← Back to Search
Chitosan Induces Ca2+-mediated Programmed Cell Death In Soybean Cells
Anna Zuppini, B. Baldan, R. Millioni, F. Favaron, L. Navazio, P. Mariani
Published 2004 · Biology
Download PDFAnalyze on Scholarcy
Summary • Chitosan, a component of the cell wall of many fungi, has been widely used to mimic pathogen attack and has been shown to induce several defence responses. • Here we show that low concentrations (50 µg ml − 1 ) of chitosan are able to induce an increase in cytosolic Ca 2+ concentration ([Ca 2+ ] cyt ), accumulation of H 2 O 2 in the culture medium, induction of the defence gene chalcone synthase ( chs ), and cell death in soybean cells ( Glycine max ). • Chitosan-induced cell death occurred through cytoplasmic shrinkage, chromatin condensation and activation of caspase 3-like protease, suggesting the activation of a programmed cell death (PCD) pathway. Buffering extracellular Ca 2+ with the Ca 2+ chelator EGTA prevents [Ca 2+ ] cyt elevation, H 2 O 2 production and all downstream PCD features, but not cell death. • Higher doses (200 µg ml − 1 ) of chitosan evoked neither Ca 2+ transient and H 2 O 2 production nor caspase 3-like activation, but caused cell death, possibly as a result of plasma membrane disturbance.
This paper references
Unraveling regulatory networks in plant defense using microarrays
O. Rowland (2001)
Oligosaccharide signalling for defence responses in plant
N. Shibuya (2001)
Host-parasite interactions: elicitation of defense responses in plants with chitosan.
Hadwiger La (1999)
Elicitor-Stimulated Induction of Defense Mechanisms and Defense Gene Activation in Grapevine Cell Suspension Cultures
V. Řepka (2004)
Do Plant Caspases Exist?
E. Woltering (2002)
Dissection of the ozone-induced calcium signature.
H. Clayton (1999)
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
A tomato homologue of the human protein PIRIN is induced during programmed cell death
D. Orzáez (2004)
Death Don't Have No Mercy: Cell Death Programs in Plant-Microbe Interactions.
J. Dangl (1996)
An improved method for monitoring cell death in cell suspension and leaf disc assays using evans blue
C. Jacyn Baker (2004)
Oligogalacturonides and chitosan activate plant defensive genes through the octadecanoid pathway.
S. Doares (1995)
Receptor-mediated activation of a plant Ca2+-permeable ion channel involved in pathogen defense.
S. Zimmermann (1997)
Harpin and hydrogen peroxide both initiate programmed cell death but have differential effects on defence gene expression in Arabidopsis suspension cultures.
R. Desikan (1998)
Developmentally regulated conversion of surface‐exposed chitin to chitosan in cell walls of plant pathogenic fungi
N. Gueddari (2002)
High affinity binding of a fungal oligopeptide elicitor to parsley plasma membranes triggers multiple defense responses
T. Nürnberger (1994)
Induction of cysteine and serine proteases during xylogenesis in Zinnia elegans
Z. Ye (2004)
A chalcone synthase with an unusual substrate preference is expressed in barley leaves in response to UV light and pathogen attack
A. B. Christensen (2004)
Elicitor-stimulated ion fluxes and O2- from the oxidative burst are essential components in triggering defense gene activation and phytoalexin synthesis in parsley.
T. Jabs (1997)
Chemical-induced apoptotic cell death in tomato cells: involvement of caspase-like proteases
Anke J. de Jong (2000)
Role of Calcium in Signal Transduction during the Hypersensitive Response Caused by Basidiospore-Derived Infection of the Cowpea Rust Fungus
Haixin Xu (1998)
Fusarium solani DNase is a signal for increasing expression of nonhost disease resistance response genes, hypersensitivity, and pisatin production.
L. Hadwiger (1995)
Signals controlling the expression of cytosolic ascorbate peroxidase during pathogen-induced programmed cell death in tobacco
R. Mittler (2004)
Pathogen-induced programmed cell death in tobacco.
R. Mittler (1997)
Zinc induces mixed types of cell death, necrosis, and apoptosis, in molt-4 cells.
M. Hamatake (2000)
Calcium signaling and cytotoxicity.
G. E. Kass (1999)
Resistance response physiology and signal transduction.
D. Scheel (1998)
The RPM1 plant disease resistance gene facilitates a rapid and sustained increase in cytosolic calcium that is necessary for the oxidative burst and hypersensitive cell death.
