vona (2000)
Mutational Analysis of Circulating Tumor Cells Using a Novel Microfluidic Collection Device and qPCR Assay.
W. Harb (2013)
Negative enrichment of circulating tumor cells using a geometrically activated surface interaction chip.
Kyung-A Hyun (2013)
Dielectrophoresis has broad applicability to marker-free isolation of tumor cells from blood by microfluidic systems.
Sangjo Shim (2013)
Filtration Parameters Influencing Circulating Tumor Cell Enrichment from Whole Blood
F. Coumans (2013)
Expression of stem cell and epithelial-mesenchymal transition markers in primary breast cancer patients with circulating tumor cells
S. Kasimir-Bauer (2011)
Relationship of circulating tumor cells to tumor response, progression-free survival, and overall survival in patients with metastatic colorectal cancer.
S. Cohen (2008)
Classification of large circulating tumor cells isolated with ultra-high throughput microfluidic Vortex technology
James Che (2016)
Enhanced Isolation and Release of Circulating Tumor Cells Using Nanoparticle Binding and Ligand Exchange in a Microfluidic Chip.
Myoung-Hwan Park (2017)
Highly efficient capture of circulating tumor cells by using nanostructured silicon substrates with integrated chaotic micromixers.
S. Wang (2011)
Selective isolation of magnetic nanoparticle-mediated heterogeneity subpopulation of circulating tumor cells using magnetic gradient based microfluidic system.
Bongseop Kwak (2017)
Isolation of tumor cells using size and deformation.
H. Mohamed (2009)
Enrichment of cancer cells from whole blood using a microfabricated porous filter.
E. H. Kim (2013)
Membrane microfilter device for selective capture, electrolysis and genomic analysis of human circulating tumor cells.
Siyang Zheng (2007)
Dual-patterned immunofiltration (DIF) device for the rapid efficient negative selection of heterogeneous circulating tumor cells.
J. Bu (2016)
Highly efficient single cell arraying by integrating acoustophoretic cell pre-concentration and dielectrophoretic cell trapping.
S. H. Kim (2015)
A single inlet two-stage acoustophoresis chip enabling tumor cell enrichment from white blood cells.
M. Antfolk (2015)
Ex vivo culture of circulating tumor cells using magnetic force‐based coculture on a fibroblast feeder layer
S. Yamamoto (2016)
A radial flow microfluidic device for ultra-high-throughput affinity-based isolation of circulating tumor cells.
Vasudha Murlidhar (2014)
Blood-based analyses of cancer: circulating tumor cells and circulating tumor DNA.
D. Haber (2014)
UV activation of polymeric high aspect ratio microstructures: ramifications in antibody surface loading for circulating tumor cell selection.
J. M. Jackson (2014)
An integrated optofluidic platform for Raman-activated cell sorting.
A. Y. Lau (2008)
Microfluidic magnetophoretic separations of immunomagnetically labeled rare mammalian cells.
T. P. Forbes (2012)
Versatile label free biochip for the detection of circulating tumor cells from peripheral blood in cancer patients.
S. J. Tan (2010)
An index for characterization of nanomaterials in biological systems.
Xin-Rui Xia (2010)
Inertial Focusing for Tumor Antigen–Dependent and –Independent Sorting of Rare Circulating Tumor Cells
E. Ozkumur (2013)
High purity microfluidic sorting and analysis of circulating tumor cells: towards routine mutation detection.
Julien Autebert (2015)
A. Gross (2013)
Laser capture microdissection and single-cell RT-PCR without RNA purification.
K. Keays (2005)
Molecular detection of breast cancer cells in the peripheral blood of advanced-stage breast cancer patients using multimarker real-time reverse transcription-polymerase chain reaction and a novel porous barrier density gradient centrifugation technology.
Megan K. Baker (2003)
Global single-cell cDNA amplification to provide a template for representative high-density oligonucleotide microarray analysis
Kazuki Kurimoto (2007)
Tumor metastasis: mechanistic insights and clinical challenges
P. Steeg (2006)
Analysis of circulating tumor DNA to monitor metastatic breast cancer.
