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A Two-enzyme Cascade Reaction Consisting Of Two Reaction Pathways. Studies In Bulk Solution For Understanding The Performance Of A Flow-through Device With Immobilised Enzymes

Nicolas Ghéczy, Kai Sasaki, Makoto Yoshimoto, Sajad Pour-Esmaeil, Martin Kröger, Pasquale Stano, Peter Walde

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A cascade reaction catalysed by bovine carbonic anhydrase (BCA) and horseradish peroxidase (HRP) proceeds over two possible pathways, which explains differences in product formation for differently immobilised enzymes in flow-through reactions.