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Gene Structure, Expression And Chromosomal Localization Of Murine Theta Class Glutathione Transferase MGSTT1-1.

A. Whittington, V. Vichai, G. Webb, R. Baker, W. Pearson, P. Board
Published 1999 · Biology, Medicine

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We have isolated and characterized a cDNA and partial gene encoding a murine subfamily 1 Theta class glutathione transferase (GST). The cDNA derived from mouse GSTT1 has an open reading frame of 720 bp encoding a peptide of 240 amino acids with a calculated molecular mass of 27356 Da. The encoded protein shares only 51% deduced amino acid sequence identity with mouse GSTT2, but greater than 80% deduced amino acid sequence identity with rat GSTT1 and human GSTT1. Mouse GSTT1-1 was expressed in Escherichia coli as an N-terminal 6x histidine-tagged protein and purified using immobilized-metal affinity chromatography on nickel-agarose. The yield of the purified recombinant protein from E. coli cultures was approx. 14 mg/l. Recombinant mouse GSTT1-1 was catalytically active towards 1, 2-epoxy-3-(p-nitrophenoxy)propane, 4-nitrobenzyl chloride and dichloromethane. Low activity towards 1-menaphthyl sulphate and 1-chloro-2,4-dinitrobenzene was detected, whereas mouse GSTT1-1 was inactive towards ethacrynic acid. Recombinant mouse GSTT1-1 exhibited glutathione peroxidase activity towards cumene hydroperoxide and t-butyl hydroperoxide, but was inactive towards a range of secondary lipid-peroxidation products, such as the trans-alk-2-enals and trans,trans-alka-2,4-dienals. Mouse GSTT1 mRNA is most abundant in mouse liver and kidney, with some expression in intestinal mucosa. Mouse GSTT1 mRNA is induced in liver by phenobarbital, but not by butylated hydroxyanisole, beta-napthoflavone or isosafrole. The structure of mouse GSTT1 is conserved with that of the subfamily 2 Theta class GST genes mouse GSTT2 and rat GSTT2, comprising five exons interrupted by four introns. The mouse GSTT1 gene was found, by in situ hybridization, to be clustered with mouse GSTT2 on chromosome 10 at bands B5-C1. This region is syntenic with the location of the human Theta class GSTs clustered on chromosome 22q11.2. Similarity searches of a mouse-expressed sequence tag database suggest that there may be two additional members of the Theta class that share 70% and 88% protein sequence identity with mouse GSTT1, but less than 55% sequence identity with mouse GSTT2.
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This paper is referenced by
10.1080/10408444.2019.1692191
The mercapturic acid pathway
P. E. Hanna (2020)
10.1007/s12041-017-0819-2
Association of GSTM1, GSTT1 and GSTP1 Ile105Val polymorphisms with clinical response to imatinib mesylate treatment among Malaysian chronic myeloid leukaemia patients
Siti Maziras Makhtar (2017)
RESEARCHARTICLE Association of GSTM 1 , GSTT 1 and GSTP 1 Ile 105 Val polymorphisms with clinical response to imatinib mesylate treatment among Malaysian chronic myeloid leukaemia patients
Siti Maziras Makhtar (2017)
Investigating the dual function of the chloride intracellular ion channel proteins
H. A. Khamici (2015)
10.1517/17425255.2013.780027
Utilization of glutathione S-transferase Mu 1- and Theta 1-null mice as animal models for absorption, distribution, metabolism, excretion and toxicity studies
Shingo Arakawa (2013)
10.1097/FPC.0b013e3283527c02
PharmGKB summary: very important pharmacogene information for GSTT1
C. Thorn (2012)
10.1124/dmd.111.042911
Evaluation of Hepatic Glutathione Transferase Mu 1 and Theta 1 Activities in Humans and Mice Using Genotype Information
S. Arakawa (2012)
10.1007/s11033-012-1802-x
Association of CYP1A1, GSTM1, and GSTT1 gene polymorphism with risk of oral submucous fibrosis in a section of North Indian population
T. Ghosh (2012)
and Mice Using Genotype Information
Shingo Arakawa (2011)
10.1016/j.ejogrb.2011.09.012
Association of glutathione S-transferase M1 and T1 null polymorphisms with the development of cervical lesions: a meta-analysis.