Sarah-Jane Dawson (2013)
Shaping acoustic fields as a toolset for microfluidic manipulations in diagnostic technologies
J. Reboud (2012)
Jetting microfluidics with size-sorting capability for single-cell protease detection.
T. Jing (2015)
Immunological properties of engineered nanomaterials
M. Dobrovolskaia (2007)
Nanoporous elements in microfluidics for multiscale manipulation of bioparticles.
Grace D Chen (2011)
Rapid isolation of cancer cells using microfluidic deterministic lateral displacement structure.
Zongbin Liu (2013)
Global cancer statistics, 2012
L. Torre (2015)
TP53 mutations detected in circulating tumor cells present in the blood of metastatic triple negative breast cancer patients
S. Fernandez (2014)
High throughput capture of circulating tumor cells using an integrated microfluidic system.
Zongbin Liu (2013)
Circulating tumor cell microseparator based on lateral magnetophoresis and immunomagnetic nanobeads.
S. Kim (2013)
3D microfilter device for viable circulating tumor cell (CTC) enrichment from blood
S. Zheng (2011)
Inkjet-like printing of single-cells.
A. Yusof (2011)
Functional phenotyping and genotyping of circulating tumor cells from patients with castration resistant prostate cancer.
P. Paris (2009)
Characterization and enumeration of cells secreting tumor markers in the peripheral blood of breast cancer patients.
C. Alix-Panabières (2005)
Microdevice for the isolation and enumeration of cancer cells from blood
S. J. Tan (2009)
Single cell mutational analysis of PIK3CA in circulating tumor cells and metastases in breast cancer reveals heterogeneity, discordance, and mutation persistence in cultured disseminated tumor cells from bone marrow
G. Deng (2013)
Size and deformability based separation of circulating tumor cells from castrate resistant prostate cancer patients using resettable cell traps.
X. Qin (2015)
Sensitive capture of circulating tumour cells by functionalised graphene oxide nanosheets
H. J. Yoon (2013)
Nanoparticle-mediated binning and profiling of heterogeneous circulating tumor cell subpopulations.
R. Mohamadi (2015)
A microfluidic device enabling high-efficiency single cell trapping.
D. Jin (2015)
Circulating tumor cells (CTC) detection: clinical impact and future directions.
P. Paterlini-Bréchot (2007)
Single-Cell Exome Sequencing and Monoclonal Evolution of a JAK2-Negative Myeloproliferative Neoplasm
Yong Hou (2012)
Isolation of circulating tumor cells using a microvortex-generating herringbone-chip
S. Stott (2010)
Highly selective biomechanical separation of cancer cells from leukocytes using microfluidic ratchets and hydrodynamic concentrator.
B. K. Lin (2013)
Highly focused high-frequency travelling surface acoustic waves (SAW) for rapid single-particle sorting.
D. Collins (2016)
Advances of lab-on-a-chip in isolation, detection and post-processing of circulating tumour cells.
L. Yu (2013)
Immunomagnetic nanoscreening of circulating tumor cells with a motion controlled microfluidic system
Y. Huang (2013)
Aptamer-enabled efficient isolation of cancer cells from whole blood using a microfluidic device.
Weian Sheng (2012)
Size-selective collection of circulating tumor cells using Vortex technology.
E. Sollier (2014)
Inkjet-Print Micromagnet Array on Glass Slides for Immunomagnetic Enrichment of Circulating Tumor Cells
P. Chen (2015)
Cascaded spiral microfluidic device for deterministic and high purity continuous separation of circulating tumor cells.
T. Kim (2014)
Acoustophoretic sorting of viable mammalian cells in a microfluidic device.
Allen H. J. Yang (2012)
Separation and detection of rare cells in a microfluidic disk via negative selection.