M. Grant (2000)
Constitutive caspase-like machinery executes programmed cell death in plant cells
M. Elbaz (2002)
Tracheary element differentiation uses a novel mechanism coordinating programmed cell death and secondary cell wall synthesis
Interaction of nuclear proteins with intrinsically curved DNA in a matrix attachment region of a tobacco gene
Y. Fukuda (2004)
Cleavage of lamin‐like proteins in in vivo and in vitro apoptosis of tobacco protoplasts induced by heat shock
H. Chen (2000)
Regulation and execution of programmed cell death in response to pathogens, stress and developmental cues.
E. Beers (2001)
Callose Biosynthesis as a Ca2+-Regulated Process and Possible Relations to the Induction of Other Metabolic Changes
H. Kauss (1985)
The Involvement of Cysteine Proteases and Protease Inhibitor Genes in the Regulation of Programmed Cell Death in Plants
M. Solomon (1999)
Dopaminergic neurotoxins, 6,7-dihydroxy-1-(3′,4′-dihydroxybenzyl)-isoquinolines, cause different types of cell death in SH-SY5Y cells: apoptosis was induced by oxidized papaverolines and necrosis by reduced tetrahydropapaverolines
W. Maruyama (2000)
Independent pathways leading to apoptotic cell death, oxidative burst and defense gene expression in response to elicitin in tobacco cell suspension culture.
M. Sasabe (2000)
Calcium-mediated apoptosis in a plant hypersensitive disease resistance response
A. Levine (1996)
Cleavage of Nuclear DNA into Oligonucleosomal Fragments during Cell Death Induced by Fungal Infection or by Abiotic Treatments.
D. Ryerson (1996)
Plant programmed cell death: a common way to die.
A. Danon (2000)
Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: A novel, rapid defense response
D. Bradley (1992)
Store-operated Ca2+ entry: dynamic interplay between endoplasmic reticulum, mitochondria and plasma membrane.
A. Parekh (2003)
The presence and subcellular localization of caspase 3‐like proteinases in plant cells
H. Korthout (2000)
Die and let live - programmed cell death in plants.
E. Lam (1999)
The ultrastructure of chilling stress
H. Kratsch (2000)
Elicitation of lignin biosynthesis and isoperoxidase activity by pectic fragments in suspension cultures of castor bean.
R. H. Bruce (1989)
Effect of Chitosan on Systemic Viral Infection and Some Defense Responses in Potato Plants
S. Chirkov (2004)
Transgenic aequorin monitors cytosolic calcium transients in soybean cells challenged with β-glucan or chitin elicitors
A. Mithöfer (1999)
Nonhost resistance and nonspecific plant defenses.
M. Heath (2000)
The degrees of polymerization and N-acetylation of chitosan determine its ability to elicit callose formation in suspension cells and protoplasts of Catharanthus roseus
H. Kauss (2004)
Effect of different gravity environments on DNA fragmentation and cell death in Kalanchoe leaves.
M. C. Pedroso (2000)
Programmed cell death of tracheary elements as a paradigm in plants
H. Fukuda (2004)
Defense on multiple fronts: how do plants cope with diverse enemies?
Changes in gene expression during programmed cell death in tomato cell suspensions
F. Hoeberichts (2004)
Transfected Aequorin in the Measurement of Cytosolic Ca2+ Concentration ([Ca2+]c)
M. Brini (1995)
Induction of chalcone synthase expression in white spruce by wounding and jasmonate.
S. Richard (2000)
The role of calcium in oligogalacturonide-activated signalling in soybean cells
L. Navazio (2002)
Programmed cell death in cell cultures
P. McCabe (2004)
H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response
Alex Levine (1994)
 Ferrous ion oxidation in presence of ferric ion indicator xylenol orange for measurement of hydroperoxides
S. Wolff (1994)
Effects of supplied cinnamic acids and biosynthetic intermediates on the anthocyanins accumulated by wild carrot suspension cultures
D. C. Baker (2004)
Induced plant responses to pathogen attack
R. Welle (1991)
Lipid rafts and T cell receptor signaling: a critical re‐evaluation
P. Pizzo (2002)
Involvement of poly(ADP‐ribose) polymerase and activation of caspase‐3‐like protease in heat shock‐induced apoptosis in tobacco suspension cells
R. H. Tian (2000)
Identification, characterization, and purification of a tobacco endonuclease activity induced upon hypersensitive response cell death.
R. Mittler (1995)
Chitosan Induces Apoptosis via Caspase‐3 Activation in Bladder Tumor Cells
M. Hasegawa (2001)
Defense response in slash pine: chitosan treatment alters the abundance of specific mRNAs.