Y. Sui (2011)
10.1371/journal.pone.0029366
Association of a Deletion of GSTT2B with an Altered Risk of Oesophageal Squamous Cell Carcinoma in a South African Population: A Case-Control Study
M. Matejcic (2011)
10.1007/S12349-009-0070-3
Effect of acrylamide on chick embryonic liver glutathione S-transferases
Ruxana Begum Sheikh (2010)
10.11606/T.5.2010.TDE-10032010-111801
Genes de reparo do DNA e de susceptibilidade genética em pacientes com melanoma maligno
F. Gonçalves (2010)
10.1089/ars.2007.1994
Redox potential and the response of cardiac ryanodine receptors to CLIC-2, a member of the glutathione S-transferase structural family.
Chris Jalilian (2008)
Phase II enzyme genotypes and their toxicologically relevant phenotypes.
A. Hirvonen (2008)
10.1107/S1744309107049159
Expression, purification, crystallization and preliminary X-ray diffraction analysis of chloride intracellular channel 2 (CLIC2).
B. Cromer (2007)
10.1124/dmd.107.017905
Generation and Functional Characterization of Mice with a Disrupted Glutathione S-Transferase, Theta 1 Gene
K. Fujimoto (2007)
10.1093/AJE/KWJ321
Meta- and pooled analysis of GSTT1 and lung cancer: a HuGE-GSEC review.
S. Raimondi (2006)
10.1016/J.PESTBP.2005.10.004
Studies on glutathione transferase from grasshopper (Zonocerus variegatus)
I. O. Adewale (2006)
10.1042/BJ20042113
A recently identified member of the glutathione transferase structural family modifies cardiac RyR2 substate activity, coupled gating and activation by Ca2+ and ATP.
A. Dulhunty (2005)
10.1016/J.CANLET.2004.06.016
Glutathione S-transferase genotypes and cancer risk.
F. Parl (2005)
10.1016/J.CHEMBIOL.2005.07.007
A chemical inhibitor reveals the role of Raf kinase inhibitor protein in cell migration.
Shoutian Zhu (2005)
10.1023/A:1026750431055
Inhibition of platelet function by GSTM1-null human peripheral lymphocytes exposed to benzo(a)pyrene-induced challenge
I. Onaran (2004)
10.1016/J.BIOCEL.2004.01.026
CLIC-2 modulates cardiac ryanodine receptor Ca2+ release channels.
P. Board (2004)
10.1097/00008571-200402000-00005
Functional polymorphism of human glutathione transferase A2.
N. Tetlow (2004)
Sequential action of phase I and II enzymes cytochrome p450 1B1 and glutathione S-transferase P1 in mammary estrogen metabolism.
D. Hachey (2003)
10.1042/BJ20030415
Biochemical and genetic characterization of a murine class Kappa glutathione S-transferase.
I. R. Jowsey (2003)
10.1042/BJ20011878
Identification and characterization of GSTT3, a third murine Theta class glutathione transferase.
M. Coggan (2002)
Maternal and Newborn Polymorphisms in Phase I/II Metabolic Genes Contribute to Risk of Adverse Reproductive Outcomes
T. Nukui (2002)
10.1006/TAAP.2002.9348
Direct comparison of the nature of mouse and human GST T1-1 and the implications on dichloromethane carcinogenicity.
P. Sherratt (2002)
10.1007/978-94-010-0357-5
Biotechnology for the Environment: Strategy and Fundamentals
S. Agathos (2002)
10.1007/978-94-010-0357-5_7
Coping with a Halogenated One-Carbon Diet: Aerobic Dichloromethane-Mineralising Bacteria
S. Vuilleumier (2002)
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