Chen-Lin Chen (2011)
Microfluidic, label-free enrichment of prostate cancer cells in blood based on acoustophoresis.
Per Augustsson (2012)
HER2 and breast cancer stem cells: more than meets the eye.
H. Korkaya (2013)
Isolation of rare circulating tumour cells in cancer patients by microchip technology
S. Nagrath (2007)
Microscale Magnetic Field Modulation for Enhanced Capture and Distribution of Rare Circulating Tumor Cells
Peng Chen (2015)
Hand-Held and Integrated Single-Cell Pipettes
K. Zhang (2014)
Efficient tumor formation by single human melanoma cells
Elsa Quintana (2008)
Biology, detection, and clinical implications of circulating tumor cells
S. Joosse (2015)
Detection of Circulating Tumor Cells
S. de Wit (2014)
Capture of circulating tumor cells from whole blood of prostate cancer patients using geometrically enhanced differential immunocapture (GEDI) and a prostate-specific antibody.
J. Gleghorn (2010)
Circulating breast cancer cells are frequently apoptotic.
G. Méhes (2001)
A novel method for the in vivo isolation of circulating tumor cells from peripheral blood of cancer patients using a functionalized and structured medical wire
N. Saucedo-Zeni (2012)
Simultaneous capture and in situ analysis of circulating tumor cells using multiple hybrid nanoparticles.
H. Lee (2013)
Aptamer and Antisense-Mediated Two-Dimensional Isolation of Specific Cancer Cell Subpopulations.
Mahmoud Labib (2016)
An integrated on-chip platform for negative enrichment of tumour cells.
Sajay Bhuvanendran Nair Gourikutty (2016)
Single-cell printing based on impedance detection.
J. Schoendube (2015)
Glioblastoma microvesicles transport RNA and protein that promote tumor growth and provide diagnostic biomarkers
J. Skog (2008)
Label-free single-cell separation and imaging of cancer cells using an integrated microfluidic system
M. Antfolk (2017)
Ultra‐Specific Isolation of Circulating Tumor Cells Enables Rare‐Cell RNA Profiling
R. Jack (2016)
Circulating tumor cells: clinical validity and utility
L. Cabel (2017)
Nanoporous micro-element arrays for particle interception in microfluidic cell separation.
Grace D Chen (2012)
Immunomagnetic techniques for the enrichment and detection of isolated breast carcinoma cells in bone marrow and peripheral blood.
B. Naume (1997)
Polymer nanofiber-embedded microchips for detection, isolation, and molecular analysis of single circulating melanoma cells.
Shuang Hou (2013)
Enhanced and Differential Capture of Circulating Tumor Cells from Lung Cancer Patients by Microfluidic Assays Using Aptamer Cocktail.
L. Zhao (2016)
Velocity valleys enable efficient capture and spatial sorting of nanoparticle-bound cancer cells.
J. Besant (2015)
An integrated microfluidic chip for one-step isolation of circulating tumor cells
Tae Yoon Lee (2017)
NanoVelcro Chip for CTC enumeration in prostate cancer patients.
Yi-Tsung Lu (2013)
High gradient magnetic cell separation with MACS.
S. Miltenyi (1990)
A combined micromagnetic-microfluidic device for rapid capture and culture of rare circulating tumor cells.
J. H. Kang (2012)
Versatile immunomagnetic nanocarrier platform for capturing cancer cells.
Chun-hsien Wu (2013)
A novel microfluidic platform for size and deformability based separation and the subsequent molecular characterization of viable circulating tumor cells
G.E. Hvichia (2016)
Monolithic Chip for High-throughput Blood Cell Depletion to Sort Rare Circulating Tumor Cells
Fabio Fachin (2017)
Evaluation of a Panel of Tumor Markers for Molecular Detection of Circulating Cancer Cells in Women with Suspected Breast Cancer
M. Reinholz (2005)
Slanted spiral microfluidics for the ultra-fast, label-free isolation of circulating tumor cells.