M. Mason (1997)
The Wellcome Prize Lecture
A. Parekh (2003)
Proteases for Cell Suicide: Functions and Regulation of Caspases
H. Chang (2000)
An Apoplastic Ca2+ Sensor Regulates Internal Ca2+ Release in Aequorin-transformed Tobacco Cells*
S. Cessna (2001)
Analysis and Effects of Cytosolic Free Calcium Increases in Response to Elicitors in Nicotiana plumbaginifolia Cells Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.005579.
D. Lecourieux (2002)
The Quantitative Relation Between Plant and Bacterial Cells Involved in the Hypersensitive Reaction
J. G. Turner (1974)
This paper is referenced by
Multiple effects of chitosan on plant systems: solid science or hype.
L. Hadwiger (2013)
Chitosan as a MAMP, searching for a PRR
M. Iriti (2009)
Thaxtomin A induces programmed cell death in Arabidopsis thaliana suspension-cultured cells
I. Duval (2005)
Pathogenesis in Mosses
M T Lawton (2009)
Size, acetylation and concentration of chitooligosaccharide elicitors determine the switch from defence involving PAL activation to cell death and water peroxide production in Arabidopsis cell suspensions
J. C. Cabrera (2006)
Carbohydrate polymers exhibit great potential as effective elicitors in organic agriculture: A review.
Fang Zheng (2020)
Metabolic Pathways Regulated by Chitosan Contributing to Drought Resistance in White Clover.
Z. Li (2017)
Chitosan in Plant Protection
Abdelbasset El Hadrami (2010)
Chitosan oligosaccharides affect xanthone and VOC biosynthesis in Hypericum perforatum root cultures and enhance the antifungal activity of root extracts
C. Badiali (2018)
Signal role for activation of caspase-3-like protease and burst of superoxide anions during Ce4+-induced apoptosis of cultured Taxus cuspidata cells
Z. Ge (2005)
The plant proteolytic machinery and its role in defence.
R. V. D. van der Hoorn (2004)
Transcriptomic Analysis of Avocado Hass (Persea americana Mill) in the Interaction System Fruit-Chitosan-Colletotrichum
Luis-Ángel Xoca-Orozco (2017)
Defense/stress responses activated by chitosan in sycamore cultured cells
M. Malerba (2011)
Chitosan oligosaccharides modulate the supramolecular conformation and the biological activity of oligogalacturonides in Arabidopsis.
J. Cabrera (2010)
Chitosan Effects on Plant Systems
M. Malerba (2016)
Cadmium toxicity in cultured tomato cells—Role of ethylene, proteases and oxidative stress in cell death signaling
E. Iakimova (2008)
Synthesis and characterization of chitosan nanoparticles and their effect on Fusarium head blight and oxidative activity in wheat.
A. Kheiri (2017)
Enhancing phytochemical levels, enzymatic and antioxidant activity of spinach leaves by chitosan treatment and an insight into the metabolic pathway using DART-MS technique.
S. Singh (2016)
Identification of Cell Apoptosis Morphology Induced by Oligosaccharides
Hong Guo (2011)
Biological control of fusarium seedling blight disease of wheat and barley.
M. Khan (2006)
Double Layer Coatings: A New Technique for Maintaining Physico-Chemical Characteristics and Antioxidant Properties of Dragon Fruit During Storage
A. Ali (2013)
Cell death-mediated antiviral effect of chitosan in tobacco.
M. Iriti (2006)
Elicited Teucrium chamaedrys cell cultures produce high amounts of teucrioside, but not the hepatotoxic neo-clerodane diterpenoids.
F. Antognoni (2012)
Potential benefits of foliar application of chitosan and Zinc in tomato
A. Salimi (2019)
Proline and chitosan enhanced efficiency of microspore embryogenesis induction and plantlet regeneration in Brassicanapus L.
B. Ahmadi (2015)
The Role of Ethylene and Calcium in Programmed Cell Death of Cold-Stored Cucumber Fruit
Jingxin Chen (2014)
Chitosan-induced antiviral activity and innate immunity in plants
M. Iriti (2014)
Oligochitosan induces programmed cell death in tobacco suspension cells
H. Zhang (2012)
Chemical- and Pathogen-Induced Programmed Cell Death in Plants
E. Iakimova (2005)
Monitoring programmed cell death triggered by mild heat shock in soybean-cultured cells.
Anna Zuppini (2006)
Signal transduction events in aluminum-induced cell death in tomato suspension cells.
E. Yakimova (2007)
The effect of chitosan–PMAA–NPK nanofertilizer on Pisum sativum plants
Noha S Khalifa (2018)See more