M. Warkiani (2014)
Intratumor heterogeneity and branched evolution revealed by multiregion sequencing.
M. Gerlinger (2012)
Single-Cell Isolation of Circulating Tumor Cells from Whole Blood by Lateral Magnetophoretic Microseparation and Microfluidic Dispensing.
Jinho Kim (2016)
Circulating tumor cell technologies
Meghaan M. Ferreira (2016)
Nanotextured substrates with immobilized aptamers for cancer cell isolation and cytology
Yuan Wan (2012)
Biomimetic nanowire coatings for next generation adhesive drug delivery systems.
K. Fischer (2009)
A novel microchannel-based device to capture and analyze circulating tumor cells (CTCs) of breast cancer
R. Riahi (2014)
Molecular analysis of circulating tumour cells—biology and biomarkers
M. Krebs (2014)
Size-selective microcavity array for rapid and efficient detection of circulating tumor cells.
M. Hosokawa (2010)
Tumor Cells Circulate in the Peripheral Blood of All Major Carcinomas but not in Healthy Subjects or Patients With Nonmalignant Diseases
W. Allard (2004)
High-recovery visual identification and single-cell retrieval of circulating tumor cells for genomic analysis using a dual-technology platform integrated with automated immunofluorescence staining
Daniel E Campton (2015)
Detection and quantification of circulating tumor cells in mouse models of human breast cancer using immunomagnetic enrichment and multiparameter flow cytometry
A. Allan (2005)
Self-seeding microwell chip for the isolation and characterization of single cells.
J. Swennenhuis (2015)
Tunable Thermal-Sensitive Polymer-Graphene Oxide Composite for Efficient Capture and Release of Viable Circulating Tumor Cells.
H. J. Yoon (2016)
Microfluidic sorting of mammalian cells by optical force switching
M. M. Wang (2005)
Ultra-fast, label-free isolation of circulating tumor cells from blood using spiral microfluidics
M. Warkiani (2016)
Highly efficient and selective isolation of rare tumor cells using a microfluidic chip with wavy-herringbone micro-patterned surfaces.
Shunqiang Wang (2016)
Epithelial–Mesenchymal Transition and Stem Cell Markers in Patients with HER2-Positive Metastatic Breast Cancer
A. Giordano (2012)
Three-dimensional nanostructured substrates toward efficient capture of circulating tumor cells.
S. Wang (2009)
Ex vivo culture of circulating breast tumor cells for individualized testing of drug susceptibility
M. Yu (2014)
Circulating cancer cells.
D. Mavroudis (2010)
A cell rolling cytometer reveals the correlation between mesenchymal stem cell dynamic adhesion and differentiation state.
Sungyoung Choi (2014)
Sensitive and high-throughput isolation of rare cells from peripheral blood with ensemble-decision aliquot ranking.
Perry G. Schiro (2012)
Precision Microfilters as an all in one System for Multiplex Analysis of Circulating Tumor Cells.
D. Adams (2016)
Separation of human breast cancer cells from blood by differential dielectric affinity.
F. Becker (1995)
Comprehensive qPCR profiling of gene expression in single neuronal cells
A. Citri (2012)
Detection and quantification of mutations in the plasma of patients with colorectal tumors.
F. Diehl (2005)
On-chip ultrasonic sample preparation for cell based assays†
I. Iranmanesh (2015)
A microfabricated fluorescence-activated cell sorter
Anne Y. Fu (1999)
Technical Insights into Highly Sensitive Isolation and Molecular Characterization of Fixed and Live Circulating Tumor Cells for Early Detection of Tumor Invasion
S. Laget (2017)
Microfluidic device for electric field-driven single-cell capture and activation.
N. M. Toriello (2005)
Quantitative real-time RT-PCR of disseminated tumor cells in combination with immunomagnetic cell enrichment
S. Lankiewicz (2006)
Single Cell Profiling of Circulating Tumor Cells: Transcriptional Heterogeneity and Diversity from Breast Cancer Cell Lines
A. Powell (2012)
Single-cell mRNA sequencing identifies subclonal heterogeneity in anti-cancer drug responses of lung adenocarcinoma cells
K. Kim (2015)
Raman-activated cell sorting based on dielectrophoretic single-cell trap and release.
Peiran Zhang (2015)
In vitro validation of an ultra-sensitive scanning fluorescence microscope for analysis of Circulating Tumor Cells
T. Hillig (2014)
The physics of cancer: the role of physical interactions and mechanical forces in metastasis
D. Wirtz (2011)
Optimization and Evaluation of a Novel Size Based Circulating Tumor Cell Isolation System
L. Xu (2015)
Viable circulating tumour cell detection using multiplex RNA in situ hybridisation predicts progression-free survival in metastatic breast cancer patients
R. E. Payne (2012)
Heterogeneity of PIK3CA mutational status at the single cell level in circulating tumor cells from metastatic breast cancer patients
M. Pestrin (2015)
Circulating tumor cells, disease progression, and survival in metastatic breast cancer.
M. Cristofanilli (2004)
A microfluidic device for label-free, physical capture of circulating tumor cell-clusters
A. F. Sarioglu (2015)
Optimization of ferrofluids and protocols for the enrichment of breast tumor cells in blood
P. Liberti (2001)
Emerging Role of Nanomaterials in Circulating Tumor Cell Isolation and Analysis
H. J. Yoon (2014)
Antibody-independent capture of circulating tumor cells of non-epithelial origin with the ApoStream® system
P. Balasubramanian (2017)
EPISPOT assay: detection of viable DTCs/CTCs in solid tumor patients.
C. Alix-Panabières (2012)
In Vivo Capture of Circulating Tumor Cells Based on Transfusion with a Vein Indwelling Needle.
H. Zhang (2015)
Detection and characterization of circulating tumor cells in blood of primary breast cancer patients by RT-PCR and comparison to status of bone marrow disseminated cells
T. Fehm (2009)
Metastasis: the seed and soil theory gains identity
E. Fokas (2007)
Tumor metastasis: moving new biological insights into the clinic
Liling Wan (2013)
In vitro detection of circulating tumor cells compared by the CytoTrack and CellSearch methods
T. Hillig (2015)
Continuous separation of breast cancer cells from blood samples using multi-orifice flow fractionation (MOFF) and dielectrophoresis (DEP).
Hui-Sung Moon (2011)
Nanomechanical control of cell rolling in two dimensions through surface patterning of receptors.
R. Karnik (2008)
Nanostructure Embedded Microchips for Detection, Isolation, and Characterization of Circulating Tumor Cells
Millicent Lin (2014)
An automated high-throughput counting method for screening circulating tumor cells in peripheral blood.
M. Zhao (2013)
The detection of EpCAM+ and EpCAM– circulating tumor cells
S. Wit (2015)
One-step detection of circulating tumor cells in ovarian cancer using enhanced fluorescent silica nanoparticles
J. Kim (2013)
Hybrid microfluidics combined with active and passive approaches for continuous cell separation
S. Yan (2017)
Dielectrophoretic Separation of Cancer Cells from Blood.
P. Gascoyne (1997)
Modular microsystem for the isolation, enumeration, and phenotyping of circulating tumor cells in patients with pancreatic cancer.
J. Kamande (2013)
Highly efficient capture and enumeration of low abundance prostate cancer cells using prostate‐specific membrane antigen aptamers immobilized to a polymeric microfluidic device
Udara Dharmasiri (2009)
Microfluidic immunocapture of circulating pancreatic cells using parallel EpCAM and MUC1 capture: characterization, optimization and downstream analysis.
Fredrik I. Thege (2014)
Potential of nanofiber matrix as tissue-engineering scaffolds.
Z. Ma (2005)
Continuous Flow Deformability-Based Separation of Circulating Tumor Cells Using Microfluidic Ratchets.
Emily S. Park (2016)
Molecular Biomarker Analyses Using Circulating Tumor Cells
Elizabeth A. Punnoose (2010)
A chip assisted immunomagnetic separation system for the efficient capture and in situ identification of circulating tumor cells.
Man Tang (2016)
HER2 expression identifies dynamic functional states within circulating breast cancer cells
Nicole Vincent Jordan (2016)
Circulating tumor cells predict survival in patients with metastatic prostate cancer.
J. Moreno (2005)
Deterministic separation of cancer cells from blood at 10 mL/min
Kevin Loutherback (2012)
Ultrasensitive Clinical Enumeration of Rare Cells ex Vivo Using a Micro-Hall Detector
D. Issadore (2012)
Semiautomated isolation and molecular characterisation of single or highly purified tumour cells from CellSearch enriched blood samples using dielectrophoretic cell sorting
D. Peeters (2013)
A simple microfluidic dispenser for single-microparticle and cell samples.
A. Kasukurti (2014)
Cell separation using tilted-angle standing surface acoustic waves
X. Ding (2014)
Quantitative analysis of gene expression in a single cell by qPCR
Kiyomi Taniguchi (2009)
Isolating highly enriched populations of circulating epithelial cells and other rare cells from blood using a magnetic sweeper device
Amirali H. Talasaz (2009)
Technologies for Single-Cell Isolation
Andre Gross (2015)
Antibody-independent isolation of circulating tumor cells by continuous-flow dielectrophoresis.
Sangjo Shim (2013)
Circulating tumor cells: liquid biopsy of cancer.
C. Alix-Panabières (2013)
Microfluidic platform for negative enrichment of circulating tumor cells
Bhuvanendran Nair Gourikutty Sajay (2014)
Specific capture and release of circulating tumor cells using aptamer-modified nanosubstrates.
Q. Shen (2013)
Multivalent DNA nanospheres for enhanced capture of cancer cells in microfluidic devices.
Weian Sheng (2013)
Detecting Circulating Tumor Cells: Current Challenges and New Trends
Bin Hong (2013)
Cell separation by dielectrophoretic field-flow-fractionation.
X. Wang (2000)
Non-invasive analysis of acquired resistance to cancer therapy by sequencing of plasma DNA
M. Murtaza (2013)
mRNA-Seq of Single Prostate Cancer Circulating Tumor Cells Reveals Recapitulation of Gene Expression and Pathways Found in Prostate Cancer
G. Cann (2012)
Circulating tumor-cell detection and capture using microfluidic devices
L. Hajba (2014)
Electronic sorting and recovery of single live cells from microlitre sized samples.
Alexandra B Fuchs (2006)
Filter Characteristics Influencing Circulating Tumor Cell Enrichment from Whole Blood
F. Coumans (2013)
Capture and stimulated release of circulating tumor cells on polymer-grafted silicon nanostructures.
Shuang Hou (2013)
Circulating Tumor Cells: Clinically Relevant Molecular Access Based on a Novel CTC Flow Cell
Jessamine Winer-Jones (2014)
Portable Filter-Based Microdevice for Detection and Characterization of Circulating Tumor Cells
H. Lin (2010)
Cancer: Organ-seeking vesicles
J. Rak (2015)
Complex tumor genomes inferred from single circulating tumor cells by array-CGH and next-generation sequencing.
E. Heitzer (2013)
Enrichment with anti-cytokeratin alone or combined with anti-EpCAM antibodies significantly increases the sensitivity for circulating tumor cell detection in metastatic breast cancer patients
G. Deng (2008)
Micro+nanotexturing of substrates to enhance ligand-assisted cancer cell isolation.
M. A. I. Mahmood (2014)
Detection of circulating tumor cells in blood of metastatic breast cancer patients using a combination of cytokeratin and EpCAM antibodies
U. Weissenstein (2011)
Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture
Zuzana Svobodová (2014)
Magneto-controllable capture and release of cancer cells by using a micropillar device decorated with graphite oxide-coated magnetic nanoparticles.
Xiaolei Yu (2013)
Microfluidic technologies in cell isolation and analysis for biomedical applications.
Jing Wu (2017)
Nanowire substrate-based laser scanning cytometry for quantitation of circulating tumor cells.
S. Lee (2012)
Chaotic Mixer for Microchannels
A. Stroock (2002)
Epithelial–mesenchymal transitions in tumour progression
J. Thiery (2002)
Microfluidic sorting and multimodal typing of cancer cells in self-assembled magnetic arrays
A. Saliba (2010)
Clinically relevant microfluidic magnetophoretic isolation of rare-cell populations for diagnostic and therapeutic monitoring applications.
Brian D. Plouffe (2012)
Chip integrated strategies for acoustic separation and manipulation of cells and particles.
T. Laurell (2007)
Cell sorting by deterministic cell rolling.
Sungyoung Choi (2012)
Label-free isolation of prostate circulating tumor cells using Vortex microfluidic technology
Corinne Renier (2017)
Efficient Removal of Platelets from Peripheral Blood Progenitor Cell Products Using a Novel Micro-Chip Based Acoustophoretic Platform
J. Dykes (2011)
Development of a New Rapid Isolation Device for Circulating Tumor Cells (CTCs) Using 3D Palladium Filter and Its Application for Genetic Analysis
Akiko Yusa (2014)
Microfluidic, marker-free isolation of circulating tumor cells from blood samples
N. M. Karabacak (2014)
Enzymatic cleavage of uracil-containing single-stranded DNA linkers for the efficient release of affinity-selected circulating tumor cells.
S. V. Nair (2015)
Microsieve lab-chip device for rapid enumeration and fluorescence in situ hybridization of circulating tumor cells.
Li Shi Lim (2012)
Nanostructured substrates for isolation of circulating tumor cells.
Lixue Wang (2013)
Fluorescent magnetic nanoparticles for magnetically enhanced cancer imaging and targeting in living subjects.
Aihua Fu (2012)
Development of an Automated and Sensitive Microfluidic Device for Capturing and Characterizing Circulating Tumor Cells (CTCs) from Clinical Blood Samples
Priya Gogoi (2016)
Clinical validity of circulating tumour cells in patients with metastatic breast cancer: a pooled analysis of individual patient data.
F. Bidard (2014)
Dynamic single cell culture array.
D. Di Carlo (2006)
Validation of a multiparameter flow cytometry method for the determination of phosphorylated extracellular‐signal‐regulated kinase and DNA in circulating tumor cells
D. Pluim (2012)
Circulating tumor cells as early predictors of metastatic spread in breast cancer patients with limited metastatic dissemination
M. Giuliano (2014)
Continuous particle separation in spiral microchannels using Dean flows and differential migration.
A. Bhagat (2008)
A new device for rapid isolation by size and characterization of rare circulating tumor cells.
Isabelle Desitter (2011)
An integrated microfluidic platform for negative selection and enrichment of cancer cells
Wen-yi Luo (2015)
Rare cell isolation and analysis in microfluidics.
Y. Chen (2014)
Microchip-based immunomagnetic detection of circulating tumor cells.
Kazunori Hoshino (2011)
Self-assembled magnetic filter for highly efficient immunomagnetic separation.
D. Issadore (2011)
Isolation and enrichment of urologic tumor cells in blood samples by a semi-automated CD45 depletion autoMACS protocol.
A. Meye (2002)
O. Farokhzad (2004)
Efficient capture of circulating tumor cells with a novel immunocytochemical microfluidic device.
Mary Nora Dickson (2011)
Modeling and development of a low frequency contactless dielectrophoresis (cDEP) platform to sort cancer cells from dilute whole blood samples.
M. Sano (2011)
Detection of Circulating Tumor Cells in Peripheral Blood of Patients with Metastatic Breast Cancer: A Validation Study of the CellSearch System
S. Riethdorf (2007)
Going with the Flow: From Circulating Tumor Cells to DNA
F. Bidard (2013)
Single-cell analysis and sorting using droplet-based microfluidics
L. Mazutis (2013)
Expected clinical applications of circulating tumor cells in breast cancer.
Youngjin Park (2011)
Oligonucleotide aptamers: A next-generation technology for the capture and detection of circulating tumor cells.
David D. Dickey (2016)
Imaging cytometry for counting circulating tumor cells: comparative analysis of the CellSearch vs ImageStream systems
N. López-Riquelme (2013)
Combination of immunomagnetic enrichment with multiplex RT-PCR analysis for the detection of disseminated tumor cells.
V. Zieglschmid (2005)
M. Toner (2005)
Silicon-based microfilters for whole blood cell separation
H. M. Ji (2008)
Exosomes: current knowledge of their composition, biological functions, and diagnostic and therapeutic potentials.
A. Vlassov (2012)
Comparison of two density gradient centrifugation systems for the enrichment of disseminated tumor cells in blood.
R. Rosenberg (2002)
High-throughput size-based rare cell enrichment using microscale vortices.
Soojung Claire Hur (2011)
Acoustic separation of circulating tumor cells
Peng Li (2015)
ApoStream(™), a new dielectrophoretic device for antibody independent isolation and recovery of viable cancer cells from blood.
V. Gupta (2012)
Multimarker Analysis of Circulating Tumor Cells in Peripheral Blood of Metastatic Breast Cancer Patients: A Step Forward in Personalized Medicine
Andreia de Albuquerque (2012)
Tracking the dynamics of circulating tumour cell phenotypes using nanoparticle-mediated magnetic ranking.
Mahla Poudineh (2017)
The systematic study of circulating tumor cell isolation using lithographic microfilters.
D. Adams (2014)
Separable Bilayer Microfiltration Device for Viable Label-free Enrichment of Circulating Tumour Cells
Ming-Da Zhou (2014)
Circulating tumor cells versus tumor-derived cell-free DNA: rivals or partners in cancer care in the era of single-cell analysis?
E. Kidess (2013)
Hydrodynamic gating valve for microfluidic fluorescence-activated cell sorting.
P. Chen (2010)
Circulating Breast Tumor Cells Exhibit Dynamic Changes in Epithelial and Mesenchymal Composition
M. Yu (2013)
Challenges in circulating tumour cell research
C. Alix-Panabières (2014)
Isolation of rare cells from cell mixtures by dielectrophoresis
P. Gascoyne (2009)
Capture, release and culture of circulating tumor cells from pancreatic cancer patients using an enhanced mixing chip.
Weian Sheng (2014)
Microfluidic enrichment for the single cell analysis of circulating tumor cells
Trifanny Yeo (2016)
Profiling Functional and Biochemical Phenotypes of Circulating Tumor Cells Using a Two-Dimensional Sorting Device.
Mahla Poudineh (2017)
Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood of cancer patients
Zhian Liu (2010)
Establishment of a multimarker qPCR panel for the molecular characterization of circulating tumor cells in blood samples of metastatic breast cancer patients during the course of palliative treatment
M. Bredemeier (2016)
Human mammalian cell sorting using a highly integrated micro-fabricated fluorescence-activated cell sorter (microFACS).
S. H. Cho (2010)
FAST: Size-Selective, Clog-Free Isolation of Rare Cancer Cells from Whole Blood at a Liquid-Liquid Interface.
Tae-Hyeong Kim (2017)
Single-cell sequencing-based technologies will revolutionize whole-organism science
Ehud Shapiro (2013)
Nanotextured polymer substrates show enhanced cancer cell isolation and cell culture.
M. Islam (2015)
A disposable microfluidic device with a reusable magnetophoretic functional substrate for isolation of circulating tumor cells.
Hyungseok Cho (2017)
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